Arap1 (NM_027180) Mouse Untagged Clone

CAT#: MC229461

Arap1 (untagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arap1
• Synonyms: 2410002L19Rik; Centd2; mKIAA0782
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229461 representing NM_027180
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAGGAGGAGCCCCTGCCGAGCCGAGTCCCACGGGCTGTGCGTGTGGCTAGTCTGCTGAGCGAGG
  GGGAGGAGCTGTCTGGGGATGATTCGGAAGATGACGATGACCATGCCTATGAGGGTATCCCCAATGGTGG
  GTGGCCAACGAGTGGCCTGAATCCACCCTTACGCAGCCTGATCCCTGATCTCCCACTGCACCCCATGGAT
  GAGTTGCCTGGGGGTCCCACCCCCATCACACCTGTCATCAAGGCTGGCTGGCTGGACAAGAACCCACCAC
  AGGGGTCTTATATCTATCAGAAGCGATGGGTGAGACTGGACGCTGATTACCTGCGATACTTTGACAGTAA
  CAAGGACGCCTACTCTAAGCGCTTTGTTCCTGTGGCCTGCATCTGCCGAGTAGCTCCTATTGGAGACCAG
  AAGTTTGAAGTGATCACAAATAATCGGACCTTCGCCTTCCGGGCAGAGAGTGATGTGGAGCGGAACGAGT
  GGATGCAGGCCCTGCAGCAGGCAGTAGTTGAGCATCGAGCCCGTTTTCGGCTTTCTAGTGCTTCTGTGTT
  GGGAGTCCGAGGCTCCGAGCAGCCTGACCGTGCTGGCAGCCTGGAGCTACGAGGCTTCAAGAATAAGCTT
  TACGTGGCTGTGACTGGGGACAAAGTACAGCTCTATAAGAATCTGGAGGAATTCCATCTGGGCATTGGTA
  TCACCTTTATCGACATGAATGTGGGCAATGTCAAGGAAGTGGATCGGCGCAGCTTTGACCTCACTACCCC
  CTACCGCATCTTCAGCTTCTCGGCTGATTCAGAGTTAGAGAAGGAACAGTGGCTGGAGGCCATGCAGGGA
  GCCATCGCAGAGGCCCTATCTACCTCAGAGGTGGCTGAGCGCATCTGGGCCGCAGCCCCCAACAGGTTCT
  GTGCCGACTGTGGGGCCGCCCAGCCTGACTGGGCCTCCATCAACCTCTGCGTTGTCATCTGCAAGCGCTG
  TGCAGGGGAGCACCGCGGCCTGGGTGCAGGAGTGTCCAAGGTGCGGAGTTTGAAGATGGACAGGAAGGTG
  TGGACAGAAGCACTCATCCAGCTCTTCTTACATCTGGGCAATGGCCCCGGGAACCACTTCTGGGCTGCTA
  ATGTGCCTCCTAGTGAGGCCCTGGAGCCCAGCAGCAGCCCTGGTGCCCGGCGGTATCACCTGGAGGCCAA
  GTACAGAGAGGGCAAGTACCGCCGCTACCATCCGCTCTTTGGCAACCAAGAGGAACTGGACAAGGCCCTG
  TGTGCTGCAGTTACTACCACTGACCTGGCTGAGACCCAGGCACTCCTGGGCTGTGGGGCTGGGGTCAGCT
  GCTTCTCAGGGGACCCAGCAGCTCCCACACCCCTGGCTCTTGCTGAGCAGGCCGGACAGACTCTGCAGAT
  GGAGTTTCTACGGAATAATCAGAGTACAGAGGTCCCTCGGTTGGACTCAGTGAAGCCCTTGGAAAAGCAC
  TACTCCGTTACCCTGCCAACTGTGAGCCACAGCGGCTTCCTGTACAAAACTGCTTCTGCCGGCAAACCTC
  TGCAGGACCGCCGTGCCCGGGAAGAGTTCAGCCGGCGCTGGTGTGTCCTGAGTGATGGGGTCCTGAGCTA
  CTATGAAAACGAACGGGCAGTGACACCCAATGGGGAGATTCGGGCCAGCGAGATAGTATGCCTAGCAGTT
  TCCCCTCTGGACACCCATGGCTTTGAGCACACCTTTGAGGTGTACACAGAGGGAGAACGGCTGTACCTGT
  TTGGGCTGGAGAATGCAGAGCTGGCTCATGAGTGGGTCAAGTGCATTGCCAAGGCGTTCGTGCCTCCCCT
  GGCTGAGGACCTGCTAGCCCGGGACTTTGAACGTCTTGGGCGCCTACCCTGTAAAGCTGGCCTGAGCCTT
