Cacna1a (NM_001252059) Mouse Untagged Clone

CAT#: MC229730

Cacna1a (untagged) - Mouse calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (Cacna1a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1a
• Synonyms: alpha1A; APCA; BI; Caca1a; Cacnl1a4; Cav2.1; Ccha1a; EA2; FHM; HPCA; la; MHP; MHP1; nmf352
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229730 representing NM_001252059
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCTTTGGAGACGAGATGCCGGGCCGCTACGGCGCAGGCGGAGGAGGCTCAGGGCCGGCCGCCG
  GGGTGGTCGTGGGCGCCGCGGGCGGCCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGAGCGCA
  GAGGATGTACAAGCAGTCGATGGCGCAGAGAGCGCGGACCATGGCCCTCTACAACCCCATCCCTGTCCGC
  CAGAACTGCCTCACGGTCAACCGCTCCCTGTTCCTCTTCAGTGAAGACAACGTGGATGACACGGAACCAT
  ACTTCATTGGAATCTTCTGTTTTGAGGCTGGAATTAAGATCGTGGCTCTTGGCTTTGCCTTCCACAAAGG
  CTCCTACCTGAGGAATGGCTGGAACGTCATGGACTTTGTCGTGGTGCTAACAGGCATCTTGGCCACTGTC
  GGGACGGAGTTTGACCTACGGACACTGAGGGCGGTTCGTGTGCTGCGGCCACTCAAGCTGGTGTCTGGAA
  TCCCAAGTTTACAAGTCGTCCTGAAGTCAATCATGAAGGCGATGATCCCTCTGCTGCAGATCGGCCTCCT
  CCTGTTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGGAAATTTCATACCACC
  TGCTTTGAAGAGGGGACAGACGACATCCAGGGTGAGTCGCCAGCTCCGTGTGGGACAGAGGAGCCTGCCC
  GCACCTGCCCCAACGGGACCAAATGTCAGCCGTACTGGGAAGGGCCCAACAACGGCATCACTCAGTTCGA
  CAACATCCTGTTTGCTGTGCTCACTGTTTTCCAGTGCATCACCATGGAAGGCTGGACTGATCTCCTCTAC
  AATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATCATCGGCTCCT
  TTTTTATGCTGAACCTTGTTCTGGGTGTGCTGTCTGGGGAGTTTGCCAAAGAAAGGGAACGTGTGGAGAA
  CCGGAGAGCTTTTCTGAAGCTCAGAAGACAACAGCAGATTGAACGTGAGCTCAATGGATACATGGAGTGG
  ATCTCAAAAGCAGAAGAGGTGATTCTTGCGGAGGACGAGACAGACGTGGAGCAGAGGCACCCTTTTGATG
  GAGCTCTTCGGAGAGCTACTCTGAAGAAAAGCAAGACAGACCTGCTCAACCCTGAGGAGGCCGAGGACCA
  GCTGGCTGACATCGCGTCTGTGGGGTCTCCCTTCGCCAGAGCCAGCATCAAAAGTGCCAAGCTGGAGAAC
  TCGACTTTTTTTCACAAGAAGGAGAGAAGAATGCGTTTCTACATCCGCCGCATGGTCAAAACTCAGGCCT
  TCTACTGGACCGTGCTCAGTCTGGTAGCCCTCAACACGCTGTGCGTCGCCATTGTCCACTACAACCAGCC
  CGAGTGGCTCTCCGACTTCCTCTACTATGCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTTT
  ATAAAAATGTACGGGCTCGGGACGCGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTCA
