Topaz1 (NM_001199736) Mouse Untagged Clone

CAT#: MC229644

Topaz1 (untagged) - Mouse testis and ovary specific PAZ domain containing 1 (Topaz1)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Topaz1
• Synonyms: 671232; Gm9524
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229644 representing NM_001199736
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCCGCCAGCGACGCCCCCAGACCCGACCAAGGAGCCAGGGTGCAAAGGCACCACCCGAGGCCTGA
  GGAAGCGAGGCCTTCCGCTGACGCCAGAGCCAGGCGAGGGGGGAGGCTGCAGCCTGGAGGCCAGGGGGTG
  CGAGGAGGAGAGCAGGCAGAAGCAGAGGATGGTGACCCAGGCTTCTGGGAGAGAAGAGACAGAAGGTGAC
  AAGTTGGCCAAAGAGAATGGAAAAATTACTGAGGCCCCAAGTGATGATCCACAGCCAGGGACTGACCTGG
  TAAGAAAGACATCAATAACCAGTTCGGAGTCTTTGCAAACAGTGGAGTGCAGTGAATTTCAAAATATGGC
  TTTCTTACAGTCTTTGGATAAGGAAGAATTGGGAGAAGGTATTAAAAGAAGAATGCGAATTAAAAAATGT
  AAAAGTTTAGAAAATCCACCTCTTGAAATAACTAAAAATGAGGCAACACAAAATATTAAAGTTGAATTCC
  AAGATGAACTGTTCAAGAATACCCCAAAACATTCTTGTAACAGCTTATCACCTGGAGTAGAAAAAAATTG
  CAGCTTTGAATTGCATGATTACAGTTTTCTCCATTCTGAAGGTTGTAATAATGAAAACAACTTTGAGGAT
  AAACCTCATGATGTATGTCTACACACTGAAGAGAACTCTTTGAAGTTAAAGAAAGAAAACCTTAGGAATC
  TTGCAGAGAAAGATGACACAAGAACTACTACTAAGCTTTTAAAAACTGAAAAAAGTGTAATAGCCAGTAA
  GTTACTGCTTGAAGAAAGTCATTTATACCAAAATAAAAACAATGGCTTGACTTCTTGCCTTCAAAGTGAA
  AAAAACAAATATTCAGTAGAGGAGAACAACACTGGGAGAAAACACAGGAAAAAAATGAAGTCTGGAAAAG
  AAGAAAAAAATATTAATCTTACTTTGTCTAATGTATGTAACAATTCTGTGTTGGTGTTACAAGAAAACCA
  AATGGGTATGGAAGGGAAAGAAGCAGAGACCTTAGAACCTAAAAAAAGTTTTCTAAAAGCTTTAAGAAAA
  ATAAACCATAATACACTCCCTCCGGTGGACCATTTGTGTCTCCCCAAAACAGTGGGGAAAACAAGTTCTA
  GGCATCACATAAATGCTATGCTTCAGAAAACCCTCGAATCAAGTTTGAAAGAGGATATAAAAAATACTTC
  AGAGTCCTTAGGATGCAAACGTATAGAGCCAGAAAAATATAATAAATCGATGATAAGCTCTACAGTGAAA
  TCACCTAGTGACGGCCATCATATGGAAAAGAGATCTCCACGGGGAGATCTGAGAAGTGAAACTGAAGAGT
  CAAAGGTTAGCTGTTGCAGAACAATACCAATGACTGGTAAAAGAGTTTGGCCTTTTTATTCTTGTGCTAG
  AATATCTGCCCAGTGTTGGAAAAAGACTTCCTTGTCAGATTTAAATTACTCACTTCTGGGGCCACTGGAA
  AATGTTAGGCAACATGATTCTATAATACACCAAATGAACCAGACTCACTTACCTGACTCTAAACTGTTAC
  AACCATCCTTAACAGAAAGAACTACTGAGTCCTCAAGAAAAGAAATGTATGATTCTGATTTAAGTTGTTT
