Wnk1 (NM_001199083) Mouse Untagged Clone

CAT#: MC229755

Wnk1 (untagged) - Mouse WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnk1
• Synonyms: 6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229755 representing NM_001199083
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA
  AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG
  CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC
  ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC
  CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC
  TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC
  CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG
  TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG
  CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG
  GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA
  CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT
  AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT
  GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA
  TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG
  GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT
  AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC
  TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA
  GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT
  CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT
  TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG
  ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA
  GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG
  GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC
  TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT
  AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC
  AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT
  CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT
  GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG
  GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC
  CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA
  GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCGTCGTGGCCGTAGCATGTCGGTTT
  GTGTTCCCCATCTTTCTGCTGTTCCCTCTCTATCCCGCATCTCTCCCAGTGCTCCTTCCACCCCACCACC
  AGTGCTGTCTGCACCTCTTTGTCCTTCCCTCCTTCGGACTGCCCCTGAGGAAACTTTTGCCGAAAAACTC
  TCTAAAGCATTGGAGAGTGTCCTGCCTATGCACTCTGCCTCCCAGCGCAAGCACCGACGCTCCAGCCTGC
  CTTCCCTCTTTGTCACTACTCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATC
  ACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCCACCTGTCCACCGAAA
  GTAGCCATTTCCCAACGACGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCGCCACTTCCAACCCCTGC
  TGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTAGCAGCCCAACTAACTGGACACCAGAGGCCATAGT
  TATGTTGGGTGCTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGATGCAGGTCCAACCTGTGTTTGAG
  CCAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAAGAAGCTCACTATTTTATTCCTC
  AGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCAGGTGGCAGGACAGTATGAGGGCATTTCATA
  CAACTCACCAGTACTGTCAAGTCCTATGAAACAGATATCTGAACAGAAGCCAGTGCCGGGGGGCCCTGCC
  TCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTGGTAGGACACCTTCAGAATTTAAGATTAG
  ATTCTGGACCAAGTCCAGCATCACCCCTCTCTAGTATTTCTGCGCCTAACAGTACAGATGCTACACATCT
  GAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAACTAATAGCAATGAGAACAGAAGC
  AACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCTTCAGGAGAAGGAGGTGGGATTTTACCTCAAC
  GTGTTTACCGAAATCGGCAGGTTGCAGTGGACTCAAATCAGGAAGAACTGTCTCCTCAGTCAGTTGGATT
  ACATTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCATGCCCCCGAATTGACCATTTCTGTG
  GTAGAACCTATGGGACAAATCTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCTCAAATTACTT
  CTTCAGATCTCAGTGATTTTCAATCACCTCCCCCTACAGGGGGGACAGCTGCACCTTTTGGCTCTGACGT
  CTCATTGCCCTTTATTCGCCTGCCTCAGACAGTGTTACAAGAATCCCCACTATTCTTCTGTTTCCCCCAA
  GGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGAGGATCTACACTCCATCCACAGGCAC
  AGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTGGCTGGGGTTCTATCTTCACAACCTATCCAACATCC
  TCAGCAGCAGGGAATACAGCCAACTGTTCCTTCTCAACAGGCAGTACAGTATTCACTTCCACAAGCAGCA
  TCTTCCAGTGAAGGTACTACTGCACAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTACTC
  AGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAATAACACAGTCACAACCTACTCAGCCTGTTCC
  ATTGGTTACCTCTGCAGACAGTGCACATTCAGACGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCT
  CCATCATCCAGCGGGAGGCATGAAGGGAGGACAACAAAACGGCATTATAGAAAATCCGTAAGAAGTCGCT
  CTCGGCATGAAAAGACTTCTCGCCCAAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGT
  AGAATGTCAATTAGAGACTCATAATCGGAAGATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCT
  GAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCTTTTGTGGCTC
  AAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAGTGAGGATGTCAGTGTGGAGCCTGAGGGTGA
