Scn10a (NM_001205321) Mouse Untagged Clone

CAT#: MC229685

Scn10a (untagged) - Mouse sodium channel, voltage-gated, type X, alpha (Scn10a), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scn10a
• Synonyms: Nav1.8; PN3; SNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229685 representing NM_001205321
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCCCCTTTGGGTCCGTGGGAACTACCAACTTCAGACGGTTCACTCCAGAGTCGCTGGCAGAGA
  TCGAGAAGCAGATCGCTGCCCACCGCGCCGCCAAGAAGGGCAGACCTAAGCAAAGAGGACAGAAGGACAA
  GAGTGAGAAGCCCAGGCCTCAGTTGGACTTGAAGGCCTGTAACCAGCTGCCCAGGTTCTATGGCGAGCTC
  CCAGCAGAGCTGGTCGGGGAGCCCCTGGAGGACCTGGATCCTTTCTACAGCACACACCGGACATTCATAG
  TGTTGGATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCTCTGTGGCTCTTCAGTCCCTTCAA
  CCTGATCAGAAGAACAGCCATCAAAGTGTCCGTCCACTCCTGGTTCTCCATATTTATCACTGTCACTATT
  TTGGTCAACTGTGTGTGCATGACCCGAACTGATCTTCCAGAGAAACTCGAGTATGCCTTCACTGTTGTTT
  ACACCTTCGAGGCTCTGATAAAGATACTGGCAAGAGGGTTTTGTCTAAATGAATTCACTTATCTTCGAGA
  TCCCTGGAACTGGCTGGACTTCAGTGTCATTACCCTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA
  ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGGGCCCTGAAGACTGTTTCTGTGATCCCAGGACTGAAGG
  TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACCATCCTCACAGTCTTCTGCCT
  GAGTGTCTTTGCCTTGGTGGGCCTGCAGCTCTTCAAGGGGAACCTCAAGAATAAATGCATTAAGAACGGC
  ACAGATCCGCACAAGGCTGACAATCTCTCATCTGAAATGGCAGGAGACATCTTCATCAAGCCCGGTACTA
  CGGATCCCTTGTTGTGTGGCAATGGATCTGATGCTGGCCACTGCCCTAATGATTATGTCTGCCGGAAAAC
  TTCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTCGCGTGGGCGTTCCTCTCACTGTTCCGT
  CTCATGACGCAGGACTCCTGGGAACGGCTGTACCAGCAGACACTCCGGGCTTCCGGGAAAATGTACATGG
  TCTTTTTTGTGCTGGTCATCTTCCTTGGATCATTCTACCTGGTCAATTTGATCTTGGCTGTGGTCACCAT
  GGCATATGAGGAACAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAGAAGAAGTTCAAGGAAGCC
  CTCGAGGTGCTGCAGAAAGAACAGGAGGTGCTGGCAGCGCTGGGCATTGACACAACCTCGCTCTATTCCC
  ACAACGGATCACCCTTAGCCCCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGATGTCAGA
  AGGCTCGACAGATGACAACAGATCACTACAATCCGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC
  CTTTCTTCTGGAAGACGCAGGGCTAGCCACAGCAGTGTGTTCCACTTCCGAGCACCCAGCCAAGACGTCT
  CATTTCCTGATGGGATCTTGGATGACGGGGTCTTTCATGGAGATCAGGAAAGCCGTCGAAGTTCCATATT
  GCTGGGCAGGGGTGCCGGGCAGGCAGGTCCTCTCCCCAGGAGTCCACTGCCTCAGTCCCCCAACCCTGGC
  CCTAGACGTGGAGAAGAGGGACAGCGTGGAGTGCCCACTGGTGAGCTTGCCACTGGAGCGCCTGAAGGCC
  CGGCACTCGATGCTGCAGGACAGAAGAACTTCCTGTCTGCAGACTACTTGAATGAACCTTTCCGAGCACA
  GAGGGCAATGAGTGTTGTCAGTATTATGACTTCTGTCATTGAGGAGCTGGAAGAGTCTAAGCTGAAGTGC
  CCACCCTGCTTGATCAGCTTAGCCCAGAAGTACCTGATATGGGAGTGCTGCCCCAAGTGGAAGAAATTCA
  AGATGGTGCTCTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTCACCATCACCCTGTGCATTGTGGTGAA
  TACCGTCTTCATGGCCATGGAACACTACCCCATGACTGATGCCTTCGATGCCATGCTCCAAGCCGGCAAC
  ATTGTCTTTACTGTGTTTTTTACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTACTACT
  TCCAGAAGAAGTGGAACATCTTCGACTGTGTCATCGTCACCGTGAGCCTGCTGGAGCTGAGCACATCCAA
  GAAGGGCAGCTTGTCTGTGCTCCGCACCTTCCGCTTGCTTCGGGTCTTCAAGCTGGCCAAGTCCTGGCCC
  ACCCTGAACATGCTCATCAAGATCATCGGGAACTCTGTGGGGGCCCTGGGCAACCTGACCTTCATCCTGG
  CCATCATCGTCTTTATCTTCGCCCTGGTGGGAAAGCAGCTCCTCTCAGAGAACTATGGGTGCCGCAGGGA
  TGGCATCTCCGTGTGGAATGGTGAGAGGCTGCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTCGTC
  GTCTTCCGGATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGGACTACA
  TCTGCCTCACCCTCTTCTTGACAGTGATGGTGCTAGGCAACCTGGTGGTGCTCAACCTATTCATCGCTTT
  ACTGCTGAACTCCTTCAGTGCGGACAACCTCACAGCCCCAGAGGATGACGGGGAGGTGAACAACTTGCAG
  GTAGCACTGGCCCGGATTCAGGTATTTGGCCATCGGGCCAGTCGGGCCATTACCAGTTACATCAGAAGCC
  ACTGCCGGCTCCGCTGGCCCAAGGTGGAGACCCAGCTGGGGATGAAACCCCCACTCACCAGCTGCAAAGC
  TGAGAACCACATTGCTACTGATGCTGTCAATGCTGCAGTGGGGAACCTGGCAAAGCCAGCTCTTGGTGGC