  CAGCAGGCTCAGGAAGGCTGGTTTGCCTTGACTGGCTCTGAGCTCCGGGCTGTCTTCCCAGAGGGGCCCT
  GGGAAGAGCCGCTGCAGCTCCGGAAACTGCAAGAGCTTTCTATCCAAGGAGACAGCGAGAACCAAGTGCT
  GGTGCTGGTGGAGCGGAGGAGGACACTGTACATCCAGGGTGAGCGGCGGCTGGACTTCATGGCTTGGCTA
  GGGGTCATCCAGAAAGCAGCAGCCAGCTTGGGAGACACACTATCAGAACAACAGCTTGGGGACTCGGACA
  TCCCAGTGATTGTGTACCGCTGTGTGGACTACATCACGCAGTGTGGCTTGACCTCAGAGGGTATCTATCG
  AAAGTGTGGTCAGACCTCCAAGACTCAGAGACTGCTAGACAGCCTCCGGCAGGACGCACGCTCTGTGCAC
  CTAAAGGAGGGAGAACAGCACGTGGACGACGTCTCCTCTGCACTCAAACGCTTCCTCAGGGACCTGCCCG
  ATGGGCTCTTCACGCGTGCGCAGCGCCTGGCCTGGCTGGAGGCCTCTGAGATCGAGGATGAGGAAGAAAA
  GATCTCCAGGTATCGAGAGCTCTTGGTGCATCTGCCCCCTGTCAACCGGGCCACTGTGAAGGCCCTTATC
  AGCCATCTGTACTGTGTACAGTGCTTCTCAGACACAAACCAAATGAACACACACAACCTGGCTATCGTGT
  TTGGGCCTACACTCTTCCAGACAGACGGGCAGGACTACAAGGCCGGCAAGGTGGTGGAAGACCTCATCAA
  CCACTACGTGGTGGTGTTCAGTGTGGACGAGGAGGAGCTGAGGAAGCAGAGGGAGGAAGTCACGGCCATC
  GTGAAGATGCGAGTGGCTGGCACTGCCAGTGGGACCCAGCATGCTGGCGACTTCATCTGCACAGTCTACC
  TGGAGGAGAAGAAGGTGGAGACTGAACAGCATGTTAAGATCCCAGCATCCATGACTGCAGAGGAGCTTAC
  TCTGGAGATTCTGGACCGCCGCAATGTGAGCATCAGGGAGAAGGACTACTGGACTTGCTTTGAGGTCAAC
  GAGAAGGAGGAGGCAGAGCGCCCGCTGCACTTTGCAGAGAAGGTGCTGCCCATTGTCCATGGGCTGGGCA
  TAGACAGCCATCTGGTGGTGAAGAAGTACCAGTCCATGGAGGCCATGCTGTTGTACTTGGCCAGCCGTGT
  GGGTGACACCAAGCATGGTATGATGAAGTTCCGTGAAGACCGCAGCCTCCTGGGTCTGGGGCTACCTTCG
  GGTGGCTTCCACGATCGCTACTTCATTCTCAACAGCAGCTGCCTACGGCTCTACAAGGAGGTCCGGAGTC
  ACCGGCCTGAGAAGGAGTGGCCTGTCAAGAGCCTCAAAGTCTACCTGGGTGTAAAGAAGAAACTGCGGCC
  ACCTACTTGCTGGGGCTTCACGGTGGTGCACGAGACAGAAAAGCACGAGAAGCAGCAGTGGTACCTCTGC
  TGTGACACGCAGATGGAACTTCGAGAGTGGTTTGCCACCTTCCTCTCTGTGCAGCACGATGGCCTGGTGT
  GGCCCTCCGAGCCATCTCGAGTGTCCCGGGCAGTGCCTGAGGTCCGGATGGGCAGTGTATCGCTGATCCC
  TCTACGAGGCAGTGAAAATGAAATGCGCCGGAGTGTGGCAGCCTTCACTGCTGACCCCCTTTCCCTTCTC
  CGCCATGTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_027180
• Insert Size: 3582 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027180.3, NP_081456.2
• RefSeq Size: 4929 bp
• RefSeq ORF: 3582 bp
• Locus ID: 69710
• UniProt ID: Q4LDD4
• Cytogenetics: 7 E2
• Gene Summary: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and lacks some 5' coding sequence, compared to variant 3. These differences cause translation initiation at a downstream AUG and an isoform (1) with a shorter N-terminus compared to isoform 3. Variants 1 and 4 encode the same protein.