Tex14 (NM_001199293) Mouse Untagged Clone

CAT#: MC229597

Tex14 (untagged) - Mouse testis expressed gene 14 (Tex14), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tex14
• Synonyms: C85585
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229597 representing NM_001199293
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCCTGTCTACAAACAAACAAACAAAACAACATGTCTCGGGGGGCTCCTTTTCCAGTCCCTTGCC
  CGGTGCTGCTGGGGACCTTCACAGATGACTCCCTGGAGGCTCAGCTTCACGAATACGCCAAGCAAGGGAA
  CTGCGTGAAGCTCAAGAAGATTCTTAAGAAGGGAGTCTGTGTCGATGCAGTTAACACTCAGGGCCAGTCA
  GCGCTCTTTGTTGCAGCATTACTGGGCCATGTGAAATTAGTGGATGTTCTGGTGGATTATGGATCAGATC
  CAAATCACCGCTGCTTTGACGGGAGCACTCCTGTCCATGCAGCAGCCTTTTCAGGCAACCAGTGGATCCT
  CAGTAAACTGCTGACTGCAGGGGGTGACCTGCGACTCCATGATGAGAAGGGTCGGAATCCACAGGCCTGG
  GCCTTGACAGCAGGGAAGGATCGTAGCACTCAGATGGTGGAGTTCATGCAACGTTGTACTTCACATATGA
  AGGCTATCATCCAGGGCTTCTCCTATGACCTTCTGAAGAAGATAGACTCTCCTCAGCGACTCATCGGCAG
  CCCACCCTGGTTTGGTAGCCTCATTCAGGGAAGCCCAAATAGTTCTCCCAACCGACAGCTTAAACCTGGA
  ATCATTTCTGCCCAAAATATCTACAGCTTTGGCTTTGGGAAGTTTTATCTCACCTCAGGGATGCAGCTTA
  CTTACCCAGGATCTCTTCCAGTCATTGGAGAGAAGGAAGTGGTCCAGGCTGATGATGAGCCCACCTTCTC
  TTTCTTCAGTGGCCCCTACATGGTCATGACTAACCTCGTGTGGAATAGGAGCAGAGTCACAGTAAAGGAG
  CTGAACCTTCCCACCCGTCCCCACTGTAGCAGGCTGAGGTTGGCCGACTTGCTGATTGCTGAGCAGGAGC
  ACAGCAGCAACCTGCGGCATCCTAACCTGCTGCAACTGATGGCTGTATGTTTGTCCCGGGACCTGGAGAA
  AATTCGCCTGGTTTACGAGCGTATCACAGTCGGCACACTGTTCAGTGTCCTCCATGAACGAAGGTCCCAG
  TTCCCAGTGCTGCACATGGAGGTGATTGTGCACCTGTTGCTCCAGGTTGCTGATGCCTTGATATACCTGC
  ATTCCCGGGGGTTCATCCACCGCTCCCTCAGCTCCTACGCTGTCCACATCGTCTCTGCAGGAGAAGCAAG
  GCTGACTAACCTGGAATACCTGACGGAAAGCCAGGACAGTGGTGCACACAGGAACGTGACTCGAATGCCC
  CTCCCCACCCAGCTGTACAACTGGGCTGCACCAGAAGTGGTCTTGCAGAAGGCAGCCACGGTGAAGTCAG
  ACATATACAGCTTTTCCGTGATCATACAAGAGATCTTAACAGACAGTATACCCTGGAATGGCTTGGATGG
  CTCACTTGTTAAAGAAACCATAGCCTTGGGAAATTATTTAGAAGCTGATGTCAGGCTTCCGGAACCTTAC
  TATGATATTGTTAAGTCAGGAATCCATGCCAAGCAGAAGAACCGAACAATGAACCTTCAAGATATTCGTT
  ATATTCTGAAGAATGACTTAAAGGAATTTATTGGAGCTCAGAAAACTCAGCCAACCGAGAGCCCCAGAGG
  GCAGAGCTATGAACCCCATCCTGATGTTAATATCTGCCTAGGTCTAACTTCAGAATATCAAAAGGACCCT
  CCAGACTTGGACATCAAGGAACTAAAGGAAATGGGTAGTCAGCCCCATTCACCTACAGATCACTCCTTTC
  TCACTGTAAAACCAACACTAGCTCCTCAGACCCTAGATTCAAGTCTGTCAGCCCAGAAACCTGACAATGC
  AAATGTTCCTTCTCCTCCTGCTGCATGTCTGGCAGAAGAGGTCAGGAGCCCCACTGCAAGTCAGGACAGC
  CTCTGCAGCTTTGAAATCAATGAGATCTACTCAGGCTGCTTGACACTGGGAACTGACAAGGAGGAAGAGT
  GTCTGGGGACTGCTGCTTCACCTGAGGGGGATAGACCAAACCAGGGAGATGAGCTGCCATCCCTGGAAGA
  AGAGCTCGATAAGATGGAGAGAGAATTGCACTGTTTTTGTGAAGAGGACAAAAGCATTTCAGAAGTTGAC
  ACAGACCTTCTTTTTGAGGATGATGACTGGCAAAGTGATTCTCTTGGTTCACTCAACCTGCCCGAACCAA
