Magi1 (NM_001286786) Mouse Untagged Clone

CAT#: MC229501

Magi1 (untagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 7

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Magi1
• Synonyms: AIP3; Baiap1; BAP1; Gukmi1; Magi-1; MAGI1c; mKIAA4129; TNRC19; WWP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229501 representing NM_001286786
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAAGTGATCCAGAAGAAGAACCACTGGACTGGCCGCGTTCACGAGTGCACCGTGAAGCGGGGAC
  CCCAGGGCGAGCTGGGGGTGACGGTCCTGGGGGGCGCGGAGCATGGGGAGTTTCCGTACGTGGGGGCGGT
  GGCGGCGGCCGAGGCGGCGGGGCTTCCCGGCGGTGGCGAGGGGCCGAAGCTGGCCGAAGGTGAGCTGCTG
  CTGGAGGTGCAGGGGGTCCGGGTGTCCGGCTTGCCCCGCTATGACGTGCTGGGAGTCATCGACAGCTGCA
  AGGAGGCCGTCACCTTCAAAGCCGTCAGACAAGGAGGAAGGCTCAACAAGGACCTACGACATTTCCTCAA
  CCAACGGTTCCAGAAGGGGTCTCCAGATCATGAGCTCCAGCAGACCATAAGGGACAACCTCTACCGCCAT
  GCTGTGCCTTGCACAACCCGGTCTCCCAGAGAAGGAGAAGTGCCTGGTGTGGATTACAGCTTTCTGACTG
  TGAAGGAGTTCTTGGACCTCGAGCAGAGCGGGACCCTGTTGGAAGTCGGCACCTATGAAGGAAACTATTA
  TGGGACACCCAAACCTCCTAGCCAGCCAGTCAGTGGGAAAGTGATCACGACGGATGCCTTGCACAGCCTG
  CAGTCTGGCTCCAAGCAGTCGACCCCTAAGCGAACAAAGTCCTACAATGATATGCAAAATGCTGGCATAG
  TCCACCCGGAGAATGAGGAGGAGGAGGATGTCCCTGAAATGAACAGTAGCTTTACAGCCGACTCTGGAGA
  CCAGGACGAGCACACTCTCCAAGAAGCAACGCTCCCGCCTGTGAATAGTAGCATCCTCGCTGCTCCCATC
  ACGGACCCTTCTCAGAAGTTCCCTCAGTACCTACCTCTTTCTGCAGAGGATAATTTAGGTCCTCTACCTG
  AAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTCATAGACCACAACACGAAAACAACATC
  ATGGTTAGACCCTCGGTGCCTGAACAAACAGCAGAAGCCTCTGGAAGAATGTGAAGATGATGAGTTGCCT
  GCTGGCTGGGAAAAGATTGAAGACCCTGTCTACGGTGTCTACTATGTAGACCACATCAACAGGAAGACGC
  AATATGAAAACCCAGTCCTAGAAGCCAAACGGAAGAAACAGCTTGAACAGCAGCAGCAACAGCAGCAGCC
  TCAGCCACCGCAGCCAGAAGAGTGGACAGAGGATCATGCATCTGTTGTGCCTCCTGTTGCTCCTTCCCAT
  CCCCCGAGCAATCCGGAGCCAGCCAGGGAAACTCCACTTCAGGGCAAACCTTTTTTTACAAGAAACCCCT
  CTGAGCTGAAAGGCAAGTTCATTCACACGAAGCTACGGAAAAGCAGCCGAGGCTTTGGCTTCACGGTGGT
  TGGAGGAGACGAGCCTGATGAGTTCCTGCAGATCAAGAGCCTCGTCCTCGATGGTCCTGCCGCACTGGAT
  GGCAAGATGGAGACAGGGGATGTAATTGTGAGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTC
  AAGTTGTGAAAATCTTCCAGTCCATTCCCATTGGTGCCAGTGTGGACCTTGAACTCTGCAGAGGTTATCC
  ATTGCCTTTTGACCCGGATGACCCTAATACAAGTTTAGTGACCTCGGTGGCCATTTTGGACAAAGAACCA
  ATTATTGTAAATGGACAAGAGACCTACGATTCACCAGCGAGCCACAGTAGTAAAACAGGCAAAGTCAGCA
  GCATGAAGGATGCCAGGCCAAGCAGCCCTGCTGATGTGGCTTCCAACAGCTCTCATGGTTATCCCAACGA
  CACAGTCTCCTTGGCTTCCTCCATAGCCACCCAGCCAGAGCTAATAACTGTTCACATAGTCAAAGGGCCA
  ATGGGATTTGGCTTTACGATCGCAGACAGTCCCGGTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACA
