Adgrb2 (NM_001290715) Mouse Untagged Clone

CAT#: MC229629

Bai2 (untagged) - Mouse brain-specific angiogenesis inhibitor 2 (Bai2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrb2
• Synonyms: Bai2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229629 representing NM_001290715
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGATTCTGTCCCTGCGCCTGGCCACGGCCTTCGACCCTG
  CCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGCGTGCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCC
  CACCATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCAGACCCCACCAAGTACTCCCTCTACCTGCGC
  TTCAACCGGCAGGAGCAGGTTTGCACACACTTTGCCCCGCGCCTGCTGCCCCTGGACCACTACCTGGTCA
  ACTTTACCTGCCTGCGGCCTGGTCCAGAGGAAGCCACAGCCCGGGCTGAGTCGGAGGTGGGACGGCCAGA
  GGAGGAGGAGGAGGAGGCGGCGGCAGCAGCATCAGGGTTGGAGTTGTGTGGTGGCTCAGGCCCCTTTACC
  TTTCTGCACTTCGACAAGAACTTTGTGCAGCTGTGCCTGTCGGCTGAGCCCTCTGAGGCCCCTCGTCTGC
  TAGCGCCTGCTGCCCTGGCCTTCCGTTTTGTGGAGGTCTTGCTGATCAACAACAACAACTCCAGCCAGTT
  CACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGGGCTGCAGGCAGGGCTTGTGGCTTTGCA
  CAGCCAGGGTGTAGTTGTCCTGGGGAGGCAGGGGCCAACCCCGCCACCACCACATCTCCGGGGCCTCCGG
  TTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGCCCCTCCTGCTGAGGCCGACTTGCACTC
  GGGGAGCAGCAATGACCTGTTCACCACCGAGATGAGATATGGTGAGGAGCCGGAAGAGGAACCGAAGGTG
  AAAACCCAGTGGCCAAGGTCTGCAGATGAGCCTGGGCTATACATGGCGCAGACAGGCGACCCAGCAGCTG
  AGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTG
  CGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTTCGGGAGACCCGGCCTTGCAACAATTCAGCC
  ACCTGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGAGCCTGTGCTCCCGCAGCTGCGGGCGGG
  GGTCTCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGGCGGCAAGGCCTGCGAGGGTCCCGAGCT
  GCAGACTAAACTCTGCAGTATGGCCGCCTGCCCGGTGGAAGGCCAGTGGCTAGAATGGGGTCCCTGGGGC
  CCATGCTCATCATCTTGTGCCAATGGGACCCAGCAGCGCAGCCGGAAATGCAGTGTGGCGGGTCCAGCCT
  GGGCCACGTGCGCAGGTGCCCTCACGGATACCCGTGAGTGCAGCAATCTCGATTGCCCGGCCACTGACGG
  CAAGTGGGGGCCGTGGAACGCGTGGAGCCTGTGCTCCAAGACGTGTGACACGGGCTGGCAACGCCGCTTC
  CGCATGTGCCAGGCTTCTGGCACACAGGGCTACCCTTGCGAGGGCACAGGAGAGGAGGTGAAACCCTGCA
  GTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGAGTACGTGATGTTGATGACATGGAAGAG
  GGCGGCAGCTGGCGAGATCATTTACAACAAGTGTCCCCCTAATGCCTCGGGTTCTGCTAGCCGCCGCTGT
  CTCCTCAGTGCCCAGGGCGTAGCATACTGGGGACTGCCCAGCTTTGCTCGTTGCATATCCCATGAATACC
  GCTACCTGTACCTGTCACTTCGGGAACACCTGGCTAAGGGCCAGCGCATGCTGGCAGGTGAGGGCATGTC
  ACAGGTGGTGCGGAGCCTGCAGGAGCTACTGGCACGGCGCACTTACTACAGCGGGGACCTGCTCTTCTCT
  GTGGACATCCTAAGGAACGTCACTGACACCTTCAAGAGGGCCACCTATGTCCCTTCCGCCGATGACGTGC
  AGCGTTTCTTCCAGGTGGTGAGCTTCATGGTGGATTCAGAAAACAAGGACAAATGGGATGATGCTCAGCA
  GGTGTCACCGGGCTCTGTGCACCTGCTGCGTGTTGTGGAAGATTTCATTCACCTCGTGGGCGACGCTCTC
  AAGGCCTTCCAGAGCTCTCTCATTGTCACGGACAATCTGGTGATCAGCATTCAGAGAGAGCCTATCTCCG
  CCGTGTCCAGTGACATCACGTTTCCCATGCGGGGCCGCAGGGGCATGAAGGACTGGGTGCGACACTCAGA
  GGATCGTCTCTTTCTACCCAAGGAGGTGCTCAGCCTGTCCTCCCCAGGAAAGCCAGCCACACCTGGGGCA
  GCCACAGCAGGCAGCCCGGGCAGGGGGAGGGGCCCAGGAACGGTGCCCCCTGGCCCAGGCCACGCCCACC
  AGCGCCTTCTCCCAGCTGACCCCGAAGAGTCCTCCTCCTACTTTGTGATCGGTGCTGTGCTCTACCGCAC
  CCTTGGCCTCATCCTGCCGCCCCCCAGGCCTCCACTTGCTGTCACCTCCCGGGTGATGACAGTGACTGTG
  CGTCCCCCCACCCAGCCTCCAGCTGAGCCCCTCATTACAGTGGAACTCTCGTACATCATCAATGGCACCA
  CCGATCCCCACTGTGCCAGCTGGGACTACTCCAGAGCAGATACCAACTCGGGGGACTGGAACACTGAGAG
  CTGCCAGACCTTGGAGACCCAGGCGGCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCCGTC
  CTGGCCCAGCCACCCAAGGACCTGACCCTGGAGCTGGCAGGTGCTCCCTCTGTCCCCCTGGTGATCGGCT
  GTGCAGTGTCCTGCATGGCTCTGCTCACCCTGCTGGCCATCTATGCAGCCTTCTGGAGGTTTATAAAATC
  AGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCCTGGCTTCCAACATTCTGATCCTGGTGGGC
  CAGTCCCGGGTGCTGAGCAAGGGCGTATGCACCATGACGGCTGCCTTCCTACACTTCTTCTTTCTGTCCT
  CCTTTTGCTGGGTGCTTACAGAGGCCTGGCAATCCTATCTGGCTGTCATCGGGCGGATGCGCACCCGCCT
  GGTTCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTACCTGCCCTGGTGGTGGCTGTGTCTGTTGGCTTT
  ACTCGCACCAAAGGATATGGTACATCCAGCTACTGCTGGCTGTCCCTAGAGGGCGGCCTGCTCTATGCCT
  TCGTGGGTCCAGCAGCAGTCATTGTCCTGGTGAACATGCTCATCGGGATTATCGTCTTCAACAAGCTCAT
  GGCTCGCGATGGCGTCTCAGACAAATCTAAGAAGCAGAGGGCTGGGTCGGAGCGGTGCCCCTGGGCCAGC
  CTGCTCCTTCCCTGCTCAGCGTGTGGAGCGGTCCCCAGCCCCCTGCTCAGCTCAGCCTCGGCCAGGAATG
  CCATGGCTTCACTCTGGAGTTCCTGCGTGGTACTGCCTCTCCTGGCGCTTACCTGGATGTCTGCCGTCCT
  GGCCATGACAGATCGCCGCTCCGTCCTCTTCCAGGCACTCTTTGCCGTTTTCAACTCTGCACAAGGCTTT
  GTCATCACCGCTGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTAAAGTGTCAGATGGGTGTGT
  GTCGGGCTGATGAGAGTGAAGACTCCCCAGACTCGTGCAAGAACGGGCAGCTGCAGATCCTGTCAGACTT
  TGAAAAGGACGTGGATCTGGCTTGTCAGACAGTTCTGTTCAAGGAGGTCAACACCTGCAACCCGTCCACC
  ATTACCGGCACTCTGTCCCGCCTGTCTCTGGATGAGGATGAGGAGCCCAAGTCCTGTCTCGTGGGTCCTG
  AGGGTGGCCTCAGCTTCTCACCACTGCCTGGGAACATCCTGGTACCCATGGCAGCCTCACCAGGTCTAGG
  GGAGCCACCACCACCCCAGGAGACCAACCCTGTGTACATGTGTGGGGAGGGTGGCCTGCGGCAGTTGGAC
  CTTACATGGATACGGCAGAGTGAACCAGGCTCTGAGGGGGACTACATGGTTCTGCCCCGGCGGACTTTGA
  GCCTGCAGCCTGGTGGTGGGGGTACAGCGGGTGAGGAGGCCCCAAGGGCCCGGCCTGAGGGGACCCCCCG
  GCGGGCTGCCAAAACGGTAGCCCACACTGAAGGCTACCCCAGCTTCCTGTCTGTGGAGCACTCGGGTCTA
  GGGCTGGGCCCTGCCTATGGGTCTCTCCAGAACCCGTATGGAATGACCTTCCAACCACCACCACCAACAC
  CCAGCGCCCGCCAAGTACCAGAGCCAGGAGAACGTAGCCGGACCATGCCCCGTACAGTGCCTGGTTCCAC
  CATGAAGCTGGGCTCCCTTGAGCGAAAGAAGCTTCGGTATTCGGACTTGGACTTTGAGAAGGTGATGCAC
  ACTCGGAAACGGCACTCGGAACTCTACCACGAACTCAACCAGAAGTTCCACACTTTCGACCGCTACCGTA
  GCCAGTCCTCAGCCAAGGAGAAACCCAGCCCCCCCGGGGGACGCCCTGGCTTGTCCCAGCACAGGAGGCA
  TCAAAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCACTGCCCCCCAAGCCCCGAGAACGGCTGGCC
  CTGCACCGGACAGCAGCCTGGGAGCCCACAGAACCGCCAGACGGCGACTTCCAGACAGAGGTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001290715
• Insert Size: 4686 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290715.1, NP_001277644.1
• RefSeq Size: 5011 bp
• RefSeq ORF: 4686 bp
• Locus ID: 230775
• UniProt ID: Q8CGM1
• Cytogenetics: 4 D2.2
• Gene Summary: Orphan G-protein coupled receptor involved in cell adhesion and probably in cell-cell interactions. Activates NFAT-signaling pathway, a transcription factor, via the G-protein GNAZ. Involved in angiogenesis inhibition (PubMed:12218411).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1.