Cobl (NM_001282993) Mouse Untagged Clone

CAT#: MC229553

Cobl (untagged) - Mouse cordon-bleu WH2 repeat (Cobl), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cobl
• Synonyms: 4732460E13; C530045F18Rik; mKIAA0633
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229553 representing NM_001282993
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGCCGCGTGCACTGGCGGCCAAGCCCCCCACAGGAAGGAAGATGAAGGCCCGTGCTCCGCCAC
  CTCCTGGAAAGCCTGCTGCCCAGAATGTCCACAGCGAACAGAAACTGCCCCATGATGCCACCCTGGGATC
  CCAGCAAAGCCTGGTCTACATGAAGGAGGCACTGCAGAACAGCACCTTGGACATCACCGTGGTTCTGCCC
  AGTGGACTGGAGAAGCAGAGTGTGGTTAGTGGGAGCCACGCAATGATGGACCTACTGGTTGAACTCTGCC
  TTCAGAACCACCTGAATCCTTCTCACCACGTCCTAGAGATCTGGTCTTCTGAGACCCAACAGCCTTTGAG
  TTTTAAGCCAAATACGTTGATTGGGTCCTTGAATGTGCATACTGTACTTCTGAAAGAAAAAGTCCCTGAA
  GAAAGAGTTAAACCTGGCCTAACCAAGGCTCCTGAGAAATCTGTACGACTGGTAGTGAACTACCTGAGAA
  CGCAGAAGGCTGTCGTGCGGGTGAGCCCTGAGGTGCCACTACAGAATATTCTGCCAGTCATCTGTGCGAA
  GTGTGAGGTCAACCCAGAACACGTGATTCTGCTCAGAGACAATGTTGCCGGGGAGGAGCTGGAGCTGTCC
  AAGTCCCTGAATGAACTAGGGATCAAGGAGCTGTATGCGTGGGACAACAGAAGAGAAATGTTTAGAAAAT
  CATCACTTGGCAATGATGAGACAGACAAAGAGAAGAAAAAGTTTCTGGGATTTTTCAAAGCTAATAAAAG
  GAGCAACAGTAAGGGCTGCGTAACAACACCCAACTCACCATCCTTGCATTCTCGGTCCCTAACACTGGGT
  CCCTCCCTCTCCCTGGGCAACATCTCTGGAGTGTCTATGAAGTCAGACATGAAGAAGCGCAGAGCCCCTC
  CTCCTCCAAGTCCCAAGCTGCTGGGTCAAGACAAGGTATCAGAAAAGGCCTCCCTCAGCTCACAGGCTGA
  TCTCCAGAAGAAGAAGAGGCGGGCGCCAGCTCCTCCTCCACCACAGCAGCCACCACCAAGTCCTGTGGTC
  CCCAACCGCAAGGAAGATAAGGAAGAGAACAGGAAGAGCACAGTGGGTGTTGGACGTCAGGTGCCACAAA
  AGCCTCCCAGAGGCACAGCTCGGGGCCCACCCCAGTTAGTGCTCCCCCCGCCCCCACCCTACCCTCCTCC
  TGACACAGATGTGACAGAGCCTGTCACCTTTCCTGGGGAAGGCGCTGGTTCCGAGACCTCAGAGCTGAGA
  CCCAAACTGAGTCTGCCTCTGGGGCCTGGCAGCCACTGCAGCATGGGTGGAGTCTCTCAGGTGCCAGCGG
  AGAGTGAGGAGACAGCATCTGAGGACACAACCGAGGACTCGGGGGTCATGAGCTCTCCCTCAGATGCCAT
  CTCCCTGGACTCACAACAAGACAGCATGAGATCCAAGGACAAATGGTCCACAGACCAGGAAGACGGCAGT
  GACCAGGACCTGGCTGGAACTCCAGAACTGGGTCCCCAAAAGAGCCCATCATGGGGGAAAAGTGGTTCAG
  GAAGCTCAATTCTAAGGACTGAGAAAGCCACCATGCCTACCAATGATGATGAAGACCTATTCATCACTGG
  CCACTTACATCAGACCCTGGCAGAACTTGATGAAGACCTGGAAGGAATGGAAGAAAATTATGAAACAGAT
  ACTAGCTCTCTAACCAACTCTGTCAATGGTGTATCCAACCACAGTCTGCAAGAAGCCATAATCCCTGATA
  GTGGCGTGGATGACATCCCAGTCACTTTTATCGGGGAAGTTTCTGATGAGCCTTTTGACTCAGGGCTGTT
  CTCCAGTAGATGTAACAATGCCACTACTTTTAACACGGGGAGCATTGCTAGCCAAAGATCTCATCTGTCC
  CCATCTCAGACTGAGCATAGCCAGCCATTCGTAAGGACAAGTAGAAAAGAGCCTGATCCCTCCCCTCCTT
  CCCAAGATAACAGGAAGAGAAATCAGCCAACCTTAGCCAACACATCTGAAAATGAGAATCCAGTTGAAAC
  AGACCCCACAGTTACATCACTTGTTTCAAAGCTTTTAATAGATGACCCAAAAGCAAAGGATAAAGGCAAA
  GTGCATGGTTCTAGTCACAGTGAGAAGACACAGGCAGGTCATGGAATAAACTCACTGCGAGTGAATCCAA
  GAGATGGTAAGGATGAGAGCTCCAATTCAGCACCCCCACCATGGTCTCATCATGGCCAGGCCTTAGGGGG
