Ssh2 (NM_001291190) Mouse Untagged Clone

CAT#: MC229589

Ssh2 (untagged) - Mouse slingshot homolog 2 (Drosophila) (Ssh2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ssh2
• Synonyms: mSSH-2L; SSH-2; SSH-2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229589 representing NM_001291190
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGAGCACTTTGGCAGGCGAAAGGAAAGCGCTCCCTGCCAGCACCTGCAGCCTCGGTGGCCCCG
  ACATGATCCCCTACTTCTCTGCCAATGCGGTCATCTCGCAGAACGCCATCAACCAGCTCATCAGCGAGAG
  CTTCTTAACTGTCAAAGGTGCTGCCCTTTTTCTACCACGAGGGAATGGCTCGTCTACACCAAGAGTCAGC
  CACAGACGCAACAAGCATGCAGGTGATCTCCAACAGCATCTTCAAGCAATGTTCATATTACTCCGCCCAG
  AAGACAACATAAGGCTGGCTGTAAGACTGGAAAGTACTTACCAAAACCGAACACGCTATATGGTCGTGGT
  TTCAACTAATGGTAGACAAGACACTGAAGAAAGCATTGTCCTGGGAATGGATTTTTCTTCTAATGACAGC
  ACTTGTACCATGGGCTTGGTCTTGCCTCTCTGGAGTGACACCCTAATTCATTTGGATGGTGATGGTGGGT
  TCAGCGTATCAACAGATAACAGAGTTCACATATTCAAACCTGTATCTGTGCAAGCAATGTGGTCTGCACT
  ACAGAGTTTGCACAAGGCTTGTGAAGTGGCCAGAATGCATAACTACTATCCAGGCAGCCTTTTTCTCACT
  TGGGTGAGTTATTACGAGAGCCATATCAACTCAGATCAGTCCTCAGTCAATGAGTGGAATGCTATGCAGG
  ATGTGCAGTCTCATCGGCCTGACTCTCCAGCCCTCTTCACAGACATACCAACTGAACGTGAGCGAACAGA
  AAGGCTTATTAAGACTAAATTAAGGGAGATCATGATGCAAAAGGATTTGGAGAATATCACCTCCAAAGAG
  ATACGCACGGAGCTGGAGATGCAGATGGTGTGCAACTTGCGAGAATTTAAGGAATTCATAGATAATGAAA
  TGATCGTGATCCTTGGTCAGATGGACAGTCCCACACAGATATTTGAGCATGTATTCCTGGGCTCAGAATG
  GAATGCCTCGAACTTAGAGGACTTGCAGAACCGAGGGGTACGGTATATCTTGAATGTTACTCGAGAGATA
  GATAACTTCTTCCCTGGAGTCTTTGAGTATCATAACATTCGAGTATATGATGAAGAAGCAACAGATCTCC
  TGGCTTACTGGAATGACACTTACAAATTCATCTCTAAAGCAAAGAAACATGGATCTAAATGTCTTGTGCA
  CTGCAAAATGGGGGTGAGTCGCTCAGCCTCCACTGTGATTGCCTATGCAATGAAGGAGTATGGATGGAAT
  CTGGATCGAGCCTATGACTATGTGAAGGAAAGACGAACGGTGACCAAGCCTAACCCCAGCTTCATGAGAC
  AACTGGAAGAATACCAAGGGATCTTGCTGGCCAGCAAACAGCGGCATAACAAACTCTGGAGATCTCATTC
  AGATAGTGACCTTTCAGACCACCACGAACCCATCTGTAAACCTGGGCTCGAGCTCAACAAGAAGGAGATG
  ACCACGTCAGCAGACCAGATCGCTGAGGTGAAGACTGTGGAGAACCTTGCTGCCATGCCTACCGTCTTTA
  TGGAACATGTGGTCCCACAAGATGCAAATCAGAAAGGACTACACACCAAAGAAAGAGTCATCTGCTTGGA
  GTTTTCTTCACAAGAGTTTCGTGCCGGACAGATTGAAGATGAATTAAATTTAAATGACATCAATGGATGC
  TCATCAGGGTGTTGTCTCAGTGAATCAAAACTCCCTCTTGACAACTGCCATGCGTCTAAAGCCTTACTCC
  AACCTGGACAGGCCCCAGACATTGCCAACAAGTTCCCAGACTTAGCTGTGGAAGATCTGGAGACAGACGC
  ACTGAAAGCAGACATGAATGTCCACCTACTGCCAATGGAAGAGTTGACATCTCGACTGAAAGACCTCCCC
  ATGTCACCTGATCTGGAGTCACCAAGCCCCCAAGCCAGTTGCCAAGCTGCCATCTCAGATTTTAGTACAG
  ATCGTATTGACTTTTTTAGTGCCCTGGAGAAATTTGTAGAGCTTTCTCAAGAAACCCGGTCTCGGTCTTT
  TTCTCACTCAAGGATAGAAGAATTAGGTGGAGGAAGGAGTGAGGGCTGTCGTTTGTCAGTGATAGAAGTG
