Tcof1 (NM_001198984) Mouse Untagged Clone

CAT#: MC229570

Tcof1 (untagged) - Mouse Treacher Collins Franceschetti syndrome 1, homolog (Tcof1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcof1
• Synonyms: AA408847; AW209012; treacle
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229570 representing NM_001198984
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTCATTTACCATCATCTGTTGCAAGCAGGCTACG
  TCCGCGCGGCGCGGGAAGTAAAGGAGCAGAGCGGCCAGAAGAGTTTCCTGACTCAGCCCGTCACCCTTCT
  GGACATCTATACACACTGGCAACAGACCTCAGAGCTTGGCCAGAAGCAGAAGGCAGAGGATGATGAGACC
  CTTCAGGCTAAGAAGTCTCGAGTGTCGGATCCTGTTAGCAGCTCAGAGAGCTCGGATCAGGAGAAGGAAG
  AGGAGGCAGCAACCGAAAGGGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCTGCAACCGCAGCTTT
  GCCATCAAAAGTAAAAGAAAAGGGAAAGACCAAGACAGCCAACAAGACGGTGAACTCTGTGTCGCACCCT
  GGGTCCGGAAAGACGGTGGTCCACCTGCTCTCTGGGAAGTCACCCAAAAAGTCAGCAGAGCCCTTGGCAA
  ACACTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAATGCCCAAGCCCTCGGACCCACTGCCAAGTCTGG
  AACGGTGTCAGCGGGCCAAGGCAGCAGTTCCAGTGAAGATTCCTCCATCTCAAGCGATGAGACAGATGTC
  GAGGTGAAATCTCCAGCAAAACCAGCCCAGGCCAAAGCTTCAGCAGCCCCTGCCAAGGATCCTCCAGCAA
  GAACAGCCCCAGGCCCTACCAAGTTAGGGAATGTGGCGCCCACACCTGCTAAACCAGCCAGGGCGGCAGC
  GGCAGCTGCTGCTGCTGCTGTGGCTGCTGCTGCTGCTGCAGCAGCAGAAGAGTCTGAGAGCAGTGAGGAG
  GACTCAGACAGTGAGGACGAGGCCCCTGCTGGTCTGCCCAGCCAGGTAAAAGCCTCTGGAAAAGGTCCCC
  ATGTCAGAGCCGACTCGGTGTCTGCCAAGGGGATCTCTGGGAAAGGGCCCATCTTAGCAACCCCAGGGAA
  GACTGGGCCTGCAGCCACCCAGGCCAAGGCAGAAAGGCCAGAGAAGGACTCGGAAACCAGCAGTGAGGAC
  GATTCTGATAGTGAGGATGAAATGCCAGTCACTGTGAATACTCCTCAGGCAAGGACTTCTGGGAAGAGCC
  CTCGGGCCAGAGGTACCTCAGCCCCCGCCAAGGAGTCATCCCAGAAAGGGGCTCCTGCAGTCACCCCTGG
  AAAGGCAAGGCCTGTGGCAGCCCAGGCAGGGAAACCAGAAGCCAAGAGCAGTGAGGAGTCAGAGAGTGAC
  AGTGGGGAGACACCAGCTGCTGCGACTCTGACCACGAGTCCTGCCAAGGTGAAACCTTTGGGGAAGAGCT
  CCCAGGTCAGACCTGTTTCCACCGTCACCCCGGGGTCATCGGGAAAAGGTGCCAACCTGCCCTGCCCTGG
  GAAGGTGGGGTCAGCAGCTCTCAGGGTCCAAATGGTAAAGAAAGAAGATGTCTCGGAGAGCAGCAGTGCA
  GAGCTGGACAGTGACGGGCCTGGGAGCCCAGCCAAGGCAAAGGCCTCCCTTGCGCTCCCTCAGAAGGTGA
  GGCCTGTGGCCACCCAGGTCAAGACTGACAGGGGCAAAGGCCACTCAGGGAGCAGTGAGGAGTCATCTGA
  CAGCGAAGAGGAGGCAGCACCAGCAGCCTCTGCTGCTCAGGCTAAGCCAGCTCTGGAAAAGCAGATGAAA
  GCTTCCTCTAGGAAAGGCACGCCTGCATCCGCAACAGGAGCGAGCACCTCGTCCCATTGTAAGGCAGGAG
  CGGTGACCTCTTCAGCCAGCCTGTCATCCCCAGCTCTGGCCAAGGGCACCCAGAGGTCAGATGTGGACTC
  TTCCAGTGAGTCTGAGTCAGAAGGAGCTGCTCCCAGCACCCCCAGGGTACAGGGGAAGTCTGGGGGCAAG
  GGCCTCCAAGGGAAAGCTGCCTTGGGGCAAGGGGTGGCCCCAGTGCACACTCAGAAGACAGGGCCTTCGG
  TCAAAGCTATGGCTCAGGAAGACTCAGAGAGCCTCGAGGAGGACTCCAGCAGTGAGGAAGAGGATGAGAC
