Lmtk3 (NM_001290990) Mouse Untagged Clone

CAT#: MC229586

Lmtk3 (untagged) - Mouse lemur tyrosine kinase 3 (Lmtk3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lmtk3
• Synonyms: aatyk3; BC059845
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229586 representing NM_001290990
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCACCCGGCGCACTCATCCTCCTGGCGGCCGTCTCCGCCTCCGGCTGCCTGGCGTCCCCAGCGC
  ACCCGGATGGATTCGCTCTGAGCCGGGCCCCTTTGGCTCCGCCCTACGCTGTGGTCCTCATTTCCTGTTC
  GGGTCTGCTGGCCTTCATCTTTCTCCTCCTTACCTGTCTGTGTTGCAAACGGGGTGATGTTCGTTTCAAG
  GAGTTTGAGAACCCGGAAGGGGAGGACTGCTCTGGGGAGTACACCCCCCCTGCGGAGGAGACCTCCTCCT
  CACAGTCGCTGCCTGATGTCTATATTCTGCCGCTGGCAGAGGTCTCACTGCCAATGCCTGCCCCGCAGCC
  TCCACACTCAGACATCAGCACCCCCCTGGGCCTGAGCCGCCAGCACCTCAGCTACCTGCAGGAGATTGGC
  AGCGGCTGGTTTGGGAAGGTGATCCTCGGGGAGGTTTTCTCAGACTACTCGCCAGCCCAGGTGGTGGTGA
  AGGAACTCCGGGCTAGTGCCGGGCCCCTGGAACAGCGCAAGTTCATCTCCGAGGCTCAGCCCTACAGGAG
  CCTGCAGCATCCCAACGTCCTCCAGTGCCTGGGCGTCTGTGTGGAGACCTTGCCCTTCCTGTTGATCATG
  GAGTTCTGCCAGCTGGGGGACCTGAAGCGATACCTTCGGGCCCAGCGGCCACCTGAAGGCATGTCCCCTG
  AACTTCCCCCTCGAGACCTTCGGACACTGCAGAGGATGGGTCTAGAGATTGCCCGAGGACTGGCACACCT
  GCACTCTCACAACTATGTGCACAGCGATCTGGCGCTGCGCAACTGCCTGCTAACTTCAGACCTGACTGTG
  CGTATTGGAGACTATGGGCTGGCGCACAGCAACTACAAGGAAGACTACTACCTGACACCCGAGCGCCTAT
  GGGTGCCGCTGCGCTGGGCAGCGCCCGAGCTGCTGGGCGAGCTGCACGGCAGCTTCGTGCTGGTAGATCA
  AAGCCGGGAGAGTAATGTCTGGTCCCTGGGGGTGACACTCTGGGAGCTATTCGAGTTCGGGGCACAGCCC
  TATCGTCACCTATCGGATGAGGAGGTCCTGGCCTTTGTTGTCCGCCAGCAGCACGTGAAGCTGGCCCGGC
  CCAGGCTCAAGCTGCCCTACGCTGACTATTGGTATGACATTTTGCAGTCTTGCTGGCGGCCGCCAGCCCA
  GCGCCCCTCAGCTTCTGATCTCCAGCTGCAGCTCACTTACCTGCTGTCTGAGCGGCCCCCCAGGCCCCCT
  CCTCCACCACCCCCTCCCCGAGATGGGCCCTTCCCCTGGCCCTGGCCCCCCTCGCATAGTGCGCCGCGCC
  CAGGGACCCTGTCCTCCCAGTTCCCCCTTCTGGATGGCTTCCCCGGGGCTGACCCTGATGATGTACTCAC
  AGTCACCGAGAGCAGCCGTGGCCTCAACCTTGAGTGCCTGTGGGAGAAGGCCCGCCGTGGGGCAGGCCGG
  GGTGGGGGTGCACCTCCCTGGCAACCCGCTTCTGCGCCTCCTGCGCCCCATACCAATCCATCCAATCCCT
  TCTATGAGGCGCTGTCCACCCCTAGCGTGCTTCCGGTCATCAGCGCACGCAGTCCCTCGGTGAGCAGCGA
  GTACTATATCCGCCTGGAGGAGCACGGTTCTCCACCAGAGCCCCTCTTCCCCAACGACTGGGACCCGCTG
  GACCCAGGAGTACCCGGTCCCCAGGCCCCCCAGACTCCCTCCGAGGTCCCTCAGCTGGTGTCCGAGACCT
  GGGCTTCCCCCCTCTTCCCCGCGCCCCGACCCTTCCCGGCCCAGTCCTCGGGATCAGGTGGTTTCCTGCT
  GAGCGGCTGGGACCCCGAGGGCCGGGGCGCAGGAGAGACCCTGGCAGGAGATCCTGCCGAGGTGCTTGGG
  GAACAGGGTACCGCACCCTGGGCCGAAGAGGAGGAGGAAGAGAGCTCCCCAGGCGAGGACAGCAGCAGCC
  TTGGAGGGGGACCCAGCCGCAGGGGACCCCTCCCCTGTCCCTTGTGCAGCCGCGAGGGTCCCTGCTCCTG
  TCTGCCATTGGAGCGGGGGGACGCTGTGGCCGGCTGGGGGGACCACCCTGCCCTTGGTTGTCCCCACCCC
  CCAGAGGACGACTCTTCCTTGCGTGCAGAGCGGGGCTCCTTGGCCGACCTGCCTCTGGTCCCCCCTACCT
  CAGCCCCTCTCGAGTTTCTGGACCCCCTTATGGGGGCCGCAGCGCCCCAGTACCCCGGGCGGGGGCCACC
