Usp47 (NM_177249) Mouse Untagged Clone

CAT#: MC229572

Usp47 (untagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp47
• Synonyms: 4930502N04Rik; A630020C16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229572 representing NM_177249
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAGGAGGATGTGTTTTGGAGATGCAGACAAAATATCT
  TTGATGAAATGAAGAAGAAATTTTTACAGATAGAAAATGCTGCTGAAGAACCTAGAGTCTTATGTATAAT
  ACAAGACACTACTAACTCGAAGACAGTGAGTGAACGGATCACATTAAATTTACCAGCATCTACCCCAGTC
  CGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGATTTGACCAGGGAAA
  ATGGAGTCACTACTGCTGACATGGCACCACTGGATCACACCAGTGACAAGTCTCTTCTTGATGCTAATTT
  TGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACCGCCTCAGATGTTGTTGGAG
  GACTCCAATAACGTGGATGACAGCGTGCATGACAGGTTCATAGGTCCACTGCCAAGAGAAGGTTCTGTGG
  CCTCTACCAATGATTATGTCAGCCAGAACTACTCCTATTCATCAATTTTGAACAAGTCAGAAACTGGTTA
  TGTGGGATTAGTAAACCAGGCGATGACGTGCTATTTGAATAGCCTTTTGCAAACACTTTTTATGACTCCT
  GAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGACTCTGAAGAAGATCCTGTAACGAGTATCCCAT
  ACCAGCTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAGACCACAGATGTTAC
  AAGGAGCTTTGGATGGGATAGCAGTGAGGCTTGGCAACAGCATGATGTACAAGAACTGTGCCGTGTTATG
  TTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTGTACCAAGGCA
  AGCTGAAAGATTATGTGAGGTGTCTGGAATGCGGTTATGAAGGCTGGAGAATTGACACGTATCTTGATAT
  TCCACTGGTCATCCGACCTTATGGGTCCAGCCAAGCGTTTGCTAGTGTGGAAGAAGCGTTGCATGCATTT
  ATTCAACCAGAGATCTTGGATGGCCCAAATCAATATTTTTGTGAACGTTGTAAGAAGAAGTGTGATGCTC
  GGAAGGGCCTTCGGTTTTTGCATTTCCCCTATCTGCTGACCTTACAGTTGAAAAGGTTTGATTTTGATTA
  TACAACCATGCATAGAATTAAACTGAATGATCGAATGTCATTTCCGGAGGAGCTGGATATGAGCACATTT
  ATTGATATTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGATAGTGGAGCAGAAAATGAAGGCA
  GTTGCCACAGCGACCAGATGAGTAATGATTTCTCCACGGATGATGCTGTTGATGAAGGGATATGCCTGGA
  GAGCAGTAGTGGGAGTGAGAAGATTTCAAAACCTGGCCTGGAAAAGAACTCCCTGATGTATGAGCTCTTC
  TCAGTGATGGTGCATTCAGGGAGTGCTGCTGGAGGACACTATTATGCTTGCATAAAGTCTTTCAGTGACG
  ATCAATGGTACAGTTTCAATGACCAACATGTGAGCAGGATAACACAAGAAGACATTAAGAAGACACATGG
  TGGGTCTTCAGGAAGCAGAGGCTACTACTCCAGTGCTTTTGCAAGCTCAACAAATGCATATATGCTGATA
  TACAGACTGAAGGATCCAACAAGAAATGCAAAGTTTCTAGAAGTGGACGAGTACCCAGAACACATTAAGA
  ATCTGGTGCAGAAGGAGAGAGAGCTGGAAGAGCAGGAAAAGAGGCAACGAGAGATTGAACGCAACACGTG
  CAAGATAAAATTATTTTGTTTGCATCCTGTGAAACAAGTAATGATGGAAAACAAGCTGGAGGTTCATAAG
  GATAAGACGCTAAAGGAAGCCGTTGAAATGGCTTATAAGATGATGGATTTAGAAGATGTGATTCCCTTGG
  ATTGCTGTCGCCTGGTAAAATATGACGAGTTTCATGATTACCTGGAACGATCGTATGAGGGAGAAGAAGA
  TACACCAATGGGGTTGCTTCTAGGAGGAGTCAAGTCCACATACATGTTTGATCTGCTGTTGGAAACGAGA
