Ddx60 (NM_001293783) Mouse Untagged Clone

CAT#: MC229656

Ddx60 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (Ddx60)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx60
• Synonyms: 9830118M07
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229656 representing NM_001293783
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAAAATACACAGGTTTTTTAAAAATAATGTCACATTTAATCTTGGAAACTATGTCAAAGGCTG
  ACTACTCCAGCTTATTCAATGATTTTGTTGAATCTGAGTTCTTTCTGATTGATGGCGACTCCTTGCTCAT
  CACGTGTGCGTGTGAGAGGTCACTTAAGCCAGGACAGAGTCTCCACTTCTTCTACCTGGTTGAGCGCTAC
  CTGTTGGATGTTATTAGCAAAGGAGGGCAATTTGCCATAGTTTTCTTCAAGGATGCTGAGTATGCATACT
  TCAATGTCCCCGAACTTCTGACACTGAGAACTGCTTTAATTCTGCATCTTCAGAAGAATACCACTGTTGA
  AGTTTGGACTAAGTTTTCTAACTGCTTGTCAGAAGAGTGGAATATTTGCTTGGGTCAGAGTTGCCCATAT
  TTCTTGATACTGGCTGATGAAGGCCTGAACGACCTGCAGACACATCTTTTCAACTTTGTAGTTATTCAGG
  CCTGGGCAGCAAAGGTCAATATTGTGCTCTTCTCAGGACAGACATCTGACATCCTTCGTCTTTATGCATA
  CTTTATGCAAAGCTCTCATGGAATGCAGATGTTCTTCAAGGAGAATAAGAGAATGATCATGATTGTTTAC
  AAAAGCCTGATTCAACAGCTGGAAAAATACAGAGATATGGCTTTAGCACATCTGTTCGGAGATTTAAAAT
  TCAATGATATGGAAGAGAAGGTATGTGAAACTGTATCTCTGCTGAAACAACTCTGGCCAGAAGGATCTGA
  CATCCGATGTGTCTTTTGTGTCACCTCATGTTCTCTCTTCTTGGAAATGTATCACTGCTACTTAGAAAGC
  AGAGAGAAAGGAGCATCTGAGGAGATGTCCAAAGTCAAACAGAAAACAGATAATTGCTTAACCCTGAAGG
  ACATGGAAGACTTTTGTAAGCTACATTGCCTCAGTGTGGCTCTTTTGCTCCATGTACCTCTCTCTCAGAG
  AGCTCGCTCAAGATTCATCACTGCTAATTGGATTCAGGACCTCAAGACTGTCCTAAAAATGAAAAAGTGG
  TGCGAGTATTTTATCCTAAGTAATTTGGATATGTTTGAATCTTGGAGTCTGAATTTACTTCACCTTTCTG
  ACTTAAGTGATGAGCCTTTGTTGAGGAATATTGCTTTTTACTATGAAAATGAAAATAAAGAAGGCCTGGA
  TTTGAATGTGGGAGATGCCATCATGAACGAGTATGTACATCTCTGGGACACTGTGTCAAAGTTGGTCAGA
  AAGTTTGATGTGGGAAAACCAATTCCTTTGAGAACAACAAAAGTTCATTTTCTTGAAAAGACACCATCAC
  CAATCAAAGAAAGTTCCCAGGAGATGATGCCAAATTTAGGTTTTATTCCAATGTCATGTAATTTAGTTGA
  TGAATTTACTGGAGACATTTTGAAAGACTTGCCTTTCCTAATGAGTGATGACCCCATTGTTACGTCACTG
  GTAAAGCATAAGGAATTTGATGAGCTTGTGCACTGGCACTCCCACAGACCTCTCAGTGATGATTATGACA
