Myo7a (NM_001256083) Mouse Untagged Clone

CAT#: MC229711

Myo7a (untagged) - Mouse myosin VIIA (Myo7a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo7a
• Synonyms: Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229711 representing NM_001256083
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGAAGTCAGGCCAGGAGTTTGATGTGCCCATCGGGGCCGTGGTGAAGCTCTGCGACTCGGGCC
  AGATCCAGGTGGTGGATGATGAAGACAATGAACACTGGATATCCCCTCAGAATGCCACGCACATCAAGCC
  AATGCACCCCACATCGGTGCACGGCGTGGAGGACATGATCCGCCTGGGGGATCTCAACGAGGCAGGCATC
  CTTCGAAACCTTCTCATTCGCTACCGGGACCACCTCATCTATACGTACACAGGTTCCATCCTGGTGGCCG
  TGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTACACCAACAAGAAGATAGG
  GGAGATGCCCCCCCACATCTTCGCCATTGCTGACAACTGCTACTTCAACATGAAACGCAACAACCGGGAC
  CAGTGCTGTATTATCAGCGGGGAGTCGGGAGCTGGCAAGACAGAGAGCACAAAGTTGATCCTGCAGTTCC
  TGGCAGCCATCAGTGGACAGCACTCATGGATCGAGCAGCAGGTGCTGGAGGCCACCCCGATCCTGGAAGC
  ATTTGGGAACGCCAAGACCATCCGCAACGACAACTCTAGCCGCTTTGGCAAGTACATTGACATCCACTTT
  AACAAGCGTGGTGCCATCGAGGGCGCCAAAATAGAGCAATACCTGCTGGAGAAGTCACGTGTCTGCCGCC
  AGGCCCCTGACGAGAGGAACTATCACGTGTTCTACTGTATGCTGGAGGGCATGAATGAGGAGGAGAAGAA
  GAAACTGGGCCTAGGCCAGGCCGCTGACTACAACTACTTGGCCATGGGTAACTGCATCACCTGTGAGGGC
  CGCGTGGACAGTCAGGAGTATGCCAACATCCGCTCTGCCATGAAGGTTCTCATGTTCACAGACACGGAGA
  ACTGGGAGATCTCGAAGCTTCTGGCTGCCATCCTACACATGGGCAATCTGCAGTATGAGGCCCGGACATT
  TGAGAACTTGGATGCGTGTGAAGTCCTCTTCTCCCCATCGCTGGCCACGGCAGCTTCTCTGCTCGAGGTG
  AACCCCCCAGACCTGATGAGCTGCCTCACCAGCCGCACCCTCATCACCCGTGGGGAGACGGTGTCCACCC
  CTCTCAGCAGGGAACAGGCGCTGGATGTGCGAGATGCCTTTGTCAAGGGCATCTATGGGCGGCTCTTTGT
  GTGGATTGTGGAGAAGATCAACGCAGCAATCTACAAGCCACCCCCCCTGGAAGTGAAGAACTCTCGCCGG
  TCCATCGGTCTCCTGGACATCTTTGGATTTGAGAACTTCACTGTGAACAGCTTCGAGCAGCTCTGCATTA
  ACTTTGCCAATGAGCACCTGCAGCAATTCTTCGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAGTACGA
  CCTGGAGAGCATCGACTGGTTGCACATTGAGTTCACTGACAACCAGGAAGCACTGGACATGATTGCCAAC
  CGGCCTATGAACGTCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACGGATGCCACCATGC
  TGCATAAGCTGAACTCACAGCACAAGCTCAATGCCAACTACGTGCCACCCAAGAACAGCCACGAGACCCA
  GTTTGGAATCAACCACTTTGCGGGTGTTGTCTATTATGAGAGTCAAGGCTTCCTGGAGAAGAACCGAGAC
  ACCCTGCATGGGGACATCATCCAGCTGGTCCACTCTTCCCGGAACAAGTTCATAAAGCAGATTTTCCAAG
  CTGACGTTGCCATGGGTGCCGAGACCAGGAAGCGCTCGCCTACACTCAGCAGCCAGTTCAAGCGGTCTCT
  GGAGCTGCTGATGCGCACACTGGGCGCCTGCCAGCCCTTCTTTGTGCGTTGTATCAAACCCAATGAGTTC
  AAGAAGCCCATGCTCTTCGACCGGCACTTGTGTGTACGCCAGCTGCGATATTCGGGCATGATGGAGACAA
  TCCGCATCCGCCACGCAGGCTACCCCATTCGCTACAGCTTTGTGGAGTTTGTGGAGCGCTACCGGGTACT
  GCTGCCTGGTGTGAAGCCAGCATACAAGCAGGGTGACCTCCGAGGGACATGCCAGCGCATGGCTGAGGCT
