Tsc2 (NM_001286718) Mouse Untagged Clone

CAT#: MC229658

Tsc2 (untagged) - Mouse tuberous sclerosis 2 (Tsc2), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tsc2
• Synonyms: Nafld; Tcs2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229658 representing NM_001286718
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACCAACAAGCAAAGATTCCGGCTTGAAGGAGAAGTTCAAGATACTGTTGGGATTGGGAACAT
  CGAGGCCAAATCCCAGGTGTGCAGAAGGCAAACAGACTGAGTTTATCATCACATCGGAAATCTTGAGAGA
  ACTGAGTGGTGAATGCGGCCTCAACAATCGCATCCGAATGATAGGGCAGATCTGTGATGTGGCAAAAACT
  AAGAAGCTTGAGGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC
  CACCAGAGGCCCGGCATGCAGTTCTCACCTTATTGAAGGCCATTGTACAGGGACAGGGTGATCGTTTGGG
  AGTTCTCAGAGCCCTCTTCTTCAAAGTGATCAAGGACTACCCCTCCAATGAAGACCTCCATGAAAGGCTA
  GAAGTTTTTAAGGCCCTCACAGACAATGGGAGGCACATTACCTATTTGGAAGAAGAACTGGCAGAGTTTG
  TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTTGTCAACCTGGTCAAGTTCAA
  CAGCTGTTACCTTGACGAATACATTGCATCAATGGTTCACATGATTTGTCTGCTATGCATCCGGACAGTG
  TCCTCTGTGGACATTGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCTTACCAGCCG
  AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCATCAATGTCAAGGAGCTGTGTGAGCCTTGCTG
  GAAGTTGATGCGTAACCTTCTGGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCGCATCATG
  GAGGACAGATCCTACATGGAAGATGCCCCACTGCTGAGAGGAGCTGTGTTCTTTGTAGGGATGGCACTCT
  GGGGAGCTCACCGGCTCTACTCTCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCTAT
  GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTATAGG
  AAGGAGCTCCAGGCTGTGACATGGGATATTCTGCTGGACATCATTGAACGACTACTTCAGCAACTCCAGA
  ACCTGGACAGCCCGGAACTCAAGACCATCGTCCATGACCTGCTGACCACTGTGGAGGAGCTATGTGACCA
  GAACGAGTTCCATGGCTCGCAGGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGACCTGAA
  TCCTCTCTCTTAAACTTGATATCCTACAGAGCCCAGTCCATCCACCCTGCCAAGGATGGCTGGATCCAGA
  ACTTGCAGTTGTTGATGGAGAGGTTCTTCAGGAATGAGTGCCGCAGCGCCGTGCGCATCAAGGTGTTGGA
  TGTTCTGTCCTTCGTGCTGCTGATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCGGTGGTCATC
  TCGCAGCTCTCCCACATTCCCGAGGATAAGGACCATCAGGTCCGAAAGCTGGCTACTCAGCTGCTGGTGG
  ACCTGGCAGAGGGGTGCCACACCCACCACTTCAACAGTCTGCTGGACATCATTGAAAAGGTGATGGCGCG
  CTCACTCTCTCCACCCCCGGAGCTGGAAGAAAGGGACCTGGCCATGCACTCGGCCTCCCTGGAGGACGTG
  AAGACCGCGGTCCTGGGGCTCCTGGTCATCCTTCAGACCAAGCTCTACACCTTGCCTGCCAGCCACGCCA
  CTCGAGTGTATGAGAGCCTCATTAGTCACATCCAGCTCCATTACAAGCACGGCTACTCCCTGCCCATTGC
  TAGCAGCATCCGACTACAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC
  CTGCCCAACAAGGATGGGGTCGTGAGATTCAGCCCTTACTGCCTCTGTGACTGCATGGAACTGGATAGAG
  CCTCAGAGAAGAAAGCCAGTGGGCCCCTTTCACCTCCAACTGGGCCCCCTAGCCCTGTGCCTATGGGCCC
  TGCTGTGCGGCTCGGCTACCTACCCTACTCCCTGCTCTTCCGTGTCCTGTTGCAGTGTTTGAAGCAGGAG
  AGCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAGGCTGCCGGAGTCACTGCGCTACAAAGTCCTCATCT
  TCACCTCCCCCTGCAGTGTCGACCAGCTGTCTTCTGCCCTCTGCTCCATGCTTTCAGCTCCAAAGACCCT
  TGAGCGGCTCCGAGGTACCCCAGAAGGCTTCTCGAGAACTGACCTGCACTTGGCTGTGGTTCCCGTGCTA
  ACAGCATTAATATCTTATCACAACTATTTGGACAAGACCAGACAGCGTGAGATGGTGTACTGCCTGGAAC
  AAGGCCTTATCTACCGCTGCGCCAGCCAGTGCGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA
  CATCATCATCAAGGCGCTTCCTGTCCTGGTGGTGAAGCTCACACACATTTCTGCCACAGCCAGCATGGCC
  ATCCCGCTCCTGGAGTTCCTGTCTACTTTGGCCAGGCTGCCCCACCTCTACAGGAACTTTGCTGCAGAGC
