Eif4g3 (NM_001256198) Mouse Untagged Clone

CAT#: MC229598

Eif4g3 (untagged) - Mouse eukaryotic translation initiation factor 4 gamma, 3 (Eif4g3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif4g3
• Synonyms: 1500002J22Rik; 4930523M17Rik; eIF4GII; G1-419-52; repro8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229598 representing NM_001256198
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCACAACCTCAGGCCCGGTCTCCGGGAGGATTCAGACCCATCCAGTTTTTCCAGAGGCCTCAAA
  TCCAGCCTCCCCGAGCTGCCATCCCAAACAGCAGTCCTTCCATCCGTCCCGGTGTGCAGACCCCCACCGC
  CGTGTATCAGGCCAACCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCCGTGACCCAGGGG
  CATCAGTACTGCATACCACAGTACCGCCACAGCGGCCCTCCGTACGTGGGGCCCCCACAGCAGTATCCAG
  TTCAGCCGCCTGGGCCGGGGCCTTTTTATCCTGGACCAGGACCTGGGGACTTCGCTAATGCTTATGGAAC
  GCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAGCAGCCCCCT
  CCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGAGATCCAAATCAGGGAGGTAAAGACATAACAGAGG
  AGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGAAGACCCGCATCCACTCCCACTCC
  TCCTCAGCAGCTGCCCAGCCAGGTCCCTGAGCACAGCCCTGTGGTATATGGGACTGTGGAGAGCGCTCAT
  CTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAAGAAAAGCCAAAACCAGATCCAG
  TGTTCCAGTCTCCTTCGACGGTCCTTAGGCTCGTACTCAGTGGGGAGAAGAAAGAGCAAGCAGGCCAGAT
  GCCTGAGACTGCTGCAGGAGAGCCCACCCCAGAGCCTCCTCGGACTTCATCACCCACCAGCCTTCCTCCA
  CTTGCCCGGAGTTCACTTCCTTCCCCCATGTCCGCTGCCCTTAGCAGCCAGCCACTGTTCACTGCTGAGG
  ACAAGTGTGAACTTCCTTCCTCCAAAGAAGAAGATGCACCTCCTGTCCCCAGCCCCACGTCTTGCACAGC
  AGCCTCAGGCCCTTCACTAACAGATAACAGTGATATATGCAAGAAACCCTGTAGCGTAGCGCCTCATGAT
  AGTCAGCTAATTTCTAGTACTATCCTAATTAATGAAATGAATGGAGTTGGAGAAAAATTATCAGCCAAGG
  AGAACACTGTGGGCATGCTGAGACAGGAAGTGCTGCCATTGACTCTCGAGTTAGAGATTCTGGAACATCC
  ACAGGAAGAATTGAAAGTGGAATGTACGCCGACTCCCATTGCCCCGTCCATGCTGCCTGCCTTCTCCCCA
  GCTCCTCCGACTCCCCCCACTTCTCCTCCTTGTCCTCCGGTTGTCCTCTCTGCCGCCATTGCTCGTTCTC
  CCGCTGTTGCCACTGAGGTCCAGAGAGTCGCCGACGAGGGAGAAAGCCTGAGAACTTGCCTTAGCAAGGA
  TGCAAAGGAGATGCAGGACAAAGCAGAGTCAGAATCAGATGGGCAAGCAGAAGAGACTGCGGATCCCCAG
  AGCCTACATTCAGGAAGGAGCCCCGCCCCAGTGCAAACAGCTACCACTGCGCCAAAGTCCTGGAAGAAAA
  CAAAAGAGCAGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAGCCTAAAGCCGAAGAAGAGCT
  TGCAGTTGACAGTGTCCTTGAGCCTGAGCAAGAGAAGATGAGCCAAGGGTTTCCGTCTGAGAGAGACCCC
  TCTGCCCTGAAGAGAGGGAAAGCTGAGGAAGGAAACGGAGAAGAGGCTGAGCCTGTGCGAAATGGCGCTG
  AGAGTGCTTCTGAGGGGGAAGGGGGAGATGGCAACTCAGGCTCTGCTGACAGCTCTGCTGATGGGCTCAC
  CTTCCCGTTTAAAGCAGAGTCCTGGAAGCCTGCAGATACAGAAGGCAAGAAGCAGTATGACCGGGAGTTC
  CTACTGGACATCCAGTTTATGCCCGCCTGTATCCAAAAACCAGAAGGCCTGCCTCCCATCAGCGACGTGG
  TCCTTGACAAGATCAACCAGCCCAGATTGTCAATGCGGACCCTGGATCCTCGCATTTTGCCTCGAGGACC
  TGACTTCACCCCAGCCTTTGCAGACTTTCCAAGGCAAACGCCTGGTGGAAGAGGTGTGCCTCTGTTGAAT
  GTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGAAAGATAATCACTGTCTCCGTAAAAG
  AAGATGTGCACTTGAGAAAGGCGGAGAATGCCTGGAAGCCGAGCCAGAAACGGGACAGCCACGCCGATGA
  CCCCGAAAGCATTAAAACTCAGGAACTTTTCAGAAAAGTTCGAAGTATCTTGAATAAACTGACACCACAG
