Wnk3 (NM_001271679) Mouse Untagged Clone

CAT#: MC229655

Wnk3 (untagged) - Mouse WNK lysine deficient protein kinase 3 (Wnk3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnk3
• Synonyms: Prkwnk3; Wnk3-ps
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229655 representing NM_001271679
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTGAAA
  ACAGGGCTGCCCGGGCTGTTGCCCCTCTGACTGTAGAAGCGAGAATAAAGGAAAAATACAGTACCTTTTC
  TGCTTCCGGGGAAAACATCGAAAGAAAGAGGTTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA
  GTTGCCGAATCTTCCCGCAGAGATGAAAGAAAAGCTGCAACAAATATTTCAAGAGTAGATAAAGTTCCTA
  CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAATGTTCAAAGGCAACCTCAGAATCCTCAAA
  AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTTGCAACTTCC
  CCTAGTGGCAGGTTCCTGAAATTCGATATAGAGCTGGGAAGAGGAGCATTTAAAACGGTATATAAAGGAT
  TGGACACTGAGACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA
  GCAACGGTTCAAAGAAGAAGCAGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTCTATGAT
  TCATGGGAGTCCACTTTGAAAGGAAAGAAATGTATTGTGTTAGTGACTGAACTAATGACATCAGGAACCT
  TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATCTT
  AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT
  TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCGT
  TTGCTAAGAGTGTCATTGGCACTCCTGAATTTATGGCTCCGGAGATGTATGAAGAACACTATGATGAATC
  TGTAGATGTTTATGCTTTTGGAATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC
  CAGAATGCAGCACAAATATACCGGAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG
  ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCTATCAAGGA
  CTTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC
  TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA
  ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA
  GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGAGTGACATTGATT
  AAAAAGATAAGGGAAAAAAAGCCTGCTGGATGCTTGGAGGAACGCAGGGATTCCCAGTGCAAGTATGTGA
  GGAATGTACTTCCTCAACAGCAGACTGCAACTTTACAACCTACTCCTGGTCCACACACTGCAGCTGAGTA
  TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGTTTCTACAGGGCAAACCACAGCAGCAGTCCTCC
  TCTGTTAGGGGTGACACTTCATCTGAGCCAACAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCATC
  CCACTGTAGCCTATTCTTCAAACCAGACAACTAGCTCTCAGGAGCAGCCAAAGCTGACTCAGTCCCCAGT
  TTTGCCTGTGGTTCAAGGTCAGTCCTCTGTTATGCCCATATATGCTGCTGGAGTTGGAGTTGTTTCACAG
  TCCCAAATCTCTCCCTTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG
  AACAACAAGCAACTCTTCAAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA
  AAACACCAATAACCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGAACAAACAACGAAGAGCCTCC
  TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGAGACAACA
  TGGTGGAGTGTCAGCTGGAAACACATAACAACAAGATGGTGACATTCAAGTTTGATGTTGATGGTGACGC
  ACCTGAGGATATCGCTGACTATATGGTTGAAGATAACTTTGTGCTGGAAAATGAGAAAGAAAAATTTGTC
  GAGGAATTGAGGGCTATCGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTCAGCTGTAGAAAAATCTA
  TTGGTGTTGACTCTGTTGCCTTGGAATCTAACAGTAACCAAACAGGATCATCTGAACAAGTACTGATAAA
  TTCTGCATCCACTCAAACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGT
  ATCAACCAAACAATAAAAAACCGTGAGGCACAGTCTCCTCCTTCCCTTCAGCCTTCCATGGCCATGGTGC
  CTGGGTTACATCCATTTCCTAGTTCAAGAAACACAAGTAATCAGGCAATATCACAGAACACAGTGTTCAC