  CCCAGCACAGGCCACGCCCTTGGGGAGACTTCCTCAGGCCAAAGCCAACCCACCTCCCACTAAGACACCT
  CCAGCGTCTGCATCTGGAAAAGCTGTGGCTGCTCCAACCAAGGGAAAACCACCTGTTCCGAACAGCACCG
  TCTCTGCAAGGGGCCAGCGGTCTGTGCCAGCCGCGGGAAAAGCAGGGGCCCCAGCAACCCAAGCCCAGAA
  GGGTCCCGTGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTAAAGAGGAGTCTGACAGTGAAGAAGAG
  ACGCCAGCCCAGATAAAACCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGAGT
  CTCCTAAAAAAGGAGCCCATCCAGGAACCCCCGGCAAGACGGGATCTTCAGCTACCCAGGCCCAGCCAGG
  GAAGACAGAGGACTCAGACAGCAGTAGTGAAGAGTCTGACAGTGACACAGAGATGCCATCAGCCCAGGAT
  GCTATCTCCCAGCCTGCCAGAGGCAAAGCGTCAGGTCCAGCGTCCCCAGAGAAGAGCATAGAAGGGTCCT
  CAGAGAGCAGTGATGAGGATCTGCCCTCCGGCCAGGCGATTAAATCCCCTCCAGTTTCTGTCAACCGTAA
  CAGTAGTCCAGCTGTCCCAGCTCCTACCCCAGAAGGAGTCCAGGCTGTGAACACCACAAAGAAGGCCTCA
  GGCACCACTGCCCAGAGCTCCTCCTCTGAGAGTGAGGACGGGGACGAGGACTTGATTCCTGCCACACAAC
  CCTCCACCTATGCTCTCAGAACCAGTGTGACGACGCCCGCAGCCCTCTCACGAGCAGCTTCCCAACCCAG
  CAAGAGTGAGCAGTCTAGCCGGATGCCAAAAGGCAAGAAAGCAAAGGCGGCGGCGTCCGCTCAGACCAGC
  AGTGCCGTGGAAACACTCCCCATGATGCCTCCCCAGAGCGCACCCATCCAGCCCAAAGCCACCAACAAGC
  TCGGGAAATCCAAGCTCCCTGAGAAGCAGCAGCTTGCCCCAGGCTACCCCAAGGCCCCCAGGAGCTCGGA
  GGACAGCAGTGACACTTCTTCCGAGGACGAGGAGGATGCCAAGAGACCCCAGATGCCCAAGTCGGCCCAC
  AGGCTGGATCCAGACCCTTCCCAGAAGGAAACTGTGGTAGAGGAGACCCCTACAGAATCCAGCGAAGATG
  AGATGGTGGCCCCCTCACAGTCTCTCCTCTCAGGTTACATGACTCCCGGCTTGACTGTGGCTAATTCCCA
  GGCTTCAAAAGCTACTCCTAGGCCAGACTCCAACTCCTTAGCTTCTTCTGCCCCGGCCACCAAAGACAAC
  CCGGATGGCAAGCAGAAGTCAAAATCCCAACACGCAGCAGACACCGCACTCCCTAAAACCGGTAGGAAAG
  AGGCCTCCTCGGGCTCCACGCCTCAGAAGCCCAAGAAGCTCAAGAAGAGCACCTCAAGCTCGCCGGCCCC
  CACACAGACACTGCCGAACAGCATCACCCAGCGCCTCCTGGAGCAGGCCTGGCCCCTGAGCGAGGCGCAG
  GTGCAGGCCTCTGTGGTGAAGGTCCTGACAGAGCTTCTGGAGCAGGAACGGCTGAAGGCCACAGAGGCCA
  TCAAGGAGAGTGGGAAGAAGAGCCAGAAGCGGAAGTTATCAGGGGACCTAGAAGCTGGGGCCCCAAAGAA
  CAAGAAGAAGAAGGAGCAGCCAGTGCCCAGGGCAAGCGCTGTTTCCCCAGAAAAGGCTCCCATGACTTCC
  AAGGCAAAATCAAAGCTTGACAAAGGGAGTGCTGGTGGCAAGGGGAAGGGGTCTCCTGGCCCCCAAGGAG
  CCAAGGAGAAGCCCGACGGCGAGTTGCTGGGGATAAAGCTTGAGAGTGGCGAGCAGAGCGACCCGAAGAG
  CAAGTCGAAAAAGAAGAAATCCCTCAAGAAAAAAAAAGACAAGGAGAAAAAGGAAAAGAAGAAAGGAAAA
  AAGTCCCTGGCCAAAGACTCTGCCTCGCCGATCCAGAAGAAGAAAAAGAAGAAGAAGAAGTCAGCCGAGC
  CTGCCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198984
• Insert Size: 4071 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198984.1, NP_001185913.1
• RefSeq Size: 4679 bp
• RefSeq ORF: 4071 bp
• Locus ID: 21453
• Cytogenetics: 18 34.41 cM
• Gene Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.