  TCCCGCTCCCCCCCCTCCACCGCCCCCTCCCCGGGCCTCCGCGGAACCGGCCGCGTCCCCCGACCCCCCG
  TCGGCCCTGGCCAGTCCAGGCTCCGGCCTATCGTCTCCGGGCCCCAAGCCGGGGGACAGCGGCTACGAGA
  CCGAGACCCCTTTTTCCCCCGAGGGAGCCTTCCCAGGTGGGGGGGCGGCCGAGGAGGAAGGGGTCCCTCG
  GCCACGGGCTCCCCCCGAGCCACCCGACCCAGGAGCGCCCCGGCCACCCCCAGACCCGGGTCCCCTCCCA
  CTCCCAGGGTCCCAGGAGAAGCCAACCTTTGTAGTTCAGGTGAGCACCGAGCAGCTGCTGATGTCCCTAC
  GGGAGGATGTGACAAAGAACCTCCTAGGGGACAAGGGGTCGACACCCGGGGAGACAGGACCCAGGAAGGC
  GGGGAGAAGCCCCGCGAACAGAGAGAAGGGCCCAGGCCCGAACAGGGACCTGACATCCCTGGTCAGCAGG
  AAGAAAGTCCCCAGCAGGAGCCTTCCAGTGAACGGGGTGACAGTGTTGGAGAACGGGAAGCCAGGAGTCC
  CGGACATGAAGGAGAAGGTGGCGGAGAATGGCCTGGAATCTCCGGAGAAAGAAGAGAGAGCCCTGGTGAA
  TGGGGAGCCGATGTCCCCAGAGGCCGGGGAGAAGGTGCTGGCGAATGGGGTTCTGATGTCCCCAAAGAGC
  GAGGAGAAGGTGGCAGAGAATGGGGTCCTGAGGCTGCCCAGGAACACGGAGAGGCCACCAGAGATTGGAC
  CTCGGAGAGTCCCAGGGCCCTGGGAGAAGACGCCCGAGACTGGGGGTCTAGCTCCCGAGACCCTGCTGGA
  TCGAGCCCCTGCGCCCTGCGAGGCAGCCTTGCCCCAGAACGGCTTGGAGATGGCCCCTGGCCAGCTTGGC
  CCAGCCCCCAAGAGCGGGAACCCAGACCCCGGGACCGAGTGGAGAGTCCACGAGAGTGGGGGGGCACCGA
  GAGCCCCCGGGGCTGGGAAGCTGGACCTCGGGAGTGGGGGCCGAGCCCTGGGGGGCGTGGGGACGGCCCC
  CGCCGGCGGCCCCGCAAGCGCCGTGGACGCAAAGGCCGGATGGGTAGACAACTCGAGACCACTGCCACCT
  CCGCCACAGCCACTGGGGGCCCAACAGAGGAGGCCGGAGCCAGTGCCCCTGAAAGCCAGGCCGGAGGTGG
  CCCAAGAGGAAGAGCCAGGGGTCCCAGACAACAGGCTCGGCGGAGACATGGCCCCCAGCGTAGACGAGGA
  CCCCCTCAAGCCGGAGAGGAAGGGCCCCGAGATGCCACGCTTGTTCCTGGACTTGGGACCACCTCAGGGG
  AACAGCGAGCAGATCAAAGCCAAACTCTCCCGGCTCTCGCTGGCGCTCCCACCGCTCACGCTCACGCCGT
  TCCCGGGCCCGGGCCCGCGGCGGCCACCTTGGGAGGGCGCGGACGCAGGGGCAGCTGGCGGGGAGGCCGG
  CGGGGCGGGGGCGCCGGGGCCAGCGGAGGAGGACGGGGAGGACGAGGACGAGGACGAGGAGGACGAGGAG
  GCAGCGGGCTCTCGGGATCCCGGGAGGACGCGGGAAGCCCCAGTGCCCGTCGTGGTGAGCAGCGCCGACG
  GGGACACGGTCCGCCCGCTGCGGGGGCTGCTCAAGTCTCCACGCGCGGCCGACGAGCCAGAGGACAGCGA
  GCTGGAGAGGAAGCGCAAGATGGTCTCCTTCCACGGGGACGTGACCGTCTACCTCTTCGACCAGGAGACT
  CCAACCAACGAGTTGAGCGTCCAGGGCACCCCCGAGGGGGACACGGAGCCATCAACTCCCCCAGCGCCCC
  CGACGCCTCCCCACCCCACCACCCCAGGAGATGGGTTTCCCAACAGCGACAGCGGCTTTGGCGGCAGTTT
  CGAGTGGGCGGAGGATTTCCCCCTCCTCCCCCCGCCAGGCCCCCCCCTGTGCTTCTCCCGCTTCTCCGTC
  TCACCTGCACTGGAGACCCCGGGGCCTCCCGCCCGGGCTCCCGACGCCCGGCCCGCAGGCCCCGTGGAGA
  ACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290990
• Insert Size: 4275 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290990.1, NP_001277919.1
• RefSeq Size: 5022 bp
• RefSeq ORF: 4275 bp
• Locus ID: 381983
• UniProt ID: Q5XJV6
• Cytogenetics: 7 B3
• Gene Summary: Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (By similarity). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (PubMed:24760852).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same protein.