  GGACAAAGGTGAATTCTGGAGACAGAAGAGACCCCAATCATCTTAAAAGGTTGCAGAAGTATCATAGTTT
  TCAACGATTTTATGGGGAGTCATTAGAATCAGTCACTTCAAAGACCATCACAACTCAAGGTCCTAAGCCA
  AAGAAGGATTGTATTAAGTCCAAGAGCACAAAGGCACATGAGACCAAGGCTGAAATAATTAAAAGAGAAA
  ATAAGAAGAGGTTAATTGCTGAGGAAGAACAAAAAGAAGAGCAAAAATGGAATGCCCTGTCATTTTCTAT
  CGAGGAGGAAATGAGAAAGAATGTAGACTCAGGAATGAAGAAACTGGAAGAGTTTTTGAAATCATGTAAA
  AGTCGCTCAGTAAAAGCTCAGGTTGAAAGAGTTGGGTTACAGGCTTGCTTGAAAGCCTGGAAGGAACATT
  GCCAGGCTGAAGGTGAAACCACGAAAGACTTAAGCATAGCTGTCAATATGATGAAGAGGATCCATTCCTT
  GTTGGACAAACACTCTGAACTTCTGCAAGAAGCAGATCAGAAACTCATAGCCAGATGTCTTAAGTATTTA
  GGGTTTTGTGAGCTAGCAGTTTCTTTATATCCAACTCAGGATGCATCAGATGGCATAAAAATGAAGAAAA
  AGAATAAATATTCAGTTGGGATTGGGCCGGCTCGGTTCCAACTACAATATATGGGTCATTATTTAATACG
  AGAAGAAAGAAAAGACCCAGACCCCAGGGTCCAAGATTTTATTCCTGACACATGGCAGCGAGAGCTCCTG
  GATGTGGTGGATAACTATGAGTCTGCAGTAATTGTTGCCCCAACATCATCTGGCAAGACTTATGCTTCCT
  ACTATTGCATGGAAAAGGTGCTGAAAGAGAGCGATGAAGGCGTGGTTGTGTATGTTGCACCAACAAAGGC
  TCTTGTTAATCAAGTGGCAGCAACTGTTGAATATCGTTATGCCAAAAATATGCCAGCTGGTGAATCTCTT
  TGTGGTGTTTTTACCAGGGATTATCGCCATGATGCCCTCAACTGTCAGGTGCTTATAACTGTTCCTGCCT
  GCTTTGAAATTCTGCTACTAGCTCCTCATCGCCAAAATTGGGTGAAGAGGATCAGATATGTTATATTTGA
  TGAGGTCCATTGCCTTGGTGGAGAAATTGGAGCGGAAATCTGGGAGCATCTCCTTGTTATGATCCGATGT
  CCCTTTTTGGCCCTTTCAGCTACCATTAGCAACCCTCAGCATCTCACCGAGTGGCTTCAGTCAGTAAAGC
  GGTACTGGAAGCAAGTAGACAGTACCATGGAGGAAAGAACTGTTTCCAAAAGAACTGGTACCTCCCGTGG
  CAGCTATTACAAAGATCAAGCGCAAGCCAGACAGTCCTACAAAGTTAGACTTGTGCTCTATGGAGAGAGA
  TACAATGACCTAGAGAAGTATTTGTGTTCCGTGAGACAGGGCGATGTTTGCTTTGATCATTTTCACCCCT
  GCGCTGCACTAACCATAGATCACATTGAAAAGTATGGATTTCCTTCTGATCTTGCCCTTTCACCCCGAGA
  AAGCATACAGCTGTATGACACTATGTGCCAGGTCTGGAAGAGTTGGCCCCAGGCCCAGAGCCTATGTCCA
  GAGAACTTCACTCAATTTAAGAATAAAATAGTCATTAAGAAGTTGGATGCTAGAAAATATGAAGAGAGCT
  TAAAGGAAGAATTCACGAATTGGGTTAAAAATGGGAACGAGGAGGAGGCCAGGATGGTGCTGAAGAAACT
  GAGCCCTGATTACCATGAGCACTCAGGGAGGATGCTGGATTTCTTTCCATGTCTTGTTGAGAAGCTAAGG
  AAAATGGAGAAGTTGCCTGCACTATTCTTTTTATTTAAGTTAAATATTGTAGAAGAATGTGCTGAAAATG
  CTTTCGAATTTCTGGAGAAAAAACAGGAAGAAAAAAGACCTCCCAAAGCTGACAAAGAAGCTCGTACCAC
