Cux1 (NM_001291234) Mouse Untagged Clone

CAT#: MC229612

Cux1 (untagged) - Mouse cut-like homeobox 1 (Cux1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cux1
• Synonyms: CDP; Cutl1; Cux; Cux-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229612 representing NM_001291234
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTGCAGAGAGAAC
  TTGATGCCACCGCAACAGTATTGGCAAACAGGCAAGATGAGAGCGAACAGTCCAGAAAGCGGCTCATTGA
  GCAGAGCCGAGAATTCAAGAAGAACACTCCAGAGGATTTACGCAAGCAGGTAGCACCACTGCTAAAGAGC
  TTCCAAGGGGAGATTGATGCACTGAGTAAAAGAAGCAAAGAAGCAGAGGCAGCCTTCTTGACTGTGTACA
  AGAGACTAATTGATGTTCCAGATCCGGTACCAGCCCTGGACGTCGGGCAACAGCTGGAAATAAAAGTGCA
  GCGTCTACACGACATTGAAACAGAGAACCAGAAACTTAGGGAAACACTAGAAGAGTACAACAAGGAGTTT
  GCTGAAGTGAAAAATCAAGAGGTTACGATAAAAGCACTTAAGGAGAAAATCCGAGAATACGAGCAGACCC
  TGAAGAGTCAGGCCGAGACCATTGCTCTGGAGAAAGAGCAGAAGCTACAAAATGATTTTGCAGAGAAGGA
  GAGAAAGCTGCAAGAGACACAGATGTCCACCACCTCAAAACTGGAGGAAGCTGAGCACAAACTCCAGACT
  CTGCAAACAGCCCTGGAAAAAACTCGAACAGAATTATTTGACCTGAAAACCAAATATGATGAAGAAACTA
  CTGCAAAGGCCGATGAGATCGAGATGATCATGACCGACCTTGAACGAGCCAACCAGAGGGCAGAGGTGGC
  ACAGAGAGAAGCAGAGACTTTAAGGGAACAGCTCTCATCGGCCAACCACTCTCTCCAACTGGCCTCGCAG
  ATCCAGAAGGCTCCAGATGTGGCCATAGAGGTGCTGACCCGATCCAGCCTAGAAGTAGAGTTGGCTGCCA
  AAGAGCGGGAGATCGCCCAGCTGGTGGAAGATGTGCAGCGACTCCAGGCCAGCCTCACCAAGCTACGTGA
  GAATTCCGCCAGCCAGATCTCACAGCTGGAGCAGCAACTGAATGCCAAGAATAGCACACTCAAACAACTG
  GAAGAAAAACTCAAAGGCCAGGCTGACTATGAAGAAGTGAAGAAAGAGCTGAACACCCTGAAGTCCATGG
  AGTTTGCACCATCGGAGGGAGCAGGGACACAGGACTCTACCAAGCCCCTGGAGGTTTTACTCCTGGAGAA
  GAACCGCTCGCTGCAGTCCGAGAATGCCACGCTGCGCATCTCCAACAGTGACCTGAGCGGGTCAGCCAGG
  AGAAAAGGGAGAGACCAGCCTGAGAGTCGGCGCCCGGGACCCTTGCCGGCCTCCCCTCCTCCTCAGTTGC
  CCCGCAACACGGGGGAACAGGTTTCCAATACTAACGGTACACACCACTTCTCACCAGCGGGGTTAAGTCA
  AGACTTTTTCAGCTCAAACCTGGCCAGCCCCAGCCTACCCCTGGCTTCTACAGGAAAGTTTGCACTAAAC
  TCTCTTCTCCAGCGACAGCTAATGCAGTCCTTCTACTCCAAGGCCATGCAGGAAGCCGGAAGCACAAGCA
  CCATTTTTTCAACAGGTCCTTACAGCACAAACTCCATATCTTCCCCAAGTCCATTACAACAAAGCCCAGA
  TGTAAATGGCATGGCCCCATCTCCCAGCCAATCAGAAAGTGCTGGGAGCATCTCAGAGGGCGAGGAAATT
  GATACCGCAGAGATCGCCCGGCAGGTCAAAGAGCAGCTCATCAAGCACAACATTGGCCAGCGCATCTTCG
  GACATTACGTCCTGGGACTGTCGCAAGGCTCTGTGAGCGAGATCCTGGCCCGGCCCAAGCCCTGGAACAA
  GCTGACTGTCCGTGGCAAGGAGCCATTCCACAAGATGAAGCAGTTCCTGTCTGATGAGCAGAACATCCTG
  GCACTCCGTAGCATCCAAGGCAGACAGAGAGGTAACATCACTACCCGGATCCGAGCATCTGAGACTGGTT
  CTGATGAAGCCATCAAGTCCATCCTGGAACAAGCCAAGAGGGAACTACAAGTGCAGAAAACCGCTGAGCC
  AGTCCAGACATCTTCCACGTCCAGCAGTGGGAACTCTGACGATGCCATCCGCTCCATCCTACAGCAGGCC
  CGCCGGGAAATGGAGGCCCAGCAGGCTGCCCTTGACCCTGCCTTAAAGCCAGCACCACTGTCCCAGCCCG
  ACCTCACCATCCTCACCCCCAAGCACCTGTCTGCCTCACCCATGTCTACTGTGTCCACTTACCCGCCTCT
  TGCTATCTCCCTGAAGAAAACCCCTGCAGCACCTGAGACCAGTACCGCAGCCTTGCCCAGTGCCCCAGCT
  CTCAAAAAAGAGGCTCAGGATGTACCCACACTGGACCCACCAGGATCAGCTGATGCTGCCCAGGGAGTGC
  TGAGGCCGATGAAGAGTGAGCTAGTCCGTGGCAGCACCTGGAAGGATCCCTGGTGGAGCCCTATACAGCC
  TGAAAGAAGAAACCTCACTTCTTCCGAAGAGACCAAGGCCGATGAAACAACTGCAAGCGGGAAAGAAAGG
  GCAGGCAGCAGCCAGCCTCGGGCTGAGCGCAGCCAGCTTCAGGGACCCTCAGCATCCGCAGAGTACTGGA
  AAGAGTGGCCAAGCGCTGAGTCCCCATACTCCCAGAGCTCAGAGCTGAGCCTCACTGGAGCCAGCCGCAG
  TGAGACACCCCAGAATAGCCCTCTGCCCTCTTCCCCAATTGTGCCCATGGCGAAGCCAGCCAAGCCTTCA
  GTGCCCCCGCTGACTCCTGAGCAGTACGAGGTCTACATGTATCAGGAAGTGGACACTATCGAGCTCACTC
  GGCAGGTCAAAGAGAAGCTGGCCAAGAACGGCATTTGCCAGAGGATCTTTGGGGAGAAGGTACTGGGCCT
  GTCCCAGGGCAGTGTCAGCGACATGCTTTCAAGGCCAAAACCATGGAGCAAACTGACCCAGAAAGGCCGA
  GAACCCTTCATCCGGATGCAGCTCTGGCTGAATGGAGAGCTGGGCCAAGGTGTTCTGCCAGTGCAAGGGC
  AACAGCAAGGGCCAGTCCTCCACTCCGTGGCGTCTCTGCAGGACCCCCTCCAGCAGGGCTGTGTGAGCTC
  AGAAAGCACTCCAAAGACCTCTGCCAGCTGCAGCCCTGCCCCTGAGTCCCCAATGAGTTCCAGCGAATCT
  GTGAAGAGTCTCACCGAGCTGGTCCAGCAGCCCTGTCCCGCCATTGAGACCAGTAAAGAAGGCAAACCAC
  CAGAGCCCAGCGACCCGCCTGCCTCAGACTCCCAGCCCACAACACCGCTGCCTCTCTCTGGACACTCAGC
  CCTCAGCATCCAAGAATTAGTAGCCATGTCTCCTGAGCTGGACACGTATGGCATAACCAAGAGGGTCAAA
  GAGGTGCTGACAGACAACAACCTCGGTCAGCGCTTATTTGGGGAGACCATTTTGGGGCTCACCCAGGGTT
  CTGTCTCCGACCTCCTTGCCCGCCCAAAGCCTTGGCATAAGCTTAGTCTGAAAGGACGGGAACCCTTTGT
  CCGCATGCAGCTATGGCTGAACGACCCCAACAATGTGGAGAAGCTGATGGATATGAAGCGGATGGAGAAG
  AAAGCCTACATGAAGAGGCGACACAGCTCTGTCAGTGACAGTCAGCCTTGTGAGCCCCCCTCTGTGGGTA
  TTGACTATAGCCAAGGCGCCAGCCCCCAGCCACAACACCAGCTGAAGAAACCTCGAGTGGTGCTGGCTCC
  AGAGGAGAAGGAAGCGCTGAAGCGAGCATATCAGCAGAAGCCATACCCATCACCAAAAACCATCGAGGAA
  CTTGCCACACAACTCAACCTGAAGACCAGCACCGTCATCAACTGGTTCCATAATTACAGGTCTCGGATCC
  GCAGAGAACTGTTCATTGAGGAGATTCAAGCTGGAAGCCAGGGCCAGGCTGGTGCCAGCGACTCACCTTC
  AGCTCGAAGTAGCCGCGCTGCACCCAGCTCGGAAGGTGACAGCTGTGACGGCGTGGAAGCCACCGACGCC
  GAGGAGCCAGGTGGCAACATAGTGGCCACCAAGTCTCAGGGAGGGCTGGCAGAGGTGGCCGCAGCCCCGG
  CAGACCGGGAAGAAGCGACGCAGCCGGCGGAGAAGGCGAAGGCACAGCCACTATGCTCGGGGACCCCAGG
  ACAGGACGACGGTGAAGACGCAAGCCGGCCTAGGCCACTTCCCGAGGGCCTCGCCGACGCCCCAGCGCCT
  GTGCCAAGCCTCGCCGCACCCGCGGCCGGAGAGGACGCCGCTACCTCAGCCACTGCCCCGGCCACGGCCA
  CCGAGGCCCCCGGGGCGGCCAGGGCGGGCCCTGCCGAGAGGAGTTCTGCGTTGCCAAGCACCAGCGCGCC
  TGCCAACGCGCCTGCGCGAAGGCCTAGCTCACTGCAGAGCCTCTTCGGTCTGCCCGAGGCCGCGGGCGCC
  CGGGACAACCTGGTGCGGAAGAAGAAGGCTGCGAACTTGAACAGCATCATCCACCGCCTGGAGAAGGCTG
  CCAGCCGGGAGGAGCCCATCGAATGGGAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291234
• Insert Size: 4515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291234.1, NP_001278163.1
• RefSeq Size: 13146 bp
• RefSeq ORF: 4515 bp
• Locus ID: 13047
• UniProt ID: P53564
• Cytogenetics: 5 75.96 cM
• Gene Summary: Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III (PubMed:20510857). Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and 5' coding region, and it also uses an alternate in-frame splice site and lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.