Kcnma1 (NM_001253373) Mouse Untagged Clone

CAT#: MC229455

Kcnma1 (untagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 17

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229455 representing NM_001253373
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT
  ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG
  ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT
  CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA
  TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC
  TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA
  CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG
  TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT
  GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC
  ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA
  GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA
  ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGATTGAGGAAGACACATG
  GCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTCCAGTGCCTTCGTGGGT
  CTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATGATAGCCATTGAGTACA
  AGTCTGCCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGATCCAAGAAGGTACTTT
  AGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTGCAAGGCCTGTCATGAT
  GACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCCCAAGATGTCCATCTACAAGAGAA
  TGAGACGAGCATGTTGTTTTGATTGCGGACGTTCTGAGCGTGACTGCTCGTGCATGTCAGGCCGTGTGCG
  TGGTAACGTGGACACCCTTGAGAGAACCTTCCCGCTTTCTTCTGTCTCTGTTAATGATTGCTCCACCAGT
  TTCCGTGCCTTTGAAGATGAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGA
  GGAACTCGCCCAACACCTCCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGAT
  TGACAACATGGACTCCAATGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAG
  ATTGAGAAAGTCATCTTGACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCA
  TCTTTGGGGATGTCAGCTCAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTT
  TCACTATCATGAGCTCAAACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACA
  CTGCACAACTTCCCGAAAGTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCA
  ACATCAACCTCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCA
  GGACAAGGAATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTG
  CAGGCTAATTCCCAAGGATTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAGCCCAGTGCACG
  GGATGTTACGCCAGCCGTCCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTCGTGAATGATAC
  CAATGTTCAGTTTTTGGACCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTT
  GCTTGTGGGACAGCATTTGCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTTCAATGACAATA
  TCCTCACCCTAATACGGACCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGA
  GAATGCACTTCGAGGAGGCTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAG
  TTAGCCCTGTTAGATGGTCCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAG
  CTCTGAAAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAG
  CCAGTGTACAAAAAGGTACGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATC
  TTCTGCCTGATGCAGTTTGACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCT
  CACAGTCGTCCAGTAAGAAGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCC
  CAAGTCCAGGGAGTCCCGCGACAAACAGAACAGAAAAGAAATGGTTTACAGATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001253373
• Insert Size: 3555 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253373.1, NP_001240302.1
• RefSeq Size: 4962 bp
• RefSeq ORF: 3555 bp
• Locus ID: 16531
• Cytogenetics: 14 A3
• Gene Summary: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (17) has multiple differences in the coding region, one of which results in a frameshift, compared to variant 1. The resulting isoform (17) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.