Cacna1c (NM_001255998) Mouse Untagged Clone

CAT#: MC229720

Cacna1c (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1c
• Synonyms: Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229720 representing NM_001255998
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGGGCCTTTGTTCAGCCATCCACACCCCCATACCAGCCACTCTCCAGTCACTCGTCTGAGGAAA
  CAGAGAGGAAGTTCAAGGGCAAGGTGGTACATGAAGCTCAACTCAACTGTTTCTACATTTCTCCTGGAGG
  TTCCAACTATGGGAGCCCACGCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCC
  GAGCACATCCCTACTCCAGGGGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGC
  TCATGGGCAGTGCTGGCAACGCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGG
  GAAACCCAAGAAGCAGGGGGGCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAG
  AACCCTATCCGGAGGGCGTGCATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTA
  TTTTTGCCAATTGTGTGGCCTTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTC
  CAACCTGGAACGAGTGGAGTATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCC
  TACGGACTTCTCTTCCACCCCAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGG
  TTGTAGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAA
  AGGAGCTGGATTCGACGTGAAGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGA
  GTCCCAAGTCTCCAGGTGGTCCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTC
  TTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGAC
  CTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACA
  GGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCA
  CCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGA
  CGTGCTGTACTGGATGCAAGACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATC
  TTTGGATCCTTTTTCGTTCTAAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGA
  AAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTA
  CCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAG
  CCTCGAAACAGAGGCGCTCCAGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACT
  CTACAGAAACCCATGTGAGCATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGG
  TGACATCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTAC
  TGGCGCAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGC
  TCGTGATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCT
  CACAGAAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATG
  TACAGCCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGG
  GCATCCTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGT
  GCGGTTGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTG
  AACTCAGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGG
  GGATGCAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTT
  CCCGCAGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGG
  ATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCT
  GTGGAAATTATATCCTACTGAATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCT
  GACCTCAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAA
  AAGAAACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCA
  TTACAGCCGATGGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGA
  GGATAAGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTG
  GGGCCACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTG
  CATTTTTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTT
  CACCAACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCAC
  ACCTCCTTCAGGAACCATATTCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTG
  CTCTAAAGATGACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCT
  GGACCTGCTGGTGGTTAGCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAG
  ATTTTACGAGTGCTGCGAGTCCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGG
  TTCAGTGCGTGTTTGTGGCCATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCAT
  GTTCGCCTGCATTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACG
  GAGGCAGAATGCAAGGGTAACTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTC
  GAAGCTGGGAGAACAGCAAGTTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTC
  CACCTTCGAAGGGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATC
  TACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGA
  ACATCTTCGTGGGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCT
  GGACAAGAACCAGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAG
  AACCAGCACCAGTACAAAGTGTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCA
  TCCTGCTCAACACCATCTGCCTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAA
  TATACTCAACATGCTTTTCACCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCC
  AAGGGTTACTTTAGTGATCCCTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCA
  TTCTCAGTGAGACTAATCCAGCTGAACATACCCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCG
  CATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGC
  ATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGA
  TGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGA
  GATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGA
  GAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCA
  ACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCT
  CTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTAGGGAT
  TGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCA
  AGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAA
  ATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACA
  GTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAG
  AGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGA
  CCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAA
  GAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCAC
  TGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGA
  TCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATC
  TTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTC
  CTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTC
  ACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCC
  AACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCA
  GCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAG
  CTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACC
  CGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCT
  CCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCC
  AAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGA
  CAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCAT
  TGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCC
  CACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGG
  GCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGA
  CAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGC
  TGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGA
  CTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACA
  TGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGG
  CACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGC
  TGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACC
  TGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001255998
• Insert Size: 6585 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255998.2, NP_001242927.1
• RefSeq Size: 13415 bp
• RefSeq ORF: 6585 bp
• Locus ID: 12288
• Cytogenetics: 6 55.86 cM
• Gene Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 6, which is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.