Vps8 (NM_001285893) Mouse Untagged Clone

CAT#: MC229587

Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (Vps8), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps8
• Synonyms: AI315068; AU040738; mKIAA0804
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229587 representing NM_001285893
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTGAACCAGACCAGGAGCATTTGGACCAGAACCCGTGCGCCAGGACTGTGGAAGAAGAGTTGA
  GCAAGTCTTTTAATTTAGAAGCTTCCCTTTCAAAATTCTCTTGCCTAGATCTGGATAAGGAGCTGGAGTT
  CAGAAGTGACCTGATCGATGACAAGGAGTTTGATATTCCTCAGGTGGATACCCCTCCAACTCTGGAAAGC
  ATTCTTAATGAGACGGACGATGAAGATGAGTCTTTCGTTCTGGAGGACCCTACCTTGTTAAACGTTGACA
  CCATCGACTCTCACTCTTACGATACCTCATCTGTGGCGAGCTCAGACAGCGGCGACAGGGCCAACCTAAA
  GAGGAAGAAGAAGTTGCCGGACTCTTTCTCGCTGCATGGGTCGGTTATGCGACATTCACTTCTAAAGGGG
  ATATCTGCACAGATCGTGTCCGCAGCCGACAAAGTAGATGCTGGCTTGCCTACAGCAATTGCCGTGTCCA
  GTCTGATAGCGGTGGGAACGTCTCACGGACTGGCTTTAATATTTGATCAGAATCAAGCACTGCGGCTCTG
  CCTGGGTAGCACCAGTGTTGGAGGGCAGTATGGAGCCATCTCTGCCCTCAGCATCAACAATGACTGCTCA
  AGACTTCTTTGTGGCTTTGCTAAAGGGCAGATCACCATGTGGGATTTGGCCAGTGGAAAACTTCTCAGAT
  CAATAACAGATGCTCATCCTCCAGGGACCGCAATCTTGCACATCAAGTTCACAGATGATCCAACTCTTGC
  GATTTGCAACGACAGTGGAGGCTCTGTTTTTGAACTAACATTTAAGAGAGTGATGGGCGTGCGAACGTGT
  GAGTCTCGGTGCCTATTCAGCGGCTCCAAGGGTGAGGTCTGCTGCATCGAGCCGCTGCACTCAAAGCCCG
  AGCTGAAAGACCATCCCATCACGCAGTTCTCACTGCTGGCCATGGCGTCCTTAACAAAGATATTGGTCAT
  TGGGTTGAAACCATCCCTGAAAGTGTGGATGACTTTCCCCTATGGACGGATGGATCCTTCCAGTGTTCCG
  CTGCTGGCCTGGCACTTCGTGGCAGTGAATAACTCTGTGAATCCCATGCTCGCCTTCTGCAGAGGCGACA
  TGGTTCACTTTCTCCTGGTTAAGAGAGATGAGTCGGGCGCAATACACGTCACCAAGCAAAAGCACCTTCA
  CCTGTACTATGACCTGATCAACTTCACTTGGATCAACTCGCGCACAGTCGTGCTTTTAGACAGCGTGGAG
  AAGCTGCACGTGATTGATCGGCAGACGCAGGAGGAACTGGAGACAATGGAGATCTCGGAAGTCCAGCTGG
  TCTACAACAGCAGCCACTTCAAGTCATTGGCCACGGGAGGAAATGTTAGCCAGGCCCTGGCTTTGGTTGG
  AGAGAAGGCTTGCTACCAATCCATCAGTAGCTATGGAGGACAGATCTTCTATCTGGGGACCAAGTCTGTG
  TACGTGATGATGCTGAGAAGCTGGAGGGAGAGGATGGACCATCTCCTGAAACAGGATTGCCTTACGGAAG
  CCTTGGCCCTCGCGTGGTCTTTTCATGAAGGAAAGGCAAAAGCAGTAGTGGGGCTATCGGGGGATGTCAG
  CAAGAGGAAGGCTGTTGTTGCTGATCGGATGGTAGAGATCCTGTTCCATTATGCAGATCGGGCTCTGAAA
  AAGTGCCCGGACCAGGGGAAAATCCAAGTGATGGAGCAACACTTTCAGGATACGGTGCCAGTCATAGTGG
  ACTACTGCCTTCTGCTCCAGCGAAAGGATCTTCTATTTGGTCAAATGTACGACAAATTAAGTGAAAATTC
  CGTCGCAAAAGGCGTGTTTTTGGAGTGCCTTGAGCCATATATCTTAAGTGATAAACTGGTGGGGATTACT
  CCCCAGGTCATGAAGGACTTAATTGTTCATTTCCAAGATAAAAAACTGCTGGAGAATGTGGAAGCCCTCA
  TTGTCCACATGGACATCACCAGCCTCGACATTCAGCAGGTGGTCCTCATGTGTTGGGAGAATCGTCTGTA
  TGACGCCATGGTCTATGTCTACAACCGAGGCATGAATGAGTTTATCAGTCCGATGGAGAAGCTTTTCAAA
