Kmt2b (NM_001290573) Mouse Untagged Clone

CAT#: MC229760

Kmt2b (untagged) - Mouse lysine (K)-specific methyltransferase 2B (Kmt2b), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kmt2b
• Synonyms: 2610014H22Rik; mKIAA0304; Mll2; Wbp7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229760 representing NM_001290573
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGGCGGCGGCAGTTGCCCCGGGCCTGGCTCCGCACGGGGCCGCTTCCCGGGCCGGC
  CGCGGGGTTCCGGCGGGGGCGGGGGCCGCGGCGGCCGAGGCAACGGAGCCGAAAGAGTGCGGGTAGCCCT
  GCGGCGCGGTGGCGGCGCGGCGGGGCCGGGAGGAGCCGAGCCCGGGGAGGACACGGCCCTGCTCCGTTTG
  CTGGGTCTTCGCCGGGGCCTGCGCCGGCTCCGCCGCCTGTGGGCTGGTGCGCGAGTTCAGCGAGGCCGAG
  GCCGCGGCCGGGGACGGGGCTGGGGCCCGAACCGAGGCTGCATGCCGGAGGAAGAGAGCAGTGACGGGGA
  ATCCGAGGAGGAGGAGTTTCAGGGTTTTCATTCAGATGAAGATGTGGCCCCCAGTTCCCTGCGCTCTGCG
  CTTCGGTCCCAGCGAGGTCGAGCCCCTCGGGGTCGGGGCCGCAAGCATAAGACGACCCCCCTTCCTCCTC
  GCCTAGCAGATGTGACTCCTGTCCCCCCAAAGGCCCCTACTCGGAAACGGGGTGAGGAGGGCACAGAACG
  GATGGTGCAGGCACTGACTGAACTTCTCCGGCGGTCCCAAGCACCCCAACCCCCCCGGAGCCGGGCACGG
  GCACGTGAACCCTCTACTCCCCGACGGTCTCGGGGAAGGCCCCCAGGACGGCCAGCCGGTCCCTGCCGGA
  AAAAGCAGCAAGCAGTAGTGTTAGCAGAAGCGGCTGTGACAATCCCTAAACCCGAGCCTCCGCCCCCTGT
  GGTTCCAGTAAAGAACAAAGCTGGCAGTTGGAAATGCAAGGAGGGACCTGGTCCAGGACCTGGAACCCCC
  AAACGTGGAGGACAGCCTGGGAGAGGAGGCCGTGGAGGCAGGGGCAGAGGCCGAGGCGGACTTCCCCTTA
  TGATCAAGTTTGTTTCCAAGGCCAAGAAAGTGAAGATGGGACAATTGTCCCAGGAACTAGAATCAGGTCA
  GGGTCATGGTCAACGTGGGGAAAGCTGGCAGGATGCCCCCCAAAGAAAAGATGGAGATGAACCAGAAAGG
  GGCTCGTGTAGAAAGAAACAGGAGCAGAAACTGGAGGAGGAGGAGGAGGAGGAGGAGAAGGAGGGAGAAG
  AGAAGGAAGAAAAAGATGACAATGAGGATAACAACAAGCAAGAGGAAGAGGAGGAGACTGAGAGAGCTGT
  GGCTGAGGAAGAAGCGATGCTAGCCAAGGAGAAGGAAGAGGCAAAGCTTCCGTCACCACCCTTGACTCCT
  CCAGTCCCTTCACCACCTCCTCCTCTACCACCCCCTTCAACATCTCCTCCACCTCCAGCATCCCCTCTAC
  CACCCCCAGTTTCTCCTCCTCCCCCACTTTCCCCTCCCCCATATCCAGCCCCAGAGAAGCAGGAAGAGTC
