Erbin (NM_001289475) Mouse Untagged Clone

CAT#: MC229583

Erbb2ip (untagged) - Mouse Erbb2 interacting protein (Erbb2ip), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erbin
• Synonyms: 1700028E05Rik; Erbb2ip; mKIAA1225
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229583 representing NM_001289475
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAAAACGAAGTTTGTTTGTGCGGTTGGTACCATGTCGCTGTCTACGAGGAGAAGAGGAGACAG
  TCACTACTCTAGATTATTCTCATTGCAGCTTGGAACAAGTTCCCAAAGAGATTTTCACCTTTGAAAAAAC
  TTTAGAGGAGCTCTATTTAGATGCTAATCAGATTGAAGAGCTTCCAAAGCAACTTTTTAATTGTCAGTCT
  CTACATAAACTAAGTTTGCCAGACAATGATTTAACAACATTACCAGCCTCCATTGCAAATCTTATTAATC
  TCAGGGAACTGGATGTCAGCAAAAATGGAATACAAGAATTTCCAGAAAATATTAAAAATTGTAAAGTTTT
  GACAATTGTGGAGGCTAGTGTGAACCCTATTTCTAAGCTCCCTGATGGGTTTTCTCAGCTGCTAAACCTA
  ACCCAGTTATATCTGAATGATGCTTTCCTTGAATTCTTACCAGCTAATTTTGGCAGATTAACTAAACTCC
  AGATTCTAGAACTTAGAGAAAACCAGTTGAAAATGTTGCCTAAAACCATGAATAGACTGACCCAGCTGGA
  AAGGCTGGATTTGGGAAGTAATGAATTCACAGAAGTGCCTGAAGTACTTGAACAACTAAGTGGATTGAGA
  GAGTTTTGGATGGATGGCAATAGACTGACTTTTATTCCAGGGTTTATTGGTAGTTTGAGACAGCTCACGT
  ACTTGGATGTTTCTAAAAATAATATTGAAATGGTTGAAGAAGGAATTTCAACATGTGAAAACCTCCAGGA
  CTTTCTCTTATCAAGTAATTCCCTTCAGCAGCTGCCGGAAACTATTGGTTCATTGAAAAATGTCACAACT
  CTAAAAATTGATGAAAACCAATTAATGTATCTACCAGACTCAATAGGAGGGTTAAGGTCAATAGAAGAAC
  TGGATTGCAGTTTCAATGAAATTGAAGCTTTGCCTTCCTCAATTGGCCAGCTCACAAACATGAGAACATT
  TGCTGCAGATCACAATTACTTACAACAGCTACCCCCAGAGATTGGAAACTGGAAAAATATAACTGTGCTG
  TTTCTCCATTGTAATAAACTAGAGACGCTTCCAGAGGAAATGGGTGATATGCAAAAATTAAAAGTCATCA
  ATTTAAGTGATAATAGGTTAAAGAATTTGCCTTTTAGCTTTACAAAGCTACAACAGTTGACTGCTATGTG
  GCTGTCAGATAATCAGTCCAAACCTCTGATACCTCTTCAAAAAGAAACAGACACAGAGACTCAGAAAATG
  GTGCTTACTAATTACATGTTCCCTCAGCAGCCACGGACTGAAGACGTTATGTTCATATCAGATAATGAAA
  GTTTTAATCCTGCATTGTGGGAAGAACAGAGAAAACAGCGGGCTCAAGTGGCATTTGAATGTGATGAAGA
  CAAAGATGAAAGAGAGGCACCACCCAGGGAGGGAAACTTAAAGAGATATCCAACACCATACCCAGATGAG
  CTTAAGAATATGGTCAAGACTGTTCAAACCATTGTACATAGATTAAAAGATGAAGAGACTAATGAAGAGT
  CTGGGAGAGATTTGAAGCAGCATGAAGATCAGCAAGTTGTAAATAAGGATAAGTGTGTGAAGACTTCAGA
  AAGTACTACAACAAAAAGCAAACTTGATGAAAGAGAAAAGTATATGAATTCTGTACAGAAAATGAGTGAG
  CCTGAAGCTGAGACCAATGGTGGAAATTTGCCAGTAACTGCAAGTATGAAGTTGTCTGGAAACCTGAAGC
  ATATTGTTAACCATGATGATGTTTTTGAGGAATCTGAAGAACTTTCTTCTGATGAAGAAATGAAAATGGC
  AGAGATGCGACCTCCATTAATTGAATCCTCGATTAATCAGCCCAAAGTTGTAGCACTTAGTAATAATAAA
  AAGGATGATGCGAAGGATGCAGACTCTTTATCCGATGAAGTCACACACAACAGTAATCAGAATAACAGCA
  ACTGTTCTTCTCCGTCCCGAATGTCTGATTCAGTGTCTCTTAATACTGACAGCAGTCAAGACACGTCACT
  CTGTTCTCCCGTGAAACAGACTCCCGTGGATAGTAACTCCAAAGTCAGACAAGAAGATGAAAATTTTAAC
  AGCCTTTTACAAAATGGAGTTAATTTAAACAATTCACCAGAGGAAAAATTCAAAATTAATGATAAAAAGG
  ATTTTAAGTTACCTGAGTATGATTTGAATATTGAAGAACAGTTAGTTCTGATTGAAAAAGACATTGATTC