  GTGCTGGGCACGCACGATGACTGGCAGATTGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACATGT
  TGCTGGAGGTGGAGCGGGACAAGGCCATCACAGACAGAGTCATTCTCCTCCAGAAGGTTATCCGGGGCTT
  CAAAGACAGGTCCAACTTCCTGAGACTGAAGAGTGCTGCCACACTGATCCAGAGGCACTGGCGGGGCCAC
  CACTGTAGGAAAAACTATGAGCTGATTCGTCTTGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGA
  AGCTGCACAAGCAGTACCGCCTGGCCAGACAGCGCATAATAGAGTTCCAGGCCCGCTGCCGGGCCTATCT
  GGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCCGTGATCACCGTGCAGGCCTATGCCCGAGGCATGATT
  GCCCGCCGGCTACACCGCCGCCTCCGGGTTGAGTACCAGCGGCGCCTCGAGGCAGAGAGGATGCGTCTGG
  CAGAGGAGGAGAAACTCCGAAAGGAGATGAGTGCCAAGAAGGCCAAAGAGGAGGCTGAGCGCAAGCATCA
  GGAGCGCCTGGCTCAGCTAGCCCGCGAGGATGCGGAGCGGGAACTGAAGGAGAAGGAGGAGGCTCGGAGG
  AAGAAGGAACTGCTGGAGCAGATGGAGAAGGCCCGCCACGAACCCATCAACCACTCAGATATGGTGGACA
  AGATGTTTGGCTTCCTGGGGACTTCAGGCAGCCTGCCAGGCCAGGAAGGCCAGGCGCCTAGTGGCTTTGA
  GGACCTAGAGCGCGGACGGAGGGAGATGGTGGAAGAGGATGTTGACGCTGCCCTGCCCCTGCCTGATGAA
  GACGAGGAGGACCTTTCTGAGTACAAATTCGCCAAGTTTGCTGCCACCTACTTCCAGGGCACAACCACAC
  ACTCCTACACCCGGAGGCCTCTCAAGCAGCCGCTGCTCTACCACGACGATGAGGGTGACCAGCTGGCGGC
  GCTGGCTGTCTGGATCACCATCCTCCGGTTCATGGGGGACCTCCCAGAGCCCAAGTACCACACAGCCATG
  AGCGACGGCAGTGAGAAGATCCCAGTGATGACTAAGATCTACGAGACCCTAGGCAAGAAGACATATAAGA
  GGGAGCTGCAGGCCTTGCAGGGCGAGGGCGAGACCCAGCTCCCTGAGGGGCAGAAGAAGACCAGTGTGAG
  ACACAAGTTGGTACACTTGACACTGAAGAAAAAGTCCAAACTCACAGAAGAGGTGACCAAGAGGCTGAAC
  GATGGGGAATCCACGGTACAGGGCAACAGCATGCTGGAGGATCGGCCCACCTCAAATCTAGAGAAGCTGC
  ACTTCATCATCGGCAACGGCATCCTGCGGCCTGCGCTGCGGGACGAGATTTACTGCCAGATCAGTAAGCA
  GCTCACACACAACCCATCCAAGAGCAGCTATGCCAGGGGCTGGATCCTCGTGTCGCTCTGTGTGGGCTGC
  TTCGCCCCCTCTGAGAAGTTCGTTAAGTACCTGCGGAACTTCATCCACGGAGGCCCACCTGGCTATGCTC
  CTTACTGTGAGGAGCGCCTGAGGAGGACCTTTGTCAACGGAACTCGGACACAGCCACCCAGCTGGCTGGA
  GCTGCAGGCCACCAAGTCCAAGAAGCCCATCATGTTGCCCGTGACCTTCATGGATGGGACCACCAAGACC
  CTGCTAACAGATTCAGCAACTACAGCCAGGGAGCTGTGCAATGCTCTGGCTGACAAGATCTCACTCAAGG
  ACCGCTTTGGCTTCTCCCTCTACATCGCTCTGTTCGATAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCA
  TGTCATGGATGCCATCTCTCAGTGTGAGCAGTACGCCAAGGAGCAGGGTGCTCAGGAGCGCAACGCCCCA
  TGGAGGCTCTTCTTTAGAAAGGAGGTCTTCACACCCTGGCACAACCCCTCGGAGGACAACGTGGCCACGA
  ACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCT
  GGCTGAGCTGGCTTCTCAGCAGTACTTTGTGGACTATGGTTCTGAGATGATTCTGGAGCGCCTGCTGAGC
  CTCGTGCCCACTTACATCCCTGACCGTGAGATCACACCGCTGAAGAATCTTGAGAAGTGGGCACAGCTGG
  CCATTGCTGCCCACAAGAAGGGAATTTATGCCCAGAGGAGAACTGACTCCCAGAAGGTCAAAGAGGATGT
  GGTCAATTATGCCCGTTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTACGAAGCTTACAAATTCTCAGGC
  CCTCCCCTCCCCAAGAGCGACGTCATCGTGGCTGTCAACTGGACGGGTGTGTACTTCGTGGACGAGCAGG
