Taf1 (NM_001290729) Mouse Untagged Clone

CAT#: MC229682

Taf1 (untagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf1
• Synonyms: AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; p250; Taf2a; TAFII250
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229682 representing NM_001290729
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCGGGGTGGGCTGGGCTGCTGCAGGACAAGGGTGGCGGTAGCCCTTCCGTCGTCATGTCCGACA
  CAGACAGCGACGAAGAGTCGGCTGGTGGAGGCCCATTTTCTCTAACTGGGTTCCTGTTCGGCAACATCAA
  TGGAGCAGGGCAGTTGGAAGGCGAAAGCGTCTTGGATGACGAGTGCAAGAAGCATCTTGCGGGCTTGGGG
  GCTTTGGGTCTTGGCAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCCGGGAGTGATGGTGCCC
  TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAGGATGCTGTGGACTATTCTGACATCAACGAGGTGGC
  AGAAGATGAAAGTAGAAGATACCAGCAGACAATGGGAAGCTTGCAACCCCTTTGCCACACAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCTCCACCTCCACCCCCAG
  GACCACTGAAGAAAGAGAAGGACCAAGATGATATTACTGGTGTGTCTGAAGATGGAGAAGGCATCATCTT
  GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA
  TCTGAGATGGGACCTCAGGACGCAGCACAGTCAGAATCTAAGGATGGACAGCTGACCCTACCATTGGCTG
  GGATTATGCAGCATGATGCCACGAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTAGGCCTGG
  AAAGGTGTTACGCTTTTTACGGCTTTTTGGACCGGGGAAGAATGTTCCATCTGTTTGGAGGAGTGCTCGG
  AGAAAGAGGAAAAAGAAGCACCGTGAGCTTATACAGGAAGGGCAGGTCCAGGAAGAGGAGTGCTCAGTGG
  AATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCTCCTCCACTTCCAGACCAGTGTCT
  CTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGACACAGAT
  AAAGTAATGGATACCAAGCCAAGAGTGGCCGAATGGCGTTATGGACCTGCCCGGCTGTGGTATGATATGC
  TAGGTGTTCCTGAAGATGGCAGTGGCTTTGATTATGGCTTCAAAATGAAGAAGACTGAACATGAATCTAC
  AATAAAATGCAACATAATGAAGAAATTAAGGAAACTTGAGGAAAACAGTGGCGTCGATCTTCTGGCAGAT
  GAAAACTTCCTAATGGTGACACAGCTGCATTGGGAAGATGATATCATTTGGGATGGGGAGGATGTCAAAC
  ACAAAGGAACAAAACCTCAGCGTGCGAGTTTGGCAGGCTGGCTTCCTTCCAGTATGACTAGGAATGCCAT
  GGCCTACAATGTGCAGCAAGGTTTTACAGCTACATTGGATGATGATAAACCTTGGTACTCCATTTTCCCC
  ATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGGATGCTCAGAACATGCCTA
  GAATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGATTTTGGAGATCCCTGATGA
  GAAGGAAGAAGCTACTTCTAATTCTCCTTCTAAGGAGAATAAGAAAGAATCATCTCTGAAGAAGAGTCGA
  ATTCTCTTAGGAAAAACAGGAGTCATCAAGGAAGAGCCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG
  ATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGCCTTCGAGGTACTTTTGGAGG
  AAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTTCCTACCCACATGGGACCC
  ATCAAACTTCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGGCACTATCTCAGCCAGGTC
  CACACTCAGTTCAGCCTTTGCTGAAGCACATCAAAAAGAAGGCAAAGATGAGAGAACAAGAGAGGCAAGC
  CTCGGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGATCTCACAGGCAAAGATGGAGACCTTATT
  CTTGCTGAATACAGTGAGGAAAATGGACCATTAATGATGCAAGTTGGCATGGCAACCAAGATAAAAAACT
  ACTATAAGCGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATACGGAGAAACTGTTTATTGCCA
  TACATCGCCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTTGAGAACAACCTTTTTCGT
  GCTCCAATTTATCTTCATAAGATGCCAGAATCTGACTTCCTGATCATTCGAACAAGGCAGGGTTACTTTA
  TTCGAGAATTAGTGGATATTTTTGTTGTTGGCCAGCAGTGTCCCTTATTTGAAGTTCCTGGTCCTAACTC
  CAAAAGGGCCAATACACACATCCGAGATTTTCTTCAGGTTTTTATTTACCGGCTCTTTTGGAAGAGTAAA
  GATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCACATTCAGAAAGCAGCATCC
  GGAAGAGGCTAAAGCTCTGTGCCGACTTCAAACGTACAGGGATGGATTCAAACTGGTGGGTGCTGAAGTC
  TGATTTTCGTTTACCAACGGAAGAGGAGATCCGAGCTATGGTATCCCCAGAACAGTGCTGTGCTTATTAT
  AGCATGATAGCTGCTGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGTCCTTTTTTGCTCCTGAGG
  AAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGCTCCTTGGAATACTACAAG
  GGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTAGCAGATCCTACAGGCTGT
  GGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCGACACAACAGAAGGATGATAAAGAGCCTCAGC
  CAGTGAAAAAGACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTGTCTCTGAAAAATGCCAAGCAGCT
  TCTTCGTAAATTTGGTGTGCCTGAGGAGGAGATTAAAAAGTTGTCCCGCTGGGAAGTTATTGATGTGGTA
  CGTACAATGTCAACAGAACAGGCTCGCTCTGGAGAGGGGCCCATGAGTAAATTTGCTCGTGGATCAAGGT
  TTTCAGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT
  TTTGTCATCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGACAGTGACTTTGAAGAA
  ATGGGAAAGAATATTGAGAATATGCTGCAGAACAAGAAAACAAGTTCTCAGCTGTCTCGGGAGCGGGAGG
  AGCAGGAACGGAAGGAACTCCAGCGGATGCTACTGGCAGCAGGCTCAGCAGCAGCAGGAAACAATCACAG
  AGATGATGACACAGCTTCAGTGACCAGCCTTAACTCTTCTGCCACTGGCCGCTGTCTCAAGATTTATCGA
  ACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTTGTGAGACAGTCCGAAAAGCAACTGTTATTGATG
  CTTATGTACGCATACGGACCACAAAAGATGAAGAGTTCATTCGAAAATTTGCCCTTTTTGATGAGCAACA
  CCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAAGAGCAATTGAGGAGACTTAAACGGAACCAGGAA
  AAGGAAAAGCTAAAGGGGCCTCCTGAGAAAAAGCCCAAAAAAATGAAGGAACGGCCTGACTTAAAATTGA
  AATGTGGAGCATGTGGTGCCATCGGGCACATGAGGACAAATAAATTCTGCCCCCTTTATTATCAAACAAA
  TGCTCCACCTTCTAACCCTGTTGCTATGACCGAGGAACAAGAGGAAGAGCTGGAAAAGACAGTCATTCAT
  AATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAGATCGTTTTGGGCAAACAGCTAATTGAGAGTG
  CAGATGAAGTTCGCAGAAAATCTCTGGTCCTGAAGTTCCCTAAACAACAACTTCCCCCCAAGAAAAAACG
  ACGGGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAATCCATCCACCGGCGCCGGACA
  GACCCGATGGTGACACTGTCTTCTATCTTGGAGTCTATTATCAATGACATGAGAGATCTTCCAAATACAT
  ACCCTTTCCATACTCCAGTTAATGCAAAGGTTGTAAAGGATTACTATAAAATCATCACTCGACCAATGGA
  TCTACAGACACTCCGTGAAAACGTGCGCAAACGCCTTTATCCGTCTCGGGAAGAATTCAGAGAACATTTG
  GAGCTAATTGTGAAAAACAGTGCAACTTATAATGGACCAAAGCACTCACTGACTCAGATTTCTCAATCCA
  TGCTGGATCTCTGCGATGAAAAACTCAAAGAGAAAGAGGATAAGTTGGCACGTTTAGAGAAAGCTATCAA
  CCCCTTGCTTGACGATGATGATCAAGTAGCCTTTTCTTTTATTCTGGATAACATTGTCACTCAGAAAATG
  ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAG
  TGATTGTCAGTCCAATGGATTTGGAGACTATACGTAAGAATATCTCCAAGCACAAATACCAGAGTCGAGA
  GAGCTTTCTAGACGACGTAAACCTGATTCTGGCCAACAGTGTTAAGTACAATGGGCCTGAGAGTCAGTAT
  ACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACCAGACATTAACTGAGTATGATGAACATTTGACTC
  AACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGCTCTGGAGGAAGCAGAATTAGAAAGTCTGGACCC
  AATGACTCCAGGGCCATATACACCTCAGGCTAAGCCTCCTGATTTGTATGATAACAACACATCCCTCAGT
  GTTTCTCGAGATGCTTCTGTATATCAAGATGAGAGCAATCTGTCTGTCTTGGATATTCCCAGTGCTACTT
  CAGAAAAGCAGCTAACACAGGAAGGTGGAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGT
  ACAACAGCCCCAAGCCAGTGTCTTGTATGAGGATTTGCTTATGTCTGAAGGCGAAGATGATGAGGAAGAT
  GCTGGGAGTGACGAAGAAGGAGATAACCCTTTCTTTGCTATTCAGCTAAGTGAAAGTGGAAGTGACTCTG
  ATGTGGAATCTGGCAGTTTAAGACCCAAACAGCCCCGTGTGCTTCAAGAGAATACAAGGATGGGCATGGA
  AAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGACGCTTCCCGTGGTTTGGAGGATAGCAAC
  ATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCCAACACCCAGGATACCAGCTTCAGCAGCATCG
  GTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCAGCGCTCTGGGCCCAGCGTACTAAGCCA
  GGTCCACCTCTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCCGGAGACACTGATTTGGAC
  TCTGATGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290729
• Insert Size: 5682 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290729.1, NP_001277658.1
• RefSeq Size: 8033 bp
• RefSeq ORF: 5682 bp
• Locus ID: 270627
• UniProt ID: Q80UV9
• Cytogenetics: X 44.29 cM
• Gene Summary: Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:10438527). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.[UniProtKB/Swiss-Prot Function]