  TCATTGGGAGCATCTTTGAAGTCATCTGGGCTGTCATCAAACCTGGTACATCCTTTGGAATCAGTGTGTT
  ACGAGCTCTCAGGTTATTGCGAATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGAAACCTGGTTGTC
  TCCCTCCTCAACTCCATGAAGTCCATCATAAGTCTGTTGTTCCTCCTTTTCCTGTTCATCGTTGTCTTTG
  CCCTCCTGGGGATGCAGCTGTTCGGTGGCCAATTTAATTTTGATGAGGGGACTCCGCCCACCAACTTCGA
  CACTTTTCCAGCAGCAATAATGACTGTGTTTCAGATCCTGACTGGCGAGGATTGGAATGAGGTCATGTAT
  GATGGGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTACTTCATCGTCCTCACCC
  TCTTCGGGAACTACACCCTGCTGAACGTGTTCTTGGCCATCGCAGTGGACAACCTGGCCAATGCCCAGGA
  GCTCACCAAGGATGAGCAAGAAGAGGAAGAGGCAGCCAATCAGAAGCTGGCTCTACAGAAAGCCAAGGAG
  GTAGCAGAAGTGAGTCCCCTGTCTGCAGCCAACATGTCCATAGCCGTGAAGGAGCAGCAGAAGAACCAGA
  AGCCTACCAAGTCGGTGTGGGAGCAGCGCACCAGCGAGATGCGCAAGCAGAACCTGCTGGCCAGCCGCGA
  GGCGCTGTACGGGGACGCGGCTGAGCGCTGGCCCACCCCCTACGCGCGCCCGCTGCGGCCCGACGTGAAG
  ACTCACTTGGACCGGCCGCTTGTGGTGGACCCGCAGGAGAACCGTAACAACAACACCAACAAGAGCCGTG
  CGCCAGAAGCGCTGCGCCCGACCGCGCGGCCCCGCGAAAGCGCGCGCGACCCCGACGCGCGGCGCGCCTG
  GCCCGGCAGCCCTGAGCGCGCCCCTGGACGAGAGGGCCCGTATGGGCGCGAGAGCGAGCCTCAGCAGCGC
  GAGCACGCGCCGCCCCGCGAGCACGCACCCTGGGACGCGGACACCGAGCGCGCCAAAGCCGGGGACGCGC
  CCCGCCGCCACACGCACCGACCTGTGGCGGAGGGCGAGCCTCGCCGCCACCGCGCCCGCCGCCGGCCCGG
  GGACGAACCCGACGACAGGCCAGAGCGCAGGCCACGTCCCCGCGACGCCACTAGGCCGGCCCGCGCTGCA
  GACGGCGAAGGCGACGATGGGGAGCGCAAGCGGCGACACCGACACGGGCCGCCGGCCCACGACGACAGGG
  AGCGCAGACACCGACGCAGAAAAGAGAACCAGGGCTCTGGGGTCCCCGTGTCTGGTCCCAACTTGTCCAC
  CACCAGGCCAATCCAGCAGGACCTGGGCCGCCAGGACCTGCCGCTGGCTGAGGATCTTGACAACATGAAG
  AATAACAAGCTGGCCACCGGGGAACCTGCCAGTCCCCACGACAGCCTGGGCCACAGCGGCCTTCCCCCCA
  GCCCCGCCAAAATCGGGAACAGCACCAACCCTGGTCCCGCCTTGGCCACCAATCCCCAGAACGCTGCCAG
  CCGCAGGACGCCCAACAATCCGGGCAACCCGTCCAACCCCGGCCCCCCCAAGACTCCCGAGAACAGCCTT
  ATCGTCACCAACCCCAGCAGCACCCAGCCCAACTCAGCGAAGACTGCCAGGAAACCCGAGCACATGGCGG
  TGGAGATCCCCCCAGCCTGCCCGCCCCTCAACCACACCGTGGTCCAAGTAAACAAAAACGCCAACCCAGA
  CCCACTGCCAAAGAAAGAGGAAGAGAAGAAAGAGGAAGAGGAGGCAGACCCCGGGGAGGACGGCCCAAAG
  CCCATGCCGCCCTACAGCTCCATGTTCATCCTCTCCACCACCAACCCCCTTCGCCGGCTGTGCCATTACA
  TCCTGAACCTGCGCTACTTCGAGATGTGCATCCTCATGGTCATTGCCATGAGCAGCATCGCTCTGGCCGC