  GCAGCTTCAGAAATGGCAGCTGATGACCAGAGAAGCAGCTCTTTGAGTAATACGCCCCACGCCTCTGAAG
  AGTCTTCTGTGGATGAGGACCAGTCGAAGGCAATCACCGAACTTGTCAGCCCAGACATCATCATGCAATC
  TCACTCAGAAAATGCAATTTCAGTCAAAGAAATTGTGACTGAAATTGAGTCCATTAGTCAAGGAGTTGGG
  CAGGTTCAGTTAAAAGGAGACATCCTCTCTAACCCATGCCACACACCAAAGAAGAGCACCATTCATGAGC
  TGCCCCTGGAGAGGGTGCCAGCCCCTGAGAGCAAACCTGGACATTGGGAGCAGGATGAGAGTTTCTGTAG
  TGTCCAGCCAGAACTAGCTAGAGACTCAGGGAAGTGTGCCCCGGAGGAAGGCTGTCTGACCACACACTCA
  TCCACAGCAGACTTGGAAGAAGAAGAACCAGTTGAGGGGGAACATGACTGGGGCCCAGGGATGCACTCTG
  GTGCTAAGTGGTGCCCTGGGTCTGTGAGGCGAGCCACCCTGGAGTTTGAAGAGCGCTTGCGACAAGAGCA
  AGAGAACCATGGTACAGCCTCTGCAGGCCCCACATTATCCAATCGCAAAAACTCTAAGAATGATTCTTCT
  GTGGCAGACTTAATGCCAAAATGGAAAAGTGATGAAACTACCCCAGAACATTCATTTTTCCTCAAAGAAG
  CCGAGCCGAGCAAGGGTAAAGGGAAATGCAGTGGTTCTGAAGCCGGATCACTATCCCACTGTGAGCGTAA
  TCCCACCATGCCAGACTGTGAGCTGCTGGAGCATCATTCCTTGCCGGCTCCTCAGGACTGCCTAGGGTCA
  GATAGTAGAAGTAAGAAGCAGGAAGGAGACCTAAAGAAGCAGAGGGCTGTGGTTCCAAACCAGGAATGTG
  ACACACAGGCCATCCTTCTCCCCCTTCCCAAGAAAATAGAAATCATTGAGTATACGCCGACAGTTACATC
  ACTGGGTCACACCGAGCCAGGAGGTGAGGCGACACCCAGCAAAGAGGGTGAGAAACAAGGACTGAGGAAA
  GTGAAGATGGAGCAGTCTATCACTATGTTTTGTGCGCTGGATGAAAATCTGAACAGGACTCTGGAGCCCA
  GTCAAGTTTCTCTGCATCCCCAAGTGCTACCTCTGCCTCATTCTTCCTCAGAGTGTGACAGGCCCGCTGA
  CCCAAACCCTATGTTAAGTAGCCCGCAAGACAAAGGGGACTGCCCATCCACACCCTTCAAGACAGCAGCA
  CCTTTTGTCAGCTGCAGTACCCAGGGAGCATCTTTCAGCTTGGATTATTTGCTTCCCCACTCTGTGGTTC
  ACCTGGAAGGCTGCACAGAGCAAAGCAGTGCCACTGACAATGAACTGTCTCCAGAGCAGGCTAGCTGGGA
  GGACAGCCGGGGGCATTTCCTCTCCAGTGGTAGTGGAATGGCACACACATCCTCCCCATTAACTAACGAA
  GACCTAAGTTTAATTAACAAACTTGGTGACAGTGTTGGTGTGTTACAGAAAAAACTGGACCCGTCACCTG
  AAGCCTGTAGAATCCCACATAGCTCTAGTAGTGAAAATATAAGAGATCTCAGCCACAGCCGTGGTGTGGT
  GAAGGAGCATGCTAAAGAAATTGAGTCTCGAGTGATCTTCCAGGCAGGGTTTTCCAAAACATCCCAAATG
  AAGCGCTCAGCTTCCCTTGCCAAGTTGGGTTACCTGGACCTTTGTAAAGACTACTTACCAGATAGAGAGC
  TTGTCTCCTCAGAATCCCCTCATCTCAAATTGCTTCAGCCCTTCCTCAGAACAGACTCAGGCATGCATGC
  CTTGATGGCCCATGAGCCCTCAGAAAGCGCAGGTGCCCAGCAGAACCCACAGCCCACCAAGTATTCTGTA
  GAGCAACTCAAAACATCAGAGTGTATTGTGCAGAGCAAACCAGTGGAGAGGCCCAGTGTGCAGTATGCCA
  AAGAGTTTGGTTACAGTCAGCAGTGTTTGCTCCCCAAGGCAAGACCAGAATTGACTAGTTCTGAAGGAGG
  CCTTCCTTTGCTACAGACACAGGGTCTGCAGTACACAGGCCCCTCTCCAGGGCTGGCTGTGGCGCCCCGT
  CAGCAACATGGCAGAACTCACCCCCTTAGAAGACTGAAAAGAGCAAATGATAAAAAACGGACAACCAACC
  CCTTCTATAACACCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291190
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291190.1, NP_001278119.1
• RefSeq Size: 9176 bp
• RefSeq ORF: 4290 bp
• Locus ID: 237860
• UniProt ID: Q5SW75
• Cytogenetics: 11 B5
• Gene Summary: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.