Chd7 (NM_001277149) Mouse Untagged Clone

CAT#: MC229764

Chd7 (untagged) - Mouse chromodomain helicase DNA binding protein 7 (Chd7)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chd7
• Synonyms: A730019I05Rik; Cy; Cyc; Cycn; Cyn; Dz; Edy; F; Flo; GENA 47; Gena 52; GENA 60; L; Lda; metis; Mt; Ob; Obt; To; Todo; WBE1; Wh; Whi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229764 representing NM_001277149
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCCAGGGATGATGAGTCTTTTTGGCGAGGATGGGAGTCTGTTCAGCGAAGGCCTCGAAGGCC
  TCGGGGAATGTGGCTACCCCGAAAACCCCGTGAACCCCATGGGTCAGCAGATGCCCATAGACCAAGGTTT
  TCCCTCCTTACAGCCATCCCTCCACCATCCTTCACCTAATCAGAATCAAACCAAGTTGACCCATTTTGAT
  CACTATAGTCAGTATGAACAGAAGATGCATCTGATGGATCAGCCTAACCGAATGATGGGCAGCGCCCCCG
  GGAACGGATTGGCGTCTCCGCACTCACAGTATCACACCCCTCCCGTCCCCCAGGTGCCCCACGGAGGCGG
  TGGTGGCGGTCAGATGGGAGTCTATCCCGGCATCCAGAACGAAAGGCACGGGCAATCTTTTGTGGATGGC
  GGCTCCATGTGGGGCCCCCGGGCCGTTCAGGTACCAGACCAGATACGAGCCCCCTACCAGCAGCAGCAGC
  CGCAGCCGGCTCCTTCGGGGCCCCCGGCACAGGGCCACCCCCAGCACATGCAGCAGATGGGCAGCTATCT
  GGCACGTGGGGATTTCTCCATGCAGCAGCACGGCCAGCCACAGCAGAGGATGGGCCAGTTTTCCCAAGGC
  CAAGAGGGCCTCAGTCAGGGAAGCCCTTTCATTGCCACCTCAGGACCCGGCCACCTGTCGCACATGCCCC
  AGCAGAGCCCCAGCATGGCCCCTTCCCTGCGCCACCCAGTGCAGCAGCAGTTCCACCATCACCCCGCTGC
  TCTCCACGGAGAATCCGTTGCCCACAGTCCCAGATTCTCCCCCAACCCTCCTCAACAAGGGGCTGTCAGG
  CCACAAACCCTTAACTTTAGTTCTCGGAACCAGACAGTCCCCTCCCCTACTGTAAACAACTCAGGGCAGT
  ATTCTCGATATCCTTACAGTAACCTAAATCAGGGATTAGTTAACAGTACAGGGATGAATCAAAATTTAGG
  CCTTACAAACAGTACTCCAATGAATCAGTCGGTACCAAGATACCCCAATGCCGTGGGATTCCCGTCAAAC
  AGTGGTCAGGGACTCGTGCACCAGCAGCCTATCCACTCCAGTGGCTCACTGAACCAAATGAACACACAAA
  CTATGCACCCTTCACAGCCTCAGGGAACGTACGCCTCTCCACCTCCCATGTCACCCATGAAAGCAATGAG
  TAACCCAGCAGGCACGCCGCCTCCGCAGGTCAGGCCCGGAAGTGCTGGGATGCCCATGGAAGTTGGCAGT
  TATCCAAATATGCCCCACCCTCAGCCATCTCACCAGCCCCCTGGTGCCATGGGAATCGGACAGAGGAATA
  TGGGCCCCAGAAACATGCAGCAGCCTCGTTCGTTTATGGGCATGTCCTCGGCACCCAGGGAGCTGACCGG
  ACACATGAGACCAAATGGTTGCCCTGGTGTCGGCCTTGCGGATCCACAAGCAATCCAGGAACGACTGATA
  CCTGGCCAACAGCACCCTGGTCAGCAGCCATCTTTCCAGCAGTTGCCAACCTGTCCTCCACTACAGCCCC
