Plxnb2 (NM_001284506) Mouse Untagged Clone

CAT#: MC229672

Plxnb2 (untagged) - Mouse plexin B2 (Plxnb2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plxnb2
• Synonyms: Debt; plexin-B2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229672 representing NM_001284506
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCCACTCTGGGCCCTGACCTTTCTAGGTCTCACGGGCCTAGGGTTGAGCCTGCGGTCCCGAA
  AGCCGGAGAGTTTCCGCAGTGAGACAGAGCTGAACCACCTGGCTGTGGATGAGGTCACAGGTGTGGTGTA
  TGTCGGGGCAGTGAACGCGCTCTACCAGCTGAGTGCCGACCTGCACGTCCAGCAGCACGTGGTCACAGGC
  CCCTTCATGGATAACAAGAAGTGCACACCACCCATTGAGGCCAGCCAGTGCCACGAAGCGGTGCTCACTG
  ACAACTTCAACCAGCTGCTGCTGCTCGACCCACCGGGGAAACGCCTGGTGGAGTGCGGCAGCCTCTTCAA
  GGGCATCTGTGCCCTGCGCGCCATGAGCAACATCTCGGTGCGCCTCTTCTACGAGGATGGCAGCGGCGAG
  AAGTCCTTCGTGGCCAGCAATGACGAGAGAGTGGCCACCGTGGGGCTGGTGACCTCCACGCGCCCCGACG
  GCGAGCGCGTGCTGTTTGTGGGCAAAGGCAATGGGCCGCACGACAATGGTATCATCGTGAGCACCCGCCT
  GCTGGACAGGGCTGAGGGCCGGGAGGCCTTTGAGGCCTACTCTGACCACACCACCTTCAAGGCCGGCTAC
  CTGTCCACGAATACTCAGCAGTTTGTCGCAGCCTTTGAGGATGATTTCTATGTCTTCTTCGTCTTTAACC
  ATCAAGACAAGCATCCTGCTAAGAACCGCACGCTGCTGGCGCGCATGTGCAAAGACGACCCCTCCTACTA
  TTCCTATGTAGAGATGGACCTGCAGTGTCAGGATCCCAGTGACCCCCAAGACTCTGCCTTTGGCACCTGC
  CTAGCCGCTTCTGTAGCCACATCGGGTGCTGGCAGAGCACTTTATGCTGTCTTCAGCAGAGATGGCCGGA
  GCACTGGGGGACCTGGCGCGGGCCTCTGTGTGTTCCCGCTAGATAAAGTCCGTGAGAAGATAGAAGCCAA
  CCGTAACGCCTGCTACACAGGTGCCCGAGAGGCAGGCCGCACTATCTTCTACAAGCCCTTCCACGGAGAA
  ATCCAGTGTGGCGGCCATCTGATAGGTGCCAGCGAGAGCTTCCCATGCGGCTCGGAGCATCTGCCCTATC
  CACTGGGCAGTCGTGATGGACTCGTCGCCACAGCCGTGCTGCACCGCGGGGGCCTGAATCTGACAGCAGT
  GACAGTAACTGCCGAGAATGACCACACTGTTGCGTTCCTGGGCACCTCAGATGGCCGGATCCTTAAGGTG
  TACCTTGCTCCAGACGGCACTTCTGCAGAGTATGGTTCTATCCCAGTAGACATCAATAAGAAAATCAAGC
  AGGACCTGGCACTGTCTGGAAACCTGTCCAGTCTGTATGCTATGACCCAGGACAAGGTGTTCCGGCTCCC
  AGTACAAGAATGCCTGAGCTATGTAACCTGCGCTCAGTGTCGTGACTCCCAAGATCCCTACTGTGGCTGG
  TGTGTCATCGAGGGACGATGCACCAGGAAGTCCGAGTGCTCACGGGCCGAGGAAACCGGACACTGGCTGT
  GGAGCCGGGAGAAGTCCTGTGTGGCCATCACTGATGCCTTTCCACAGAACATGAGCCGGCGGGCCCAAGG
  AGAGGTACGCCTGTCTGTCAGCCCCCTGCCCACCCTGACTGAGGACGATGAGTTACTGTGCCTCTTTGGT
  GACTCACCACCCCACCCTGCCCGGGTAGAGGACGATACCGTCATCTGTAATTCCCCAAGCAGCATCCCTA
  GTACACCGCCAGGCCAAGACCATGTTGACGTGAGCATCCAGCTCCTCTTAAAAAGCGGCAGTGTCTTCCT
  CACCTCCCACCAGTATCCCTTCTATGACTGCCGTGAGGCCATGAGCCTGGTGGAGAACCTGCCGTGCATC
  TCTTGTGCTAGCAACCGCTGGACTTGCCAATGGGACCTGCAGTACTACGAGTGTCGGGAGGCTTCGCCCA
