Ank1 (NM_001277286) Mouse Untagged Clone

CAT#: MC229674

Ank1 (untagged) - Mouse ankyrin 1, erythroid (Ank1), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ank1
• Synonyms: Ank-1; nb; pale
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229674 representing NM_001277286
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCGGCCGCGCAGGTCGGGGTCAGACCCCGCGGCCGATGCTGCTACCAGCTTTCTGCGGGCGG
  CACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGACATTAACACCTGTAACCA
  GAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCAC
  AAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACATCGCTGCCCTTGCTGGTC
  AGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAGTCTCAGAAAGGCTTTAC
  TCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTACTGGAGAATGGAGCCAAT
  CAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCAGGGTCACGAGAATGTGG
  TGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTGCACATCGCGGCCCGCAA
  CGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATGTGCTTTCCAAGACGGGA
  TTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTTGCTCCTCAACAGGGGAG
  CCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCCCGCAGGGGGAACGTGAT
  CATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGGATGAATTGACACCGCTC
  CACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCACGGGGCACCCATCCAAG
  CCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCACCTCGACTGTGTCCGACT
  TCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTCCTCTCCATGTGGCAGCC
  CACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCCCAACTCCAGAGCCCTGA
  ATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATGGAGTTGCTGCTGAAGAC
  AGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAGCCTCCTTCATGGGACAC
  CTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAATGTGAAAGTAGAAACCC
  CCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGC
  CAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCGGCCACACAGGCATGGTG
  AAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCACACACCCCTACACACCG
  CAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCATCCCAAGCCTGCATGAC
  CAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGTTGGCGGAGCTGCTGCTG
  GAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCATGTGGCCGTCCATCACA
  ACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGCCCTGCCTGGAATGGCTA
  CACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAGTACGGAGGG
  TCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCAAGAGGGCCACACAGAAA
  TGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGTGGACTCACTCCCCTCCA
  CCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATGGCGTCACCGTGGATGCT
  ACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACATCAAGCTGGTGAAGTTCT
  TGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCTTGCACCAGGCAGCTCA
  ACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAAC
  GGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCA
  CAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCTGAGACGGTGGACGAGAT
  CCTGGACGTGTCTGAAGATGAAGGAACTGCTCACATATCTATAATGGGTGACGAACTTGTCGGCTCGAAG
  GCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACC
  AAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACCGTTGTGATCCGATCTGA
  AGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCAGCCCGGCCACAGAGACC
  TCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAGCTTCATGGTGGATGCCC
  GAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCTCCCCGGACATGCGCAGC
  ACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCACCCCCACTGGCTGAGGAA
  GAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGG
  AAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTGAGGAGTGAAAACGGCTC
  TGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCCTTAATGGCATGGATGAA
  GAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCACCACCGACTTCCCTCTGT
  ACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGTTCCCTGAGAAG
  CAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGAAAGTGAAGCTGGCTCTG
  CAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGCCACATTCAGCCCCATTG
  TGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATCCCGCTCCCTCCCTCATG
  GACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTACTGTGTAGCGTCATCGGT
  GGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATACGCCAATGAGTGTGCCA
  ACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACTGCGGAGGCTGTGCACTT
  TGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTGTCATTTTTGCCAAGATG
  AACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGTGGACAAGACCCTGGAGC
  AGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAAGGGATGCCTCTGTTTGC
  AGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCTTCCATTTCCAGTCGTTT
  CGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGT
  TCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTGAATATCACCATGCCCCC
  CTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATT
  CTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAG
  AACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTGGAAGACATCAACCGGAT
  CCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCCTCTGGGTGGACCGTGAA
  GGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAGGAGTGAGATTGTTAACA
  TGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACATGGGGACCGGGAGTACTC
  ATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGTCCCCCGCCTCCCTGCAG
  TACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGTCATAGACGCCATCCCCC
  TGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGGTCTGCGGGCCTCACACC
  CTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGAGGACTCTGACGCCATACCAGAG
  TGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTCTCAGGACCTGGTGGAGG
  ACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGTCAGGAGGAAGGCCAGCAGCG
  AGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAGGTGCTGGACAGAAGCCAGGCCAGAACACTG
  GACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAGAAGCCACACAGAGCTCCTGGCAAGAGGAGG
  TCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCACCATCCAAGGGCCGGAGCCTGGTGCGCTTCA
  GGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTGCAGAGGGGGCCACCTGAGACCGGCAGCCCC
  AAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCGCCTTCTCTCAAGAGGTGCAGGGGGATGAGC
  TTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATTCACAGATGAACAGGGCAACATTGTTACCAA
  GAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCTGGTGCCATCGACACCCAGCAGCACGAGGAG
  GTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAGAGGGCAGGAAGGGGGCTCAGATAGTGAAGC
  GGGCCAGCCTGAAAAGGGGCAAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277286
• Insert Size: 5559 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277286.2, NP_001264215.1
• RefSeq Size: 8191 bp
• RefSeq ORF: 5559 bp
• Locus ID: 11733
• UniProt ID: Q02357
• Cytogenetics: 8 11.42 cM
• Gene Summary: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) differs in the 5' UTR, represents use of an alternate promoter, initiates translation at an alternate start codon, and lacks an alternate in-frame segment compared to variant 1. The encoded isoform (6) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1.