  ACCCAGGCCTGCACCAGTCTTCACCTCCACATCCCCATCACCAGCCTTGGGCACAACTCCACCCGTCACC
  CCAGAATACCCCACAGAAAGTGCCTGTGCATCAGCATTCTCCATCGGAGCCCTTTCTAGAGAAGCCAGTG
  CCGGATATGACCCAGGTCAGCGCACAGAACGCTCAGCTAGTGAAGAGCGACGATTACCTGCCGTCGATAG
  AGCAGCAGCCACAGCAGAAGAAGAAGAAAAAGAAAAACAACCACATTGCTGCAGGGGATTCCAGTAAAGG
  TTTTGGTAAAGATGACTTCCCTGGTGGGGTTGAAAACCAAGAACTGCGCAGGAACTCACTGGATGTGTCC
  CAGGAAGAAAAAAAGAAAAAGAAAAGGCCGAAGGTCAAAAAAGATCCGAAGGAATCGAAGGAGCCGAAGG
  AGAAGAAAGAGCCCAAGACCCCAAAAGCCCCGAAGATCCCCAAAGAGCCAAAGGAAAAGAAAGCAAAAAC
  TGTCACGCCAAAACCCAAATCCAGCAAAAAGTCAAGTAATAAGAAACCGGATTCAGAAGCAAGTGCTTTG
  AAGAAGAAGGTCAACAAAGGAAAAACAGAAGGTTCTGAAAATTCAGACTTGGACAAAACACCCCCACCGT
  CCCCCGCTCCTGAAGAGGACGAAGACCCAGGTGTCCAGAAGAGGCGGTCCAGCAGGCAGGTGAAGAGGAA
  GCGATACACTGAAGATTTGGAGTTCAAGATTTCTGATGAGGAGGCAGATGATGCAGATGCTGCTGGAAGA
  GACTCCCCATCCAACACCTCGCAGTCAGAGCAGCAGGAATCTGTTGATGCAGAAGGCCCAGTGGTGGAGA
  AGATTATGAGCAGCCGCCTAGTGAAAAAGCAGAAGGAATCTGGCGAGGAGGTAGAAGTTGAGGAATTTTA
  TGTGAAGTACAAAAACTTCTCCTATCTTCATTGTCAGTGGGCGTCAGTAGAAGATCTGGAAAAAGACAAG
  AGAATTCAGCAAAAGATAAAACGGTTTAAGTCAAAACAGGGCCAGAGCAAATTCCTCTCAGAGATTGAGG
  ATGACCTTTTTAATCCAGACTATGTGGAAGTTGACCGGATAATGGACTTTGCACGTAGCACAGATGACCG
  GGGCGAGCCTGTGATTCACTACCTGGTGAAGTGGTGCTCACTCCCTTATGAAGACAGCACATGGGAGCTG
  AAGCAGGACATAGATCAAACAAAGATCGAAGAGTTTGAAAAACTCATGTCCAGGGAGCCGGAAACCGAGC
  GTGTGGAGCGACCTCCTGCTGATGACTGGAAGAAATCGGAGAGTTCCAGGGAGTATAAAAACAATAACAA
  ACTCAGGGAATACCAGTTGGAGGGAGTAAACTGGCTACTTTTCAATTGGTACAACATGCGAAACTGCATT
  TTAGCAGATGAGATGGGTTTGGGAAAAACGATCCAGTCCATTACGTTTCTCTATGAGATATACTTGAAAG
  GAATCCATGGCCCCTTTTTAGTAATTGCCCCATTGTCCACGATCCCCAACTGGGAAAGGGAATTCCGAAC
  CTGGACGGAGTTGAACGTGGTTGTGTACCATGGGAGCCAAGCCAGCCGTCGGACCATTCAGTTGTATGAA
  ATGTACTTCAAAGATCCCCAGGGTCGAGTGATAAAGGGGTCCTATAAGTTCCACGCCATCATCACTACAT
  TCGAGATGATTCTGACGGACTGTCCTGAGCTGCGTAATATTCCTTGGCGCTGTGTGGTCATCGATGAAGC
  CCACAGGCTGAAGAACAGAAACTGCAAGCTGCTGGAAGGGCTGAAGATGATGGACTTGGAACACAAAGTC
  CTGCTGACCGGGACCCCGCTGCAGAACACTGTGGAAGAGCTCTTCAGCCTGCTTCATTTCTTGGAGCCAA
  GTCGCTTCCCTTCAGAAACAACCTTTATGCAAGAATTTGGTGATCTGAAAACGGAAGAGCAGGTGCAAAA
  ACTACAGGCTATTCTAAAGCCAATGATGTTGAGACGCCTGAAAGAGGATGTAGAAAAGAACTTGGCCCCC