  GTCCACGCTGCAGAGGCCTCAAAGAAGGGGATCTTATCGTGGAGGTGAATAAGAAGAACGTGCAGGCCCT
  GACGCACAATCAAGTCGTGGATATGCTGATTGAATGTCCCAAGGGAAGTGAGGTCACACTGTTGGTGCAG
  CGAGGAGGGCTACCAGTTCCCAAGAAGAGCCCAAAGTCGCAGCCACTGGAGAGGAAAGACAGCCAGAATA
  GCTCCCAGCACAGCGTCTCCAGCCACCGGAGCCTGCACACTGCGTCCCCGAGCCACGGCATACAGGTGCT
  CCCTGAGTACCTACCTGCAGACGCCCCTGCTCCAGATCAGACCGACAGCTCTGGGCAGAAAAAGCCAGAT
  CCTTTTAAAATCTGGGCCCAGTCCAGGAGCATGTATGAAAACCGACCTATGTCACCTTCGCCTGCATCAG
  GATTGAGCAAGGGTGAAAGAGACAGAGAAATCAATTCCACGAATTTTGGAGAATGTCAGATTCCAGATTA
  CCAGGAACAGGACATCTTCCTCTGGAGAAAAGAAACCGGATTTGGATTTAGGATTCTGGGTGGAAATGAA
  CCAGGGGAACCCATTTATATCGGTCACATCGTACCGCTGGGTGCTGCTGACACAGACGGCCGCCTGAGGT
  CTGGAGATGAATTAATCTGTGTGGATGGGACACCAGTAATTGGGAAATCACACCAGCTCGTGGTCCAGCT
  TATGCAACAAGCTGCCAAGCAAGGCCATGTCAATCTCACAGTGAGGCGGAAAGTGGTCTTTGCCGTCCCC
  AAAGCAGAGAATGAGGTGCCCTCACCAGCCTCATCACACCACAGTAGCAACCAGCCCGCGTCCCTGACGG
  AGGAGAAACGCACACCGCAAGGCAGCCAGAACTCTCTGAACACTGTGAGCTCTGGCAGCGGCAGCACCAG
  TGGCATTGGCAGTGGTGGCGGCGGGGGCAGCGGTGTGGTGAGCGCTGTGCTGCAGCCCTATGATGTGGAG
  ATTCGGCGTGGGGAGAACGAGGGCTTTGGGTTTGTCATCGTGTCCTCCGTGAGCAGACCCGAAGCGGGCA
  CAACCTTCGGCAATGCATGTGTGGCTATGCCTCACAAAATAGGTCGGATTATTGAGGGGAGCCCTGCTGA
  CCGCTGTGGCAAGCTGAAAGTAGGAGACCGGATCTTGGCAGTAAATGGATGTTCCATCACCAACAAATCC
  CATTCTGACATTGTCAACCTAATCAAAGAAGCGGGCAACACAGTGACTCTCCGCATCATCCCCGGGGATG
  AGTCCTCAAATGCCACGCTGCTGACTAATGCTGAGAAGATTGCCACCATCACCACCACTCATGCCCCCTC
  TCAGCAGGGGACCCAGGAAACAAGGACCACCACCAAACCAAAGCAGGATTCTCAGTTTGAGTTCAAAGGA
  CCGCAGGCTGCACAGGAGCAAGATTTCTACACTGTGGAATTGGAAAGAGGGGCCAAGGGATTTGGCTTTA
  GTCTTCGAGGGGGCCGAGAATATAACATGGATCTTTATGTTCTGCGCTTGGCAGAGGATGGTCCTGCAGA
  AAGATGTGGGAAGATGAGGATTGGCGATGAAATTCTAGAGATCAATGGTGAGACCACCAAAAACATGAAA
  CACTCTCGGGCCATAGAACTGATCAAGAATGGCGGCCGCAGGGTCCGTCTGTTTCTGCGGCGGGGAGACG
  GCTCAGTCCCAGAATATGGTGGGTCAAACTATGAAAACATCCCTTCCTTCCCTGGCATGACTCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286786
• Insert Size: 3708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286786.1, NP_001273715.1
• RefSeq Size: 5727 bp
• RefSeq ORF: 3708 bp
• Locus ID: 14924
• Cytogenetics: 6 D1
• Gene Summary: May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) contains multiple coding region differences compared to variant 3, and its 3' terminal exon extends past a splice site that is used in variant 3. This results in a novel 3' coding region and 3' UTR, compared to variant 3. It encodes isoform g, which is shorter and has a distinct C-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.