  TATAGAGAATAATCCAGGCCATCGTGAACTTTTCACCTCCAAATTAGACCACAAGGATGTGGTTGATGGT
  AAGATTGGTGAACATGCTAGTATAGAAACTGAGCAGTCAAGTATTTCCTATCAAGTGGAAGATGACAGAC
  AGATCATGACACCAGCTACTGATAATTCCAATTATTCTGCTGCTTTGGTGTGTCCAGTTCCAGGTGAATG
  TGAGGCACTCACCAGCCAAGCAGGCATGTTCATGCCTACATATCCAAATCAGCAAGCTGCTGTTCTGGCT
  GATGTGCATATAGCCTATCCCGGTGAATCAGTTCCAATTGGTGGTAATGCTGCTCTTACATCAGTACTTG
  TATCTTCTGATCAAAAGCCTCAATCCTTGTCAGTACAGCAGCCAACTATAGATGCAGAGTTTATTTCCAA
  AGAAGGAGAAACCACAGTAAACACCGAAACAAGTTCTCCTAAGGCAGTCATTGCCACTCAGACTCCTGGC
  TTTGAACCAGCTGTGATTCTACCTGCTACTATCCTGGAATCAGATGGGGAACGACCTCCAAAAATGGAGT
  TTGCAGACAACCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTATCAGGAGCACAGCATTTC
  TAGCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTTTCTTCCTCTGCTGAAGAT
  ATACTATCCTATTCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTCAAGATAGCCCTGCACAAT
  CCCCAAATTTCCAACAAACAGGCTCTAAGATTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTCATATATC
  AGTGTTCAAAAAAGACCTGAATGTGATAACTTCTGTACCCAGCGAGTTATGTTTACATGAGATGTCCCCA
  GATGCTTCACTTCCAGGCGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGATGGAACCATTCATCTGC
  AGACAGGAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCATTCCTCTGACACGGGAGTT
  CTCATCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCAG
  GTGATTACTGTTCCTCAGCAGCAGCCAGTAAAAATGATGTCTTTTGGGAAAGATCACAGACCGCCATTCA
  ACAAAACAACAGTCCAGTCTAGTGAGCAAGCACTAACCTTTGCTGAAGCTGCAGTGTCGCAGTTGATCGA
  AGTGGAGCCTGCCATGCCCACTCATAAAGCTTCAGTTTCTTCACGCAAGTTGCGAACTCTTTATGAAACC
  TTTAAAGAAGATAAAGGAGATCCTGAACAAGGTGACATCGTATCTTACAGCACAGCTTGTGAGACCAGTG
  TCTCTTCAGTCGCTACAGAAAAGAATGTAACTTCAACCACAGAAGTTAGTGTGCAGTCTGGGTCTGAACC
  GTTAGATAAAGAAAAAAATGAGTCAACTCCTGGGAAACAGACATGTACAAATGAATTTTCAGCCACTCTT
  GCTGGTAATAGAAAGTCCGTGACAAAAACTCGTCCAGAGGGTGATCAGTATTTACCACTCCGTGAAGAGC
  AAGCCTATGCTCAAACACAGAATTCACTCTTCTATTCTCCATCTTCCCCAATGAGCAGTGACAATGAATC
  AGAAATTGAGGACGAGGACTTGAAAGTGGAGCTTCAAAGATTACGAGAAAAACACATTCAAGAGGTGGTC
  AGTCTTCAAACACAACAGAATAAAGAGCTACAAGAGCTCTATGAACGCCTTCGAGCAACTAAAGACAACA
  AAGCGCAATCTTCAGAGGTTCCTTTGTCACCTGCATCGCCACGCAGGCCAAGATCTTTCAAAAGCAAACT
  TCGAAGCCGCCCCCAGTCCATGACACATTCAGACAATCTCGTTGTAAAAGATGCACTGGGTGTGGAGAGT
  AATACCGTGTCATGCCAGCAATCTCCAGCCAGTAAGAAGGGGATGTTCACAGATGATTTACACAAGCTGG
  TGGATGACTGGACAAGAGAAACAGTAGGGCATTTTCCTAGTAAGCCAAGTTTAAACCAACTCAAACAGAG
  CCAACAAAAATCCGAAGCAGAAAACTGGAACAAGTCATGTGAAAGTACTCCATCTACTATGGGATACACG
  TCAAACTGGATTTCTTCTCTCTCCCAAATTCGTGGAGCTGCCCCAACTTCCTTGCCACAAGGACTCCCAC
  TCCCTTCATTTCATGGGCCATTAGCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGGTGC
  CGCAGGTTATCCAGTACAGTGGGTAGGAATTTCTGGACCAGCACAACAGTCTGTAGTGCTTCCTACCCAA
  TCTGGGGGACTCTTCCAGCCCGGGATGAATTTGCAGTCATTTCCAGCTCCACCAGTGCAGAATCCAGCTT
  CAATCCCTCCTGGTCCTAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271679
• Insert Size: 5133 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271679.1, NP_001258608.1
• RefSeq Size: 10259 bp
• RefSeq ORF: 5133 bp
• Locus ID: 279561
• UniProt ID: Q80XP9
• Cytogenetics: X F3
• Gene Summary: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternated splice site in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.