  AAAGAAGAGACTATTATTGAAGTTGAGCTAACAAACATTCAGAAGAAATATTACCGAGCCATCCTTGAGA
  AGAATTTTACATTTCTTTCCAAAGGTGGTGGACAAGCTAACGTACCTAACCTTTTAAACACTATGATGGA
  ACTGAGAAAATGCTGCAATCATCCCTACCTAATCAATGGTGCTGAAGAGAAAATTTTGGAAGAGTTTAAA
  GAAACACACAATGCAGAGTCTCCAGATTTTCAGCTCCAGGCAATGATCCAGGCTGCTGGCAAGCTGGTGC
  TGATTGACAAGCTGCTGCCAAAGCTGAAGGCTGGTGGCCACAGGGTGCTTATCTTCTCCCAGATGGTGCG
  CTGCTTGGACATCCTGGAAGACTATCTCATCCAGAGACGGTACCCATACGAAAGGATTGATGGCCGGGTC
  AGAGGCAACCTCCGCCAGGCAGCTATTGACAGATTCTCCAAACCTGATTCTGATAGGTTTGTGTTCCTCC
  TGTGCACACGGGCAGGAGGGCTAGGCATTAACCTCACTGCTGCTGATACCTGCATCATCTTTGATTCAGA
  CTGGAATCCCCAGAATGACCTCCAGGCTCAAGCTAGATGCCACAGAATAGGGCAGAGCAAATCTGTGAAA
  ATCTACAGGCTGATTACAAGGAACTCCTATGAAAGGGAGATGTTTGACAAGGCTAGTCTGAAGCTGGGTC
  TGGATAAAGCCGTGTTGCAGTCCATGAGTGGAAGAGAAAATGCCACTAACGGGGTACAACAGCTGTCCAA
  GAAGGAAATAGAAGACCTCCTGCGAAAGGGGGCATATGGTGCACTTATGGATGAAGAAGATGAAGGGTCT
  AAGTTTTGTGAAGAGGATATTGACCAGATTCTCCTTCGACGAACCCACACTATCACCATTGAGTCTGAAG
  GAAAAGGCTCCACATTTGCTAAGGCCAGCTTTGTTGCGTCTGGAAATAGGACAGACATTTCTTTGGATGA
  CCCAAATTTCTGGCAGAAGTGGGCTAAGAAGGCTGAATTGGACATCGATGCCTTAAACGGGAGGAACAAC
  CTGGTTATTGACACTCCTCGAGTGAGGAAGCAGACCCGGCTATACAGCGCAGTGAAGGAAGACGAACTGA
  TGGAGTTCTCCGACCTGGAAAGCGACTCCGAAGAAAAGCCTTGTGCAAAGCCACGGCGCCCCCAGGATAA
  GTCTCAGGGCTATGCCCGGAGCGAGTGCTTCAGGGTAGAGAAGAATCTGCTGGTTTACGGCTGGGGCCGC
  TGGACAGATATCCTGTCCCATGGACGCTACAAACGTCAGCTGACTGAGCAAGATGTCGAAACCATCTGCA
  GAGCGATCCTGGTGTACTGTCTCAACCACTACAGAGGGGACGAGAACATCAAAAGCTTTATCTGGGACCT
  CATCACACCCACGGCGGATGGCCAGACTCGGGCCCTGCTAAATCATTCCGGTCTGTCGGCCCCTGTGCCG
  AGGGGCCGGAAGGGGAAGAAGGTGAAGGCCCAGAGTACGCAGCCAGTAGTGCACGATGCTCACTGGCTGG
  CAAGCTGCAACCCAGACGCCCTGTTCCAGGAGGACAGCTACAAGAAGCACCTGAAGCACCACTGCAACAA
  GGTCCTGCTGCGTGTCCGCATGCTGTACTACCTAAGACAAGAAGTGATAGGAGACCAGGCAGAGAAGATC
  CTCGAGGGCGCTGACTCAAGTGAAGCAGACGTGTGGATCCCTGAGCCATTCCATGCTGAGGTTCCTACAG
  ATTGGTGGGACCGAGAAGCAGACAAATCCCTCCTAATTGGAGTGTTTAAACATGGCTATGAGAAGTACAA
  TTCCATGCGAGCTGACCCTGCACTGTGCTTCCTGGAACGAGTCGGAATGCCCGATGCCAAGGCCATCGCT
  GCCGAGCAGAGAGGGACAGACATGCTAGCAGATGGTGGTGACGGTGGAGAATTTGATAGAGAAGATGAAG
  ATCCTGAATATAAACCAACGAGAGCACCATTCAAAGATGAAATAGATGAATTTGCGAATTCTCCCGCAGA