  AAGCTATGGACTCAAGTATGGCCTCACGACATATAAAATTGTTCCTCCCAAATCAGAGATGAGGTGTTAT
  GACAGGGATGTGTCCCTCTCCACTGGTGCCATTAAGATTGATGAACTAGGGAATCTGGTGAGTCCTCACA
  TGAACGGAAGCAGGACCATATCCCCACCATCAGCTGTTGTCGAAACAGACACTCCACCTATTGGAAAAGT
  CAAAGAGTTCTGGAGGCGCAACTCAATGGAAAAGTACCTTAATGGGCCGGCTGAGTGCACTATCAAAAGG
  GCCCCCTCCACCACTATCACTGCCACACCAGAAAAACCACAGCAAGATAATGGGATGAAGGCAGCCTTCA
  CAGTGACCACTCCCCAGCAGCAGCCTGCCTCACAGGAATATGGGGCACACCTGGAGGAAGAGAGAAGCCG
  GCCACAGTCAGCAGTCTCTTGTTCTGTGAAAGTGCCAGCATCTAATCCCACAGACATCACATTCCTCAAG
  CCTCAAAGAAGAACATCCAGCCAGTATGTGGCCTCTGCTATTGCCAAGAAGATGGGGCCTCCAAAAGTCC
  ATGCTGATGTGGTGAGACCACATAAGGCAACCACTGAACAGTGTCATGAAGAAGCAAAGCTGGCTAGATC
  TCCTCCCACCAGGAAAGATGATGCAGCTCCCAACCTACACTCAGAGGCAAGGCAGCATGAACATGGGACA
  AACCAGAGCTCTGTCTGCCTTCCCAGCAACCCTGGTGTGCAATTGCCAGCAGGAGGCCATCCCAAAGTAG
  AGGTCAACAGCACATATGGGAAGTCATCGACTCAAGACTATCCTGCTGCTGTCCACAGAAACTCCTATTT
  CCTCCCAGGCAGATCTTCCCATAGGGATCGTGTTTCTGTGGGACAGAGCTGTGGTTTCAATGAAAAGCAA
  ACTACAAGTAACCAAAAGGCAAATTCAACATCTAACTTCTCGCAGGCACTGGACAAGGCGCATCCACCCC
  CTCTGCTCTTGGCAGAGGCCCGTGACTCTGGGAGGATACTGATGAATGGCTCTGCACGAACCCCAGGAAA
  TTGTGAGCCCCCCCACTCTCCAAAAGAATCCACCCTGACTAGCTATATTATCTTACAGACAGAGGAAAAG
  CCCAGTTCGTTATCTACAGATGGTCAAGACGCAGATGATACTCTACCGTCCAGCATTTTTGGGCCAAAGA
  AAAAGTTCAAGCCTGTCATTCAAAGGCCACTCCCAAAAGATGTATCCCTGCACAGTGCCCTGATGGAAGC
  TATCCACTCATCAGGAGGGAGAGAAAAGCTCCGGAAGACTGCAGAACAGACGTCAGAAGGAAGGCCAAAG
  AAACCATCCTACGTGGAGGCAGAGAGTGAGCGATCTGCCCTTCTGGCAGCCATTCGGGGTCACAGTGGGA
  CTCTCAGCCTCAGGAAGGTGTCATCCCTTGCCTCTGAGGAGCTCCAGAGCTTCCGCAATGCTGCACTCGG
  GGCTCCAGGTTTGGATAAACCTCAGCAAGAAGACCTTGGCCTTCCACCCCCACCTGCCCTGCCACCACCA
  CCCGCTCCAGCCCCCCAGGCTCCTTCAGCCTCCGTAACAGTTTCTAGGTTCAGCACCGGCACCCCCAGCA
  ACTCAGTGAATGCTAGACAAGCCTTGATGGATGCCATCCGCTCGGGCACAGGAGCTGCAAGACTAAGAAA
  GGTTCCCTTGCTCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282993
• Insert Size: 3939 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282993.1, NP_001269922.1
• RefSeq Size: 5558 bp
• RefSeq ORF: 3939 bp
• Locus ID: 12808
• Cytogenetics: 11 7.3 cM
• Gene Summary: Plays an important role in the reorganization of the actin cytoskeleton. Binds to and sequesters actin monomers (G actin). Nucleates actin polymerization by assembling three actin monomers in cross-filament orientation and thereby promotes growth of actin filaments at the barbed end. Can also mediate actin depolymerization at barbed ends and severing of actin filaments. Promotes formation of cell ruffles. Regulates neuron morphogenesis and increases branching of axons and dendrites. Regulates dendrite branching in Purkinje cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.