  CCAGGGATTGGAGAGTCTGCAAGGAAAGGATGACTATGGCTTCCCAGGTTCTCAGAAATTAGAAGGAGAG
  TTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACGCAAG
  TTGTTCATTCTGCTGGAAGACGGTTTATAGTGAGTCCTGTACCTGAAAGTAGGTTACGAGAATCAAAAGT
  TTTCACCAGTGACATATCAGATCCAGTTGTGGCCTCTACATCTCAGGCCCCTGGAATGAACTTATCTCAC
  TCTGCTTCATCCCTCAGTCTCCAGCAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCA
  ATACAGCACCTCCAAATTTTAATCACATGGCGGGACCAACATTTTCTCCCTTTTTGGCTAGCATTGCTGG
  TGTCCAAACTGTAGCAGCATCCACACCGTCAGTATCAGTCCCTATAACAAGCAGTCCTCTTAATGACATT
  TCCACATCAGTGATGCAGTCTGAGACTGCACTGCCCACTGAAAAAGGCATCGTGGGAGTTACCACTACCA
  GCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACAATGTCTGTGTCTGAGTCACCAACATCTTCCAGTGC
  GGTTTCAAGTTCTACAGTACCTGCAGTTGTCACTGTGTCCACACCATCCCAGCCAGTTCAGGCTTCCACA
  TCTGGGAGCATTGCTTCAAGTACAGGCAGTTTTCCTCCTGGAACATTTTCTACAACTACAGCTACTACAA
  TGGGTAGTGTGGTGGCCCCAGATGCTAAGCCTCCAACTGTATTATTGCAACAGGTAGCAAGCAATACTGC
  TGGGGTTGCCATAGTGACATCAGTCTCTACCACCACTCCATTCCCAGGCATGGCTTCACAGCCATCCCTT
  CCACTTAGTAGCAGCACCTCTGCTCCTACTCTAGCTGAAACAATGGTGGTTAGTGCACACTCACTAGACA
  AGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGTGCGCCATCCTCTTCCTCCTCCTCTGG
  AACAGCTGTGTCTACTTCTGTTTCTCAGCCTGGTATGGTTCATCCTTTGGTCATCTCATCAGCTGTAGTT
  TCTACACCTGGCCTCCCCCAACCAGTAGTACCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCC
  CACCCTTGGTACAACCTGTTGTTAATGTGCCTGCTGTACAGCAAACACTCATTCACAGTCAGCCTCAACC
  AGCTCTGCTTCCCAACCAGCCTCACACACACTGTCCTGAAATGGATGCTGATACCCAATCCAAAGCTCCT
  GGGATTGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCTCTCTTTAGTGAACACAGCTCATCTGGAA
  CTCAGCATGCTTCTGTCTCCCTAGAGACACCTTTAGTAGTAGAGACGACTGTCACACCGGGCATCACTAC
  CACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGTACTTGCTTACCACCAACCAGTTTACCA
  TTAGGAGCAGCTGGCATGCCAGTTATGCCAGTAGGCACACCTGGGCAGGTTTCTACCCCAGGCACACACG
  CTTCAGCCCCAGTCGGCACTGCAACAGGAGTAAAACCTGGAACAACTCCCCCAAAGCCAACTAAGACTGT
  GGTGCCACCAGTGGGTACTGAACTTTCAGCCGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCTGGA
  CCTTCTCTAATTCAGTCCCAGCAACCACTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAG
  AGACTATTACAGTGGCACCTGCAGTTGGTCCTTTGTCTACGATGTCTTCAACAACAGTTACAGAAGCAGG
  GACTCGGCTTCAAAAGGATGGCACAGAAGGTCATGTCACAGCAACTAGTTCAGGAGCTGGTGTTGTTAAG
  ATGGGGCGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGAAAGAACGTAAAAATAGGTCAGAAGATA
  CAAAGTCGGTTCATTTTGAATCTAGCACATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTAC
  CCTGGTAAAGCCAGAGCCTAATGGAATTAGTATTTCTGGCATCTCCTTAGATGTCCCAGACAGCACCCAC
  AAAGCACCTACCCCAGAAGCAAAGTCAGATGCAGGACAGCCTACCAAGGTGGGACGTTTTCAGGTGACAA
  CTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGA
  GGGACCAGTGACCTCTCCTCCTTTCAGAGATTCAGAACAAACTGTTATTCCTGCTGTGATACCAAAGAAG
  GAGAAGCCTGAGCTGGCAGAACCTTCCCATTTGAATGGGCCATCTTCAGACCTGGAGGCTGCCTTTCTAA
  GTAGGGGTACAGAGGATGGCTCAGGTAGTCCACACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCGT
  CCAGAATCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCATACATGAGTAGTGACAATGAGTCCGAC
  ATTGAAGATGAAGACTTAAGGTTAGAACTACGACGACTACGAGAAAAACATCTTAAAGAGATTCAGGACC
  TGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACTAAACTGGGCAAGGTTCCCCCTGCTGTCAT
  TATTCCCCCAGCTGCTCCTCTGTCGGGGAGAAGAAGGAGACCCACTAAAAGCAAAGGCAGCAAGTCTAGT
  CGCAGCAGCTCATTGGGCAATAAAAGCCCACAGCTTTCAGGCAACCTGTCTGGTCAGAGTGGAACTTCAG
  TCTTACACCCCCAACAGACCCTCCATCCCGCAGGAAACACCCCCGAGACTGGGCACAACCAGCTGTTACA
  GCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTA
  CCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAATACCGTTGGAGGAACAGTGAGTAGCCAGG
  CTGCCCAGGCTCAGCCTCCTGCTATGACTTCCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTT
  AGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTAT
  GAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGTGTGTTCCCATGACCTCAAACCTGG
  GTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCACTTCACCAAATCCATGTGTCCCCC
  ACAGCAGTATGGTTTTCCACCTGCCCCATTTGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCGCAG
  CCACTTGGCCAGTTCCAACCTGTAGGAACAGCATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGT
  CCATCAGCAACCCCCCAGGTTCCAATCTACGGACCACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199083
• Insert Size: 7881 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199083.1, NP_001186012.1
• RefSeq Size: 11319 bp
• RefSeq ORF: 7881 bp
• Locus ID: 232341
• UniProt ID: P83741
• Cytogenetics: 6 F1
• Gene Summary: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame compared to variant 5. This variant represents the exon combination of the brain and spinal cord variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 4, which is shorter than isoform 5 but has the same N- and C-termini. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.