Ubr3 (NM_001303033) Mouse Untagged Clone

CAT#: MC229681

Ubr3 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 3 (Ubr3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubr3
• Synonyms: 1110059H15Rik; 4833421P10Rik; A130030D10Rik; AA409735; AA414972; AA422631; AI646861
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229681 representing NM_001303033
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCCGCGGCGGCCGTCGGGGACCCGCAGCCGCCCCAGCCGGAGGCGCCGGCGCAGG
  GGCTGGCTTTAGACAAAGCGGCCACCGCCGCTCATCTGAAGGCCGCTCTCAGCCGGCCGGATAACCGTGC
  GGGCGCCGAGGAGCTGCAGGCGCTGCTGGAGAGGGTGCTGAACGCCGAGCGGCCGCTGGCCGGGGCTGCG
  GGCGGCGAGGAGGCGGCGGGCGGCGGCGGCGGCGGCCCGGGCGAGGCGGAGGAGGATGCCCTGGAGTGGT
  GTAAGTGCCTTCTGGCCGGCGGCGGCGGCTACGAGGAGTTCTGCGCGGCCGTGCGGGCCTACGACCCCGC
  GGCGCTCTGCGGCCTGGTCTGGACGGCCAACTTCGTGGCCTACCGCTGCCGGACGTGCGGCATCTCCCCC
  TGCATGTCGCTGTGCGCGGAGTGCTTCCACCAGGGCGACCACACTGGACACGACTTCAACATGTTCCGCA
  GCCAGGCCGGGGGCGCCTGCGACTGCGGGGACAGCAACGTAATGCGGGAGAGCGGGTTTTGTAGAAGACA
  TCAGATTAAATCAAGTTCAAATATTCCCTGTGTCCCCAAAGACTTACTGATGATGTCTGAATTTGTTCTT
  CCAAGATTCATTTTTTGTCTTATTCAGTACTTAAGAGAAGGCTATAATGAACCAGCAGCTGATGCACCAT
  CAGAAAAAGATCTGAACAAAGTCCTTCAGCTTTTGGAGCCTCAGATTTCCTTTTTAGAAGACCTGACTAA
  AATGGGAGGAGCCATGAGGTCTGTTCTTACTCAGGTGTTAACCAACCAGCAGAACTACAAAGACCTGACT
  GCGGGTCTTGGAGAAAATGCTTGTGCAAAGAAAAGTCACGAGAAGTACCTTATAGCTTTGAAGAGCTCCG
  GACTGACATATCCCGAGGATAAGCTGGTGTATGGGGTCCAGGAACCAGCTGCTGGTACTAGCACTCTGGC
  AGCTCAAGGTTTTGCAGGTGCTACTGGAACACTGGGGCAGATAGACTCTTCAGATGAGGAGGACCAGGAT
  GGGAGTCAAGGCTTGGGCAAGAGAAAGAGGGTGAAGCTAAGCAGTGGCACCAAAGATCAATCCATAATGG
  ATGTTTTGAAACATAAAAGTTTTTTAGAAGAACTATTATTTTGGACAATAAAGTATGAGTTCCCACAGAA
  GATGGTAACTTTCTTGCTCAATATGCTCCCAGATCAAGAGTATAAGGTTGCTTTTACAAAAACATTTGTT
  CAGCATTATGCTTTTATTATGAAAACACTGAAGAAAAGTCACGAATCAGATACAATGTCCAACAGAATTG
  TGCATATTAGTGTTCAGTTGTTCAGCAATGAGGAGCTAGCCAGACAGGTGACAGAAGAGTGTCAGCTCCT
  GGACATCATGGTCACTGTGTTGTTGTACATGATGGAGAGCTGCCTCATCAAGAGTGAGCTGCAAGATGAA
  GAGAACAGTTTACATGTAGTGGTGAACTGTGGAGAAGCCTTACTGAAGAATAACACCTACTGGCCCCTTG
  TCAGTGATTTTATTAATATTCTTTCTCATCAAAGTGTGGCTCAGAGGTTTCTGGAGGATCACGGCCTGTT
  AGTTACATGGATGAACTTTGTGTCTTTCTTTCAAGGTATGAACTTGAACAAGCGAGAGCTCAATGAGCAC
  GTGGAATTTGAGTCTCAGACCTACTATGCTGCCTTTGCTGCTGAGCTGGAAGCCTGTGCACAACCCATGT
  GGGGGCTTTTATCACATTGTAAAGTTAGGGAAACTCAAGAGTATACCAGGAATGTTGTAAGGTACTGCCT
  TGAAGCTCTTCAAGATTGGTTTGATGCTATTAACTTTGTAGACGAGCCAGCTCCTAACCAAGTCACTTTC
