Wnk2 (NM_001290311) Mouse Untagged Clone

CAT#: MC229721

Wnk2 (untagged) - Mouse WNK lysine deficient protein kinase 2 (Wnk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnk2
• Synonyms: 1810073P09Rik; AW122246; ESTM15; mKIAA1760; X83337
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229721 representing NM_001290311
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGCTGATGGAAGCCGGGCGCGGCACGGGGTCGG
  CGGGCATGGCGGAGCCGCGGGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT
  GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCT
  CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGGCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG
  CCGCGCCCGCGCCCGCCGCGCCCGCTGCGCCTCCCGGCTCCCCGAGTGTCCCCTCGGACCCTGGCCCGGA
  GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCCTCAGCCGCAGCCGCGCAGGTCCCCGAC
  GGCGGCCCGAGGCAGGAAGAGGCGCCGGCCCCAACACAGGAGGACGCTGGAACCACCGAGGCAAAGCCGG
  AGCCCGGGCGCGCACGCAAGGATGAACCTGAAGAGGAGGAAGACGATGAGGACGACCTCAAGGCCGTGGC
  CACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC
  AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAGC
  TTGAACGGCAGAGATTCAAAGAGGAGGCGGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT
  CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAGCGATGTATCGTGCTGGTGACAGAGCTGATGACCTCG
  GGTACACTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCGAAGGTACTGAGGAGCTGGTGCCGGC
  AGATCCTGAAGGGCCTGCTGTTCCTGCATACAAGGACACCCCCCATCATCCACCGTGACCTCAAGTGTGA
  CAACATCTTCATCACTGGGCCCACCGGCTCTGTGAAGATTGGTGACTTGGGTCTGGCCACCCTTAAAAGA
  GCGTCTTTTGCCAAAAGTGTGATAGGTACCCCCGAGTTCATGGCACCTGAAATGTACGAGGAACATTACG
  ACGAGTCGGTTGATGTCTACGCCTTCGGGATGTGCATGCTGGAAATGGCTACCTCAGAGTACCCCTATTC
  GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAACCGGCCAGCTTCGAGAAG
  GTGCATGATCCGGAGATTAAGGAGATCATCGGGGAGTGCATCTGTAAGAACAAAGAGGAGAGGTATGAGA
  TTAAGGACCTGCTGAGCCATGCCTTCTTCGCGGAAGACACGGGTGTCCGGGTGGAACTTGCCGAGGAGGA
  CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTGGAAGACCCCAAGAAGCTGAAGGGCAAGCCC
  AAAGACAATGGAGCCATAGAGTTTACCTTTGACTTGGAGAAGGAGACTCCCGACGAGGTGGCCCAGGAAA
  TGATCGATTCTGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGTGACCGGGTGGC
  ACTGATTCAGTGGCGGCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACTCTGGCAGC
  CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCACGCACAGAGTGGGCAGC
  CTGGGCAGCCAGAACCTGAGGAGCCTGAGGCCGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT
  TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC
  AGCCAGCAGAGCATGGTGCTCAGCTCGCTTGTGGACACGGCCCCAACCCCAGCCTCGTGCGTGTGCAGCC
  CCCCTGTGAGTGAGGGGCCTGGCCTGACCCACAGCCTGCCTACGCTAGGGGCCTTCCAGCAGCCGGCCAC
  TGTGCCTGGCCTGTCTGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA
  GAATCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC
  CAGCACCCCCAGTCCAGCAGCCTCTTCCAATGGCCCAACCACCTACCCTGCCACAGGTCCTGGCCCCACA
  GCCCATGGGCACTGTCCAGCCAGTACCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTCGTG
  GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTGCAACCTCTTGCTCAAGTCCCTCCGC
  AGATGCCTCAGATGCCTGTGGTCCCCCCCATCACCCCCTTAACAGGACTCGATGGCCTCCCTCAGACCCT
  CACTGACCTGCCGGCCGCAAACGTGGCCCCCGTGCCACCACCTCAGTATTTCTCACCTGCTGTGATCTTG
  CCAAGCCTCACAACACCCCTGCCCACATCACCGGCCCTGCCTATGCAGGCTGTCAAGTTGCCTCATCCCC
  CTGGAACACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAATGCACCAGCTGCTATCCCCCTGTTGGC
  CGTGGCCCCGCAGGGTGTGGCTGCACTGTCTATCCATCCAGCGGTGGCCCAGATCCCAGCCCAGCCTGTG
  TACCCAGCAGCCTTTCCACAGATGGTACCTGGAGACATCCCACCCTCACCCCACCACACGGTGCAGAGCT
  TACGGGCCACCCCTCCACAGCTGGCCTCACCTGTGCCTCCTCAGCCTGTCCAGCCCAGTGTCATTCATCT
  ACCTGAGCAGGCTGCTCCAACTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCTCCTTCATACACC
  GCGGACGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTCCTCTGCCAGATA
  CTCTGCTGCCTACTGTCCCTGACCTCCTGCCCAAGGTCCCCAGCTCTCTGGCCCCTACAGTGGTCGCAGC
  CTCTCAGAGTGCGCCCGCACAGACCTCTTCACTCCTCCTGCCAACAAACCCACCCCTGCCCACTGGGCCT
  GCAGTTGCTGGCCCCTGCCCTGCAGTCCAGCTGATGGTGGAAGTGGCTCAAGAGGAGCAGGTCTCACAAG
  ACAAACCCCCTGGCCCCCCACAGAGCTCCGAGAGCTTTGGAGGTTCTGATGTCACCTCTGGAAGAGATCT
  GAGTGACAGCTGTGAAGGCACCTTTGGAGGGGGCAGGCTGGAGGGAAGGACCGCCCGGAAACACCACCGT
  AGGTCCACTCGTGCACGCTCGCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCCTAAATGTGTGTA
  ACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAAACGCATAACCATAAGATGGTGACCTTCAAGTTTGA
  CCTCGATGGGGACGCTCCGGATGAGATTGCCACGTACATGGTAGAGCATGACTTTATCCTGCCCGCTGAG
  CGAGAGACCTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAAGACATGCTCAGTGAGGACACAG
  ATGCTGACCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCTGGCCACGGGGGA
  GGAGAACAGACAGTCCCAAGCCAATGCCCCTGTCTACCAGCAGAATGTCTTACACACGGGAAAGAGGTGG
  TTTATCATCTGTCCAGTGGCTGAACACCCAGCAACAGACACTTCTGAATCATCACCCCCACTTCCTCTAA
  GCTCCTTGCAGCCAGAAGCCAGCCAAGACCCAGCGCCCTATCCAGACCAGCTGTCCTTGACGGACAAACC
  TAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGTGGAGCATCAGCC
  CCCTTGGCCCCGTCTTCCCCTCCTGTGACTACTGTAATCCCGGCAGCACCAGCCACCAGCACCGTGCCAG
  AGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGCCAGTGTCCATGA
  GACCCTCCAGCCACTGGCCGAGACACGCTCTGCCCAGTGTACTGCCCAGCCCCTTAGTACTGGCCAAGGA
  CCTTGCACCCCAGCTCTGGAAGCCTCTCGCTGCTCCACAGGTCTGGGGGAGCCCATCTCAACCAGGGAGG
  TCAGCACTCAAGGAGAGCCCCTGCCTGCTTCAGTGCCAGAACCTAGCCCTCCCACTGGGGCCACACAATC
  TGTACCGGGTCAGCCACCACCCCCACTGCCCATCACAGTAGGGGCTATCAGTCTGGCAGCTCCCCAGCTC
  CCCAGTCCCCCCCTGGGACCTACTGCTCCCCCACCACCCCCCTCAGCCTTGGAATCTGATGGGGAAGGGC
  CGCCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCGGACTCTGCTCTA
  CCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGGACCCCCATGGAGGCAAGCGATAGAGACTTCACC
  CTGGAGCCCCTGCGAGGAGACCTTCCCTCAGCCTTGAGTGACAAGACCCCAAGCCTCACCCAGCAGACCC
