Rreb1 (NM_026830) Mouse Untagged Clone

CAT#: MC229544

Rreb1 (untagged) - Mouse ras responsive element binding protein 1 (Rreb1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rreb1
• Synonyms: 1110037N09Rik; AA414966; B930013M22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229544 representing NM_026830
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCGAATTCGCCCATTGGTTTAGAAGGCTCAGACCTGTCTTCCATCAACACCATGATGTCAGCAG
  TAATGAGCGTAGCGAGTGTCACAGAGAATGGTGGGAGCCCCCAGGGCATCAAGTCCCCCATGAAACCTCC
  AGGACCAAATCGGATTGGCAGAAGGAACCAGGAAACGAAAGAGGAGAAGTCTTCCTACAACTGTCCCCTA
  TGTGAAAAGATTTGCACCACCCAGCACCAGCTGACCATGCACATCCGTCAGCACAACACAGACACGGGAG
  GAGCTGACCACGCATGCAGTATCTGTGGGAAGTCGCTGAGCTCGGCCAGCTCCCTGGATCGTCACATGCT
  GGTGCACTCTGGCGAGAGGCCTTACAAGTGTACAGTGTGTGGCCAGTCTTTCACCACCAATGGGAACATG
  CACAGACATATGAAGATTCATGAGAAGGATACCAACAGTACTACAGCTGCAGCCCCTCCATCCCCTCTGA
  AGCGCAGGCGGTTGTCCTCCAAAAGGAAGCTGAGTCACGATGCCGAGTCAGAAGACCCAGGACCAGCTAA
  AAAGATGGTAGAAGACGGGCAGTCAGGCGATTTGGACAAGATGAGCGATGAAATCTTTCACTGCCCAGTG
  TGTTTCAAGGAGTTTGTTTGCAAGTATGAACTGGAGACCCACATGGAGACCCACTCAGATAACCCACTAA
  GATGTGACATTTGCTGCGTCACCTTCCGCACACATCGAGGACTGCTGCGCCACAATGCACTTGTCCACAA
  GCAGCTTCCCAGAGATGCCATGGGAAGACCTTTTATCCAGAACAACCCTTCGATTCCTGCTGGCTTCCAT
  GATTTAGGGTTTACTGACTTCTCCTGTAGGAAGTTTCCTCGAATCTCTCAGGCCTGGTGTGAGACAAACC
  TACGGCGGTGCATCAGCGAGCAGCACCGGTTTGTGTGTGACACCTGCGACAAGGCGTTCCCCATGCTGTC
  GTCACTCATCCTGCACAGGCAGAGCCACATCCCTGCCGATCAGGGACGGGAGAAGCTCCAGACCAAGACC
  CTGGCTGCCGAGTCCTTGGAACAGAAGGCCTTCCTGGCCTTGCTGGGCCTGCAGCACACCAAAGACGTCA
  AGCCTGCCCCGGCGGAGGAGCTCCTGCCGGACGACAACCAAGCAATACAGCTCCAGACACTTAAGTACCA
  GCTACCTCAGGAGCCTGGCTGCCCCACCGTGCTGAGTGTGTCTCCTCTTGACGCTGCTTCTTTAGGTGGC
  TCTCTGACAGTCCTCCCAGCTACCAAGGAGAATATGAAGCATCTGTCCCTGCAGCCCTTCCAGAAGGGCT
  TCATCATCCAGCCAGACAGTAGCATTGTGGTTAAGCCTATTTCAGGAGAGTCGGCCATTGAGCTGGCAGA
  TATCCAACAGATTCTAAAGATGGCAGCTTCCGCTCCTCCGCAGATCAGTCTTCCGCCACTTTCCAAGGCC
  CCTGCCACCCCGCTGCAGGCGATTTTCAAGCACATGCCTCCTTTGAAGCCAAAGCCCTTGGTCACACCCC
  GGACAGTGGTAGCCGCCTCCACGCCCCCACCTCTCATCAACGCCCAGCAGGCATCTCCCGGTTGTATCAG
  CCCCAGCCTTCCTCCACAGTCCCTGAAGTTCCTCAAGGGGTCGGTGGAGGCAGTGTCCAATGTTCATCTG
  CTTCAGTCCAAGTCTGGGATCCAGCCAAGCACCACCACACAGCTCTTCCTGCAGCAAGCTGGAGTGGAGT
  TGCCGGGCCAGCCCGAGATGAAGACACAGCTGGAACAAGAGAGTATCATTGAGGCCCTGCTGCCCCTCAA
  CATGGAGGCGAAGATCAAGCAGGAGATAACAGAAGGTGACCTCAAGGCCATCATGACAGGCCCCAGTGGC
  AAGAAGACCCCCGCCATGCGCAAGGTGCTCTACCCCTGCCGCTTTTGCAACCAGGTGTTTGCTTTCTCTG
  GAGTCCTGCGAGCCCACGTTCGCTCCCACCTGGGCATCTCACCCTACCAATGCAACATCTGTGACTATAT
  TGCTGCAGACAAAGCCGCCCTGATCCGTCACATCCGCACACACAGCGGGGAACGGCCTTACATCTGCAAG