  GGATAAGGAAGAGTCCATGGAGGTGCACAGCTCAGGCAAGCACAGCGAGAGCAATGCTGAGTTAGGTCAG
  CTCTACTGGCCGAACACGTCAACCCTGACTACGCGTCTGCGCCGGCTCATTACTGCCTATCAGCGCAGCT
  ATAAAAGGCAGCAAATGAGGCAAGAGGCCCTAATGAAGACGGACCGGCGCAGACGGAGGCCCCGGGAGGA
  AGTGAGAGCGCTGGAGGCAGAAAGGGAAGCTATTATATCTGAGAAAAGGCAAAAGTGGACGAGAAGAGAA
  GAAGCGGACTTTTACCGGGTGGTATCCACCTTCGGGGTTATTTTTGACCCAGTGAAACAGCAGTTTGATT
  GGAACCAATTCCGAGCCTTTGCCAGACTAGACAAAAAGTCTGATGAGAGCCTGGGGAAGTATTTCAGCTG
  CTTTGTGGCCATGTGCAGACGGGTGTGTCGGATGCCTGCCAAGCCCGAGGACGAGCCACCTGACCTCGCC
  TCCCTTATCGAGCCCATCACGGAGGAACGAGCATCCAGGACACTGTACCGCATTGAACTGCTTCGCAAGA
  TCCGAGAGCAGGTCCTTCATCACCCACAACTAAGTGACAGGCTGAAGCTCTGCCAGCCCAGCCTAGATCT
  GCCCGAGTGGTGGGAATGTGGACGGCACGACAGGGACCTGCTGGTGGGTGCTGCTAAGCACGGGGTCAGC
  CGGACAGACTATCACATCCTCAACGACCCTGAGTTGTCCTTCTTAGATGCACACAAAAGCTTTGCTCAGA
  ACCGTGGGGCCAGTACCGTCCCTCCTTTGAATACTCTGGCCATGGGCTTTGGCCAGACTCCTCCAGTCAT
  TTCCTCTGCTCACGTTCACGAAGAAAAGGCAATGGAGCAGGCTGAAGGCAAGGCAGAGGAGTGTGAACAC
  TCACCGGCCAAAGAGAGGTCGGATGGGAAAGAGGAGGAAGAAGAAGCAGGCGGGGCCAAGGATGGCAAGC
  AGGAATGTGAGGTCGAGGCCAGCTCTGTGAAGGGTGAACTCAAAGGGGTGGAGGGCAGTGCAGACCCTGG
  GTCCAAGTCTGTCTCAGAGAAGGGCTCAGAAGAGGACGAAGAAGAGAAGCTGGAGGACGATGATAAGTCG
  GAGGAGTCGTCGCAGCCAGAAGCAGGAGCCGTCTCGAGAGGGAAGACTTTCGATGAAGAAAGCAATGCTT
  CTCTGAGCACTGCTAGGGATGAAACCCGAGATGGATTCTACATGGAGGATGGAGATGCTTCGGTGGCTCA
  GCTCCTTCATGAACGAACATTTGCCTTCTCCTTTTGGCCCAAGGACAGAGTAATGATAAACCGATTAGAC
  AACATCTGTGAAGCTGTGTTGAAAGGCAAATGGCCAGTAAATCGGCGCCAGATGTTTGATTTCCAGGGCC
  TTGTCCCTGGGTACCCACCCTCCGCCGTGGACAGTCCCCTGCAGAAGAGGAGCTTTGCCGAGCTCTCTAT
  GGTCAGCCAGGCCAGCATCAGTGCGAGCGAGGACATCACCACGTCTCCTCAGTTGTCCAAGGATGATGCC
  CTCAACCTGTCTGTCCCTCGCCAGCGGCGGCGGAGGAGAAGGAAAGTTGAAATCGAGGCTGAAAGAGCTG
  CCAAGAGGAGAAACCTTATGGAGATGGTTGCCCAGCTTCGAGAGTCTCAGGTGGTCTCAGAAAATGGACA
  AGAAAAAGTTGTGGACTTATCAAAGGCCTCGAGAGAGGCAACAAGCTCTACCTCAAATTTCTCATCTCTT
  ACTTCAAAGTTTATCTTGCCTAATGTCTCCACGCCAGTGTCTGATGCCTTTAAGTCTCAGATGGAGCTGC
  TCCAAGCAGGCCTTTCACGCACACCCACAAGGCATATGCTCAATGGCTCCCTGGTGGATGGAGAGCCCCC
  CATGAAGAGGAGGCGAGGCAGGAGGAAAAACGTGGAGGGCCTCGACCTACTTTTCATGAGCCACAAACGG
  ACGCCGTTAAGTGCAGAGGATGCTGAGGTGACCAAAGCTTTTGAAGAAGATATAGAGACCCCACCAATAA