  CCAGAGAAGCCAAGGGCAAAACGAGCAGCTGGTCCAAGACTGATGAGTATGTCAGTAAGTGTGTGCTGAA
  TCTGAAGATTTCACAGGTGATGATGCAGCAGAGCGCTGAGTGGCTGAGGAAGCTTGAGCAGGAGGTAGAG
  GAGCTCGAGTGGGCACAGAAGGAGCTGGACAGTCAGTGCAGCAGTTTGCGGGATGCTTCATTAAAGTTTG
  CAAATGCCAAGTTCCAGCCGGCTGTAGGCCCTCCATCTTTGGCCTATCTTCCTCCTGTTATGCAATTACC
  AGGGCTCAAGCAGCCTGAAAATGGTGGCACCTGGTTAACCCTAGCAAGGTCTCCAGGAAATGAGAGAGAG
  TTCCAAGAGGGACATTTTAGCAAAAAACCTGAGAAACTAAGTGCCTGTGGCTGGAAGCCTTTTACACAAG
  TGTCTGAAGAAAGCAGAGGGGACTGCTCAGAGCTAAACAATCAGCTGCCGACTCTTCGTGGTCCTGGGAA
  GCAGAGCACAGGTGAGCAGTTACCATCCACTCAAGAAGCAAGGGAGAGTTTGGAAAAAAATACAAACCAA
  AATAGTAGGAGTATGGCGTCTGTGTCTTCTGAAATCTATGCTACTAAGTCAAGAAATAATGAGGATAATG
  GAGAGGCACACTTGAAATGGAGATTGGCAGTAAAAGAAATGGCAGAGAAAGCAGTTTCCGGACAGCTCTT
  ATTACCTCCTTGGAATCCTCAGAGTAGTGCGCCTTTTGAGAGTAAGGTTGAAAATGAGAGCACTCCTTTG
  CCACGGCCCCCAATTAGAGGTCCTGAGAGCACAGAATGGCAGCACATTTTAGAATACCAGAGGGAAAATG
  ATGAGCCCAAAGGAAATACGAAGTTTGGCAAAATGGACAACAGTGACTGTGACAAGAACAAGCACAGCAG
  ATGGACAGGCCTCCAGCGCTTCACTGGTATTAGATACCCATTCTTCAGAAACCACGAGCAGCCAGAGCAG
  AATGAAGCCTCTCAAGCAAGCTGTGACACGTCTGTGGGCACTGAGAAGTTCTACAGCACCTCAAGTCCCA
  TAGGAGACGACTTTGAAAGATTCCAAGATTCTTTTGCCCAACGTCAAGGCTATGTTGAAGAAAATTTCCA
  AATAAGAGAAATATTTGAAAAGAATGCTGAGATTTTGACCAAGCCTCAGTTTCAAGCTATTCAATGTGCT
  GAAGACAAACAAGACGAAACATTAGGGGAGACGCCAAAGGAACTGAAAGAGAAAAACACATCACTGACAG
  ACATTCAAGACTTGTCCAGCATCACCTATGATCAAGACGGCTATTTTAAGGAAACCTCATACAAAACACC
  CAAATTAAAACACGCACCAACTAGTGCCAGTACCCCGCTAAGCCCAGAGTCGATTTCTTCAGCTGCTAGT
  CACTATGAAGACTGCCTTGAAAATACCACATTTCATGTTAAAAGAGGATCTACATTTTGTTGGAATGGCC
  AAGAAGCTATGAGAACTTTGTCTGCCAAATTTACAACTGTCCGAGAGAGAGCTAAGAGCCTGGAATCACT
  TCTCGCTTCTTCTAAAAGCCTACCTGCCAAGCTGACTGACTCCAAGAGATTGTGTATGTTGAGTGAGACT
  GGCTCTTCTAACGTTTCTGCGGCATTTGTAACATCAACTCATGCTACCAAGAGGAAGAGCCTACCCAGAG
  AACTGGCAGAAGCCACCTCTCAACAGCATCTTGATGAGCTTCCACCACCAGCTCAGGAGCTACTTGATGA
  AATTGAGCAACTGAAGCAGCAGCAGGTCTCATCCCTGGCGTCACATGAGAACACGGCACGTGATCTGAGT
  GTCACTAACAAGGATAAGAAGCATTTGGAAGAACAAGAAACCAACAGTAGTAAAGACAGCAGTTTTCTTT
  CCAGCAGAGAAATTCAGGATCTGGAAGATACAGAGAGAGCTCATTCTTCTCTTGATGAGGACCTGGAAAG
  ATTCCTGCAGTCACCTGAGGAGAACACGGCACTGCTGGACCCTACCAAGGGCTCTACAAGGGAGAAAAAA
  AACAAAGATCAAGACGTTGTTGAGCAGAAGAGAAAAAAGAAAGAAAGCATCAAGCCAGAGAGAAGGGAGT
  CAGACAGCTCCCTAGGGACCTTGGAAGAAGATGAACTAAAACCCTGTTTTTGGAAGCGACTGGGTTGGTC
  CGAACCTTCCAGGATAATTGTGCTGGATCAGAGCGACTTGTCAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199293
• Insert Size: 4389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199293.1, NP_001186222.1
• RefSeq Size: 4795 bp
• RefSeq ORF: 4389 bp
• Locus ID: 83560
• UniProt ID: Q7M6U3
• Cytogenetics: 11 C
• Gene Summary: Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.