Wapl (NM_001301330) Mouse Untagged Clone

CAT#: MC229463

Wapal (untagged) - Mouse wings apart-like homolog (Drosophila) (Wapal), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wapl
• Synonyms: A530089A20Rik; BC037674; DIF-2; FOE; W; Wapal
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229463 representing NM_001301330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCCAGATTTGGAAAAACTTACAGTAGGAAAGGAGGAAATGGCAGTTCAAAATTTGATGAAGTTT
  TTTCCAACAAACGGACTACTCTTAGTACAAAATGGGGTGAGACCACATTTATGGCTAAATTAGGGCAGAA
  GAGGCCCAATTTCAAACCAGATATTCAAGAAATTCCGAAGAAACCTAAAGTAGAAGAAGAAGATACTGGA
  GATCCCTTTGGTTTTGATAGTGATGATGAGTCTCTACCTGTTTCTTCAAAAAATTTAGCCCAGGGTAAGG
  GTTCATCTTACTCAGAATCTAGTGAGGCTGCTCAGCTGGAAGAAGTCACTTCTGTATTTGAAGCTAATAG
  CAAATGTAGTCATGTGGTGGGTGAAGACAGTTTTGCTTCCGACAGATGCTTACTTGTGGAGGATACTTTA
  ATTGGGAAAGAGAAGAGCATAAGTAGAATTCCAGAAGACAACGCAAACAAAAGTAGTTGCACTAAGTTGC
  TAACTTCAGATAAAGTGGAGAATTTTAGTGAAGAACATGAAAAAAATAGTCACCACTTTCACAAAAATGC
  TGAAGATAGTACTAAGAAACCCAATGCAGAAACCGCAGTGGCTTCTGAATATAAAGCTGATGAAACTAAA
  GAAACAAATGATACTTGGAACTCCCAGTCTGGAAAAAGAACAGAGTCTCCATCTGAAAGTTGTCCAGTCA
  AAGGATCTGTAAGAACTGGTTTATATGAATGGGATAATGATTTTGAAGATATCAGGTCAGAAGACTGTAT
  TTTAAGTTTGGATAATGAGTCTCTTTTGGAGATGAAAGACGAGGATTTAAAAAATCGGATTGGAGGATTG
  GAAAATCTAAATGAAACCTTTGAAGAAGATATCATACAAAGTGTTCTTAGGCCAAGCAACTGTAGGACGT
  ACTGTAGGGCCAATAAAGCGAGATCCTCACAGGGAGCATCAAATTTTGATAAGCTAATGGATGGCACCAG
  TCAGTCCTTAGCCAAAGCAAACAGTGAATCAAGTAAAGATGGCCTGAATCAGGCAAAGAAAGGTAGTGCA
  AGTTGTGGGACCAGTTTTCGAGGAACAGTTGGACGGACTAGAGATTACACTGTTTTACATCCATCTTGCT
  TGTCAGTGTGTAATGTTACCATCCAGGATACTATGGAACGGAGTATGGATGAGTTCACCGCATCCACTCC
  TGCAGATTTAGGAGAGGCTGGCCGGCTCAGAAAAAAGGCAGATATTGCAACCTCCAAGACCACTACTAGA
  TTTCGACCTAGTAATACTAAATCCAAAAAGGATGTTAAACTTGAATTTTTTGGTTTTGAAGATCATGATG
  AGACAGGAGGTGATGAAGGGGGTTCTGGAAGTTCTAATTACAAAATTAAATATTTTGGCTTTGACGATCT
  CAGCGAAAGTGAAGATGATGATGATGACGACTGTCAAGTGGAAAGAAAGAAAGACAAAAAAAGAACTAAA
  ACAGCTCCATCACCTTCCCAGCAGCCTCCTCCTGAAAGCAGCGACAATTCCCAGGATAGTCAGTCTAGTA
  CTAATAATGCAGAGGACTTGCCTGGTGTGCCTGAGAGTGTGAAGAAGCCCATAAGTAAACAAGGAGATAA
  ATCCAAGGAAAATACCAGAAAGATTTTTAGTGGCCCCAAACGGTCACCTACAAAAGCTGTATATAATGCC
  AGGCATTGGAACCATCCAGACTCGGAAGAATTGCCTGGACCACCAATAGCAAAACCTCAGCGTGTCACAG
  TGAGGCTGTCTTCAAAGGAACCAAATCAAAAAGATGATGGAGTTTTTAAGGCTCCTGCACCACCACTCAA
  AGTGATAAAAACTGTGACAATACCTACTCAGCCCTACCAAGAAATAGTTACTGCACTGAAATGCAGAAAA
  GAAGACAAAGAATTATATACTGTTGTTCAGCACGTGAAACACTTCAATGATGTGGTGGAATTTGGTGAAA