  GAAACATTCCTTCTCCTGGACAGCTGGACCCCGACACCCGGATCCCTGTGATAAACCTTGAAGATGGGAC
  TAGGCTGGTGGGAGAAGACGCTCCTAAAAACAAGGACTTGGTTGACTGGCTGAAGCTGCACCCCACCTAC
  ACTGTCGATATGCCGAGTTATGTACCAAAGAACACAGACGTGCTGTTTTCCTCGTTTCAGAAACCGAAAC
  AGAAACGACATAGATGTCGAAATCCTAATAAATTGGATATTAACACTTTGACAGGAGAAGAAAGGGTTCC
  TGTTGTTAACAAACGAAATGGGAAGAAGATGGGTGGAGCCATGGCCCCTCCAATGAAGGACCTGCCCAGG
  TGGCTGGAGGAGAACCCTGAGTTTGCTGTCGCTCCAGACTGGACCGATATAGTCAAGCAGTCTGGTTTTG
  TTCCCGAGTCAATGTTTGACCGTCTCCTCACTGGACCTGTGGTGCGGGGAGAAGGAGCCAGCAGGAGAGG
  ACGGAGGCCCAAGAGCGAGATCGCCCGAGCAGCCGCGGCGGCTGCTGCTGTCGCCTCTACTTCAGGGATC
  AACCCTCTGTTGGTGAACAGCCTGTTTGCTGGGATGGACCTGACAAGCCTTCAGAATCTCCAGAACCTCC
  AGTCCCTCCAGCTGGCAGGGCTCATGGGCTTCCCTCCAGGACTGGCCACAGCTGCCACCGCCGGAGGCGA
  TGCGAAGAGCCCCGCCGCAGTGCTGCCCCTGATGCTGCCAGGGATGGCGGGCCTGCCCAACGTGTTCGGC
  TTGGGCGGGCTGCTGAACAACCCTCTCTCAGCTGCTACTGGAAACACCACTACTGCTTCAAGTCAAGGAG
  AGCCAGAGGATGGCACTTCCAAAGCGGAAGAGAAAGGAAACGACAATGAAGACGAGAACCGAGACTCTGA
  GAAAAGCACAGACACCGTTTCGGCTGCTGACTCCGCGAACGGATCTGTTGGTGCTGCTACTGCCCCGGCT
  GCGTTACCCTCCAACCCGCTGGCCTTCAACCCTTTCCTCCTGTCCACCATGGCCCCAGGCCTCTTCTACC
  CATCCATGTTTCTACCTCCAGGACTGGGGGGATTGACACTGCCTGGCTTCCCAGCACTGGCGGGACTCCA
  AAACGCCGTGGGTACCAGCGAAGAAAAGGCTGCTGACAAGGCCGAGGGAGGGCCCTGTAAAGACGGAGAG
  ACCCTGGAGGGCAGCGATGCCGAGGAGAACCTGGACAAGACTGTAGAGTCCTCCATCTTGGAAGACGAGG
  TTGCACAGGGTGAAGAGCTGGACTCCCTCGAAGGCGGGGATGAGATAGAAAACACCGGAAACGACGAGTA
  A
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001277149
• Insert Size: 8961 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277149.1, NP_001264078.1
• RefSeq Size: 11306 bp
• RefSeq ORF: 8961 bp
• Locus ID: 320790
• UniProt ID: A2AJK6
• Cytogenetics: 4 3.68 cM
• Gene Summary: This gene encodes a protein containing two chromodomains and an ATP-binding helicase domain that functions as a regulator of transcription. Mutations in this gene result in an array of development defects, including inner ear problems. Mice defective for this gene exhibit many of the clinical features of the CHARGE syndrome caused by mutations in the homologous gene in human. [provided by RefSeq, Sep 2015]