  GTCTTCAGTTTCTTCAGTAGAATCTACTGTGATGGATATAAAGGAAGCTATGTCCCATGATAAAAAGACA
  AAGTTAGAGGAACCCAGCAGAAATGGGGCAGAGGTAGTTTCTAATGCAACTGAAGACACCCAGTTAAGTA
  ATATAACTCAAAGCTTAACAGGAAATAAGAAAAGGAAAGGGAATTTATCAAAACTTAATTTAACAGTTGC
  ATCCCAAGAGAGCCAGGAAACAAATAACTGTGCAAATAAAACTGTTCATCGAAAAGTTTGTATTACTAAG
  CAAACACTTGTGGCACCGGACTTGGTTAAAATATTAAACACGGGACGGCTGACTAATTTTAAAATCCCTT
  TACTAAAGAACAAGACAGGAAAAAGAGGAGCAGTAAGTGCCAGGTCATCTGAGAGAGAAGCTTATAGCCC
  CCTAGAGCTACTTGACAGTTTATCTGGAGTAGAGGCAAAACAAAATAGGAATAAGGAAAATATCTGTACA
  ACTTCAGGACCACAGTCTTTGAATTTACATAGTTGTATAGCTCCAGGGCAAGCTAGTTCTCATTCATTCT
  ACAATAAGAATTCCTGTACTTCTTCGAGCTTTACCAAGAAAGGTTACGATAATAAAGCATGTAATCACAT
  CTCTGAACCAGGAAATATTATTTCAAATAAGGAGTCTATTTCTATGAAAATTGAAAATAATACCTTTTCT
  TGTGATCTTGGATATATTGATCAAAGTTCCTTCTGCTCTAAGAAACAAGAAGCATTTGTGCCAATTTCCT
  CTGAAATTAGTGGTAGAAAAATGACTAAAAGCATTTCAGAACTCAAATTGGGGTTTCCAGATATTCTTAA
  AGCATATGAAGATGATGTCCTCTTGATTGATGTAATTCAAGATGATCCAGAGCTCTTTGGAATCTCCAGT
  GAGGGGGATCTTTCCTTTGCTTCAGAAGTCTCCAAGATAAGCCAGGAGCCCAGAGTATCTGAAGACCAAC
  CAACAGCAGATTTTAAGCACATGCACCTTCCAGGGAAAAAAGAACCAGGCGATTTGAGTAAAGAAGTTGC
  CTTGCTGGACCCTGGATTGCTGAAACTGGAGAGCTGTCCTTCCTTGTCAGCAGCAAAGGAGCCTCAGCAT
  GATCCTGAAGGAGCAGCCATCTCCTTAGAAGCCACTGAGGAGACTGTTGTAAGTGGAAGCCTAGAAGGCC
  TCAGTGAACAAGCGCGGGCTTCAGACTCAGACGCGAAGTGCATAAGTTCAGACAAGGCAACCGTTATGGA
  AGAACAAGAGAGTACTCATGAAATTTTCAAAAGCAAAGATTCTAGAAATGTGGAGTCAGCTACTGAGTGT
  CAGTTAGCAACACTGGGCCCCAACCCTCTGTGCTCATCAGCACTTCCTGTGAATCTAAGCAGTCATCAAG
  ATGTAGTGTCAACACCCTGGATAAATGATGTCAGATTTCCAGGAAGACATTCTGTCCTACAACTGCAGAA
  CCCTGAAACTTGTGAAATATTTAAAAGAGAAAAAAACATGGGGGTGTTCCAGAAGCCCTTAGGGTTGTTG
  ATACCCCACAGATACTGCAAACTTCATTTTAACACATTGCGTGGCTGTGAGCGAGCACAGTGCAAGTTTG
  TCCATGTGCCTGAGCAGGGGGATGAGAAGATCTGCATGGATGTGTTTAGGAAATACATAAGTGTCAATGA
  GCAGCGTTTGCTGCATCGAGCAGCATACATATTTCTGGAGTACTACAGAAAGTTTCCCCCAGGGATACAC
  TTCAGTTTACAAGTGCTAAATGACCTTTTGATTTCTTTACTCAAGCATTGTTTATTGAAAGAAGTCTTCC
  AGGTAGTGCAGCTCAGCATAATGGCTAAAATGCTGCCTGCTCTGAAAATATTACTAAAAATATTTGAGTA
  TGTGGCAGCTATGAAGTTGAGGAATGCTGTACCTGCTTTAATTGAGATTTTTTGCAAGTTCATTGAAGCT