  AAGCCTGATCAAATTTTTCAGTCTTACAAACCTGGAGAAGTGATGGTGAAAGTTCATGTTGTTGATCTGA
  AAGCAGAGACTGTCGCTGCTCCTGTAACAGTGCGTGCGTATTTAAATCAGACAGTTACAGAGTTTAAACA
  GCTGATCTCAAAGGCCACGCACTTACCTGCAGACTCCATGAGGATAGTGCTGGAGCGCTGCTACAATGAC
  CTGCGCCTTCTCAGCATGCCCAGCAAGACCCTGAAGGCTGAGGGCTTCTTCAGAAGTAACAAGGTGTTTG
  TTGAAAGCTCTGAGACAGTGGATCACCAGGCAGCCTTTACAGACTCCCACTTGTGGAAACTCCTGGATCG
  ACATGCAAATACAATCCGGTTATTTGTTTTGCTGCCTGAACAATCTCCAGGATCATATTCGAAAAGGACA
  GCATACCAGAAAGCTGGAGGTGATTCTGGTAATGTGGACGATGATTGTGAAAGAGTCAAAGGACCTGCAG
  GGAATTTAAAGTCTGTGGATGCTATTCTAGAAGAGAGCACTGAAAAACTGAAAAGCTTGTCACTGCAGCA
  GCAGCAGCAAGATGGGGACAACGGAGACAGCAGCAAAAGCACTGAGACAAGTGACTTTGAAAATATTGAA
  TCCCCTCTCAATGAGAGGGGCTCTTCCACCTCTGTGGATAATCGAGAACTTGAACAGCACATTCAGACTT
  CTGACCCAGAAAACTTTCAGTCTGAGGAACGCTCGGACTCAGATGTGAATAATGACCGGAGTACAAGTTC
  GGTAGACAGTGATATTCTTAGCTCCAGTCACAGCAGTGACACTTTGTGCAATGCAGACAGCGCTCAGATT
  CCTCTGGCCAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACTAAAGCAAACGAGGGGAAAA
  AGGAGACCTGGGACACAGCTGAAGAAGACTCTGGGACTGACAGTGAATATGACGAGAGTGGCAAGAGCAG
  GGGGGACATGCAGTACATGTACTTCAAAGCTGACCCCTACACTGCGGATGAAGGCTCTGGGGAAGGACAC
  AAATGGTTGATGGTGCATGTTGATAAGAGAATCACCCTGGCAGCTTTCAAACAGCATTTAGAACCTTTTG
  TTGGAGTCCTCTCCTCTCACTTCAAGGTTTTTCGAGTGTACACCAGCAATCAAGAGTTTGAGACCGTCCG
  GTTGAATGAGACCCTTTCATCATTTTCAGATGACAATAAGATCACAATCCGACTGGGGAGAGCACTGAAG
  AAGGGAGAGTACAGAGTTAAAGTGTGCCAGCTCTTAGTCAATGAGCAAGAGCCATGCAAGTTTCTGCTAG
  ACGCTGTGTTTGCTAAAGGCATGACTGTGCGGCAATCAAAAGAGGAGCTGATTCCTCAACTCAGGGAGCA
  GTGTGGCTTAGACCTCAGCATCGACAGGTTTCGTCTAAGGAAGAAAACATGGAAGAATCCTGGCACTGTC
  TTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAAGTTTTCCTGGAAGTTC
  TTGATGGTGTGGAGAAGATGAAGTCCATGTCACAGCTTGCCATCTTAACAAGACGCTGGAGGCCTGCAGA
  GATGAAGTTGGACCCCTTCCAGGAGCTTGTGTTGGAAAGCAATAGTGTTGATGAACTCCGAGAGAAGCTA
  AGTGAAATCAGTGGGATTCCTTTGGAAGACATTGAATTTGCCAAGGGTAGGGGAACCTTCCCCTGTGATA
  TTTCTGTCCTTGATATTCATCAAGATTTGGACTGGAATCCTAAAGTCTCTACCCTGAATGTTTGGCCTCT
  TTATATCTGTGATGATGGTGCCGTCATATTTTATAGGGATAGAACAGAAGAAGTGATGGAATTGACAGAT
  GAGCAAAGAAATGAATTGATGAAAAAGGAAAGCAGCCGACTGCAGAAGACGGGACATCGTGTGACATACT
  CCCCTCGCAAAGAGAAAGCACTCAAGATCTACCTGGATGGGGCGCCAAATAAAGATGTGGCTCAAGACTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177249
• Insert Size: 4131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177249.3, NP_796223.2
• RefSeq Size: 5600 bp
• RefSeq ORF: 4131 bp
• Locus ID: 74996
• UniProt ID: Q8BY87
• Cytogenetics: 7 F1
• Gene Summary: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode protein isoforms of identical length but with distinct amino acid sequences.