Dapk1 (NM_001285917) Mouse Untagged Clone

CAT#: MC229591

Dapk1 (untagged) - Mouse death associated protein kinase 1 (Dapk1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dapk1
• Synonyms: D13Ucla1; DAP-Kinase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229591 representing NM_001285917
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTGTTCAGGCAGGAAAACGTGGACGACTACTACGACACCGGCGAGGAACTGGGCAGTGGACAGT
  TCGCAGTTGTGAAGAAATGTCGTGAGAAAAGTACCGGTCTTCAGTATGCGGCCAAGTTCATCAAGAAAAG
  GAGGACCAAGTCCAGCCGGCGGGGCGTGAGCCGGGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG
  ATCCGGCACCCAAATGTCATCACCCTGCATGAGGTCTATGAGAACAAGACAGATGTCATTCTGATCCTGG
  AGCTTGTTGCAGGAGGTGAGCTGTTTGACTTCCTGGCTGAGAAGGAATCTCTGACTGAAGAGGAGGCAAC
  GGAATTCCTTAAGCAGATTCTCAGCGGCGTTTACTACCTGCACTCACTGCAGATCGCTCACTTTGACCTG
  AAGCCGGAAAACATAATGCTTCTGGATAGAAATGTGCCCAAACCTCGGATCAAGATCATAGACTTTGGCT
  TGGCCCATAAAATTGACTTTGGAAATGAATTCAAAAACATATTTGGGACACCAGAGTTTGTGGCTCCGGA
  GATAGTCAACTATGAGCCCCTGGGTCTTGAGGCAGATATGTGGAGCATCGGGGTAATAACCTATATCCTC
  CTAAGTGGGGCCTCCCCTTTTCTTGGAGACACCAAGCAAGAAACATTAGCGAATGTGTCCGCTGTCAACT
  ACGACTTTGAGGAGGAATTCTTCCGGAACACCAGTACCCTTGCCAAAGATTTCATCAGAAGACTGCTGGT
  CAAGGATCCAAAGAAGAGGATGACAATCCAGGACAGTTTGCAGCACCCCTGGATCAAGCCTAAAGACACC
  CAACAAGCACTTAGTCGAAAAGCCTCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCTCGGAAAA
  AATGGAAACAATCTGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTTTTGTCCAGAAGTAA
  CATGAGTGTCGCCAGGAGTGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT
  GCCATCAATGATGACAACGTACCCGGCCTGCAGCATCTCCTGGGCTCCTTGTCCAGCTATGACGTCAACC
  AGCCCAACAAGCATGGGACACCTCCATTACTGATTGCCGCAGGCTGTGGCAACATCCAGATGTTACAGTT
  ACTCATAAAACGAGGCTCAAGGATTGACGTCCAGGATAAGGGAGGATCCAATGCCATCTACTGGGCCTCT
  CGGCATGGCCATGTGGATACTTTGAAATTTCTCAATGAGAACAAATGCCCTTTGGATGTTAAAGACAAGT
  CTGGAGAGACAGCTCTTCACGTGGCAGCCCGCTATGGCCATGCAGATGTGGTTCAACTACTGTGCAGTTT
  TGGCTCTAATCCTGATTTCCAGGACAAGGAAGAGGAAACCCCCCTGCACTGTGCTGCCTGGCATGGCTAT
  TACTCCGTGGCTAAAGCTCTTTGTGAAGTTGGCTGCAACGTGAATATCAAGAATCGGGAGGGAGAGACCC
  CATTGCTGACGGCGTCTGCCAGGGGCTATCATGACATTGTGGAGTGTCTGGCTGAACATGGAGCTGACTT
  GAATGCTTCTGACAAGGATGGACACATCGCTCTTCATCTTGCTGTGAGGCGTTGTCAGATGGAAGTCATC
  AAGACCCTCCTTGGCCATGGGTCCTTTGTGGATTTCCAGGACAGGCATGGCAACACACCCCTGCACGTGG
  CCTGCAAAGATGGAAGCGCACCTATCGTGGTGGCCCTCTGTGAAGCCAGCTGCAATCTGGACATCTCAAA
  CAAGTATGGTCGGACTCCTCTCCACCTTGCAGCCAACAACGGGATCCTAGATGTGGTCCGCTACCTCTGT
  TTGATGGGCGCCAATGTGGAGGCTCTAACCTCGGATGGAAAGACGGCCGAGGACCTCGCCAAGGCAGAAC
  AGCACGAGCATGTGGCAGGGCTCCTGGCAAGACTGCGGAAGGACACACACCGAGGACTCTTCATCCAGCA
  ACTCCGACCCACCCAGAATCTCCAGCCCAGAATCAAGCTCAAACTGTTTGGCCATTCGGGATCAGGGAAA
  TCCACCCTGGTGGAATCTCTCAAGTGTGGGCTGTTAAGGAGTTTCTTCAGAAGGCGCCGGCCCAGACTAT
  CCTCTACCAACTCCACCCGCTTCCCACCGTCACCCCTGGCTGCTAAGCCAACAGTCTCAGTGAGCATTAA
