Tyk2 (NM_001205312) Mouse Untagged Clone

CAT#: MC229470

Tyk2 (untagged) - Mouse tyrosine kinase 2 (Tyk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tyk2
• Synonyms: JTK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229470 representing NM_001205312
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGACCATGCCTCTGTGTGGGCGGAGAGCCATCTTGGAAGACAGCAAGGCAGATGGCACAGAAG
  CTCAGCCCCTGGTGCCCACAGGATGCTTGATGGTGCTACTGCATTGGCCTGGGCCTGAGGGCGGGGAGCC
  CTGGGTCACCTTCAGCCAGACATCTCTGACTGCAGAGGAGGTCTGCATCCACATCGCACACAAAGTCGGC
  ATCACTCCACCCTGCTTGAATCTCTTCGCACTCTACAATGCACAGGCTAAGGTCTGGCTGCCCCCAAACC
  ATATTCTGGATACATCCCAAGACATGAACCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTGGCATGG
  CATGAATCCCCAGGAGCCAGCTGTATACCGGTGTGGTTTCCCAGGGGCAGAGACTTCCTCAGACCGGGCA
  GAGCAAGGTGTACAGCTCTTGGACTCTGCCTCGTTTGAATACCTTTTTGAGCAGGGATGGTATGTTGGCA
  ATAGAGCAAAAAGCTCCCACACCTCTTTTTCTATCCATCGCTCTACCCCTCAGGGAAAGCATGAGTTCAT
  GAACGATGTGGTGTCTCTGCGGGACCTGTCTAGCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCTTA
  GGCATGGCCTTCCTGCACCTGTGTCACCTTGCTCTCAGCCGAGGCGTCCCCCTGGAGGAGATGGCCAGAG
  AGATCAGCTTCAAGAACTGCATCCCTCATTCCTTCCGACAGCACATCCGGCAGCACAATGTGCTCACACG
  CCTGCGGCTCCACAGAGTCTTCCGCCGCTTCCTGCGGGCCTTCCGGCCTGGCCACCTCTCCCAGCAGGTT
  GTGATGGTGAAGTACTTGGCTACCCTGGAGCGGCTGGCTCCGCGCTTTGGCTCAGAGCGCATACCTGTGT
  GTCATCTGGAGGTCCTGGCACAGCCCGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGACCGCTGG
  GGATCCAGGCCCAGAGCTGCCTTCCGGGCCTCCCACCCACGAGGTACTGGTGACAGGCACCGGAGGTATC
  CAGTGGCATCCGCTGCAGACCCAGGAATCTGAGAGAGGTAACAGCAGAGGGAATCCCCACGGCAGCCGGT
  CGGGGAAGAAGCCCAAAGCCCCCAAGGCTGGAGAGCACCTGACAGAGAGCCCTCAGGAACCACCTTGGAC
  CTACTTCTGTGACTTCCAGGACATTTCCCACGTGGTGCTAAAGGAGCGTCGCGTGCACATCCACCTCCAA
  GACAACAAGTGCTTGTTGCTGTGCCTCTGTTCCCAGGCTGAGGCCCTGTCCTTTGTGGCCCTGGTCGATG
  GCTATTTCCGCTTGACTGCTGACTCCAGCCACTATCTGTGCCATGAGGTGGCACCCCCGAGGCTGGTGAC
  TAGCATCCAGAACGGCATCCATGGGCCCCTGATGGATCCGTTTGTACAGGCCAAGCTGTGGCCAGAGGAC
  GGCCTCTACCTGATTCAGTGGAGCACCAGCCACCTGCACCGCCTGATCCTTACCGTGGCCCATCGAAACC
  CGGCTTTCAGTAATGGCCCTCGGGGCCTGCGCCTGCGAAAGTTCCCCATCACACAGCAGCCTGGAGCCTT
  TGTGCTGGATGGCTGGGGCCGCTCCTTTGCCAGCTTGGGGGACCTTCGGCTCGCCTTGCAGGGCTGCTCG
  TTGCGGGCCGGTGATGACTGTTTCCCCCTGCACCACTGCTGCCTGCCCCGGCCAAGAGAAATCTCCAACC
  TCGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCTCTCAACCTCAGTCAGCTCAGCTTCCACAGGGT
  TCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGAGGGCCTT
  CTAAGAGTGGGAGGCCCCGATGAGGGCAAAGTGGACAATGGATGTCCCCCTGAGCCTGGTGGGACTAGTG
