Cadps2 (NM_001252110) Mouse Untagged Clone

CAT#: MC229524

Cadps2 (untagged) - Mouse Ca2+-dependent activator protein for secretion 2 (Cadps2), transcript variant 7

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cadps2
• Synonyms: A230044C21Rik; Caps-2; Caps2; Cpd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229524 representing NM_001252110
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGACCCGTCTTCCAGCGAGGAGGAGTCGGACGAGGGGCTGGAGGAGGAGAGCCGCGAGGTGTTGG
  TGGCGCCGGGCGTCTCCCAGCGAGCGCCGCCGGCCGCGGCTCGGGAAGGGCGGCGGGACGCGCCGGGACG
  CTCCGGTGGTGGCAGCGGCGGCGGAGCGGCCAGACCCGTGAGCCCGAGCCCCTCGGTACTCAGCGAGGGG
  CGAAATGAGCCCGAGCTGCAGCTGGACGAGGAGCAGGAGCGGCGCATCCGCCTGCAGCTCTACGTCTTCG
  TGGTGAGGTGCATCGCGTACCCATTCAACGCCAAGCAGCCCACCGACATGGCCCGGAGGCAGCAGAAGCT
  TAACAAACAACAATTGCAGTTACTGAAAGAACGGTTCCAGGCCTTTCTCAATGGAGAAACTCAGATAGTA
  GCAGACGAAGCCTTCTGCAATGCCGTCCGGAGTTACTATGAGGTGTTTCTAAAGAGTGACCGTGTGGCCA
  GAATGGTTCAAAGTGGAGGATGCTCTGCTAATGATTTCAGAGAGGTGTTTAAGAAGAACATAGAAAAACG
  TGTCCGTAGCCTGCCAGAGATAGATGGCCTGAGCAAAGAGACAGTGCTGAGCTCATGGATAGCCAAATAT
  GACGCCATTTACAGGGGAGAAGAAGATTTGTGCAAACAGCCGAACAGGATGACCCTCAGTGCCGTGTCTG
  AGCTTATTCTGAGTAAGGAGCAGCTCTATGAAATGTTCCAGCAGATTCTGGGCATTAAGAAGCTGGAACA
  CCAGCTACTTTATAACGCATGCCAGCTGGATAACGCTGATGAACAAGCAGCCCAGATCAGAAGGGAACTT
  GATGGCCGCCTGCAGTTGGCAGAGAAGATGGCAAAGGAGAGAAGATTCCCCAGATTCATCTCGAAAGAAA
  TGGAGAGTATGTACATTGAAGAGCTGCGAGCCTCCGTGAACCTGCTAATGGCCAATTTAGAAAGTCTTCC
  AGTTTCAAAAGGTGGCCCGGAATTTAAATTACAAAAATTAAAGCGTTCGCAGAACTCTGCGTTTCTGGAC
  CTGGGAGATGAGAACGAGATCCAGCTGTCCAAGTCGGATGTGGTGTTGTCGTTCACGTTAGAGATTGTCA
  TCATGGAAGTGCAAGGACTGAAATCTGTGGCTCCCAATCGAATCGTTTACTGCACAATGGAGGTGGAAGG
  AGGAGAAAAACTCCAGACAGACCAGGCTGAAGCATCAAGGCCACAATGGGGGACCCAAGGAGATTTCAAC
  ACTACCCACCCTCGGCCTGTCGTCAAAGTGAAGCTCTTCACAGAAAGCACGGGGGTCCTGGCCCTGGAAG
  ACAAGGAACTGGGCAGGGTGGTGTTATACCCAACTTCTAATAGCTCCAAGTCAGCAGAGTTACACCGAAT
  GACAGTACCCAAGAACAGTCAGGACTCGGACCTAAAGATCAAATTGGCAGTGCGCATGGATAAACCAGCA
  CACATGAAGCATAGTGGGTACCTGTATGCCCTTGGACAGAAGGTTTGGAAAAGATGGAAAAAGCGTTACT
  TTGTTCTCGTTCAGGTTAGCCAGTACACCTTTGCTATGTGCAGCTATAGAGAAAAAAAGTCGGAACCACA
  GGAATTAATGCAACTGGAAGGATACACAGTGGATTACACAGACCCTCACCCAGGCCTTCAGGGTGGTCAG
  GTGTTCTTCAACGCTGTTAAAGAGGGAGATACTGTGATCTTTGCCAGTGATGATGAACAGGACAGAATAT
  TATGGGTACAAGCCATGTACAGGGCTACAGGCCAGTCTTACAAACCAGTTCCTGCAGTCCAAAGCCAGAA
  GCTGAATCCTAAAGGCGGAGCTCTCCATGCAGATGCTCAGCTTTATGCAGACCGTTTTCAGAAACACGGG
  ATGGATGAGTTTATTTCTGCGAGTCCTTGCAAGCTTGACCATGCCTTCCTCTTCAGAATTCTCCAGAGAC
