Arap3 (NM_001205336) Mouse Untagged Clone

CAT#: MC229596

Arap3 (untagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arap3
• Synonyms: Centd; Centd3; cnt-d3; DRA; Drag1; E030006K04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229596 representing NM_001205336
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAACTCCCAGCCCAGCCCCTGATGCCCAACCCCCCAAGCCTGTGCCTAAGCCCAGGACGGTGT
  TTGGGCTCAGCAACCCTGCCACTGCCCAGAGGCCTGGACTGAGCCCAATCTTCTGGGATCCAGAAGTATC
  CAGGAACTCAGAGTGTACGCAAAGATCCTCTCCTCTCCTTCCTTCCTCCTCTGAACAGCCTTCAGTCCCG
  AATACCATGGAGATGATGCCTAATGCCATCTACTTTGGCCTGGACTTAAGAGGTCGGGCCCAGGCAGCTC
  AGGACGTGACTCCAGATAGTTCTCAGGCGACTGTCCCAACCCCTGCCTTCAGGCCCACAACAGGCACAGT
  GCACATCATGGATCCTGGTTGCCTGTACTACGGTGTCCAGCCTGTGGGGATCCCAGGAGCCTCTGACAGG
  AGAGACGGCAGGGGTGTCTGTCAGGAAAGGGCAGAACACAGACAGGATCTGGAGACACGGGAGGATGCTG
  GTTATGCCAGCCTCGAGCTACCTGGAGACTCCATTCTTTCGCTACCCACCCAGGATGCAGAGACCAGTGA
  TGACCTCATTTCGCCCTATGCCAGCTTCTCCTCCACAGCAGATCGTCCCGTGCCTCTGCTCAGTGGCTGG
  CTGGACAAGCTCTCACCTCAGGGAAACTATGTGTTCCAGAGACGTTTTGTGCAGTTCAATGGAAGGAGTC
  TAATGTACTTTGGCAGTGACAAGGACCCCTTCCCCAAAGGTGTGATCCCTCTGACTGCCATCGAGATGAC
  CCGAAGCAGCAAGGACAACAAATTCCAGGTCATTACCGGCCAGAGAGTATTTGTGTTCCGCACAGAGAGT
  GAGGCTCAGCGTGACCTATGGTGCTCCACGCTGCAATCCTGCCTGAAGGAACAGCGCCTCCTGGGCCACC
  CCCGGCCCCCTCACCCACCACGACCCCTCCGCACGGGCACGTTAGAGTTGCGTGGACACAAGGCCAAGGT
  GTTTGCTGCCTTGATCCCTGGAGAGCTGGCACTGTACAAGAGTGAGCAGGCCTTCTCACTGGGCATCGGG
  ATCTGCTTCATTGAACTGCAAGGCTGCAGTGTCCGGGAGACCAAGAGTCGAAGCTTCGATCTGCTCACAC
  CCCATCGCTGCTTCAGCTTCACAGCCGAGTCTGGGGGGGCTCGACAGAGCTGGGCAGCCGCTCTGCAAGA
  AGCAGTAACCGAGACACTGTCTGACTACGAGGTGGCTGAGAAGGTCTGGTCCAACCCGGCAAACCGGCAT
  TGTGCGGACTGCAGGGCCTCCCGGCCGGATTGGGCTGCCGTCAACCTGGGGGTCGTCATCTGCAAGCAGT
  GTGCAGGTCAGCACAGGGCCCTGGGCTCTGGGATCTCCAAGGTGCAGAGCCTGAAGCTGGACACGAGTGT
  CTGGAGTAATGAGATAGTGCAGCTGTTCATTGTCCTGGGAAATGATCGTGCCAATTGCTTCTGGGCAGGG
  GCACTTCCCCCAGGTGAAGGGCTGCATCCAGATTCAGCCCCTGGCCCTCGAGGAGAGTTCATCTCCCGAA
  AGTATAAGCTGGGTCTCTTCCGGAAGCCCCACCCTCGGCATCCAGATCATAGCCAGCTTCTCCAGGCACT
  GTGTGCAGCTATGGCAGGACCCAACCTGCTCAAGAACATGGCCCAGCTTCTCTGTGTCGAAACCTCTGAG
  GGAGAGGAACCCTTGTCCCCTTCAGCCCTCAATGGCAGCTTGCTTAGCCTTCTACCTTCAGACTCCCCTG
  GAGTATACAATGAGGTGGTAGTGCCTGCCACTTACAGAGGTTTCCTGTACTGTGGCTCCATCAGCAACAA
  GGCTGGAGCCCCACCCCTTCGCAGGGGCCGGGATGCTCCTCCCCGCTTGTGGTGTGTGCTGGGAGCTGCC
  CTGGAAATGTTTGCATCAGAGAGCAGCCCTGAACCCCTCAGCCTTCTCCAGCCCCAGGATATTGTATGTC
  TGGGTGTAAGCCCCCCACCTGCTGATCCAGGTGACCTTGACAGGTTCCCCTTTTCCTTTGAACTCATCCT
  CACTGGGGGAAGAATCCAGCATTTTGCCACTGATGGAGCAGACAGTCTAGAGGCCTGGATCAGTGCAGTG
  GGCAAGTGGTTCTCCCCGCTCAGCTGCCACCAGCTGCTGGGCCCTGGGTTGCTGCGGATGGGTCGCCTGT
  GGCTACGGTCCCCCTCCCACGCAGGCCTGGCCCCTGGTCTCTGGCTGTCGGGCTTCGGCCTCCTTCGTGG