  AGCCCTCATTGGAAAAGTCTGAAACGGCCCCTGCAGGATGGGCCCTGGCCCAGAGGGAACAGGGTGCATC
  TTCTCCAATGACAGCAGAGTCATCTTCCAGCAATACACTGGGCTGTGACAGTGACGCAGGGCAGGTGGCC
  TCGGACTCCTCCACAGCACCCAGTGTCCCCCAGGATGCCTCTGGTTCTTCCGTCCCTACACACATGGATC
  CCAAAGATCAGAACAGTAGTGTTCCCAGAGAAGCCTTGGCAGCACCCATGCAAAGTGGCCCAGGGTCCTT
  CACAGTGGGCAGCCCAGCCCAGCTGCGTGGCGCCCGGGACTCCGGGTCTCCTCATAAGCGGCCAGGGCAG
  CAGGACAACAGTTCTCCAGCCAAAACTGTAGGGCGCTTTTCCGTGGTCAGCACACAGGACGAGTGGACTC
  TGGCGTCACCTCACAGCCTGCGGTACTCTGCCCCACCCGATGTCTACCTGGATGAGATCCCCTCTAGCCC
  TGAGGTGAAGCTGGCAGTGCGGCGGGTACAGACAGCCTCCTCCATCGAGGTTGGCGTTGAGGAACCTGCC
  TCTAGTGACTCTGGGGATGAACGCCCAAGAAGGAGGTCCCAGGTGCAGAAGCAGTCCTCCCTGCCGGGTA
  CTGGTGGTGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCTTCAAGGGCTGGCTC
  GCTGGGACCTGAGACACCCAGTAGGGCAGGTGTGAAGGTCCCTACCATCAGCATCACCTCCTTCCACTCC
  CAGTCATCCTACATCAGCAGTGACAATGACTCAGAGTTTGAGGATGCAGACATAAAGAAGGAACTGCGCA
  GTCTGCGAGAAAAGCACCTGAAGGAAATCTCGGAGCTACAGAGCCAGCAGAAGCAGGAGATCGAAGCCCT
  GTACCGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACTGCACCCCCTATGGGCCGC
  AGGAGGAAAACCAGCAAGAGCAAATTGAAGGCTGGCAAGCTGCTGAACCCCTTGGTGCAACAGCTCAAGG
  TCGTGGCCTCCAGCACAGGTCATTTGTCTGACTCCAGCAGAGGCCCTCCCACTAAGGACCCTGCCCACGC
  CAGCACGCCCCCGGGCACCAAGGCAGTTCAGACCCAGCAGCCCTGCTCCGTTCGGGCCTCTCTGTCCACA
  GACATCTGCTCCGGTTTAGCCAGTGATGGAGGCGGAGCGCGTGGCCAAGGCTGGACGGTTTACCACCCAA
  CGTCTGAGAGAGGGGCCTATAAGTCTAGTAGCAAGCCTCGTGCTCGATTCCTCAGTGGACCCGTATCTGT
  GTCCATCTGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAGCAGTAGGTCTTCCACCAGCAGC
  CTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAGGCGCAGGTGAACAACAGCAACA
  ACAAGAAAGGCACCTTCACGGACGACCTGCACAAGTTGGTGGACGAGTGGACAACGAAGACCGTGGGGGC
  AGCGCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCTGCACGACATGGAGGCCTCTGGA
  GACGCTCGGGCTACATCTGTGCCTCGAGCAGCAGTGGGGGCATCGTGTCTGGCCCCGGCCCCTGGCCCTC
  TGTCCACCACAGCCACTCCTGGAGCCACTCCAGCCCTGCCTGTGCCCATACCAGGTACTGTCCACTCCAG
  CCTCTCAGGCCCTCCTTGTACACTGCCTCTATGTCAGTATGGAGGCCTGCTCCCTGATCCTGTGTCCTGG
  GGACCCTGGGTGGCCTCAGGAAACCCAGAAGGGTCTTGGGGCAGTCAGAGCCCCACTCAGGTCCCTGTGT
  TCCCAGTGTTTCTACGGCCACCTGTTATCAGCACACCAGGCCCCCGGCTGCACATCACGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290311
• Insert Size: 6642 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290311.1, NP_001277240.1
• RefSeq Size: 8496 bp
• RefSeq ORF: 6642 bp
• Locus ID: 75607
• UniProt ID: Q3UH66
• Cytogenetics: 13 25.07 cM
• Gene Summary: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). This variant is partially supported by the mRNA AK044381.1; the 5' exon structure is inferred based on other transcript alignments at this gene. CCDS Note: This CCDS representation is partially supported by the mRNA AK044381.1. The 5' exon structure is inferred based on other transcript alignments at this gene.