Myo18a (NM_001291212) Mouse Untagged Clone

CAT#: MC229699

Myo18a (untagged) - Mouse myosin XVIIIA (Myo18a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo18a
• Synonyms: MAJN; MyoPDZ; MysPDZ; SP-R210
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229699 representing NM_001291212
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACCTCATGAAGAAAGATAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAAAAAAAGGAGA
  AGAAGGAGAGGATGTCTGCGGCAGAACTGCGGAGCCTGGAAGAGATGAGCATGCGCCGTGGCTTTTTCAA
  CTTGAACCGGTCCTCCAAGCGGGAATCTAAGACGCGCCTGGAAATCTCCAACCCCATCCCTATCAAGGTG
  GCCAGTGGCTCTGACCTGCACCTGACGGACATTGACTCTGACAGCAATCGGGGTAGCATCATCCTGGACT
  CAGGACACCTAAGCACAGCCAGCTCCAGTGATGACCTCAAGGGTGAAGAAGGCAGCTTCCGGGGCTCTGT
  GCTGCAGCGGGCAGCCAAGTTCGGTTCACTGGCCAAGCAGAACTCTCAGATGATTGTCAAACGTTTTTCC
  TTCTCTCAACGGAGCCGGGATGAGAGTGCCTCGGAGACCTCAACCCCCTCAGAGCACTCTGCAGCCCCAT
  CACCTCAGGTAGAGGTGCGGACGCTTGAGGGACAGCTAATGCAGCATCCTGGGCTAGGCATTCCTCGACC
  AGGACCACGGTCTCGAGTCCCTGAGCTGGTGACTAAAAGATTCCCAGCTGATCTACGTCTTCCCGCTCTG
  GTGCCACCACCACCTCCTGCTCTCCGGGAGCTGGAGTTACAACGAAGACCCACAGGAGACTTTGGCTTCT
  CACTGCGGCGCACAACCATGCTGGATCGGGCCCCTGAGGGTCAGGCTTATCGACGGGTGGTTCACTTTGC
  TGAGCCAGGGGCAGGCACCAAGGACCTGGCCTTGGGGCTGGTACCAGGAGATCGACTGGTGGAGATTAAT
  GGACAGAATGTGGAGAATAAATCCAGGGATGAAATTGTGGAGATGATCCGGCAGTCTGGGGACAGTGTGC
  GGCTCAAGGTACAGCCCATCCCAGAGCTTAGCGAGCTGAGCCGGAGCTGGCTTCGGACTGGCGAGGGACA
  CCGCAGGGAGCCGGCTGATCTGGACCCCGAGGCCGCCTCTCCTGCCTACAGCCAGGCCAAGACGGAAGAG
  CAGATTGCCGCTGAAGAGGCCTGGTATGAGACGGAGAAGGTGTGGCTGGTCCATAGAGATGGATTCTCCT
  TAGCCAGCCAGCTCAAGTCTGAGGAGCTCAGCCTACCCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGA
  CGGAGCCATCCTGGATGTGGATGAAGATGATATAGAGAAGGCTAATGCTCCTTCATGTGACCGTCTAGAA
  GATCTGGCCTCACTGGTATACCTCAACGAGTCTAGCGTCCTGCATACACTGCGCCAGCGCTATGGGGCTA
  GCCTGCTACACACCTATGCTGGCCCCAGCCTGCTTGTCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTC
  AGAGAAGGTGATGCACATGTTCAAGGGGTGTCGGCGGGAGGACATGGCACCCCACATCTACGCTGTAGCC
  CAGACTGCATATAGGGCGATGTTGATGAGTCGTCAGGACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCA
  GTGGCAAGACTACCAGCTTTCAGCATCTGGTGCAGTATCTGGCTACCATCGCGGGTACCAGTGGGACCAA
  GGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGTCCACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATC
  ATGAATGGCAGTGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTT
  CGGCCTCCATCCAGACAATGCTCCTGGAGAAGCTGCGTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTT
  CAATGTCTTCTACTACTTGCTGGCCTGTGGGGATGCCACCCTCAGGACAGAGCTCCACCTGAACCACTTG
  GCAGAGAACAATGTGTTTGGAATTGTGCCACTGTCCAAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGT
  TCAGTAAGCTCCAGGCTGCCATGAAGGTGCTGGCCATCTCCCCCGAGGAACAGAAGACCTGCTGGCTCAT
  CCTGGCTTCCATCTACCACCTGGGGGCTGCTGGAGCTACCAAAGAAGCTGCTGAAGCTGGACGAAAGCAG
  TTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATACCTGCTGGGCTGCAGCTTGGAAGAGCTGTCCTCGG
  CTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGCAACGGTCCACCTCCTTCCGTCAGGGCCCTGAGGA
  GAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCCTCTAC
  AGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAACAGGGCTCTCAAGTCCAGCCAGCACTCACTCTGTT
  CCATGATGATCGTGGACACACCAGGCTTCCAGAACCCTGAATGGGGCGGCTCGGCCCGTGGAGCCTCCTT
  CGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCTGCAGAGGCTGTTCCACGAGCGCACCTTCCTCCAG
  GAGTTGGAGAGATACAAGGAGGATAACATAGAACTGGCGTTTGATGACTTGGAGCCAGTTGCAGATGACT
  CCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTCGCTCACTGGCCCATGCCGATGAGGCAAGAGGGCT
  ACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGGTGCGACAGAGGATGCCCTCCTTGATCGTCTTTTC
  TCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGCCAGAGTCCTCTTCTGCGAAGTAGCAAACCTCGGC
  ACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGGTGGAGTACAACGTGGCTGGCTGGCTGAATTACAC
  CAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCTTCTGCAGGACTCCCAGAAAAAGATCATCAGTAAC
  CTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTCTCAGGCTCGATTGCAGGCCTGGAAGGTGGCTCCC
  AGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAACCTTCACCACCGGCATGGCCGCTGTCAAGAAGAA
  GTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGCCCTCATTGATACCATCAAGAGGTCCAAGATGCAT
  TTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGGCCTGGGGAGCCCCGGTCTGCCTCTTCCCGCAGAG
  TCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAGACCCCTGTGAGGCTGGGCTGCTACAACTGGATGT
  GTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCTGCTTGATGCGATGCGCATGTACCGCCAAGGTTAC
  CCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGCTTTGATGTCCTGGCTCCACACCTGACCAAGAAAC
  ATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGGCTGTGGAAGAGCTGCTGGAGTCCCTGGACCTGGA
  AAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTTCTTCCGGGCAGGCACGTTGGCACGGTTGGAAGAA
  CAACGAGACGAGCAAACCAGCAGGCACCTGACCCTGTTCCAGGCAGCCTGCCGGGGCTACCTTGCCCGCC
  AGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAAGAAGAACAA
  GGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCACCACCGTGCGGCCCCTCATCCAAGTTCAGCTGTCA
  GAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAGCAGCTGCGCAGCAAGCTTGAGAAGGTGGAGAAGG
  AGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAGAGACCCGGATCTCAGAACTGACATCGGAGCTGAC
  TGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGGCTCCGGACC
  GAGAAGGAGATGAAAGAGCTACAGACCCAGTATGACGCACTGAAGAAGCAGATGGAGGTGATGGAGATGG
  AAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGATCAACGGGGAGGTGGATGATGATGATGCAGGGGG
  AGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTCCAGCAGGAG
  CTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCTGCAAG
  CAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGCTCAAGAAGAAGTGCCAAAGGCTCACAGCTGAGCT
  GCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGAAACCACGAGCTGGAGAAGAAGCAGAGGAGG
  TTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACGCAGCGGGAGAAGCTGCAGAGGGAGAAACTCCAGC
  GGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCTTGAAGCAGCAAATGGAGGAAAAAGACTTGGACAT
  TGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGCTGAGCTTCAGGACATTTCTTCTCAAGAGTCTAAG
  GATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGGACCAGGAAG
  AGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATGGAGAT
  GGAGAGAATGAGACAGACCCATTCCAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAAGAGGCCCGGCAG
  TCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAGCTCGAGGAGGAGTATGAAGACAAGCAGAAGGCGC
  TGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCGGGACTTTGA
  ATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCCCAGATCATGCTG
  GACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGGAAGAGTCGG
  AATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAGCGATGGAGGTGGAGATGGAAGACCTGCACCTGCA
  GATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAACTGAGTCGACTTCAGCGTGAGAAAAAC
  GAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGACATGAATGAGCTGATGAAGAAGCACAAGGCAGCTG
  TGGCCCAGGCCTCCCGGGACATGGCACAGATGAACGATCTCCAGGCTCAGATTGAAGAGTCCAACAAGGA
  GAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTG
  GACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGTTCGAAAAGA
  CCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGCTCAAAGAAACCATGGAGAAGCTGACTGAGGAACG
  GGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAGCTTCGTGAC
  ACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAAGCCGAGGCTAGCCGCAAGAAGCATGAACTGGAGA
  TGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCCTGCAAGCCGACCTAAAGCTGGCGTTCAAACGCAT
  TGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGAAAGTGACGAGAACGAGGACCTCATCAACAGTGAG
  GGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGGGTCGACGGGGTCAAGTCCTGGTTGTCAAAGAACA
  AGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCTTGAAGAGTTCCAGCCCAACCAGCCACTGGAAGCC
  ACTCGCCCCTGACCCATCGGATGATGAGCATGATCCTGTGGACAGCATCTCCAGACCCCGGTTCTCCCAC
  AGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACAGAGACCAGTGCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291212
• Insert Size: 6144 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291212.1, NP_001278141.1
• RefSeq Size: 7396 bp
• RefSeq ORF: 6144 bp
• Locus ID: 360013
• UniProt ID: Q9JMH9
• Cytogenetics: 11 B5
• Gene Summary: May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.