  CAACCTGTACCCCGGCTGTGAGAACGTGAGCGTAAGGAGCCGCAGCATGATGTTCGAGCCGGGCCTCACC
  AAAGGGATGCTGGAAGTGTTCGTGGCTCCGTCTCACCACCTCCACTGCTCGACTGATGACCAGTCCACCA
  AAGCCATCGACATCCAGAATGCTTATTTGAACGGAGTTGGTGATTTCAGTGTGTGGGAGTTCTCTGGAAA
  CCCTGTGTACTTCTGTTGCTATGACTACTTTGCTGCCAACGACCCCACGTCCATCCACATCATCGTTTTC
  AGTCTCGAAGAACCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCTCTGG
  TCCCAGTTGAAGAACCCATAGCATTTGGAGGCAAGCTGAAGAACCCTCTCCGAGTTGTCCTGGTGGCAAC
  ACATGCTGACATCATGAACATCCCTCGGCCTGCTGGAGGCGAGTTTGGATATGACAAGGATACATCCTTG
  CTGAAAGAGATCAGAAACAGGTTCGGGAATGACCTTCATGTCTCAAACAAGCTGTTTGTCCTGGATGCAG
  GAGCGTCTGGGTCTAAGGACATTAAGGTTCTTCGGAATCACCTGCAAGAAATACGGAGCCAGATTGTCTC
  GGGGTGTTCGCCCATGACTCACCTGTGCGAGAAGATCATCTCCACGCTGCCCTCCTGGCGGAAGCTCAAC
  GGGCCAAACCAGCTGATGTCGCTGCAGCAGTTTGTGTATGACGTGCAGGACCAGCTGAACCCCCTGGCCT
  CTGAGGATGACCTCAGGCGCATTGCACAACAGCTACACAGCACGGGCGAGATAAACATCATGCAGAGTGA
  GACAGTGCAGGACGTGTTACTGCTGGATCCTCGATGGCTCTGCACCAACGTCCTGGGGAAGCTGCTGTCT
  GTGGAGACGCCCCGAGCCCTGCACCATTACCGGGGCCGCTACACCATGGAAGACATCCAGCGTCTGGTCC
  CAGACAGTGACGTGGAGGAGCTGCTGCAGATACTGGATGCCATGGACATCTGCGCCCGAGACCTGAGTAG
  TGGGACTATGGTGGATATCCCTGCTCTGATCAAAACAGACAGCCTGCAGCGCTCCTGGGCGGATGAGGAG
  GATGAGGTGATGGTGTACGGTGGGGTGCGCATTGTCCCTGTAGAGCACCTCACCCCCTTCCCCTGTGGCA
  TCTTTCACAAAGTTCAGGTCAACCTGTGCCGATGGATCCACCAGCAAAGCGCGGAGGGTGATGCGGACAT
  CCGTCTGTGGGTGAGCGGCTGCAGGATCGCCAACCGCGGGGCTGAGTTGCTGGTGCTGCTGGTCAATCAC
  GGTCAGGGCATCGAGGTACAGGTGCGTGGCCTGGAGACGGAGAAGATTAAGTGCTGCCTGCTGCTGGACT
  CGGTGTGCAGCACCATCGAGACCGTCATGGCCACCACCTTGCCAGGGTTGTTGACGGTGAAGCACTACCT
  GAGCCCCCAGCAGCTAAGGGAGCACCACGAACCGGTCATGGTCTATCAGCCCCGGGACTTCTTCCGTGCG
  CAGACCCTGAAAGAGAGTTCCCTTACCAACACCATGGGTGGGTACAAGGAGAGCTTCAGCAGCATCACGT
  GCTTTGGGTGTCATGACGTCTACTCACAGGCCAGTCTTGGCATGGATATCCATGCGTCAGACCTGAGTCT
  CCTGACCCGGAGGAAACTGAGTCGCCTGCTTGACCCACCCGACCCCATGGGGAAGGACTGGTGCCTTCTG
  GCCATGAACTTGGGCCTCCCAGACATGGTGGCCAAACACAACGTCAACAACAGGGCTTCTAGGGATTTCC
  TCCCTAGCCCAGTGCATGCCTTGCTACAGGAATGGACCTCCTACCCTGAGAGCACGGTGGGCATCCTTAT
  ATCCAAACTTCGGGAGCTGGGGCGCCGGGATGCTGCGGACTTTTTACTGAAGGCCTCCTCTGTGTTCAAG
  ATCAACCTTGATGGCAATGGCCAGGAGGCCTATGCCTCAAGCTGTAACAGTGGCACGTCCTACAATTCCA
  TTAGCTCAGTGGTGTCCCGGAGAGACTCCCATGCTTGGACCCCACTGTATGATTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285917
• Insert Size: 4329 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285917.1, NP_001272846.1
• RefSeq Size: 5398 bp
• RefSeq ORF: 4329 bp
• Locus ID: 69635
• UniProt ID: Q80YE7
• Cytogenetics: 13 32.53 cM
• Gene Summary: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3 (By similarity). Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 3' UTR and 3' coding region, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1.