  AGTATGCCAGCGTGTTTGCCATCTCCTTGCCATACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG
  TCTGGCTCACCACGTCATAGCCATGTGGTTCATCAGGTGCCGACTGCCCTTTCGGAAGGATTTCGTCCCT
  TATATCACTAAGGGTTTGCGTTCCAATGTCCTCCTGTCTTTTGATGATACCCCTGAGAAGGACAGTTTCA
  GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGAATACAGACATCCCTTACGAGCGCCAGCCTGGG
  GTCTGCGGATGAGAACTCTATGGCCCAGGCTGATGACAACTTGAAGAATCTCCACTTGGAGCTCACAGAA
  ACATGTCTGGACATGATGGCCCGATATGTGTTCTCCAATTTCACTGCAGTCCCCAAGAGGTCCCCTGTGG
  GAGAGTTCCTCCTGGCAGGGGGTAGGACCAAAACCTGGCTGGTTGGAAACAAGCTTGTCACTGTGACAAC
  AAGTGTGGGAACTGGTACACGGTCGCTGCTGGGCCTGGACTCTGGGGACCTGCAGGGTGGCTCGGATTCA
  AGCTCTGATCCTAGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTGGGC
  AGCAGGTGTCCCGTGGGGCCCGGGACCGGGTCCGCTCCATGTCAGGGGGCCATGGCCTTCGAGTTGGTGT
  CCTGGATACTTCAGCTCCCTATTCCCCAGGTGGCTCTGCTTCTCTGGGACCACAGACGGCGGTGGCAGCG
  AAGCCCGAGAAGCCCCCTGCAGGAGCCCAGCTTCCAACAGCAGAGAAGACGAATCTGGCAGCCTATGTGC
  CTTTGTTAACCCAGGGCTGGGCAGAAATCTTAGTCCGCAGACCCACAGGAAACACCAGCTGGCTGATGAG
  CTTGGAGAACCCGCTCAGCCCCTTCTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCCAATGCC
  CTTATGGCTGCTGAACGTTTCAAGGAGCACCGGGACACGGCCCTGTACAAGTCATTGTCAGTGCCAGCGG
  CTGGCACCGCCAAGCCTCCTACTCTCCCACGCTCTAATACAGACTCAGCCATGGTCCTTGAGGAGGGAAG
  TCCAGGAGAGACTCAGGTGCCAGTGGAGCCCCCCGAATTGGAGGATTTCGAGGCGGCGTTAGGTACAGAC
  AGGCACTGCCAGCGGCCTGACACCTACAGCCGGTCATCCTCAGCATCTAGCCAGGAAGAAAAGTCCCATT
  TGGAGGAGCTTGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTGCCGT
  GGACCTCTCCTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCGGAGTTGCAGACCTTACAG
  GACATCCTTGGAGACCTAGGGGACAAGATTGATATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGGTCCC
  AGTCAGGGATCCTGGATGGGGAAGCAGCTACCTGGTCAGCTACGGGTGAAGAGAGCCGTATCACAGTCCC
  ACCTGAAGGTCCTCTGCCTTCCAGTTCTCCCCGCTCCCCCAGTGGCCTCCGGCCCCGAGGCTATACCATC
  TCTGATTCCGCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGATAACTTCAAGAGCAGAGCTGCGGCCT
  CCAGTGCTGAGAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCGCCCTTTTT
  TGGTGATGAGTCCAATAAGCCCATCCTATTGCCCAATGAGTCCTTTGAACGGTCAGTACAGCTGCTTGAC
  CAGATTCCATCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTGAGC
  TGGCCATCCTGTCAAATGAGCATGGCTCTTACAGGTACACGGAGTTTCTGACAGGCCTGGGTCGGCTTAT
  TGAGCTCAAGGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTATGTGGCGAGGATGGACAG
  TTCACCTACTGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCCACCCTGATGCCCACCAAGG
  ATGTGGACAAGCACCGCTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAATGA
  TTCTGGTGAGGACTTCAAACTGGGCACCATTAAGGGCCAGTTCAACTTTGTCCATGTGATCATCACACCG
  CTGGACTACAAGTGCAACCTATTGACCCTGCAGTGCAGGAAAGACATGGAAGGCCTCGTGGACACCAGTG
  TGGCCAAGATTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATATGGC
  CTCACAGGTACACCACAGCCGATCCAACCCCACTGACATCTATCCCTCCAAGTGGATCGCAAGACTCCGC
  CACATTAAGCGTCTCCGCCAGAGGATCCGTGAAGAGGTGCACTATTCCAACCCAAGCTTGCCTCTGATGC
  ACCCTCCAGCCCACACCAAAGCCCCAGCTCAAGCCCCTGAGGCTACACCCACCTATGAAACAGGCCAGCG
  GAAGCGCCTCATTTCCTCCGTGGATGACTTCACAGAGTTTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286718
• Insert Size: 5226 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286718.1, NP_001273647.1
• RefSeq Size: 6134 bp
• RefSeq ORF: 5226 bp
• Locus ID: 22084
• Cytogenetics: 17 12.41 cM