  TGATCATCTCTTCCTGTGCCCAGCGCCTGGCCCTGGTCCCCCTGCTCCGGAGGACATGGTGCATTTACGA
  CGTCTACAGGAGATCAGTGTGGTCTCGGCAGCTGACACTCCAGACAAAAAGGAGCATTTGGTCCTTGTAG
  AGACAGGAAGGACCCTGTATTTGCAGGGGGAAGGCAGGCTAGACTTTGCAGCGTGGAACACGGCCATTGG
  AGGTGCTGCCGGAGGGGGTGGCACGGGCCTGCAGGAGCAGCAGATGAGCCGGGGAGACATCCCTATCATT
  GTGGACGCTTGCATCAGTTTTGTCACGCAGCATGGGCTCCGGCTGGAAGGTGTATATCGGAAAGGAGGCG
  CTCGAGCCCGCAGCCTGCGACTCTTGGCTGAGTTTAGGCGGGATGCCCGGTCAGTGAAGCTTAGACCACG
  GGAACACTTTGTAGAAGATGTTACTGACACACTCAAACGCTTCTTTCGAGAGCTTGATGACCCCGTGACC
  TCTGCTAGGTTGCTGCCTCGATGGAGAGAAGCTGCAGAGCTTTCCCAGAAGAATCAGCGCCTGGAGAAGT
  ACAAAGAAGTGATTAGCTGCCTGCCTCGAGTCAACCGCAGAACACTGGCCACCCTCATCGGGCATCTGTA
  CCGGGTGCAGAAGTGTGCGTCTCTAAACCAGATGTGCACGCGGAACCTGGCCCTACTGTTTGCACCTAGT
  GTGTTCCAGACAGATGGGCGGGGAGAGCATGAGGTTCGGGTACTGCAGGAGCTCATCGATGGCTACATCT
  CTGTTTTTGATATTGACTCGGACCAGGCAGCTCAGATTGACTTGGAGGTCAGTCTCATTACCACCTGGAA
  GGATGTGCAGTTGTCCCAGGCTGGAGACCTCATCATGGAGGTTTACATAGAACAGCAGCTCCCGGACAAC
  TGTGTCACCCTAAAGGTATCCCCAACACTGACTGCTGAGGAGTTGACTAACCAGGTGCTGGAGATGCGAG
  GGGCGGCATCGGGGACAGACTTGTGGGTGACATTTGAAATTCTTGAACACGGAGAGCTTGAGAGGCCACT
  GCACCCCAAAGAAAAGGTTCTAGAGCAGGCCTTGCAGTGGTGCCAGCTTCCAGAGCCCTGCTCAGCCTCT
  CTGCTTCTGAGAAAAGTCTCTATGGCACACGCTGGCTGCCTCTTCACAGGAGTCCGGCGTGAGAGCCCAC
  GAGTGGGGCTGCTACGGTGTCGTGAGGAGCCACCTCGATTGCTGGGAAACCGTTTCCAGGAGAGGTTCTT
  TTTGGTGCGTGGCCGATGCCTGCTGCTGCTCAAGGAGAAGAAGAGCTCTAAGCCAGAACGGGAGTGGTCT
  CTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCAAGAAGTTAAAACCTCCAACACTGTGGGGTTTCACAT
  TAATATTGGAAAAGATGCACCTCTGTCTGTCCTGCATGGATGAGGAGGAGATGTGGGACTGGACCACAAG
  CATCCTTAAAGCCCAGCATGATGACCAACAGTCCGTGGTTTTACGTCGCCGTTCCTCCTCTGACCTTGCT
  CGTCAGAAGTTTGGCACTATGCCCTTGCTGCCCATCCGAGGGGATGACAGTGGAGCCACCCTTCTCTCTG
  CCAATCAGACCCTGCGGCGACTACACAACCGGAGGACCTTATCCATGTTCTTTCCAATGAAGTCACCCCA
  GGGATCTGTGGAAGAGCAAGATGAGCTGGAGGAGCCTGTGTATGAAGAGCCAGTATATGAGGAGGTCGGG
  GCCTTCCCTGAGCTTACCAAGGACACGACCTTCTCTTCCACATGGGAGTGGTCAGCCAAGTCAGATCCAT
  CCCTTACCAGCCAGAGGTCCTTTGATCAACCCCCCCTCTCCAAGGCAAGCATGTTGGGCCACGAAGAGAG
  GATACCTGACCCTCCTCCGGGCCCCCCTTCAAAAAGCAGTTCTCAGGCACGAGGGTCCCTAGAGGAACAG
  CTGCTTCAGGAACTCAACAACCTCATCCTGAGGAAAGGAGAGCCTGCCTCGTGCCCAGAAAGCTCTTCCC
  AGCCCACCAGTCCCCAAGCCCCAAGCCCCACCAGCCTTCCAACACCGACCCCCAGCTTACCCACCCAACC
  GCCCTGCACTTCCAATCCACCCTCCAGCCAGCCTCTCACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205336
• Insert Size: 4383 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205336.1, NP_001192265.1
• RefSeq Size: 5425 bp
• RefSeq ORF: 4383 bp
• Locus ID: 106952
• UniProt ID: Q8R5G7
• Cytogenetics: 18 B3
• Gene Summary: This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.