  ATCTGCCACTACCCATTCACGGTCAAAGCCAACTGTGAGCGGCACCTGCGCAAGAAGCACCTCAAGGCCA
  CCAGGAAGGACATTGAGAAGAACATAGAGTACGTGAGCAGCCCCACCGCAGAGCTGGTGGACGCCTTCTG
  CGCCCCAGAGACCGTGTGCAGACTGTGCGGGGAGGATCTGAAGCACTACCGAGCTCTACGCATCCACATG
  CGCACACACTGCAGCCGGGGCCTGGGCGGCTGCCACAAAGGCCGGAAACCCTTCGAGTGCAAGGAATGCA
  ACGCTCCCTTCGTGGCCAAGCGCAACTGCATCCATCACATCCTCAAGCAGCACCTGCACGTGCCCGAGAA
  GGACATCGAGAGCTACGTGCTTGCCACCAACAGTGGCCTCGGCCCCGCGGACACGCCCACGGATGCCGCT
  TCCAGAGGAGAAGAGGGCAGCTGTGTCACTTTCGCGGAGTGCAAGCCCCTCGCCACTTTCCTGGAGCCCC
  AAAATGGCTTTCTTCACTCGAGCCCCACCCAGCCCCTGCCTTCCCACATCTCTGTCAAGCTGGAGCCAGC
  CAGCAGCTTTGCCATGGACTTCAACGAACCCCTTGACTTTTCGCAGAAAGGCCTGGCACTGGTCCAAGTG
  AAGCAGGAAAATGTTTCCTCCTTGCTGACGTCTTCCTCCTCCTCTGCCCTCTATGACTGCTCCATGGAGC
  CCATTGACCTGTCCATCCCCAAGAGCGTCAAGAAAGGAGACAAGGACACAGTTGTTCCCAGTGACGCCAA
  GAAACCGGAACCGGAAGCTGGGCAAGCCGAGCCGCTCTCACCCCGCCCACCACCCTGCCCTACCTTGTCA
  GTGACTGTGGAGCCCAAGGGGAGCCTGGAAACCCCCACAGGCACGGTGGTGGCCGTCACCACAGCTGCCA
  AGCTAGAGCCCCACACTCAGCCCCTCCAGGGCTCCGTGCAGCTGGCTGTCCCCATCTACTCCCCCGCGCT
  CGTCAGCAATACTCCCCTCTTGGGCAATTCTGCTGCCCTCTTGAACAACCCAGCCTTGCTTCGGCCCTTA
  CGGCCAAAGCCCCCCCTCCTCTTGCCAAAGCCCTCGATGACAGAGGAGCTGCCGCCACTGGCCTCCATCG
  CCCAGATCATTTCTTCCGTGTCCTCGGCCCCTACTCTGCTGAAAACGAAGGTAGCTGACCCTGGACCATC
  GATCACCAGCAGTAACACTGTGGCCACAGACAGCCCAGGAAGCTCCATCCCCAAAGCTGCCGCCACCCCC
  ACTGACACCACAAGCTCTAAAGAATCCAGTGAGCCACCCCCTGCAGCCAGCAGCCCTGAGGAAGCCTTGC
  CTACTGAACAAGGGCCGGCTGCCACTTCCTCGTCGAGGAAGAGGGGCAGGAAAAGGGGACTGAGAAACCG
  GCCCCTCCCCAACAGCAGTGCTGTGGACCTGGACTCCAGTGGGGAGTTTGCTAGCATCGAGAAGATGCTG
  GCCACCACAGATACCAACAAGTTCAGTCCCTTTCTGCAGACTGCAGAGGATGACACTCAGGAAGAGGTGG
  CTGGAGCCCCTGCCGACCAGCATGGGCCCGCTGATGAGGAGCAAGGTAGCCCCGCAGAAGACAGGCTGCT
  GAGAGCAAAGCGGAACTCCTATGCCAACTGCCTGCAAAAGATCAACTGTCCCCACTGTCCCCGGGTCTTC
  CCTTGGGCCAGCTCCCTGCAGCGGCACATGCTTACACACACTGGTAAGAAGGCCCTCACGGCTCACCAGG
  CGGTGAGTCTTGAAAGGAAAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026830
• Insert Size: 3876 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026830.2, NP_081106.1
• RefSeq Size: 7437 bp
• RefSeq ORF: 3876 bp
• Locus ID: 68750
• UniProt ID: Q3UH06
• Cytogenetics: 13 A3.3
• Gene Summary: Transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1 (By similarity). Binds specifically to the allelic variant of the CDKN2A promoter present in Balb/c mice, which leads to a down-regulation of CDKN2A expression in this strain, and, as a consequence, to an elevated susceptibility to pristane-induced tumors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 2. Variants 2-4 encode the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.