  ACCCAGAGGAGGGAATCATACGTGCCCACATGGAGGACAACTGCCCCCAGTTCCTGGCCCCTGACCCTTT
  GGTCATCCCCATGAACCATGAGACAGAGGTGACCTTCCAGGGCAAGAACTTGGAGACTGTGAAGGTTTCT
  TCCCTGTATGTGGGCAGTGAGCTGCTGAATTTTGAAGAGACTGTGACCATGCATGAATCAGACACCTTCT
  CTTTTAGGACCCCAAAGCTATCCCATGATGGTAATGAGACACTGCCTCTTCACCTGTATGTTAAGTCCTT
  TGGCAAGAACATTGACAGCAAGCTACAAGTGACTCTCTACAACTGCTCCTTTGGCCGCAGTGACTGTAGC
  CTGTGTCTGGCTGCCGATCCTGCCTACAGGTGTGTGTGGTGCCGTGGGCAGAACCGGTGCGTGTACGAAG
  CTCTGTGCAGCAATGTCACTTCTGAGTGCCCACCACCAGTTATCACTAGGATCCAGCCTGAGACGGGCCC
  GCTGGGTGGGGGCATCCTGGTCACTATCCATGGGTCCAATCTGGGTGTCAAAGCAGATGACGTCAAGAAG
  ATAACTGTGGCTGGCCAGAACTGTGCCTTTGAACCAAGAGGGTACTCCGTATCCACCCGGATTGTGTGTG
  CAATTGAGGCTTCGGAGATGCCCTTCACAGGAGGCATTGAGGTGGATGTTAATGGAAAACTCGGCCATTC
  ACCGCCACACGTCCAGTTCACTTATCAACAACCCCAGCCTCTCAGTGTGGAGCCACGACAGGGGCCACAG
  GCAGGTGGCACCACATTGACCATCAATGGCACTCACCTGGACACAGGCTCCAAGGAGGATGTGCGGGTGA
  CACTCAATGACGTCCCTTGTGAAGTGACAAAGTTTGGAGCACAGCTACAGTGTGTCACAGGTCAACAGTT
  GGCTCCAGGCCAGGTGACACTAGAAATCTACTATGGGGGCTCCAGAGTGCCCAGCCCCGGCATCTCTTTC
  ACCTACTGCGAGAACCCCATGATACGAGCCTTTGAGCCATTGAGAAGCTTTGTCAGTGGTGGCCGGAGCA
  TCAACGTTACTGGCCAGGGCTTCAGCCTCATCCAGAAGTTTGCCATGGTTGTCATCGCTGAGCCCTTGCG
  GTCCTGGAGGCGGCGGCGGCGGGAGGCTGGAGCCCTGGAGCGTGTGACGGTCGAGGGCATGGAGTACGTG
  TTCTACAACGACACCAAGGTCGTCTTCTTGTCTCCTGCTGTCCCCGAAGAGCCCGAGGCTTACAACCTCA
  CCGTGCTGATACGGATGGACGGTCACTGTGCCCCGCTCAGGACCGAAGCCGGAGTCTTTGAGTATGTGGC
  AGACCCCACCTTTGAGAACTTCACGGGTGGCGTCAAAAAGCAGGTCAACAAGCTCATCCACGCCCGGGGA
  ACCAATCTGAACAAAGCCATGACTCTGGAGGAGGCAGAGGCATTTGTGGGTGCTGAGCGTTGCATTATGA
  AGACGCTGACCGAGACGGACCTGTACTGCGAGCCCCCAGAGGTGCAGCCTCCACCCAAGCGGCGGCAGAA
  GCGAGACACGGCACACAACCTACCTGAGTTCATTGTGAAGTTTGGCTCCCGAGAGTGGGTGCTAGGCCGA
  GTGGAGTACGACACGCGTGCGAGTGACGTGCCGCTCAGTCTCATCCTGCCTCTGGTCATGGTACCCATGG
  TGTTTATCATCGTGGTCTCCATCTACTGCTACTGGAGGAAGAGCCAGCAGGCCGAGCGCGAGTACGAGAA
  GATCAAATCCCAGCTCGAGGGCTTGGAGGAGAGCGTGCGTGACCGCTGCAAGAAGGAATTCACAGACCTG
  ATGATCGAGATGGAGGACCAGACGAATGACGTGCATGAGGCAGGCATCCCCACGCTGGACTACAAGACCT
  ACACCGACCGCGTATTCTTCCTGCCATCCAAGGATGGTGACAAGGATGTGATGATCACCGGCAAGCTAGA
  CATCCCTGAGTCACGGCGGCCCATTGTGGAGCAAGCCCTCTACCAGTTCTCCAACCTGCTTAATAGCAAG
  TCCTTCCTCATCAATTTCATCCACACCCTGGAGAATCAGCGTGAGTTCTCAGCTCGGGCCAAGGTCTACT
  TTGCATCACTGCTGACTGTGGCCCTGCACGGGAAGCTGGAGTACTACACGGACATCATGCGCACGCTCTT
  CCTGGAGCTCATGGAGCAGTATGTGGTGGCCAAGAACCCCAAGCTAATGCTGCGCAGGTCTGAGACAGTG
  GTGGAGAGGATGCTGTCCAACTGGATGTCCATCTGTCTGTACCAGTACCTCAAGGATAGTGCAGGGGAGC