  TCCTCCTCTTGTCCCAGCTACATGCTCTAGGAAGAGGGGCAGGCCTCCCCTGACTCCTAGCCAGCGGGCA
  GAGAGGGAAGCTGCTCGGTCAGGACCAGAGGGCACCCTTTCTCCCACTCCAAACCCTAGCACCACCACAG
  GAAGCCCTTTGGAAGACAGTCCTACTGTGGTCCCCAAAAGTACCACCTTTTTAAAGAATATACGGCAGTT
  TATTATGCCTGTAGTGAGTGCTCGCTCCTCCCGTGTTATCAAGACACCCCGGCGATTTATGGATGAAGAT
  CCTCCCAAACCTCCAAAGGTGGAGGCTTCTATTGTTCGACCTCCTGTTGCAACCTCCCCACCTGCTCCCC
  AGGAACCAGTACCAGTCTCCTCTCCACCTCGTGTCCCAACTCCCCCATCTACCCCAGTCCCACTCCCTGA
  GAAGAGGCGGTCTATCCTAAGGGAACCCACATTTCGCTGGACCTCACTAACCCGGGAGCTGCCGCCACCT
  CCACCTGCTCCTCCACCAGCCCCATCTCCACCACCTGCCCCTGCCACTCCCTCCCGGAGGCCCTTGCTCC
  TTCGGGCCCCTCAGTTTACCCCAAGTGAAGCCCACCTGAAGATCTATGAATCGGTGCTTACCCCTCCTCC
  TCTTGGGGCTCTTGAAACCCCTGAGCCAGAGCTGCCTCCTGCTGATGACTCTCCAGCAGAGCCTGAGCCT
  AGGGCAGTGGGGCGGACCAATCACCTCAGCTTGCCTCGCTTTGTCCCTGTGGTCACCAGTCCTGTTAAGG
  TTGAGGTGCCCCCCCATGGAGCTCCAGCTCTGAGTGAAGGCCAGCAGCTTCAGCTCCAGCAGCCCCCGCA
  GGCCTTGCAGACCCAGCTCCTACCCCAGGCACTACCACCACAGCAACCACAAGCGCAGCCACCTCCATCA
  CCACAACACACACCACCACTGGAAAAGGCCCGTGTTGCAAGTCTGGGTTCCCTGCCACTATCTGGGGTGG
  AGGAGAAAATGTTTAGCCTTCTTAAGAGAGCTAAGGTGCAGTTATTCAAGATCGATCAACAGCAACAGCA
  GAAAGTGGCGGCTTCAATGCCGCTAAGCCCTGCAGTACAAACCGAGGAGGCTGTGGGGACTGTCAAACAG
  ACCCCGGACAGAGGCTGTGTCAGGTCTGAAGATGAATCCATGGAAGCTAAGAGAGATCGAGCCTCGGGCC
  CTGAGTCACCTTTACAAGGCCCCCGAATCAAACATGTCTGCCGTCACGCTGCTGTGGCCTTGGGTCAGGC
  TCGGGCCATGGTGCCTGAAGATGTACCCCGCCTCAGTGCCCTCCCTCTCCGGGATCGGCAGGACCTCGCC
  ACTGAGGATACGTCATCAGCATCAGAGACTGAGAGTGTCCCTTCCCGATCCCAGCGGGAAAAGGTGGAAT
  CTGCAGGGCCTGGGGGAGACTCGGAGCCTACAGGGTCTACAGGGGCCCTGGCCCACACACCACGGCGGTC
  ACTGCCATCCCACCATGGCAAGAAGATGAGAATGGCTCGTTGTGGGCACTGTCGGGGTTGCTTGCGTGTG
  CAGGACTGTGGCTCTTGTGTCAACTGCTTGGATAAACCTAAATTTGGGGGTCCCAACACTAAGAAGCAGT
  GCTGTGTATACCGGAAGTGTGACAAGATAGAGGCTCGGAAGATGGAGCGGCTGGCAAAAAAAGGCCGGAC
  GATAGTGAAGACGCTGTTGCCCTGGGATTCCGATGAATCTCCTGAGGCCTCCCCTGGTCCTCCAGGCCCA
  CGCCGGGGGGCGGGAGCTGGGGGGTCCCGGGAGGAGGTGGGGGCCACTCCCGGGCCCGAGGAGCAGGACT