  AGACTTTGGATCACAGACTGAATGATTCGTATTCTTGTTTGGGGTGGTTTAGCCCTGGCCAAGTCTTTGT
  GTTAGATGAGTACTGTGCCCGCTACGGAGTGAGAGGCTGTCACAGGCATCTCTGCTACCTTACAGAACTG
  ATGGAACATTCAGAAAACGGTGCTGTCATTGACCCCACCCTGCTCCATTACAGCTTTGCATTCTGTGCCT
  CTCACGTGCACGGCAACAGGCCTGATGGGATTGGAACGGTTTCAGTGGAAGAGAAAGAAAGATTTGAGGA
  GATAAAAGACCGACTTTCTTCGCTTTTAGAAAACCAGATCAGCCACTTCAGATACTGCTTTCCCTTCGGA
  CGACCTGAGGGTGCCCTAAAAGCTACGCTCTCCTTACTTGAAAGGGTTTTAATGAAAGACATTGCCACTC
  CTATCCCTGCGGAGGAGGTGAAGAAAGTGGTCAGAAAATGTCTGGAGAAAGCTGCCTTGATCAATTACAC
  TAGGCTCACAGAATATGCCAAAATAGAAGAGACCATGAACCAGGCAACTCCTGCCAGGAAGCTGGAAGAG
  GTTCTTCATCTTGCAGAGCTCTGCATAGAAGTCCTACAGCAAAATGAGGAGCATCATGCTGAGGCATTTG
  CCTGGTGGCCTGACTTGTTGGCCGAGCATGCAGAGAAGTTTTGGGCTTTATTCACAGTAGACATGGATAC
  TGCGCTGGAGGCCCAACCTCAAGACTCCTGGGATAGCTTTCCCCTTTTCCAGCTGCTTAATAATTTCCTC
  AGAAATGACACACTTTTGTGTAATGGAAAATTCCACAAGCACTTGCAAGAAATCTTTGTGCCCTTGGTTG
  TCCGCTACGTTGACCTGATGGAGTCTGCCATCGCCCAGTCCATTCACAGAGGTTTTGAGCAGGAGACATG
  GCAGCCTGTCAACAATGGCTCAACAACTTCCGAGGATCTGTTCTGGAAGCTCGATGCGCTGCAAATGTTC
  GTCTTTGATCTCCATTGGCCAGAACAAGAGTTTGCCCACCACTTAGAGCAAAGACTTAAACTAATGGCCA
  GTGATATGATAGAGGCGTGTGTCAAAAGAACAAGAACTGCGTTCGAACTCAAGCTACAAAAGGCAAACAA
  AACAACTGACTTGCGCATCCCAGCTTCCGTGTGCACAATGTTTAATGTATTAGTTGATGCTAAAAAGCAA
  AGCACCAAGCTGTGTGCCCTGGATGGAGGACAAGAGTTTAGGAATCAGTGGCAACAGTACCATTCAAAAA
  TAGATGATTTGATTGACAACACCGTGAAAGAAATCATTGCACTGCTGGTTTCAAAGTTTGTTTCAGTGTT
  GGAAGGGGTGCTTTCGAAGTTGTCGAGGTATGACGAAGGCACTTTCTTCTCATCCATCCTGTCCTTCACT
  GTGAAAGCAGCTGCAAAATATGTGGATGTCCCTAAACCAGGAATGGATCTGGCAGACACCTACATTATGT
  TTGTCCGGCAAAACCAGGATATTCTTCGAGAAAAAGTCAATGAAGAGATGTACATAGAAAAGTTGTTTGA
  TCAATGGTACAGCAATTCCATGAAAGTCATTTGTGTGTGGCTGGCTGACAGACTAGACCTCCAGCTTCAT
  ATTTACCAACTGAAGACGCTCATCAAGATTGTGAAGAAAACCTATAGGGATTTCCGATTGCAGGGTGTGT
  TGGAGGGGACGCTGAACAGTAAGACATATGATACTCTGCACAGACGTCTAACTGTAGAGGAGGCCACAGC
  CTCTGTCTCAGAAGGCGGAGGACTTCAGGGCATTACCATGAAGGACAGTGATGAGGAGGAAGAAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252110
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252110.1, NP_001239039.1
• RefSeq Size: 4834 bp
• RefSeq ORF: 3780 bp
• Locus ID: 320405
• Cytogenetics: 6 A3.1
• Gene Summary: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) lacks three in-frame exons in the central coding region, but includes an additional in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (7) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.