Adcy8 (NM_001291903) Mouse Untagged Clone

CAT#: MC229483

Adcy8 (untagged) - Mouse adenylate cyclase 8 (Adcy8), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adcy8
• Synonyms: AC8; AW060868
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229483 representing NM_001291903
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTCGGATGTGCACTGCCTTAGTGGCAGCGAGGAACTCTACACCATCCAACCGACGCCCCCGG
  CCGGCGACGACGGGAGCGGCTCTCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGGCACATCACGGA
  GCAGCGCTTCATCCACGGGCACCGAGGCGGCGGCGGCGGCGGGGTCTCCCGCAAAGCCTCGAACCCTGCG
  GGCAGTGGACCCAATCACCACGCGCCGCAGCTGTCTAGCGACTCGGTGCTGCCTCTCTATTCTCTGGGCC
  CCGGAGAGCGAGCGCACAACACCGGTGGCACCAAAGTCTTTCCGGAACGCAGCGGGAGCGGCAGTGCCAG
  TGGCAGCGGGGGCGGGGGCGACTTGGGCTTCCTACACCTTGACTGTGCCCCAAGTAACTCGGATTTCTTC
  CTCAATGGAGGATACAGCTACCGAGGGGTCATTTTTCCAACCCTACGCAACTCCTTCAAGTCTCGGGATC
  TGGAGCGCCTCTACCAACGCTATTTCCTGGGCCAGAGGCGCAAATCGGAGGTAGTGATGAACGTGTTGGA
  TGTACTAACCAAACTCACCCTTTTAGTCCTGCACTTGAGCCTGGCCTCGGCTCCAATGGACCCTCTCAAG
  GGCATCCTGCTAGGCTTTTTCACTGGCATCGAGGTGGTGATCTGCGCCCTCGTGGTGGTCAGGAAGGACA
  ACACCTCCCACACTTACCTGCAATACAGCGGCGTGGTCACTTGGGTGGCTATGACCACCCAGATTCTGGC
  AGCAGGCCTGGGCTATGGGCTTCTGGGCGACGGCATAGGCTACGTGCTTTTTACACTCTTCGCCACCTAC
  AGCATGCTTCCCCTGCCTCTCACCTGGGCCATCTTGGCCGGCCTGGGCACATCTTTGCTGCAAGTCACCC
  TTCAAGTACTCATACCCAGGCTAGCGGTCTTTTCCATCAACCAGGTCCTGGCCCAGGTGGTGCTATTCAT
  GTGTATGAATACAGCAGGAATCTTCATCAGTTACCTTTCAGACCGTGCCCAGCGCCAGGCCTTCCTGGAG
  ACCCGGAGGTGTGTGGAGGCCAGGCTCCGCTTGGAGACAGAGAACCAAAGACAGGAGCGGCTTGTGCTGT
  CTGTGCTCCCCAGGTTTGTCGTCCTGGAAATGATCAACGACATGACCAATGTGGAGGATGAGCACCTGCA
  GCATCAGTTCCACCGCATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTCAAAGGA
  TTTACCAACCTCTCTACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAACTCTTTGCCAGAT
  TCGACCGGCTGGCCCATGAGCATCACTGTCTTCGCATTAAAATCCTGGGGGACTGCTACTACTGTGTGTC
  TGGACTGCCTGAGCCCCGCCGGGACCACGCTCATTGCTGTGTTGAAATGGGCCTCAGCATGATCAAAACT
  ATCAGGTTTGTGAGATCCAGAACGAAGCACGATGTTGACATGCGAATTGGAATCCATTCAGGTTCTGTGC
  TATGTGGTGTGTTGGGCCTGAGGAAATGGCAGTTTGATGTCTGGTCTTGGGATGTGGACATAGCAAACAA
  GCTTGAGTCTGGAGGAATCCCTGGGAGGATTCACATTTCTAAAGCCACCCTGGATTGCCTCAATGGTGAC
  TATAACGTGGAAGAGGGTCACGGGAAAGAGAGGAACGAATTCTTGAGGAAGCATAACATAGAGACCTATT
  TGATTAAGCAGCCTGAGGAGAGTTTGCTCTGCTTGCCTGAAGACATCGTTAAGGAGTCGGTGAGCTGCTC
  TGACAGGAGAAACAGTGGAGCAACATTCACAGAAGGATCGTGGAGCCCAGAGCTGCCTTTCGACAACATC
  GTGGGCAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATCTCGCAC
  AAGCTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAGCATACCATCGACTTGCGGAG
  TGGCGATAAGTTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTGGAGCAC
  AAGTATTCTCAAATGAGGGATGAAGTATTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTCTGTTTA
  TCACTGCAATTCAAAGTTTGCTTCCTTCTTCAAGGCTGATGCCTATGACTATCCAGTTCTCCATCCTGAT
  CATGCTGCACTCTGCCCTGGTCCTCATCACCACAGCAGAAGACTATAAGTGTCTGCCCCTCATCCTCCGA
  AAAACCTGCTGTTGGATTAATGAGACCTATCTGGCCCGCAACGTCATCATCTTCGCTTCCATCTTAATTA
  ACTTCCTGGGAGCTGTCCTAAATATTTACTTTGTCTTCACTGGGGTGTTGGCCATGGTGACCTGTGCTGT
  GTTTCTACGGCTTAACTCTGTCCTGAAGCTGGCAGTGCTACTCATCATGATCGCCATCTACGCCCTGCTC
  ACAGAGACCATCTACGCAGGTCTCTTTCTGAGTTATGACAACCTGAACCACAGTGGAGAAGATTTCCTGG
  GGACCAAGGAAGCATCACTGCTGCTGATGGCCATGTTCCTTCTTGCTGTGTTCTACCATGGACAACAGCT
  GGAGTACACAGCCCGCCTGGATTTCCTGTGGCGCGTACAGGCCAAAGAGGAGATCAACGAGATGAAGGAA
  CTGAGGGAACACAACGAGAACATGCTGCGCAATATCTTACCCAGCCATGTGGCCCGCCACTTCCTGGAGA
  AAGACAGAGACAATGAGGAGCTGTATTCTCAATCCTACGATGCTGTTGGAGTGATGTTCGCCTCCATCCC
  TGGGTTCGCAGACTTCTACTCTCAGACAGAAATGAACAACCAGGGAGTAGAATGTCTGCGCTTGCTGAAT
  GAGATCATTGCTGACTTTGATGAGCTGCTCGGGGAGGACCGGTTTCAGGACATTGAGAAGATTAAGACCA
  TTGGTAGTACATACATGGCTGTCTCAGGACTGTCCCCAGAGAAACAGCAATGTGAAGATAAATGGGGACA
  TTTGTGTGCCCTGGCTGACTTCTCTCTTGCTCTGACTGAAAGCATACAAGAGATCAACAAGCATTCATTC
  AACAATTTTGAACTCCGAATCGGCATCAGCCATGGCTCAGTGGTGGCAGGTGTAATTGGAGCTAAGAAAC
  CACAGTATGACATTTGGGGGAAAACTGTGAACTTGGCAAGCCGAATGGACAGCACGGGAGTAAGTGGCCG
  GATCCAAGTTCCTGAGGAGACCTACCTTATCCTGAAGGACCAGGGCTTTGCCTTTGACTACCGTGGGGAG
  ATATATGTGAAGGGCATCAGTGAACAAGAAGGGAAAATCAAAACATACTTTCTCCTGGGACGAGTCCAAC
  CCAACCCATTCATCTTACCCCCAAGGAGACTTCCCGGGCAATACTCTCTGGCTGCGGTTGTCCTTGGCCT
  TGTCCAGTCTCTCAACAGGCAAAGGCAGAAGCAACTTCTCAACGAGAACAGCAATTCGGGCATCATCAAG
  AGCCATTACAACCGGCGGACTTTGCTAACGCCAAGTGGGCCAGAGCCTGGAGCACAAGCTGAAGGCACTG
  ACAAATCCGATTTGCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291903
• Insert Size: 3660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291903.1, NP_001278832.1
• RefSeq Size: 4974 bp
• RefSeq ORF: 3660 bp
• Locus ID: 11514
• Cytogenetics: 15 29.03 cM
• Gene Summary: Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion (PubMed:10864938, PubMed:25403481, PubMed:10482244, PubMed:14585998, PubMed:18448650). Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation (PubMed:10482244, PubMed:14585998, PubMed:18448650, PubMed:10864938, PubMed:12441059, PubMed:20638449, PubMed:27234425, PubMed:18222416). Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment (PubMed:25403481). Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.