  CCCTCCTACTGCAGCGCAAGTCAGCCCGACGCTGCGTCAAACAGCGGCCCTCCTATGATGTCTTCGAGGA
  CTCAGATGACTCAGAGCCCGGGGGTCCTCCTGCTCCAAGACGTCGCACCCCCAGAGAACATGAGCTGCCA
  GTACTAGAACCTGAGGAGCAGAGCCGGCCCCGCAAACCCACCCTGCAGCCTGTGTTGCAGCTCAAGGCCC
  GAAGGCGCCTGGACAAGGATGCTTTAGCCCCTGGCCCCTTTGCCTCTTTTCCCAATGGCTGGACTGGAAA
  GCAGAAGTCTCCTGACGGTGTGCACCGGGTCCGAGTAGATTTTAAGGAGGATTGCGACCTAGAGAATGTG
  TGGCTTATGGGTGGTCTCAGTGTGCTGACTTCTGTGCCAGGGGGGCCACCGATGGTGTGTCTGCTGTGTG
  CTAGCAAAGGCCTCCATGAGCTGGTTTTTTGCCAAGTCTGCTGCGATCCTTTCCACCCCTTCTGTCTGGA
  GGAGGCTGAGCGGCCCTCACCTCAGCATCGTGACACCTGGTGCTGCAGGCGCTGCAAGTTCTGCCATGTT
  TGTGGGCGCAAAGGCCGAGGGTCCAAGCACCTCCTGGAATGTGAACGCTGTCGTCATGCTTACCACCCAG
  CCTGCCTGGGACCCAGCTACCCAACCCGGGCCACGCGTAGACGGCGCCACTGGATCTGTTCAGCCTGTGT
  GCGCTGTAAGAGCTGTGGGGCAACTCCAGGCAAGAACTGGGATGTCGAGTGGTCTGGAGATTACAGCCTC
  TGCCCCAGGTGCACCGAGCTCTATGAAAAAGGGAACTACTGCCCAATTTGCACACGCTGCTATGAAGACA
  ATGACTATGAGAGCAAGATGATGCAGTGTGCACAGTGTGACCACTGGGTGCATGCCAAGTGTGAGGGACT
  CTCAGATGAAGACTACGAGATCCTTTCTGGACTGCCAGACTCAGTGTTGTACACGTGTGGGCCATGTGCT
  GGGGCGACACAGCCCCGCTGGCGAGAGGCCCTGAGTGGGGCCCTGCAGGGAGGCCTGCGTCAGGTGCTCC
  AGGGCCTGTTAAGCTCAAAGGTGGCAGGCCCATTGCTGCTGTGCACCCAGTGTGGGCAGGATGGGAAGCA
  GCTCCACCCAGGACCCTGTGATTTACAAGCTGTGGGAAAGCGCTTTGAGGAAGGTCTCTATAAGTCTGTG
  CACAGCTTCATGGAAGACGTAGTGGCCATCTTGATGAGGCATTCAGAAGAAGGAGAGACCCCAGAGCGCC
  GGGCTGGAAGCCAGATGAAAGGACTCCTACTGAAGCTGCTAGAGTCTGCGTTCTGCTGGTTTGACGCCCA
  TGATCCTAAGTACTGGCGACGGAGTACACGGCTGCCAAACGGAGTGCTCCCCAATGCTGTGTTGCCTCCA
  TCCTTGGACCATGTCTATGCTCAGTGGAGACAACAGGAATCAGAGACCCCAGAATCGGGGCAGCCTCCAG
  GGGATCCTTCAGCAGCTTTCCAGAGCAAGGACCCAGCTGCTTTCTCACACCTGGATGACCCTCGTCAGTG
  TGCACTCTGTCTCAAGTATGGGGATGCTGACTCTAAGGAGGCGGGGCGGCTCCTGTACATTGGGCAGAAT
  GAGTGGACACATGTCAATTGTGCCATTTGGTCAGCTGAAGTGTTTGAGGAGAATGATGGCTCCCTCAAGA
  ACGTCCATGCTGCTGTTGCACGTGGAAGGCAGATGCGCTGTGAGCTCTGCCTCAAGCCTGGAGCCACAGT
