Ptprt (NM_001291151) Mouse Untagged Clone

CAT#: MC229592

Ptprt (untagged) - Mouse protein tyrosine phosphatase, receptor type, T (Ptprt), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprt
• Synonyms: mKIAA0283; mRPTPrho; R-PTP-T; RPTP-rfo; RPTP-rho; RPTPmam4; RPTPrho
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229592 representing NM_001291151
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCCTTGGCGGGCTCGCCCTCTGCCTGCTCCGGCTCCTGCTCCTGGGGCTGCAGCGGCCGCCGT
  TGCCCGGCGCCGGAGCGCAGAGCGCCGCAGGTGGCTGTTCTTTTGACGAACATTACAGCAACTGCGGGTA
  TAGCGTGGCTCTGGGAACCAATGGGTTTACCTGGGAGCAGATTAACACATGGGAGAAGCCAATGCTGGAC
  CCAGCTGTGCCCACAGGGTCCTTCATGATGGTGAACAGCTCTGGAAGGGCTTCAGGCCAGAAAGCCCATC
  TTCTTCTGCCAACCTTGAAGGAGAATGACACTCACTGCATTGACTTCCATTACTACTTCTCCAGTCGAGA
  TCGCTCCAGCCCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGACCCCAAGGGAACCCTGTCTGG
  AACGTATCTGGCGTCGTCACTGAGGGCTGGGTGAAGGCAGAGCTTGCCATCAGCACCTTCTGGCCTCATT
  TCTATCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTACATCGCTGTGGACGAAGTTCG
  GGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTGCGACTCCAAAACGTTGAGGTGAATGTGGGG
  CAGAACGCCACGTTTCAGTGCATTGCTGGTGGAAAGTGGTCCCAGCATGACAAACTTTGGCTCCAGCAAT
  GGAATGGCAGAGACACAGCCCTCATGGTCACCCGGGTGGTCAACCACAGACGCTTCTCAGCCACAGTGAG
  TGTGGCAGACACCTCTCAACGCAGTATCTCCAAGTATCGCTGCGTGATCCGCTCAGATGGTGGGTCTGGT
  GTGTCCAACTATGCAGAGCTGATTGTGAAAGAGCCTCCCACGCCCATTGCTCCCCCGGAACTACTGGCCG
  TGGGTGCCACCTACCTGTGGATTAAACCAAATGCCAACTCCATTATTGGGGATGGCCCCATCATACTGAA
  GGAGGTAGAATACCGCACAACCACAGGAACCTGGGCCGAGACCCACATCGTGGACTCTCCCAACTACAAG
  CTCTGGCATCTGGACCCTGATGTGGAGTATGAGATCCGGGTGCTGCTCACACGACCAGGAGAGGGGGGCA
  CAGGACCACCAGGACCACCCCTAACTACCAGGACCAAGTGTGCCGATCCCGTGCATGGCCCGCAGAATGT
  GGAGATTGTGGACATTCGGGCTCGGCAGCTGACCCTGCAGTGGGAACCCTTTGGCTATGCAGTGACCCGC
  TGCCACAGCTACAACCTCACAGTGCAGTACCAGTACGTGTTCAACCAGCAGCAGTATGAAGCTGAAGAGG
  TGATCCAGACATCTTCCCACTATACCCTTCGGGGTCTACGGCCCTTCATGACCATCAGACTGCGGCTGCT
  ACTGTCCAACCCTGAGGGCCGGATGGAGAGTGAGGAGTTGGTGGTACAGACAGAAGAGGATGTTCCAGGA
  GCTGTTCCTCTCGAGTCCATCCAAGGGGGTCCCTTTGAGGAGAAGATCTACATCCAGTGGAAACCTCCCA
  ATGAGACCAATGGGGTCATCACACTCTATGAGATTAACTACAAGGCTGTGGGCTCACTGGATCCAAGTGC
  TGACCTCTCCAGCCAGAGGGGAAAGGTGTTCAAACTCCGGAATGAAACCCACCACCTCTTTGTGGGTCTG
  TACCCTGGGACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCCCCAGTCACTA
  CTCGGATTGCCACCAAGATTTCAGCTCCATCAATGCCTGAATATGACGCAGATACCCCACTCAATGAAAC
  AGATACAACCATCACGGTGATGCTCAAACCCGCCCAGTCCAGGGGAGCCCCAGTCAGTGTTTACCAGCTG
  GTTGTCAAGGAGGAGCGACTCCAGAAGTCTCGGAGAGCAGCTGACATCATTGAGTGTTTCTCAGTACCTG
  TGAGCTACCGGAACGCCTCCAACCTCGATTCGCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAACCT
  GCCTGTCACCCAGCCATTTACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAACCCTCCTCTCTCC
  CCATTGAAGAGCTACAGCATCTACTTCCAGGCCCTCAGCAAAGCAAATGGAGAGACAAAAATCAACTGTG
  TGCGTCTGGCTACAAAAGGTGCTTCCACTCAGAATTCTAACACTGTGGAGCCGGAGAAGCAGGTCGACAA
