Clasp2 (NM_001286600) Mouse Untagged Clone

CAT#: MC229549

Clasp2 (untagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clasp2
• Synonyms: 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229549 representing NM_001286600
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGCTGATTTGCAAACGGATCTGTGATTATAAAAGCTTTGATGATGAGGAATCAGTGGATGGAA
  ATAGGCCGTCGTCAGCTGCTTCAGCCTTCAAGGTTCCTGCACCTAAAACACCTGGGAATCCTGTCAGCAG
  TGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGAGGTCCTTCTAAAGAAGGAGGGGCTGGAGCA
  GTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTCCTTCTGTTCAGATCTATTCTAGTCGAGAAC
  TTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGATGACAAACATGACTGGGACCAGCGTGCCAA
  TGCGCTTAAGAAAATCAGATCACTGCTTGTTGCTGGAGCCGCACAGTATGATTGCTTTTTCCAGCACCTA
  CGTTTGTTGGATGGAGCGCTTAAGCTGTCAGCTAAGGACCTCAGATCCCAGGTGGTCAGGGAAGCTTGCA
  TCACTGTTGCTCACCTTTCAACAGTCTTGGGAAACAAATTTGATCACGGCGCTGAAGCCATCGTCCCTAC
  GCTTTTTAATCTTGTCCCCAATAGTGCCAAAGTCATGGCGACCTCTGGATGTGCAGCAATCAGATTTATT
  ATTCGGCATACCCATGTACCTAGACTTATTCCTTTAATAACAAGCAACTGCACATCAAAGTCAGTTCCTG
  TAAGGAGACGTTCATTTGAATTTTTAGATTTGCTGCTGCAAGAATGGCAGACTCATTCACTGGAAAGACA
  TGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGCATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCA
  AGGAAGACATACATGGGCCTTAGGAACCACTTTCCTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGC
  CATCATATCAGAAAAGTCTTCAAACTTACTTAAAGAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGA
  CAGGTCCTCATCCAGCTCACAAGAAAGTCTCAATCGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCT
  TCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCAGCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTT
  CTCGAAGTGACATTGATGTGAATGCGGCAGCTGGTGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCG
  AAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCAGGCTCCTATGCATCACTAGAGGATACTTCTGACAAG
  ATGGATGGAACAGCATCTGATGATGGTCGGGTGAGAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAA
  ATGCCAAGACCGACTCTAGAGGGAGAAGCCGGACAAAAATGGTGTCTCAGTCCCAGCGTTCATCATCACC
  TGACAAAAATGAAGGATCACAATCTGCTAATACCATTGGTGCTGGCAGCCGATCTGGGTCTCCAGGAAGA
  GTTCTAACCACAACAGCCCTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTGGTCAATTCAGCTTCAG
  CACAGAAGAGAAGCAAGATCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCCCATCTCGGCTCTCAGT
  GGCCCGGAGCAGCCGTATTCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGAAGCCAGCAGAGAGAGC
  AGCAGGGACACGAGTCCGGTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGGATCAGCCAGTCCAGCC
  GGTTGTCGTCCTCTGTCAGTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGGAGGAGGCAGTAGCTGA
  TGCCCTGCTCTTAGGAGACATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTATGAATCATATGGAATG
  CACTCAGATGATGATGCCAACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCCTATAGCTCTCGAAATG
  GTAGTATTCCTACCTACATGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACAGATGTGCTAGCTCCAA
  TTGGTCAGAGAGGAAAGAAGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCAGAGAACGCTAAGTCGA
  GTTGAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCAGATCCTCATGGCAAGGTGTTCAGCA
  TGTTCTTGGAGACTCTAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTTCT
  GCTGACACAGCTGCTGAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGCAAAAGTTCAGAAAGCC
  CTTGATATTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATGAGATTTACAGTTGACC
  AGACCCAAACGCCAAGCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAACTCTGGCAAAGCAGAT
  GGACCCAAGAGATTTTACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGTCATCACTTGGACGACA
  GAGCCCAAAAGCTCTGATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTATTTGAACTCAATACCC
  CAGAGTTTACAATGTTACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTACTAAACTTCTTCACAA
  TCACCTCCGGAACACTGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGACGAGACCAACACCTCGG
  TCACCAGCCAACTGGTCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAATACGTTATCTCCAAGTG
  CATTTGATTACGATACAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTAGAGGCGTCACTGAGGC
  AATCCAGAATTTCAGCTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAGGGACCCTAAAAAGGAG
  GATGGTGACACAATATGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGTGATGCTGCTGACGGCA
  GCCAGCCAGCTCTGGATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACTCCTCTCCACGCTCCCG
  TGACTATAACCCATATAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGCCCTCAAGGAAGCCATG
  TTTGATGATGACGCCGACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGACCTAGTTGCAGAGTTGT
  TGAAGGAGCTGTCTAATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGTATGAACTCATGAAGCT
  AACCCAGGAAGAATCTTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATTGTTATTGCTTGAGACC
  CTTGGGGATAAAGAGCCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAAATCTTAAGGCATCAAC
  CAGCAAGATTCAAAAACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCACATAAAGATCCTCACAA
  AGAGGTGGTGAGATCTGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAGTCCAGAGCAGTGCATC
  AAAGTGCTTTGTCCCATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCAATCAAAATGCAAACAA
  AAGTGATAGAGAGAGTATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCATGCCGGGTCTAATACA
  GGGTTATGATAATTCAGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGTGGCTGTCCATGCAGTG
  ATTGGTGATGAACTAAAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAGCTGCTGAACCTTTACA
  TCAAGCGTGCACAGACGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTGGACAGAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286600
• Insert Size: 3915 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286600.1, NP_001273529.1
• RefSeq Size: 5678 bp
• RefSeq ORF: 3915 bp
• Locus ID: 76499
• UniProt ID: Q8BRT1
• Cytogenetics: 9 F3
• Gene Summary: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' and 3' coding region, and initiates translation at an alternate start codon, compared to variant 3. It encodes isoform e, which is shorter and has a distinct N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.