  TGGCTGCTGCCTGTCCTCATGTCTCAGCAACTTCCACTTCATGTGTGCCCGGGCCAGCTACTGCATCTTC
  CAGGATGACAAGAAGGTTTTCTGCCAGAAACACACAGACCTCCTAGATGGCAAGGAGATCGTGACCCCTG
  ATGGTTTTGATGTTCTTCGGCGAGTCTATGTGGACTTCGAGGGCATCAACTTCAAGCGAAAGTTCTTGAC
  TGGACTTGAACCAGATGTCATCAATGTACTCATTGGCTCCATCAGAATCAACTCCCTGGGCACCCTATCT
  GATCTCTCAGACTGTGAGGGTCGACTCTTCCCCATTGGCTACCAATGCTCCCGCCTGTATTGGAGCACAG
  TGGATGCTCGGAGGCGCTGCTGGTATCGGTGCCGAATTCTAGAGTATCGCCCTTGGGGGCCAAGGGAGGA
  GCCAGTGCACCTAGAAGCAGCAGAGGAGAACCAGACCATTGTGCATAGCCCAACCCCTTCCTCAGAGCCC
  CCCAATCATGATGATCTGCCAGATACAGATTCTCTGATCCCTGGAGATCCTGTACACCACTCCCCCATTC
  AGAATCTGGATCCCCCGCTTCGGACAGATTCAAGCAATGGCCCTCCTCCCACCCCTCGCTCCTTCTCAGG
  GGCTCGAATCAAAGTGCCCAACTACTCACCGTCTCGGAGGCCCTTAGGGGGAGTCTCTTTTGGTCCTCTG
  CCCTCTCCAGGAAGTCCATCTTCCCTGACCCACCACATCCCTACTGTGGGAGACTCGGACTTTCCAGCTC
  CCCCAAGGCGATCTCGGCGTCCTAGCCCCCTGGCCACCAGGCCACCACCATCCCGGAGGACCTCCTCTCC
  TCTCAGAACTTCCCCTCAGCTCAGGGTGCCCCTGTCTACCTCAGTCACAGCCCTCACACCTACCTCAGGG
  GAGCTGGCTCCCCCTGACCTGGCCCCATCTCCCCTTCCACCGTCTGAAGACCTGGGCCCAGACTTTGAAG
  ACATGGAGGTGGTGTCAGGACTAAGTGCTGCTGACCTGGACTTTGCAGCCAGCCTGCTGGGGACTGAGCC
  TTTCCAGGAAGAGATTGTGGCTGCTGGGGCAGTGGGGAGCAGCCAGGGAGGTCCAGGGGACAGCTCAGAG
  GAGGAGGCCAGCCCTACCACTCACTATGTCCACTTCCCTGTGACCGTGGTGTCTGGCCCTGCCCTGGCCC
  CCAGTTCCCTTGCTGGAGCTCCCCGAATCGAGCAGCTGGACGGAGTGGATGATGGCACAGACAGCGAGGC
  TGAGGCTGTCCAACAACCCCGGGGACAGGGGACTCCTCCTTCAGGACCAGGAGTGGGCCGAGGAGGGGTC
  CTTGGAGCAGCAGGCGACAGAGCCCAACCTCCTGAAGACCTGCCTTCAGAAATTGTGGATTTCGTGTTGA
  AGAACCTAGGGGGTCCTGGGGAGGGGGCTGCTGGCCCCAGAGAGGACTCTCTTCCTTCAGCACCTCCCTT
  GGCTAATGGCAGCCAACCCCCACAAAGCCTGTCCACTAGCCCAGCTGACCCCACCCGAACATTTGCTTGG
  CTCCCTGGAGCTCCAGGGGTCCGGGTATTGAGTCTGGGTCCTGCTCCAGAACCCCCCAAACCTGCCACAT
  CCAAGATCATCCTTGTCAACAAGCTGGGGCAAGTATTTGTGAAGATGGCAGGGGAGGGTGAGCCTGTAGC
  ACCCCCAGTAAAGCAGCCCCCTCTACCCCCCATCATTCCCCCAACAGCTCCCACCTCCTGGACTCTGCCC
  CCAGGACCCCTGCTCAGTGTGTTACCAGTGGTAGGGGTAGGAGTGGTCCGGCCTGCCCCACCCCCACCTC