  AAAAGCCACATCTGATGACTCTCGCCAGTTAGACCATATCAACATGAATATTAATAAACTTGTGACCAAT
  AATATTTTTCAACCAGAAGTAATGGAAAGATCAAAGATGCAGGATATAGTACTTGGAACAGGCTTTTTAA
  GTATTCATCCTAAAAATGAGGCTGAGCACATAGAAAATGGGGCTAAGTTTCCGAATTTGGAGTCCATAAA
  TAAGGTAAATGGTCTTTGTGAGGACACTGCACCGTCTCCTGGTAGGGTTGAACCACAGAAGGCCAGTTCT
  TCTGCTGACGTGGGCATTTCTAAAAGCACGGAAGATCTATCTCCTCAGAGAAGTGGTCCAACTGGAGCTG
  TTGTGAAATCTCATAGTATAACTAACATGGAGACTGGAGGTTTAAAAATCTATGACATTCTTGGTGATGA
  TGGCCCTCAGCCGCCAAGTGCAGCAGTTAAAATCGCATCTGCTGTGGATGGGAAGAACATAGTCAGAAGC
  AAGTCTGCCACACTGCTGTATGATCAGCCCCTGCAGGTGTTCACTGCTGCCTCCTCATCTTCTGAGTTAC
  TGTCAGGTACAAAGGCAGTTTTCAAGTTTGATTCAAATCACAACCCTGAAGAGCCAGATATAATAAGAGC
  TGCCACAGTATCTGGCCCACAGTCTACACCTCACCTGTATGGTCCCCCACAATATAATGTCCAGTACAGT
  GGCAGTGCTACAGTGAAAGACACCTTGTGGCACCCTAAACAGAATCCACAAATAGATCCTGTCAGTTTTC
  CTCCTCAGCGCCTTCCAAGATCTGAGAGTGCAGAAAATCACAGTTATGCAAAGCATTCTGCCAACATGAA
  TTTCTCTAACCATAACAATGTTAGAGCCAATACTGGATACCACTTACAACAGAGACTTGCCCCAGCCAGG
  CATGGAGAAATGTGGGCTATCTCCCCGAATGACCGACTAGTGCCAGCAGTCACTCGGACTACTATTCAGA
  GACAGAGTAGCGTGTCTTCAACGGCTTCTGTAAATCTCGGTGACCCCACAAGGCGTACTGAAGGAGATTA
  CTTATCGTACAGAGAGTTACATTCAATGGGAAGAACTCCAGTCATGTCAGGATCACAGAGACCTCTTTCT
  GCACGAGCGTACAGCATCGATGGCCCAAATACATCCAGGCCTCAGAGTGCCCGTCCCTCTATTAATGAAA
  TACCAGAGAGAACTATGTCAGTTAGTGATTTCAATTACTCACGGACTAGTCCTTCAAAAAGACCAAATAC
  AAGGGTCGGGTCTGAACATTCTCTGTTAGATCCTCCAGGAAAAAGCAAGGTTCCTCATGACTGGCGGGAA
  CAAGTACTACGACACATTGAGGCCAAAAAGTTAGAAAAGAGTATGCTGTCAAGGTCCTTTAATTCCAATC
  TTACTGCTGTAAGCAGTTCTCACTATGGCAGCTCTAGGGATCTGCATGGCAGCCAGGGCAGCCTTGCCCT
  GAGTGTTGCAGACGGAAGAGGTTCTGGTGGGCACATTTTTCGAATGCCTTTGAGTAATGGACAAATGGGC
  CAGCCTCTCAGGCCTCAGGCACATTATAGTCAAACCCATCACCCCCCTCAGGCATCTGTGGCAAGGCATC
  CCTCTAGAGAACAACTAATTGATTATTTGATGCTGAAAGTGGCCCACCAGCCTCCATATACACATCCCCA
  TTGTTCTCCTAGACAAGGCCATGAACTGGCAAAGCAAGAGATTCGAGTAAGAGTAGAAAAGGATCCAGAA
  CTTGGATTTAGCATATCAGGAGGTGTCGGCGGCAGAGGAAACCCTTTCAGACCTGATGATGATGGTATAT
  TTGTAACAAGGGTACAACCTGAAGGACCAGCATCAAAATTACTACAGCCAGGAGATAAAATTATTCAGGC
  TAATGGCTACAGTTTTATCAACATTGAACATGGGCAAGCGGTGTCCTTGTTAAAAACTTTCCATAATGCA
  GTAGATCTCATCATTGTACGAGAAGTTTCTTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289475
• Insert Size: 4236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289475.1, NP_001276404.1
• RefSeq Size: 6557 bp
• RefSeq ORF: 4236 bp
• Locus ID: 59079
• UniProt ID: Q80TH2
• Cytogenetics: 13 D1
• Gene Summary: Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated ERBB2 'Tyr-1248' receptor, it may contribute to stabilize this unphosphorylated state (By similarity). Inhibits NOD2-dependent NF-kappa-B signaling and proinflammatory cytokine secretion (PubMed:16203728).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 5), compared to isoform 1.