  CACCTACCACTGCACCGCTACTATGCTATGTTTTTGAGTAAGGCTGTGAAATGTCAAGAACTAGATTTGG
  ATTCACTTTTACCGGATCAGGAAATGTTAATGAAACTAATGATTCACCCACTCCAAATTCAAGCTAGTCT
  TGCTGAAATTCACAGTAACATGTGGGTAAGAAATGGTCTGCAAATCAAAGGACAAGCCATGACCTATGTC
  CAGTCTCATTTCTGTAACTCCATGATTGACCCAGACATTTACTTACTACAGGTCTGTGCTTCTAGACTTG
  ACCCAGACTACTTTATATCATCTGTCTTTGAAAGATTTAAGGTGGTGGACTTACTGACCATGGCTTCACA
  GCACCACAACATGGTGCTCGATGTGGAGCACGAGCGGTCGATGCTAGAGGGTGCTCTCACGTTCCTCGTG
  ATTCTTTTGAGTCTTCGTTTACACCTAGGGATGTCTGACGATGACATTCTCAGGGCAGAGATGGTGGCCC
  AGCTGTGTATGAACGACAGGACACATAGCTCTTTGCTTGACCTCATCCCAGAAAATCCCAACCCCAAAAG
  TGGCATTATTCCTGGAAGTTATAGCTTTGAATCAGTTTTATCTGCTGTAGCGGACTTTAGGGCTCCCATT
  TTTGAACCTGGAGGCTCTATGCAACAAGGCATGTACACTCCCAAAGCTGAAGTCTGGGATCAGGAGTTTG
  ATCCTGTCATGGTCATTCTTCGAACCGTTTACCGCCGAGACGTGCAGTCTGCAATGGACAGATACACAGC
  ATTTTTAAAACAGAGTGGAAAATTCCCTGGCAATCCCTGGCCTCCGTATAAGAAAAGAACATCACTCCAT
  CCCAGCTATAAAGGTCTTATGAGACTTCTGCACTGTAAAACTTTACACATCGTGCTCTTCACTCTGCTTT
  ACAAGATTCTGATGGATCATCAAAATCTGTCTGAGCATGTGCTGTGCATGGTCTTGTACCTGATTGAGTT
  AGGCCTGGAGAACTCTGCTGACGATGACTCCGAGGAAGAGGTATCAATGGGTGGACCAGAGCGTTGTCAT
  GACAGTTGGTTCCCTGGCAGTAACTTGGTATCAAACATGAGGCACTTTATAAATTATGTTAGAGTGCGAG
  TCCCAGAGACGGCTCCTGAGTTGAAAAGGGATCCCCTCGCCAGCACGAGCTCTGACGCTTTGGACTCTTT
  ACAAAATTCTGGTACAGCTCAAGTTTTCAGCTTAGTAGCAGAGCGTAGAAAGAAATTTCAGGAGATCATC
  AATCGCAGCAACAGTGAAGCAAATCAGGTGGTTCGGCCCAAGATTCCAAGTAAATGGTCAGCTCCTGGTT
  CCTCTCCCCAGTTAACCACAGCCATTTTGGAAATTAAGGAAAGCATATTGTCTTTGCTAATAAAACTTCA
  CCACAAACTCTCAGGAAAACAAAACTCTTACTATCCTCCATGGCTTGATGACATAGAAGTTTTAATACAA
  CCAGAAATTCCCAAATACAATCATGGAGATGGTATAACTGCTGTAGAAAGGATTTTGCTAAAAGCTGCAG
  TGCAAAGCAGAATGAACAAGCGCATCATTGAGGAGATATGCAGAAAAGTGACCCCTCCTGTGCCACCCAA
  AAAAATCACCGCAGCGGAGAAGAAAACTCTGGACAAGGAGGAAAGGCGACAAAAGGCTAGAGAAAGGCAG
  CAGAAATTGCTCGCAGAGTTTGCCTCACGACAGAAAAGCTTTATGGAAACTGCAATGGATGTTGATTCTC
  CTGAGAACGATATTCCTATGGAAATTACCACAGCAGAACCACAAGTTTCTGAGGCCGTGTATGACTGTGT
  TATTTGTGGACAGAGTGGTCCTTCTTCTGAAGACAGACCTACAGGACTGGTTGTACTGTTACAAGCATCA
  TCAGTTTTAGGCCAGTGCCGGGACAATGCTGAGCCAAAAAAGTTGCCGATCGCTGAAGAGGAGCAGATTT
  ACCCTTGGGACACATGTGCAGCAGTTCATGATGTGAGGCTTTCATTACTGCAGCGTTATTTTAAAGATAG
  TTCTTGCCTTTTGGCAGTATCAATTGGCTGGGAAGGAGGTGTTTATGTACAAACTTGTGGCCACACGTTG
  CACATAGATTGTCATAAATCCTATATGGAGTCGTTACGGAATGACCAGGTTCTTCAGGGCTTCTCAGTGG
  ACAAAGGAGAATTCACGTGTCCCCTCTGTAGACAGTTTGCCAACAGTGTTCTTCCATGTTATCCTGGAAG