  GGGATGGTACCTGACCCAGAGCACTTGAACTATATTGTTAAGCTTTTACATCAAGCACAGGCATCCCAAC
  AAGAAATATCTGCAGTTCTGCAAGCCAAATCCAGGTTGCGAGTGAGGCAGCTGAAAAAGAACTGGAAGTG
  TGACTTAGACTCAGCCTTGAGTGAAGTAGAGACTTGTAAAGAAAAAAGTGACTGGACCAAATTGGGAAAC
  CTGTACATTAGCATAAAGATGAGTTGTGAAGAATTTGCAGATCTGCAGAGATTCTGTGCTTGTGTTGCTG
  AGACGCTCACAGAAGATTACAAAGAAGAGAGGCCAGGCGTTCCTTTTTGTGAATTTGCTGAAACAGTTAG
  CAAAGATCCACAGTACAGTGAGGTGGATAAGACTTTGTTGGGAAGGATCGGAATAAGTGCGGTGTACTTC
  TATCACAGACTGTTGCTGTGGGCCAAGGGAAGGAAGGTCTTAGATATATTATATGAGCTAAAAATACATT
  TCACAAGTTTAAAAGGACTTACAGGACCTGAAAAAGAAGCACCAAGATGTCAAATTGTAAATGTTGCAGC
  AGAGATTTTTATCAAAAGTGGGAGCCTAGATGGTGCCATTTGGGTCCTAAGGGAATCGGAATGGATAATC
  AACACACCACTTTGGCCTTGTGATAGAATGGATGTGCTTAATCGGCATAATTTGCTCTGTACCATTGCAC
  ATGAAATCTTGGGCAAGAATCTTTACAAACAAACATTTGAGGTCTTACGGAATCTACCAAGCTTTCAGAA
  TTCTCAAGAAATGATGGGGGTTTCCCAGTCCAGCCTTCTTTTTAATGAACTTCTGGATGCCTGTATAGAA
  AGTAACAGTCTTGGCATATCGTCTTCTGTAGCAGAGTTCATGGTTGCCAAGAGCATCCCTATTGACTTTT
  CCTTTCTCAGGAGACTCATCACTTCCTTAGGAAGGAGTTGTTTGTGGCTCAAAGCCAGAGCCCACTACAA
  AAGTGCTCTTTCACTGGGTTGCTACCCACCATTAGAGGGAAACTTACACCGGAAACTTCTCCTGGTTCCT
  TCGTATCTATCTGAGATTGAAATGCTTTTAGCTATGGAAATATTTCTGGTGTCCAATGCTAGTGGCATTC
  AGAGTGCAGGAATGGGAGCGCCCACACAGGTGCTGCAGATTGTCCTGAAAAGATGTGAAGAAAGCAAATC
  TCGAAGCAAGGATGAATATCAAGCTGCAGTAGAAAGATTGGTCATGGCTGCTCGAATATCGGACCCCAAG
  CTTTTCATTAAGCACATGACTGTCAATATTAACAAGGAGCAGGTGTACAGTCTGGAGCACTGTTCTGCTT
  TGAAATGGCTGAAAGAGAATATGAAGTGGGCTGGAAAGGTTTGGCTTTTCACTAACCATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199736
• Insert Size: 4962 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199736.1, NP_001186665.1
• RefSeq Size: 4969 bp
• RefSeq ORF: 4962 bp
• Locus ID: 671232
• UniProt ID: E5FYH1
• Cytogenetics: 9
• Gene Summary: Important for normal spermatogenesis and male fertility. Specifically required for progression to the post-meiotic stages of spermatocyte development. Seems to be necessary for normal expression levels of a number of testis-expressed gene transcripts, although its role in this process is unclear.[UniProtKB/Swiss-Prot Function]