  ATGTTCAACCAGCTGATGAAGCAAGTGTCGGCTCTTACGGTGGACACGGAGGAGCGGCTAAAAGGGGTCA
  TCGACCTTGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCGTACGCAAACATGTGTCGGTG
  CCTAGTAACGCTGAAGGTGCCCATGGCAGACAAGCCTGGCAACACAGTGAATTTCCGGAAGCTGCTGCTT
  AACCGCTGCCAGAAGGAGTTCGAGAAAGACAAAGCAGATGATGACGTCTTTGAGAAGAAGCAGAAAGAAC
  TGGAAGCTGCCAGCGCTCCAGAGGAGAGGACAAGGCTTCACGACGAACTGGAAGAAGCCAAGGACAAAGC
  CCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAACTCTTTAAACTGAAAATGCTGACGGAGGCC
  ATCATGCATGACTGTGTGGTGAAGCTCCTGAAGAACCACGATGAAGAGTCCCTGGAGTGCCTGTGTCGCC
  TGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCACGCATGGACCAGTACTTTAACCA
  GATGGAGAAAATCGTCAAAGAAAGAAAAACTTCCTCCAGAATTCGGTTCATGCTTCAGGATGTAATCGAC
  CTCAGACTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAAACTATCGAGCAGATTCACAAAG
  AGGCCAAAATAGAAGAACAAGAGGAGCAGAGGAAAGTCCAGCAGCTGATGACCAAGGAGAAGAGGAGACC
  AGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCAAGAACAGTCGTGTACTGGAC
  CCCTCCAAATTTTTGAAAATTACTAAACCCACCATTGATGAGAAGATTCAGCTGGTTCCTAAGGCACAGC
  TGGGCAGCTGGGGCAAAGGCAGCAGCGGGGGAGCAAAGGCAAGTGAGTCCGATGCCTTACGGTCAAGTGC
  TTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCACCTTCAGGGTCCCCGTCGGCCACACCCTTA
  GAGTTTGATTCCCGAAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGAGAAGAGCGACAAGCCAATTC
  CAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGACCTGCTGGACAACCAGTCCCA
  GGAAGAGCAGCGCAGAGAGATGTTGGAGACCGTGAAACAGCTGACAGGAGGCCTGGATGCTGAGCGGGCC
  AGCACTGAGGCCGACCGAAGCAAAACGAGGGAGCTAGCAAAATCGGAAATGTGTGCAGTGCCAGCCCCTG
  ACAAGCCTGCACTGTCAGAAGAGGAAGTGGAGAGGAAGTCCAAGTCTATCATTGACGAATTCTTACACAT
  CAATGACTTTAAGGAGGCCACGCAGTGCATAGAGGAGCTGAGCGCCCAGGGCCCACTGCATGTGTTCGTG
  AAGGTGGGTGTGGAGTTCACCCTGGAACGGAGCCAGATCACCAGGGACCACATGGGCCACTTACTGTATC
  AGCTGGTGCAGTCAGAAAAACTCAGCAAGCAGGACTTTTTCAAAGGTTTTTCTGAAACCTTGGAGTTGGC
  AGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTGGCTGAACTGGTCACCCCCATGTTAAAA
  GGAGGGGGGATTTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATTACTTCCTGTTGGCAGAGCTG
  GGGTCCTGCTTTCTGAAATCCTACACCTTCTATGCAGACAAATGAGCCATAAGAAAGTAGGCGCCCTGTG
  GAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTACCGGAAGGGGAAGATGTCCATCATTTTCTCTTGGAG
  CAGAAGTTGGACTTCACGGAATCCGAAGGGCCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTG
  CCGAGGAGCTGTCTCAGCGCCTGGAAAAGCTCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGA
  CTGGGTGGAGACTTGCTCCGGATGTTTTTTGATTGCCTGTATGACGAGGAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256198
• Insert Size: 4395 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256198.1, NP_001243127.1
• RefSeq Size: 6069 bp
• RefSeq ORF: 4395 bp
• Locus ID: 230861
• Cytogenetics: 4 D3
• Gene Summary: Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR and lacks an alternate, in-frame, exon in the 3' coding region which causes a shift in the reading frame, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus when it is compared to isoform 1.