  CGAGGACCCGGTGCAGCCCAACGCACCCCGCAACAATGTGCTGCGATATTTTGACTATGTTTTTACAGGC
  GTGTTTACCTTTGAGATGGTGATCAAGATGATCGACCTGGGCCTCGTCTTGCATCAGGGGGCCTATTTCC
  GTGACCTATGGAACATTCTGGACTTCATAGTGGTCAGTGGGGCCCTGGTGGCCTTTGCCTTCACTGGCAA
  TAGCAAAGGAAAGGACATTAACACCATTAAGTCTCTCCGAGTCCTCCGGGTGCTACGACCTCTAAAGACC
  ATCAAGCGGCTGCCAAAGCTGAAGGCCGTGTTTGACTGCGTGGTGAACTCGCTCAAGAACGTCTTCAACA
  TCCTCATTGTGTACATGCTGTTCATGTTCATCTTCGCTGTGGTAGCCGTGCAGCTCTTCAAGGGCAAGTT
  CTTCCACTGCACGGACGAGTCCAAGGAGTTCGAGAGAGACTGTCGAGGCAAGTACCTCCTGTACGAGAAG
  AACGAAGTGAAGGCCCGGGACCGCGAGTGGAAGAAGTACGAGTTCCACTACGACAACGTGCTCTGGGCTC
  TGCTCACACTTTTCACAGTGTCCACAGGAGAGGGCTGGCCACAGGTCCTCAAGCACTCAGTGGATGCTAC
  TTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTA
  GTGTTTCCCTTCTTCTTTGTCAATATCTTCGTGGCCTTGATCATCATCACCTTTCAGGAGCAGGGAGACA
  AGATGATGGAAGAGTACAGCCTGGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGCC
  GCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCA
  CCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGTTCTACGGAG
  CCTCGGTGGCCTATGAAAACGCCCTTCGCGTGTTCAACATTGTCTTCACCTCCCTCTTCTCTCTCGAATG
  TGTGCTCAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGTG
  ACTGTTCTGGGCAGCATCACAGACATCCTCGTCACCGAGTTTGGGAATAACTTCATCAACCTGAGCTTTC
  TCCGCCTCTTCCGTGCTGCCAGACTCATCAAACTCCTCCGCCAGGGTTACACCATCCGCATTCTCCTCTG
  GACTTTCGTGCAGTCCTTCAAGGCCCTACCTTATGTCTGTCTGCTGATCGCCATGCTCTTCTTCATCTAC
  GCCATCATCGGGATGCAGGTGTTTGGCAACATCGGCATTGACGGGGAAGACGAGGACAGCGACGAGGATG
  AATTCCAAATCACGGAGCACAATAACTTCCGGACCTTCTTCCAAGCGCTCATGCTTCTCTTCCGGAGCGC
  CACAGGAGAAGCGTGGCACAACATCATGCTCTCCTGCCTCAGCGGGAAGCCGTGCGATAAGAACTCTGGG
  ATCCTCACGGCAGACTGCGGCAACGAGTTCGCGTACTTCTACTTTGTCTCGTTCATCTTCCTGTGCTCGT
  TTCTGATGCTGAATCTCTTTGTCGCTGTCATCATGGACAACTTCGAGTACCTCACCCGAGATTCTTCCAT
  CCTGGGCCCCCATCACTTGGATGAATACGTGCGTGTCTGGGCAGAGTATGACCCTGCTGCCTGCGGCCGC
  ATTCACTATAAGGACATGTACAGTTTATTGCGAGTAATATCGCCCCCTCTCGGCTTAGGCAAGAAATGTC
  CTCATAGGGTTGCTTGCAAGAGACTCCTGCGGATGGACCTGCCGGTAGCAGATGACAACACAGTTCACTT
  CAACTCCACCCTGATGGCTCTGATCCGAACCGCCCTTGATATCAAAATTGCCAAGGGTGGAGCTGACAAG
