Nfat5 (NM_001286260) Mouse Untagged Clone

CAT#: MC229487

Nfat5 (untagged) - Mouse nuclear factor of activated T cells 5 (Nfat5), transcript variant c

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfat5
• Synonyms: AI225870; B130038B15Rik; CAG-8; CAG80; mKIAA0827; NFATL1; nfatz; OREBP; TonEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229487 representing NM_001286260
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC
  GAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGTAGCATC
  AATGAGTCAGACAAGCGGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCTGATGCTTCTTCA
  GCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATT
  CTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAG
  AGGGGTAAGTGAAAAGCAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACA
  GTTTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGT
  GTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAG
  CACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCG
  GGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTC
  ACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAG
  CCAAAAGGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAG
  GAGCTGAAGATAGTGGTACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCT
  CTGTAAAAGACAGAACACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTT
  GCAGGTATTTGTGGGCAATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACT
  GGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATC
  CTAGCAACAACATGACACTGGCGGTGGACTGTGTTGGAATATTGAAGCTGAGGAATGCCGATGTTGAAGC
  CAGAATTGGAATTGCTGGATCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACA
  AGGAAAGATGGCTCTACTCTGACATTGCAGACACCTTCTTCCCCCATTTTATGCACTCAGCCAGCAGGAG
  TTCCTGAGATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTATTTTTAATTGG
  GAAAAACTTTCTGAAAGGAACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCA
  GAAGCTGAAATTGACATGGAGTTATTCCATCAGAACCATCTTATTGTGAAGGTTCCTCCGTATCATGACC
  AACATATAACTTTGCCTGTATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCA
  GCCATTTACGTACACTCCGGATCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAAAAGGAGATATCTAGT
  CCAGCAAGACCTTGCTCTTTTGAAGAAGCCATGAAAGCAATGAAAACGACTGGATGTAACGTAGATAAGG
  TGACTATCCTTCCTAATGCCCTGATCACTCCACTCATATCAAGCAGTATGATTAAGACTGAAGATGTTAC
  TCCAATGGAAGTAACTTCAGAAAAAAGATCTTCCCCAATTTTTCAGACTACAAAGAGCATTGGATCAACC
  CAGCAAACCTTAGAAACTATTTCTAACATAGCGGGGGGTGCACCCTTCTCCTCACCGTCGTCATCTTCCC
  ACTTAACTCCTGAAAGTGAGAATCAGCAGCAGCTTCAGCCCAAGGCATACAACCCAGAGACCCTGACAAC
  TATCCAGACACAGGACATCTCACAGCCCGGGACCTTTCCCGCAGTTTCTGCTGCTAGTCAGCTGCCCAGC
  AGCGATGCACTACTGCAGCAGGCAACACAGTTTCAGACAAGAGAAGCTCAGTCCAGAGACACAATACAGT
  CAGATACAGTGGTTAACTTGTCACAGTTAACTGAAGCATCACAGCAGCAGCAATCCCCACTACAAGAACA
  AGCACAAACGTTACAGCAGCAGATACCATCAAATATTTTTCCATCGCCAAGCAGTGTCAGCCAGCTCCAG
  AGTACTATTCAGCAGCTGCAAGCAGGAAGTTTCACAGGCAGCGCTGCTGGCGGCAGGAGTGGAAGCGTTG
  ATTTGGTCCAGCAGGTTTTAGAGGCACAACAGCAGTTATCGTCTGTTCTGTTTTCTACTCCAGATGGGAA
  TGAGAATGTTCAAGAACAGCTTAATGCAGACATTTTCCAAGTCAGTCAAATTCAAAATAGTGTAAGCCCT
  GGAATGTTTTCTTCAGCGGAGTCTGCAGTTCACACTAGACCAGATAACTTACTACCTGGGAGGGCTGACA
  GCGTCCATCAACAGACTGAAAATACACTGTCTAATCAGCAGCAGCAGCAACAGCAGCAGCAGCAAGTGAT
  GGAGTCATCAGCTGCAATGGTGATGGAGATGCAGCAGAGCATTTGCCAAGCAGCTGCCCAGATCCAGTCA
  GAGCTGTTTCCTTCAGCTGCTTCAGCAAGTGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTC
  ACATGATGAGTGCACTGCCTACCAACGAGGACATGCAGATGCAGTGTGAGCTGTTCTCGTCTCCCCCTGC
  AGCTTCTGGAAACGAAACTTCCACAACCACCACGCCACAGGTGGCAACCCCTGGCTCCACCATGTTTCAG
  ACACCAAGTTCAGGAGATGGAGAAGAAACTGGAGCACAAGCAAAACAGATTCAGAACAGCGTCTTTCAGA
  CAATGGTCCAAATGCAGCGCAGTGGTGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAATAT
  AATGAGTGTTCAGAATAATGGTACCCAGCAACAAGGGAATAGCTTGTTCCAGCAAGGGAGCGAGATGATG
  TCACTTCAGTCTGGGAACTTTTTGCAGCAGTCCTCTCATTCACAGGCTCAGCTCTTTCATCCTCAAAATC
  CTATTGCTGATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCAATTTTTCATAGTCCAAATCCTATTGT
  CCACAGTCAAACTTCCACAGCATCCTCTGAACAACTGCAGCCTTCAATGTTTCACTCTCAGAATACCATT
  GCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCACCCAACATATTTCTTTCCCAAAGTTCTA
  TCAATAATCTTCAAACTAACACAGTAGCCCAAGAAGAGCAGATTTCATTTTTTGCAGCACAGAACTCAAT
  TTCTCCACTTCAGTCAACATCAAACACTGAGCAGCAAGCTGCTTTCCAACAGCAGCCTCCAATCTCACAC
  ATCCAGACCCCTATTCTTTCCCAGGAACAGGCACAACCCTCTCAGCAAGGGTTATTTCAGCCTCAGGAAA
  GCTTACACTCACACATAACCCCAGACGCTTGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286260
• Insert Size: 3678 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286260.1, NP_001273189.1
• RefSeq Size: 4725 bp
• RefSeq ORF: 3678 bp
• Locus ID: 54446
• UniProt ID: Q9WV30
• Cytogenetics: 8 53.93 cM
• Gene Summary: Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (c) uses an alternate splice site and lacks a portion of the 3' coding region, compared to variant b. This results in a shorter protein (isoform c) with a distinct C-terminus, compared to variant b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.