  CAATGTGGAAAATAACCTTTGGCAACGCCCTTGTAACAAAAGCACGCAAGATCTCATAAAGGAAGTGGAA
  GAGCTGCAGGGACGGCCGGGAGCTTTCCCAGTAAGCATCAGTTCAGAAACCAACTTAAGTAAAGAAATGG
  AATCTGTAATGAAAGATATAAAAAATACCACTCAGAAGAAATACAGAGACTATAGCAAGACCCCAGGCTC
  ACCAGACAACGAGTTTCTCTTTATGTACTCTGTTGCTAGAACCAATCTGGAGCTGGAACTGATTCATAGA
  GGAGGCAGTCTCTGCTCCGGTGGTCCAAGCACAGCGGGGAAAAGATCTTGTCTGAATCAGCTGTTCCATG
  TATTAGCCTTGCACATGCGGCTTTATACCATTGATTCTGAATATAATCCATGGAAAAAACTCACCCAGTT
  AGTGGAAGATATGAATTCACAGGTGGGGAATGAAGACCAGCAGCCAGAAGTTCCAATTCTTTATCATGAT
  GTGACATCCCTTCTGCTCATCCAGATCTTAATGATGCCACAACCCTTACGTAAAGAGCACTTCACCTGCA
  TTGTGAAAGTACTCTTCACCCTGCTGTACACACAGGCCCTGGCGGCGCTCTCAGTTAAGGGCACTGAAGA
  GGACCGGTCAGCCTGGAAACACGCCGGGGCTCTTAGAAAGGATACATGTGATGCAGAAAAGTGTTACGAA
  GTGCTGTTGAGCTTTGTTATAAGTGAGCTGTCTAAAGGGAAGTTATATTATGAAGAAGGCGCTCAGGAGT
  GTGCGATGGTCAGTCCTATTGCTTGGTCTCCTGAGTCCATGGAAAGATACATACAGGATTTCTGCTTGCC
  TTTCCTCAGGGTCAGCAGCCTTCTTCAGCACCACCTCTTTGGGGAAGACTTACCCAGTTGCCAGGAAGAA
  GAAGAATTTTCAGTCCTTGCCAGCTGCCTGGGACTTCTGCCAACTTTTTACCAAACAGATCACCCGTTCA
  TCAGTGCCTCGTGTCTGGACTGGCCAGTTGCAGCGTTTGACATTATAACTCAGTGGTGTTTTGAGATAAC
  ATCATTTACGGAGAGACACGCAGAGCAAGGAAAGGCCTTGCTTATCCAAGAATCAAGATGGAAGTTACCA
  CACCTGTTACAGTTGCCTGAGAATTACAACACTATTTTTCAGTACTATCATAGAAAAACCTGCAGTGTCT
  GCACCAAGGTTCCTAAAGACCCAGCTGTTTGCCTCGTCTGCGGCACTTTCGTATGCCTGAAAGGCCTGTG
  CTGCAAGCAGCAAAGTTACTGTGAATGTGTGCTGCATTCTCAGAATTGTGGTGCAGGAACAGGAATTTTC
  CTTTTAATCAACGCGTCAGTGATCATCATCATCCGAGGCCACCGCTTTTGCCTCTGGGGTTCCGTCTACT
  TGGATGCTCATGGAGAAGAGGACCGGGACCTCAGGCGAGGCAAGCCTCTCTACATCTGTGAGGAGCGGTA
  CAGAGTTCTCGAGCAGCAGTGGGTTTCCCACACGTTTGACCACATCAATAAAAGATGGGGTCCGCACTAC
  AATGGGCTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001303033
• Insert Size: 5682 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303033.1, NP_001289962.1
• RefSeq Size: 8098 bp
• RefSeq ORF: 5682 bp
• Locus ID: 68795
• Cytogenetics: 2 C2
• Gene Summary: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (PubMed:17462990). Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (PubMed:17462990). May play a role in Shh signaling by mediating the ubiquitination of Kif7 (PubMed:27195754). May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A (PubMed:27331610).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents the longer transcript and encodes the longer isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.