  CTCTCTACAAGCTCTTCAAGGCCATCAAACACCAGGTGGAAAAGGGGCCCGTGGATGCTGTGCAGAAGAA
  GGCCAAGTACACCCTCAATGACACAGGCCTGCTCGGGGACGATGTTGAGTATGCGCCTCTGACCGTGAGC
  GTGATTGTTCAGGATGAAGGGATCGATGCCATCCCGGTTAAGGTCCTCAACTGTGACACCATCTCTCAGG
  TCAAAGAGAAGATCATCGACCAAGTGTACCGTACTCAGCCCTGCTCCTGCTGGCCCAAGCCAGACAGTGT
  GGTGCTTGAATGGCGTCCTGGGTCCACGGCCCAGATTCTGTCTGACTTGGACCTTACCTCCCAGCGGGAG
  GGCCGGTGGAAGCGCATCAACACCCTGATGCACTACAACGTCCGGGATGGAGCCACCCTCATCCTGTCTA
  AGGTGGGAGTCTCCCAGCAGCCAGAAGACAGCCAACAGGACTTGCCAGGGGAGCGCCATGCCCTTCTGGA
  AGAGGAAAACCGCGTGTGGCACTTGGTGCGGCCAACAGATGAGGTAGATGAAGGCAAGTCCAAGCGCGGC
  AGCATGAAGGAGAAAGAGCGGACTAAGGCCATCACAGAGATCTACCTGACGCGGCTACTCTCCGTCAAGG
  GCACACTGCAGCAGTTTGTGGACAACTTCTTCCAAAGCGTGCTGGCGCCTGGGCATGCGGTGCCACCCGC
  CGTCAAGTACTTCTTCGATTTCTTGGACGAGCAGGCAGAGAAGCATGACATCCGAGATGAGGATACCATC
  CATATCTGGAAAACCAACAGTTTACCACTTCGGTTCTGGGTGAACATCCTGAAGAACCCTCATTTCATCT
  TTGATGTCCACGTCCACGAAGTGGTGGACGCCTCCCTGTCAGTCATCGCACAGACCTTCATGGACGCCTG
  TACTCGCACGGAGCACAAGCTGAGCCGAGACTCTCCCAGCAACAAGCTGTTGTATGCTAAGGAGATCTCT
  ACCTACAAGAAGATGGTGGAGGACTACTACAAGGGCATCAGACAGATGGTGCAGGTCAGCGACCAGGACA
  TGAATACGCACTTGGCAGAGATTTCCCGGGCTCACACAGACTCCCTGAACACACTCGTGGCCCTACACCA
  GCTCTACCAATACACACAGAAGTACTATGATGAGATCATCAATGCTCTGGAGGAGGACCCTGCAGCCCAA
  AAGATGCAACTGGCCTTCCGCCTACAGCAGATTGCTGCTGCGCTTGAGAATAAGGTTACAGACCTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001284506
• Insert Size: 5529 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284506.1, NP_001271435.1
• RefSeq Size: 6469 bp
• RefSeq ORF: 5529 bp
• Locus ID: 140570
• UniProt ID: B2RXS4
• Cytogenetics: 15 44.68 cM
• Gene Summary: Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (PubMed:17554007). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (PubMed:29981480). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (PubMed:17554007). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (PubMed:19948886). Regulates the migration of cerebellar granule cells in the developing brain (PubMed:21122816). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (By similarity). Plays a role in axon guidance, invasive growth and cell migration (By similarity). May modulate the activity of RAC1 and CDC42 (PubMed:21966369). Down-regulates macrophage migration in wound-healing assays (in vitro) (PubMed:21966369).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein.