Col17a1 (NM_001290825) Mouse Untagged Clone

CAT#: MC229600

Col17a1 (untagged) - Mouse collagen, type XVII, alpha 1 (Col17a1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col17a1
• Synonyms: BP180; Bpag; Bpag2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229600 representing NM_001290825
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGACCAAGAAAAGCAAGCGAGATGGCACTGAAGTCACCGAGAGAATTGTCACGGAAACAGTAA
  CGACAAGACTTACATCCCTACCGCCAAAAGGGAGCACCAGCAATGGATATGCTAAGACAGGCTCTCTGGG
  TGGAGGAAGTCGGTTGGAGAAGCAGAGCTTGACCCATGGCAGCAGCGGCTACATCAACTCAAGTGGAAGC
  ATCCGAGGCAACGCTTCCACCTCCAGTTACAGGAGAACGCACTCACCGGCCTCTACCCTGCCCAACTCTC
  CCGGCTCCACCTTTGAAAGGAAAGCTCACATGACCCGCCATGGAACTTATGAAGGGAGCTCTAGCGGCAA
  CTCCTCCCCTGAGTACCCACGAAAGGAACTCGCCTCTTCTTCAACCAGAGGACGAAGCCAAACACGAGAG
  AGCGAAATTCGAGTTCGGTTGCAGAGCGCGTCGCCATCCACCAGATGGACAGAACTGGATGAGGTGAAGC
  GTTTGCTTAAGGGGAGCCGCTCTGCAAGTGCAAGTCCCACCAGGAACACCTCCAACACACTCCCCATCCC
  CAAGAAAGGCACCGTAGAGACCAAAACAGTGACAGCGAGCTCCCACTCAGTGTCCGGAACCTATGATTCA
  GCAATACTGGACACCAACTTTCCGCCCCACATGTGGTCCTCCACCTTGCCTGCAGGGTCCTCCCTGGGGA
  CCTATCAGAACAACATAACAGCCCAGAGCACATCCCTCCTCAACACCAATGCCTACTCAACGGGATCAGT
  CTTTGGAGTGCCAAATAACATGGCGTCCTGCTCTCCCACCCTGCACCCCGGACTCAGCAGCTGCTCCTCA
  GTGTTTGGCATGCAGAACAATCTGGCCCCCAGCTCTTCTGTCCTCTCCCATGGCACAACCACCGCTTCCA
  CAGCATACGGAGCGAAGAAAAACGTGCCCCAGCCTCCCACTGTCACCAGCACCGGCGTGTCCACCTCTGC
  CACCTGCACCACCAGTGTCCAGAGTGACGACCTCTTGCATAAAGACTGCAAGTTCCTGATTCTGGAGAAA
  GACAACACACCAGCTAAGAAAGAGATGGAACTCTTGATCATGACCAAGGACAGTGGGAAGGTCTTCACTG
  CCTCCCCTGCCACCATCTCTTCAACTTCCTTTTCAGAAGACACCCTGAAAAAGGAGAAGCAGGCTGCATA
  TGCCGCTGACACCTGCCTGAAGGCAGATGTGAATGGAGACCTAAATACCGTGTCCACAAAGAGCAAGATG
  ACCTCGGCAGAAAACCATGGCTACGACCGAGGTGGCGGTGGTGGCAGAGGCAAAGGCGGAGGTGCTGGTG
  GTGGCGGTGGTGGCGGTGGCGCCAGTGGCGGTGGAGGAGCATGGGGGGCTGCACCAGCCTGGTGCCCCTG
  CGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACCTGGCTGCTGCTGCTGGGTCTG
  CTCTTCGGCCTCATTGCTCTGGCGGAGGAGGTAAGAAAGCTGAAGGCCCGCGTGGAGGAGCTGGAAAAGA
  CCAAGGTGCTATATCATGACGTCCAGATGGACAAAAGCAACAGGGACCGCCTCCAGGCCGAGGCACCCAG
  CCTGGGACCTGGATTAGGCAAGGCTGAGCTGGACGGCTACAGCCAGGAGGCCATCTGGCTGTTTGTAAGG
  AACAAGCTGATGACCGAGCAGGAGAACGGGAATCTCAGAGGAAGTCCTGGTCCAAAAGGTGACATGGGGA
  GTCAAGGACCTAAAGGAGACCGAGGCCTTCCTGGGACCCCAGGTATCCCTGGGCCCCTGGGCCACCCTGG
  CCCGGAAGGACCAAAGGGACAAAAAGGCAGCATTGGAGATCCTGGCATGGAAGGACCCATAGGCCAGAGA
  GGACTAGCAGGCCCCATGGGACCTCGTGGTGAACCCGGGCCTCCTGGGTCTGGAGAGAAAGGAGACAGAG
  GGATTGCTGGTGAACAAGGTCCTCAGGGCCTTCCTGGTGTCCCAGGTCCTCCGGGTCTCAGAGGTCACAG
  CGGCTCTCCTGGTCCCCAAGGCCCCCCAGGTGCTGTGGGTCCCCAAGGGCTCCGAGGTGACGTGGGACTT
  CCTGGTGTCAAAGGTGACAAAGGACTCATGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAGGGAC
  CCAGAGGCCTCACAGGGGAGCCTGGCATTCGAGGTTTGCCTGGAGCTGTGGGTGAACCCGGAGCCAAAGG
  CGCAATGGGTCCGGCTGGCGCTGATGGACAGCAAGGTTCCAGAGGTGAACAAGGCTTGACAGGGATGCCT
  GGAACCCGGGGCCCCCCAGGACCCGCTGGAGACCCAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTC
  AGGGACTTCCTGGTAGCCCTGGCCGACCAGGGACTAAAGGCGAACCCGGCGCTCCCGGCAGAGTCATGAC
  TTCAGAGGGATCATCAACAATCACTGTGCCCGGACCTCCCGGACCTCCTGGTGCCATGGGTCCCCCAGGA
  CCTCCAGGGACGCCAGGTCCAGCTGGCCCTGCTGGTCTCCCAGGACAACAAGGCCCACGAGGGGAGCCAG
  GACTTGCTGGTGACTCATTCCTAAGCAGTGGCAGCTCCATCTCTGAGGTCCTCTCTGCCCAAGGTGTTGA
  CTTACGAGGTCCCCCTGGCCCACCTGGCCCACGAGGGCCACCAGGGCCTTCCATCCCAGGCCCGCCAGGA
  CCCAGAGGTCCACCAGGGGAAGGCGTACCAGGCCCACCCGGGCCACCAGGATCCTTCCTGACTGACTCAG
  AAACCTTCTTCACTGGCCCTCCAGGTCCACCCGGCCCCCCAGGTCCCAAGGGAGACCAAGGTGATCCTGG
  TGTCCCAGGTACTCCTGGCATCTCTGGTGGTCTTTCTCATGGGGCGTCATCAAGCACTCTGTACATGCAG
  GGCCCCCCTGGCCCTCCAGGGCCCCCTGGGCCTCCGGGCTCCCTCAGCAGCTCCGGCCAGGATATCCAGC
  ACTACATCGCTGAATACATGCAAAGTGACAACATCAGGACTTATCTCTCAGGGGTTCAGGGTCCCCCAGG
  CCCACCAGGTCCCCCAGGGCCTGTCATCACCATCACAGGAGAGACTTTCGACTACTCCCAGCTGGCAAGC
  CAGGTCGTAAGCTACTTGCGGTCATCAGGCTACGGTGCTGGCTTGTCCTCTGCCTCCTCCTCAGAAGATA
  TCTTGGCCATGCTGCGACGGAACGATGTGTGGCAGTACTTACGTCAGAATCTGGTGGGTCCTCCCGGTCC
  CCCAGGGCCACCAGGCGTCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGGAGAGCTGAGCAGACAC
  ATTCTCAACTATATGTCAAGTTCTGGGATCAGCTTTGGGCATCCTGGCCCACCGGGACCCCCTGGCCTGC
  CAGGAACATCCTACGAGGAGCTACTGACCATGCTCCGAGGGTCTGACTACAGAAACATCATTGGACCTCC
  AGGGCCCCCAGGGCCACCCGGGATGCCAGGCAATGCATGGTCCAGCATCAGTGTGGAGGACCTCTCATCA
  TACTTGCACACCGCTGGTTTGTCCTCCATCCCAGGCCCTCCAGGCCCACCAGGGCCCCCAGGTCCTCGAG
  GGCCCCCAGGTGTCTCAGCAGCTCTGTCCACCTATGCAGCTGAGAACAGCGACAACTTCCGCAGCGAGCT
  GATTAGCTACCTCACAAGTCCTGATGTTCGCAGCTTCATCGTTGGCCCTCCAGGCCCTCCGGGGCCACAG
  GGACCACCTGGAGATGGTCACTTAAGGGAGAACTACAACTGGAGCAGCAACTCCTCAGCCAGAAGGGGCA
  CTTCCTACAGCTCTTCCACGGGCACAGGGGGAACCAACGGAGGCTCCCTGGGTGAAGGTGGAGCCTATGG
  TGCAGGAGATGGGGGCCCCTATGGTACCGACATCGGCCCAGGCGGAGGCTATGGGGCAGCTGCAGGTGGT
  GGTATATATGGCACCAATGGTGACTCGTTCCGGGATGGCTTCACTGGAGACCTAGATTACAACAAGCTGG
  CAGTGCGGGTGTCGGAGAGCATGCAGCGTCAGGGTCTGCTACAAGGGATGGCCTACACTGTCCAGGGCCC
  ACCTGGGCCCCAAGGCCCTCCTGGCATCAGCAGGGTCTTCTCAGCCTACAGCAACGTGACACAAGACCTC
  ATGGACTTCTTCCAAACCTACGGCACTATCCCGGGACCACCTGGGCAGAAGGGAGATGTGGGAACCCCAG
  GCCCCAAAGGTGACAGGGGCCCTGCTGGACCACGAGGTCCTCCAGGGCCACCCGGCCCCAGAGGGAACAA
  AGGAGAGAAAGGAGACAAAGGTGACCAAGTCTATACTGGGAGAAGGAAGAGAAGTATTGCCATCAAGCCG
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290825
• Insert Size: 4413 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290825.1, NP_001277754.1
• RefSeq Size: 5539 bp
• RefSeq ORF: 4413 bp
• Locus ID: 12821
• UniProt ID: Q07563
• Cytogenetics: 19 40.07 cM
• Gene Summary: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.