  AGCAGGTGCTTCTGGAGCTGTCCTTCCCGGAGATCATGGCTGTGTCCAGCAGTAGGGGAACAAAGATGAT
  GGCCCCCAGCTTTACCCTGGCCACCATCAAAGGAGATGAGTACACCTTCACATCCAGCAATGCTGAGGAC
  ATCCGTGACCTGGTGGTCACCTTTCTGGAGGGGCTACGGAAGAGGTCTAAGTATGTGGTGGCACTGCAGG
  ACAATCCTAACCCTGCTGGTGAGGAGTCAGGCTTCCTCAGCTTCGCCAAGGGAGACCTCATCATCCTTGA
  CCATGATACTGGTGAGCAGGTCATGAACTCAGGCTGGGCCAACGGCATCAACGAGAGGACCAAGCAGCGC
  GGCGACTTCCCCACTGACTGTGTATACGTCATGCCCACTGTCACCTTGCCACCAAGGGAGATTGTGGCCC
  TGGTCACTATGACCCCAGACCAGAGGCAGGATGTCGTCCGGCTCCTGCAGCTTCGCACAGCAGAGCCAGA
  GGTGCGCGCCAAGCCCTACACGCTAGAGGAGTTCTCCTACGACTACTTCAGGCCCCCACCCAAGCACACG
  CTGAGCCGTGTCATGGTGTCCAAGGCCCGCGGTAAGGACAGGCTGTGGAGCCACACACGAGAGCCCCTCA
  AGCAGGCCCTGCTCAAGAAGATCCTGGGCAGTGAAGAACTCTCCCAGGAAGCCTGCATGGCCTTTGTAGC
  TGTGCTCAAGTACATGGGCGACTACCCATCCAAGAGGATGCGATCCGTCAATGAGCTCACTGACCAGATC
  TTTGAGTGGGCACTCAAGGCTGAGCCCCTCAAGGATGAGGCCTACGTGCAGATCCTGAAGCAGCTGACTG
  ACAATCACATCAGGTACAGCGAAGAGAGGGGCTGGGAACTGCTGTGGCTGTGCACGGGCCTCTTCCCGCC
  CAGCAACATCCTCCTGCCTCATGTTCAGCGGTTTCTGCAGTCCCGCAAGCACTGTCCTCTTGCCATTGAC
  TGCCTGCAGAGGCTCCAGAAAGCCCTGAGAAATGGCTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGG
  AGGCCATCCAACATAAGACTACCCAGATCTTCCACAAGGTCTACTTCCCCGATGACACGGACGAGGCTTT
  TGAGGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCAGCCGGCTGCTGCTCAAGTCT
  TCCGAGGGATTCAGCCTTTTTGTCAAAATCGCAGATAAGGTCATCAGCGTCCCAGAGAATGATTTCTTCT
  TTGACTTTGTCCGACACCTGACAGACTGGATAAAGAAAGCACGGCCCATCAAGGACGGAATCGTGCCCTC
  ACTAACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACAGTGCCGGGCAAGGACCCCATGGCT
  GACTCCATCTTCCACTATTACCAGGAACTGCCCAAATATCTCCGAGGCTACCACAAGTGCACCCGGGAGG
  AGGTGCTGCAGCTGGGCGCACTCATCTACAGGGTCAAGTTTGAGGAGGACAAATCCTACTTCCCTAGCAT
  CCCCAAGTTGCTGAGGGAGCTGGTACCCCAGGACCTAATCCGGCAGGTCTCACCTGATGACTGGAAACGG
  TCTATTGTCGCCTACTTCAACAAACATGCGGGGAAGTCCAAGGAGGAAGCCAAGCTGGCCTTCCTCAAAC
  TCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTTGAGGTGAAGCAAACTACAGAACCAAACTTCCC
  AGAGATTCTCTTAATTGCCATCAACAAGTACGGGGTCAGCCTCATCGATCCCAGAACCAAGGACATCCTG
  ACTACTCACCCCTTCACCAAGATCTCCAACTGGAGTAGTGGCAACACCTACTTCCACATCACCATTGGGA
  ACTTGGTCCGTGGGAGCAAACTGCTCTGTGAGACATCGCTGGGATACAAAATGGATGATCTTCTGACTTC
  CTACATCAGCCAGATGCTCACAGCCATGAGCAAGCAGAGGAACTCCAGGAGTGGAAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256083
• Insert Size: 6501 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256083.1, NP_001243012.1
• RefSeq Size: 7196 bp
• RefSeq ORF: 6501 bp
• Locus ID: 17921
• UniProt ID: P97479
• Cytogenetics: 7 53.57 cM
• Gene Summary: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.