  ATCAAGAGTTCACTGATGACATTGAATACTTGTTAAGTGGCTTAAAGAGTACTCAGCCTCTAAACACACG
  TTGCCTTAGTGTTATCAGCTTAGCTACTAAATGTGCCATGCCCAGTTTTCGGATGCATCTGAGGGCACAT
  GGGATGGTTGCAATGGTCTTTAAAACTTTGGATGATTCCCAGCATCATCAGAATCTGTCCCTCTGTACAG
  CTGCTCTCATGTACATATTGAGTAGAGACCGTTTGAACATGGATCTTGATAGGGCCAGCCTAGATCTCAT
  GATTCGGCTTTTGGAGTTGGAACAAGATGCCTCTTCAGCTAAGCTACTGAATGAAAAAGACATGAACAAG
  ATCAAAGAAAAGATCCGAAGACTCTGTGAAACTGTGCACAACAAGCATCTTGATCTAGAAAACATAACGA
  CTGGTCATTTAGCTATGGAGACATTGCTGTCCCTCACTTCCAAACGAGCAGGAGATTGGTTTAAAGAAGA
  GCTCCGACTTCTGGGTGGTCTGGATCATATTGTAGATAAAGTAAAAGAGTGTGTGGATCATTTAAGTAGA
  GATGATGAGGACGAAGAGAAACTAGTAGCCTCATTATGGGGAGCAGAGAGATGTTTACGAGTTTTAGAGA
  GTGTAACAGTGCATAATCCAGAGAATCAAAGCTACTTGATAGCCTATAAAGATTCACAACTCATTATTTC
  ATCAGCTAAAGCATTACAGCATTGTGAAGACCTGATTCAGCAGTACAACCGTGCTGAGAACAGCATCTGT
  GTAGCAGACAGTAACCCTCTGCCTTACCAGAATGTAACTAACCATGTGGGCAAAGCAGTGGAGGACTGCA
  TGAGGGCTATAATTGGAGTATTGCTCAATTTAACTAATGATAATGAGTGGGGCAGCACAAAGACAGGAGA
  ACAAGAAGGACTCATAGGCACAGCGATGAACTGTGTTCTTCAGGTTCCAAAGTACCTACCTCAGGAGCAG
  AGATTTGATATTCGAGTGCTGGGATTGGGTCTACTCATAAACCTGGTGGAGTATAGTGCCCGGAATCGAC
  ACTGCCTTGTCAACATGCAAACATCCTGTTCCTTTGATTCCTCCTTCTCTAGTGGAGAAGGCGATCATAG
  TTTAAGGCTAGCCGGACAAGTTCATGCTGTTCAAGCTTTAGTGCAGCTATTTCTCGAACGAGAGAGAGCA
  GCACAATTGGCAGAAAGTAAAACAGATGAATTGATTAAAGATGCTCCTACCACTCAGCATGATAAGAGTG
  GAGAGTGGCAAGAAACAAGTGGAGAAATACAGTGGGTATCAACTGAAAAGACTGATGGTGCAGAGGAGAA
  GCAGAAGAAGGAGGAGGAGGATGAAGAACTTGACCTCAATAAAGCCCTTCAGCATGCTGGCAAACACATG
  GAGGATTGCATCGTAGCCTCCTACACAGCCCTGCTTCTTGGGTGTCTCTGCCAGGAAAGTCCAATCAATG
  TAACTACAGTAAGGGAATATCTTCCAGAAGGAGATTTCTCCATAATGACAGAGATGCTTAAAAAGTTCTT
  AAGCTTCATGAATCTTACGTGTGCTGTTGGAACAACAGGCCAGAAGTCTATCTCTAGAGTGATTGAATAT
  TTGGAACATTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301330
• Insert Size: 3585 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301330.1, NP_001288259.1
• RefSeq Size: 6353 bp
• RefSeq ORF: 3585 bp
• Locus ID: 218914
• UniProt ID: Q65Z40
• Cytogenetics: 14 B
• Gene Summary: Studies suggest that the protein encoded by this gene is important for the release of cohesin from chromatin. This gene product is thought to be essential for development, and reduced expression of this gene in cells causes defects in chromatin structure. High levels of expression of the human ortholog of this gene are observed in cervical cancers, and expression of the human ortholog of this gene in mice results in tumor formation. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.