  CACGGTGAAGATGGCTGGCGTGATTGCTGGCCTCCTCATGTTCATCATCATTCTCCTGGGGGTGATGCTG
  ACCATCAAAAGGAGAAGAAATGCTTATTCGTACTCCTATTACTTGAAGCTGGCTAAGAAGCAGAAGGAGA
  CCCAGAGTGGAGCCCAGAGGGAGATGGGTCCTGTGGCCTCGACTGACAAGCCTACCGCCAAGCTCGGCAC
  CAACCGCAATGATGAAGGCTTCTCCTCCAGCTCTCAGGATGTTAATGGATTCAATGGCAGCCGTGGGGAG
  CTGTCTCAGCCTACCCTCACCATTCAGACTCATCCCTACCGGACTTGTGACCCTGTAGAGATGAGCTATC
  CCCGGGACCAGTTCCAGCCTGCCATCCGGGTGGCGGACCTGCTTCAACACATCACCCAGATGAAGAGAGG
  CCAGGGCTACGGGTTCAAGGAGGAATACGAGGCCTTACCAGAAGGACAGACAGCTTCGTGGGACACAGCC
  AAGGAAGATGAAAACCGCAATAAGAATCGATACGGAAACATCATATCTTATGACCACTCTCGAGTAAGGC
  TGTTGGTGCTGGATGGAGACCCTCACTCAGACTACATCAATGCCAACTACATTGACGGGTACCACCGACC
  CCGGCACTACATTGCAACCCAAGGTCCAATGCAAGAGACGGTGAAGGACTTTTGGAGAATGATCTGGCAG
  GAAAACTCTGCCAGCATCGTCATGGTCACAAACCTTGTGGAAGTGGGCAGGGTGAAGTGTGTCCGATACT
  GGCCAGATGACACAGAGGTCTATGGAGACATTAAAGTCACCCTAATAGAAACAGAGCCCCTGGCAGAATA
  CGTCATCCGCACCTTCACAGTCCAGAAGAAAGGCTACCATGAGATCCGGGAGCTCCGCCTCTTCCACTTC
  ACCAGCTGGCCTGACCACGGTGTTCCCTGCTATGCCACTGGCCTTCTGGGCTTTGTCCGCCAGGTCAAGT
  TTCTCAATCCCCCAGAAGCTGGGCCCATAGTGGTCCATTGCAGTGCTGGAGCCGGGAGGACTGGCTGCTT
  CATTGCGATTGACACCATGCTCGACATGGCTGAGAATGAAGGGGTTGTGGACATCTTCAACTGTGTGCGT
  GAGCTCCGGGCACAGAGGGTCAACCTGGTGCAGACAGAGGAGCAGTACGTGTTTGTGCACGATGCCATCC
  TGGAAGCATGCCTCTGCGGCAATACTGCCATCCCAGTGTGTGAGTTCCGCTCTCTCTACTACAACATCAG
  CAGGCTGGACCCGCAGACCAACTCCAGCCAGATCAAAGACGAGTTTCAGACACTCAACATTGTGACACCT
  CGAGTGCGGCCTGAAGACTGCAGCATTGGGCTCTTACCCCGGAACCATGATAAGAATCGGAGCATGGATG
  TCCTGCCTCTGGACCGCTGTCTACCCTTCCTCATCTCAGTAGATGGAGAGTCCAGCAACTACATCAATGC
  AGCACTGATGGATAGCCACAAGCAGCCTGCCGCCTTCGTGGTCACCCAGCATCCTCTACCCAACACGGTG
  GCAGACTTCTGGAGGCTGGTGTTTGATTATAATTGTTCATCTGTGGTGATGCTGAACGAGATGGATACTG
  CTCAGCTCTGTATGCAGTACTGGCCTGAGAAGACCTCCGGGTGTTATGGTCCCATCCAGGTGGAGTTTGT
  CTCTGCAGACATCGATGAGGACATCATCCACAGAATCTTCCGGATCTGTAACATGGCTCGGCCACAGGAT
  GGTTATCGTATTGTCCAGCACCTCCAGTACATCGGCTGGCCTGCATACCGGGACACGCCCCCCTCTAAGC
  GCTCTCTGCTCAAAGTGGTCCGACGGCTGGAGAAATGGCAGGAGCAATACGACGGAAGAGAGGGGCGCAC
  TGTGGTCCACTGCCTAAATGGGGGAGGCCGCAGTGGAACCTTCTGTGCTATCTGCAGTGTGTGTGAGATG
  ATCCAGCAGCAGAACATTATTGACGTGTTCCACATTGTGAAAACCCTCCGCAACAACAAGTCCAACATGG
  TGGAGACGCTGGAACAGTATAAATTTGTATATGAGGTGGCACTGGAATATTTAAGCTCCTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291151
• Insert Size: 4335 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291151.1, NP_001278080.1
• RefSeq Size: 12126 bp
• RefSeq ORF: 4335 bp
• Locus ID: 19281
• Cytogenetics: 2 81.91 cM
• Gene Summary: May be involved in both signal transduction and cellular adhesion in the CNS. May have specific signaling roles in the tyrosine phosphorylation/dephosphorylation pathway in the anterior compartment of the adult cerebellar cortex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site and contains an additional in-frame exon but lacks a different in-frame exon in the central coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.