  GTCATCGCCCCACCTCTGAATGCAGGGAAGACTCTTACAGATGAGCAAGTTGTTATGGGCAACAAGCTTC
  TGGTGTACATCAGCTGTTGCCTGGCAGGTCGTGCCTACCCTCTCGGTGACATTCCTGAAGATCTTGTTCC
  CTTGGTTAAAAACCAGGTTTTTGAGTTTCTGATTCGCCTGCATTCTGTAGAAGCATCCTCCGAGGAAGAA
  GTCTATCCTTATGTTCGGACTTTGCTACATTTTGACACAAGAGAATTTCTGAATGTGCTGGCACTGACCT
  TTGAAGACTTTAAAAATGATAAGCAAGCTGTGGAATACCAGCAGCGCATTGTGGATATTTTATTGAAGGT
  TATGGTGGAGAATTCAGATTTTACCCCGTCACAAGTGGGGTGTCTCTTTACGTTCCTCGCTCGTCAGCTC
  GCAAAGCCTGACAATACCTTGTTTGTGAACAGGACCCTCTTTGATCAAGTCCTCGAGTTCCTCTGTAGCC
  CCGACGATGACTCCCGCCACTCTGAAAGACAGCAGGTCCTTTTAGAATTGCTGCAGGCCGGAGGCATAGT
  TCAATTTGAAGAGAGTCGGCTCATCCGCATGGCAGAAAAGGCAGAATTCTATCAAATCTGTGAATTTATG
  TATGAGCGGGAACACCAGTATGACAAGATCATTGACTGCTACCTGCACGACCCATTGAGAGAGGAAGAAG
  TCTTCAATTATATCCACAACATCTTATCTATTCCTGGTCACAGTGCTGAGGAGAAGCAGTCTGTGTGGCA
  GAAAGCAATGAATCATATGGAGGAACTTGTGTCCCTGAAGCCCTGCAAAGCCGCAGAGCTGGTGGCTACT
  CACTTTTCTGAGCAGATCGAAGTGGTCATTGGACAGCTTCAGAACCAGCTTTTGCTTTTCAAATTTTTGA
  GGAGTCTTCTTGATCCAAGGGAAGGTGTTCATGTCAATCAGGAGTTGCTGCAGATCCCGCCTCACATTAC
  AGAGCAGTTCATTGAGCTGCTGTGCCAGTTCAGTCCTGACCAAGTCATACAGACACTGCAGGTCCTTGAG
  TGCTACCGTCTGGAGGAAACGATTCAGATTACACAAAAGTATCAACTCCATGAAGTCACTGCGTATCTGC
  TGGAGAAGAAAGGGGATGCACACGGCGCCTTCCTGCTGCTGCTGGAGAGACTGCAGAGCAGGCTGCAAGA
  GATGACACGGCAGGACGAAAATACCAAGGAGGACATCCTGCTGAAAGGTGTTGAAGATACCATGGTAGAG
  ACAATTGCTCTTTGCCAGAGAAATTCACAGAATTTGAATCAGCAGCAACGAGAGGCTCTATGGTTTCCAC
  TGTTGGAGGCAATGATGACACCACAGAAGCTGTCCAGCTCGGCTGCTGCTCCTCATCCGCACTGTGAAGC
  TCTGAAGTCTTTGACCATGCAAGTCCTAAACAGCATGGCAGCGTTCATCGCCCTCCCGTCCATCCTACAG
  AGAATCTTGCAGGACCCAATTTATGGAAAAGGAAAGCTTGGAGAAATCCAGGGCCTTATTCTGGGGATGC
  TGGACACCTTCAACTATGAGCAAACCTTGCTAGAAACAACAGCCAGCCTCCTGAACCAAGATCTCCATTG
  GTCACTGTGTAACCTGAGAGCATCAGTATCCAGAGGACTCAATCCCAAGCAGGATTACTGTTCTATATGT
  TTACAGCAGTACAAAAGACGCCAAGAAATGGCTGATGAAATTATTGTCTTTAGCTGTGGCCATTTGTATC
  ATTCATTCTGTCTCCAAAGTAAGGAATGCACCCTAGAGGTTGAGGGTCAGACGAGATGGGCGTGTCACAA
  ATGCAGCTCAAGTAATAAAGCGGGCAAACTCAGTGAAAATCCTTCTGAAAACAAGAAAGGACGGATAACC
  TCGTCTCAGGTAAAAATGTCGCCCTCGTATCATCAGTCCAAAGGGGATCCTCCCGCCAGGAAGGCAAACT
  CAGAACCTGTTCTGGACCCACAGCAAATGCAAGCCTTTGATCAGCTCTGCCGTCTCTACAGAGGAAGTTC
  TAGGTTGGCTCTCCTTACGGAGCTCTCCCAGAACCGAGGTGGCGACAGCTGCAGGCCGTTTGCTGGCCCC
  CAGAGTGGGCCTGCTTTCAACAGCGTCTTCCAGAAGGAGAACTTCCAGCTGCAGCTCGCGCCTCCGCCTG
  TGGCTGAAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285893
• Insert Size: 4284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285893.1, NP_001272822.1
• RefSeq Size: 5027 bp
• RefSeq ORF: 4284 bp
• Locus ID: 209018
• UniProt ID: Q0P5W1
• Cytogenetics: 16 B1
• Gene Summary: Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.