  CCCAAGGAGAACCACGGGGACTTCATCACTGATCCTAACGTGTGGGTCTCTGTGCCCATTGCTGAGGGGG
  AGTCCGACCTTGATGAGCTCGAGGAAGATGTGGAGCATGCTTCTCAGAGCTCCTGGCAGGAAGAGAGCCC
  CAAAGGGCAGCAGGAGCTGCTGCAGCAAGTCCAAAAGTGTGAAGATCACCAGGCAGCCCGAAGCCCACCC
  TCCGGGATGTCCTCTGAGGACCTGGCTCCATACCTGGGGGAGAGATGGCAGAGGGAGGAGAGCCCTCGGG
  TCCCTGCCGAGGGAGTGGATGACACAAGCTCCTCCGAGGGCAGCACGGTGGACTGCCCGGACCCAGAGGA
  GATCCTGAGGAAGATCCCTGAGCTGGCGGAGGAGCTGGACGAGCCCGATGACTGTTTCCCAGAAGGCTGC
  ACTCGCCGCTGTCCCTGCTGCAAAGTGAACACCAGTAAGTTTCCTTGGGCCACGGGCTGGCAGGTGCGCA
  AAACCTGTTACCGCATTGTGGAGCACAGCTGGTTTGAGAGTTTCATTATCTTCATGATCCTGCTCAGCAG
  TGGAGCGCTGGCCTTTGAGGATAACTACCTGGAAGAAAAGCCCCGAGTGAAGTCTGTGCTGGAGTACACT
  GACAGAGTGTTCACCTTCATCTTTGTATTCGAGATGTTGCTCAAGTGGGTAGCTTATGGCTTCAAAAAAT
  ATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTCATCGTGAATATCTCCCTCACAAGCCTCATAGCCAA
  GATCCTCGAGTATTCAGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGGCCGCTGCGG
  GCTCTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCTTGGTGGGCGCCATTCCCTCCATCATGA
  ACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGTGTGAACCTCTTCGCCGGGAA
  ATTTTCGAGATGTGTCGACACCAGAAGCAACCCATTTTCCGTCGTGAATTCGACATTCGTGACTAACAAG
  TCTGACTGTTACAATCAAAACAATACTGGCCACTTCTTCTGGGTTAACGTCAAAGTCAACTTCGACAACG
  TTGCTATGGGCTACCTCGCGCTTCTCCAGGTGGCAACCTTCAAAGGCTGGATGGACATTATGTATGCAGC
  TGTCGATTCTCGAGATATCAACAGTCAGCCCAATTGGGAGGAGAGCCTGTACATGTACCTATACTTCGTC
  GTCTTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTCGGGGTCATCATTGACAACTTCAATC
  AACAGAAAAAAAAGATAAGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCAT
  GAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTTTGAATAAGTACCAGGGCTTCGTG
  TTTGACATTGTGACCAGGCAAGCATTTGACATCATCATCATGGCTCTCATCTGCCTCAACATGATCACCA
  TGATGGTGGAGACCGACAATCAGAGCGAGGAGAAGACGAAGGTCCTGGGCAGAATCAACCAGTTCTTCGT
  GGCCGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTTCGGCAGTATTACTTCACCAACGGCTGG
  AATGTGTTCGACTTCATTGTGGTGATTCTGTCCATTTCTAGTCTGTTGTTTTCTGCGATCCTTAGCTCAC
  TAGAAAGTTACTTCTCCCCCACGCTCTTACGCGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCT
  GATTCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAAC
  ATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAATGTCA
  TAGATGAGGCTGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTTTTCCA
  GATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGGACCCCCCTACTGCGAC
  CCCAACCGGCCCAACAGCAATGGCTCCAAGGGGAATTGTGGAAGCCCAGCGGTGGGCATCCTCTTCTTCA
  CCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCAGTGATTCTGGAGAACTTCAA
  TGTGGCCACAGAAGAGAGCACGGAGCCCCTGAGCGAGGACGACTTTGACATGTTCTATGAGACCTGGGAG
  AAGTTTGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTTGCAGACACACTCTCTG
  GCCCTCTTAGAATCCCAAAACCTAATCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGGAGA
  TAAGATCCACTGTTTGGACATCCTCTTTGCCTTCACAAAGAATGTCTTGGGAGAATCTGGGGAGTTGGAT
  TCTCTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGCATCCTATGAACCAATAG
  CAACCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAGGCCTATCGGAACTACAT
  GTTGCAACGCTCCTTGATGCTCTCCAACCCCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCACTC
  CCCAGGGAAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGACAAATCGGAAACTGCTT
  CTGCTACGTCTTTCCCACCATCCTATGACAGCGTCACCAGGGGCCTGAGTGACAGGGCCAACATTAGCAC
  ATCTAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAAGAGCCCTGGACCTCAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001205321
• Insert Size: 5877 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205321.1, NP_001192250.1
• RefSeq Size: 6416 bp
• RefSeq ORF: 5877 bp
• Locus ID: 20264
• Cytogenetics: 9 71.33 cM
• Gene Summary: Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.