  GGCAGCAGCTTCGAGTGGTGCTCAAAGTTCTCGACCCCAGTCACCATGATATCGCCTTGGCCTTCTATGA
  GACTGCTAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTTTGCGTGCGTGGC
  TCAGAGAATATCATTGTGACAGAATTCGTAGAACATGGTCCCTTGGATGTGTGGTTACGGCGACAGAGGG
  GCCAAGTGCCCATGACCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTCAGCTACCTGGAGGA
  CAAGAATCTGGTTCACGGGAATGTATGTGGCCGGAACATCCTGCTGGCTCGCCTGGGGTTGGAGGAGGGT
  ACCAACCCCTTCATCAAGCTAAGTGATCCTGGTGTGGGCCAGGGTGCCCTCTCCAGGGAAGAGCGGGTGG
  AGCGCATCCCCTGGACAGCTCCCGAGTGCCTGTCTGGAGGGACCAGTAGCTTGGGTACTGCCACGGACAT
  GTGGGGCTTTGGTGCCACCCTTCTTGAGATCTGCTTTGATGGGGAGGCACCCCTGCAGGGTCGTGGTCCC
  TCTGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCTGAACCCTCATCCCCAGAGCTGGCCACAC
  TCACCCGCCAGTGCCTGACCTATGAACCAGCACAGCGGCCATCCTTCCGCACCATTTTGCGGGACCTCAC
  AAGGCTGCAGCCACAGAATCTAGTCGGCACTTCGGCTGTGAACTCAGACTCACCAGCATCAGACCCCACT
  GTTTTCCACAAGCGCTATTTGAAAAAGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTCAGCCTGT
  ACTGCTACGACCCAACCAATGACGGCACTGGCGAGATGGTGGCCGTGAAGGCCCTGAAGGAAGGGTGCGG
  TCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAGATCCTGCGGACGCTGTACCACGAACATATTGTC
  AAGTATAAAGGCTGCTGTGAGGACCAAGGAGAGAAGTCTGTACAGCTGGTCATGGAGTACGTCCCTCTGG
  GCAGCCTCCGAGACTACCTGCCAAGGCACTGCGTAGGGCTGGCGCAGCTCCTGCTGTTTGCCCAGCAGAT
  CTGCGAGGGCATGGCCTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCCGCGCGCAACGTGCTG
  CTGGACAACGACAGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGCCACGAGT
  ACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTGTTCTGGTATGCCCCAGAATGCCTGAAGGAGTGCAA
  ATTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTAACTCTGTATGAGTTGTTGACATACTGTGACTCT
  AACCAGAGTCCCCATATGAAATTCACCGAGCTCATCGGCCATACCCAGGGCCAGATGACCGTGCTGAGGC
  TCACAGAGCTGCTGGAACGAGGAGAGAGGCTGCCTCGGCCTGACAGGTGTCCCTGTGAGATCTATCACCT
  CATGAAGAACTGCTGGGAGACAGAGGCCTCCTTCCGGCCCACCTTCCAGAATCTTGTGCCCATCCTCCAG
  ACAGCACAGGAGAAGTACCAAGGTCAGGTGCCTTCCGTGTTCAGCGTGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205312
• Insert Size: 3624 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205312.1, NP_001192241.1
• RefSeq Size: 4873 bp
• RefSeq ORF: 3624 bp
• Locus ID: 54721
• Cytogenetics: 9 A3
• Gene Summary: Involved in intracellular signal transduction by amplifying type I and type II IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. Plays an essential role in promoting selective immune responses, including innate host defense mechanisms and specific antiviral activities.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.