  CACCCCCACTAACACTGGTGTTTAGCAGTGGGCCCCCCAGCCCACCACGCCAGGCCATTCGTGTCAAGAG
  GGTATCCACCTTCTCTGGCCGATCCCCTCCAGTTCCTCCCCCAAACAAAACTCCCCGGCTGGATGAAGAT
  GGAGAGTCCTTGGAGGATGCCCATCATGTGCCAGGGATCAGTGGCAGTGGGTTCAGTCGTGTTCGCATGA
  AAACGCCCACAGTCCGTGGAGTTCTGGACCTGAACAATCCTGGGGAGCAGCCTGAGGAGGAAAGCCCTGG
  GCGCCCCCAGGACCGGTGTCCTTTGCTGCCACTTGCAGAAGCTCCTTCCCAGGCCCTCGATGGTTCCTCA
  GACCTACTGTTTGAGTCCCAGTGGCATCACTATTCAGCAGGCGAGGCCTCAAGCTCTGAGGAAGAGCCTC
  CATCTCCAGAGGACAAAGAAAACCAGGTCCCCAAACGGGTTGGTCCACACCTGCGCTTTGAGATCAGCAG
  TGATGATGGCTTCAGTGTGGAGGCAGAGAGCTTGGAGGTGGCATGGAGAACTCTGATTGAGAAAGTGCAA
  GAAGCACGAGGGCATGCACGGCTCAGGCATCTCTCCTTTAGTGGAATGAGTGGAGCGCGGCTCCTGGGCA
  TCCACCACGATGCTGTCATCTTCCTGGCAGAGCAGCTCCCTGGGGCCCAGCGCTGCCAGCATTATAAGTT
  CCGCTACCACCAGCAGGGAGAGGGCCAGGAAGAGCCACCTCTGAATCCCCATGGGGCTGCTCGTGCTGAG
  GTCTATCTCCGGAAGTGTACCTTTGACATGTTCAACTTCCTGGCCTCCCAGCACCGTGTGCTCCCCGAGG
  GAGCCACCTGTGATGAAGAAGAGGATGAGGTGCAGCTTCGGTCCACCAGACGTGCCACCAGCCTGGAGCT
  TCCTATGGCCATGCGCTTTCGTCATCTCAAAAAGACATCCAAAGAGGCTGTGGGAGTCTACAGATCAGCT
  ATCCATGGACGAGGTCTGTTTTGTAAACGCAACATCGATGCGGGAGAGATGGTCATTGAGTACTCTGGCA
  TTGTCATTCGCTCTGTGCTGACTGACAAGCGAGAGAAGTTCTATGATGGGAAGGGTATTGGGTGCTACAT
  GTTCCGCATGGATGACTTTGATGTGGTGGACGCCACCATGCACGGCAATGCTGCCCGATTCATCAACCAC
  TCATGTGAGCCCAACTGCTTCTCTCGTGTCATCCATGTGGAAGGCCAGAAACACATTGTCATCTTTGCCT
  TGCGCCGCATCCTGCGAGGTGAGGAGCTCACCTATGACTACAAGTTTCCTATCGAGGATGCCAGCAACAA
  GCTGCCCTGCAACTGTGGCGCCAAGCGCTGCCGTCGCTTTCTTAACTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001290573
• Insert Size: 8169 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290573.1, NP_001277502.1
• RefSeq Size: 8484 bp
• RefSeq ORF: 8169 bp
• Locus ID: 75410
• Cytogenetics: 7 B1
• Gene Summary: Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (By similarity). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.