  CAGCAGATGGATGCAGAGCTTCGCAAGGAGATGATGGCCATTTGGCCCAACCTGTCTCAGAAGACCCTGG
  ATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGATGATCAT
  GGAGTATTACCGGCAGAGCAAGGCCAAGAAACTGCAGGCCATGCGAGAGGAGCAGAACCGGACACCACTC
  ATGTTCCAGCGCATGGAGCCTCCATCACCAACACAGGAGGGAGGACCCAGCCAGAATGCCCTTCCCTCCA
  CTCAGCTGGACCCAGGAGGAGGCCTGATGGCTCACGAAGGCGGCATGAAGGAGAGCCCGTCCTGGGTGAC
  CCAGCGGGCCCAAGAGATGTTCCAGAAGACTGGCACCTGGAGCCCAGAGCGAGGGCCACCCATCGACATG
  CCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGCGAGAAATGGGTACTGATGGCTACTCAGACAGCG
  AACACTACCTCCCCATGGAAGGCCAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAACCAGAG
  GAGAAGGGGCCGGCCACGTGGAAATGACCTCAGTACCATCTCTGATACCAGCCCCATGAAGCGCTCAGCC
  TCTGTGCTGGGACCCAAAGCCCGGCGACTGGATGACTACTCACTAGAGCGGGTACCGCCTGAGGAGAACC
  AACGGTACCACCAACGTCGCCGGGACCGTGGCCACCGCACCTCTGAGCGCTCTCTGGGCCGATACACTGA
  TGTGGACACAGGTTTGGGGACAGATCTGAGCATGACCACCCAATCGGGTGACCTGCCCTCCAAAGATCGG
  GACCAGGACCGGGGCCGACCCAAGGACCGGAAGCATCGGCCACACCACCACCACCACCACCATCATCATC
  ATCCCCCGGCCCCCGACCGGGATCGCTACGCACAGGAGCGGCCGGACACCGGCCGGGCGCGGGCCCGGGA
  GCAGCGCTGGTCCCGCTCGCCCAGCGAAGGTCGGGAGCACACGACACACAGACAGGGCAGTAGTTCCGTC
  AGTGGAAGCCCAGCCCCCTCTACGTCCGGCACCAGCACGCCGCGGCGCGGCCGCCGCCAGCTCCCCCAGA
  CCCCCTGCACCCCGCGGCCGCTTGTGTCCTACTCGCCCGCTCCGCGCAGGCCTGCGGCGCGCAGGATGGC
  GGGTCCCGCAGCGCCCCCTGGCGGCTCGCCGCGGGGTTGCCGCCGTGCGCCGCGCTGGCCTGCGCATGCG
  CCTGAGGGCCCGCGGCCGCGCGGTGCCGACTACACCGAGCCCGACAGCCCGCGCGAGCCGCCAGGGGGCG
  CGCACGATCCCGCGCCGCGGTCGCCCAGGACTCCGCGCGCCGCGGGCTGCGCGTCACCGCGCCACGGCCG
  CAGGCTGCCCAATGGTTACTACGCGGGGCACGGCGCGCCGCGGCCGCGCACAGCCCGCAGGGGCGCGCAC
  GACGCGTACAGCGAGAGCGAGGACGACTGGTGCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001252059
• Insert Size: 6966 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252059.1, NP_001238988.1
• RefSeq Size: 7788 bp
• RefSeq ORF: 6966 bp
• Locus ID: 12286
• Cytogenetics: 8 40.95 cM
• Gene Summary: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by the spider omega-agatoxin-IVA (AC P54282) (By similarity). They are however insensitive to dihydropyridines (DHP).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.