  GGCTAACAAACTTCGGAAAGTTAAAAAATCTCTGGAGAAACAAAAGACAGTAGATGAAAAGGGCCAGAAA
  TCTTCCAGAAGATTGGATGAAAGCGTTATGCATGAAACAGAACACAATTACCTCCTGCAGACTCTGGAGA
  AGAATCTGGAGATTCCCAAGGACTGCACATATGCTAATCAGAAGGCAATAGATGATCAGTTATTGCAGAG
  GGTGTTTCACCGGGTGAGATTCGAACGTAAAGGTGAAACACTGAAGAGGTTGGCAGAAAGGGGCATTGGC
  TACCACCACAGTTCCATGAGTGCCAAAGAAAAGCAGTTGGTCGAAATTCTCTTCAGGAAAGGATTCATCA
  GGGTGGTGACAGCTACTGGAACACTCGCTTTGGGAATCAACATGCCCTGTAAATCTGTGGTTTTTGCACA
  AAACTCAGTCTATCTGGATGCTTTAAATTACAGACAGATGTCTGGTCGGGCTGGAAGACGAGGGCAAGAC
  CTGTTGGGTGATGTCTACTTCTTTGATATTCCACTGCCCAAAATAGGAAAACTCATAAAATCCAAAGTTC
  CTGAACTTAGAGGACAGTTTCCCCTCAGTATTTCACTCATCCTAAGACTCATGTTGCTGGCTTCCAAGGC
  GGATGACCTAGAGGATGGCAAGGCAAAGGCACTGTCAGTGCTGAAGCATTCATTGTTATCCTTCAAACAA
  CCCCGAGCCATAGACATGTTAAAGCTTTACTTCTTATATTCTTTGCAGTTCCTGGTGAAAGAGGGACATA
  TAGATCAAGAAGGTAATCCTACAGGGTTTGCTGGCCTTGTCTCCCATTTACATTACCACGAACCTTCTAA
  TCTCGTGTTTGTCAGTTTTCTTGTAAAAGGCCTTTTCCATAAACTTTGTCAGCCAACAAGGGAAGGCCCA
  AAACGTTTTTCTGAAGATGTAATGGAAAAGCTGGTTTTGGTATTGGCAAATCTTTTTGGAAGAAGATATA
  TCCCAGCCAAGTTCTATGAGGTTAACTCCAAGTTTTATCAATCAAAGGTGTTCCTTGATGACCTTCCCGA
  GGACTTCAGTCACGCCCTGCTTGAGTACAATACGCAAGTGACAGAAGACTTCGCTTCCTTTCTGCTAATG
  GTATCCCGATTGGCTGATATGAAACAGGAGTATCAACTCTCCTTGTCAAAAATCGAGTTCACAGGTAAAG
  AATGTGAAGATTCGCCACTTGTGTCTCACTTGATGAACTGCAAGGAAGGAAGGGTGGCGGTTTCACCCTT
  TGTTTGTCTGTCTGGAAACTTTGATGTGGATTTGCTTAGTCCAGGAGTTCCAAATCATGTCATTCTACAC
  ACACTTGGTATCAATCACACACAAGCCCCGGTGCTGTGGCCATACGCATTGGATAGTCAAGGAAGAAAAA
  TTCCACTTAATGCCTATGCACTTGATTTCTATAAGCATGGATCCCTTATTGGATTAGTCCAGGACAATAG
  GATACATCCAGGGGAAGCTTATAGCCTGTTGAAAGATTTTAATCTTATCATTAAGTCTATCAGTGTCTCC
  TTGCGTGAACTGTGTGAAAATGAAGAAGACAATGTTGTTTTAGCATTTGAACAATTGTCTACGTCTTTTC
  AAGAGAAGTTTGAATTAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293783
• Insert Size: 5133 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293783.1, NP_001280712.1
• RefSeq Size: 5994 bp
• RefSeq ORF: 5133 bp
• Locus ID: 234311
• Cytogenetics: 8 B3.1