Cacna1c (NM_001256001) Mouse Untagged Clone

CAT#: MC229708

Cacna1c (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 9

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1c
• Synonyms: Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229708 representing NM_001256001
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC
  GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG
  GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC
  GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG
  GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG
  CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC
  TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT
  ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC
  CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA
  ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA
  AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT
  CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC
  ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA
  TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA
  GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC
  TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATG
  ATGCCGTAGGAAGGGACTGGCCCTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACT
  TAACTTGGTTCTCGGTGTTCTTAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT
  TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG
  CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCC
  AGCGGGCTTGCATGATCAGAAGAAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGC
  ATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACT
  GTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATT
  CTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTC
  CTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAG
  CCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGC
  CTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTG
  GTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCA
  AGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGC
  CTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGG
  AAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTG
  TGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCC
  CTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTG
  AATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGG
  AGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGCCGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGA
  GGTGATGGAGAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGAT
  GGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTC
  CCCACTCCAACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCC
  CCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATC
  TTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCA
  TCCTCTTCTTCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAG
  GAACCATATTCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTAAAGATG
  ACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGG
  TGGTTAGCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGT
  GCTGCGAGTCCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTG
  TTTGTGGCCATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCA
  TTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATG
  CAAGGGTAACTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAG
  AACAGCAAGTTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAG
  GGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCG
  TGTGGAGATCTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTG
  GGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACC
  AGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCA
  GTACAAAGTGTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAAC
  ACCATCTGCCTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACA
  TGCTTTTCACCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTT
  TAGTGATCCCTGGAATGTTTTTGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAG
  ACTAATAGTGCAGAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGG
  TGAAGCTGCTGAGCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCT
  GCCCTATGTGGCTCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGG
  AAGATTGCCCTGAATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGT
  TACTGCTGTTCAGGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAA
  GTGTGCCCCAGAGTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTC
  TTCTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGG
  ACAACTTTGACTACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAAT
  CTGGGCCGAGTATGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGA
  ATTCAGCCCCCACTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGA
  ACATGCCTCTGAACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCT
  GAGGATCAAAACAGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGG
  AAGAGGACTAGCATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCA
  AGTTCTATGCCACCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGT
  GGGCAAGCCCTCACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCT
  GAGATCCGGCGGGCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGG
  TGTCTGCTGCCTCCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTA
  TCAGAGTGACAGCAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACA
  GGGAACAACCAAGCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCA
  GCTACTCATCCACGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCA
  TCCCGCTGGCTACTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGA
  GTACAGGAGGCAGCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGA
  ATCAGGAGATATTTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCC
  CAGCCTGCTCTCCACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAG
  GACAAGAGGGAGATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCT
  CCTTCCATCTGGAATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCC
  CTTGCATCTGGTTCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCT
  CCTACCACATTCCCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGC
  CCATCCCTACCCTACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCA
  CTGCAGCTCCTGGTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCC
  GTCTCCCTCACCGTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGG
  TGGAAGCGGTCTTGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCAC
  CCAGGAGCTGGCTGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGT
  GGGGGCGCCCAGCAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACA
  GGGCTGTGGCCCCAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGC
  GGACAGCAGGTCCTACGTCAGCAACCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256001
• Insert Size: 6471 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256001.2, NP_001242930.1
• RefSeq Size: 13643 bp
• RefSeq ORF: 6471 bp
• Locus ID: 12288
• Cytogenetics: 6 55.86 cM
• Gene Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (9) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (9) that is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.