Agtpbp1 (NM_001284221) Mouse Untagged Clone

CAT#: MC229443

Agtpbp1 (untagged) - Mouse ATP/GTP binding protein 1 (Agtpbp1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agtpbp1
• Synonyms: 1700020N17Rik; 2310001G17Rik; 2900054O13Rik; 4930445M19Rik; 5730402G09Rik; CCP1; nmf243
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229443 representing NM_001284221
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAGCTAAAAGTGGTGGGAGAGAAAAGCCTTACCAATAGTTCTCGGGTTGTGGGACTCCTGGCTC
  AGCTGGAGAAGATCAATACGGATTCCACAGAGTCAGATACTGCCAGATATGTTACATCCAAAATTCTTCA
  TTTGGCTCAAAGTCAAGAAAAAACAAGAAGGGAAATGACAACCAAAGGTTCTACAGGCATGGAAGTTCTG
  TTGTCAACATTGGAGAACACGAAAGATCTTCAGACTGTACTTAATATCCTAAGCATTCTCATTGAGCTGG
  TGTCATCGGGTGGGGGTCGAAGAGCGAGTTTCTTAGTTGCCAAAGGTGGTTCACAAATACTGTTGCAACT
  GCTTATGAATGCCAGCAAAGACTCTCCTCCACATGAGGAGGTGATGGTGCAGACTCACTCCATTCTTGCC
  AAGATTGGGCCAAAAGATAAGAAATTTGGAGTGAAAGCTCGAGTTAACGGGGCACTGACTGTGACTCTGA
  ACTTGGTAAAGCAGCACTTTCAGAACTACCGCTTGGTTCTTCCTTGTCTTCAGCTCTTGCGAGTCTATTC
  TACCAACTCTGTGAACTCAGTATCTTTAGGTAAAAATGGAGTTGTGGAGTTGATGTTTAAAATCATTGGG
  CCATTCAGTAAGAAGAATTCGGGTCTCATGAAGGTTGCTTTAGACACTCTTGCTGCATTGCTAAAATCAA
  AGACAAACGCCAGGAGAGCGGTGGACAGAGGGTACGTTCAAGTGCTTCTAACCATCTATGTAGATTGGCA
  CCGGCATGATAATCGGCATAGAAACATGCTCATTCGGAAGGGGATTCTACAGAGCTTAAAAAGTGTCACG
  AACATCAAGTTGGGCAGAAAAGCATTTATTGATGCGAATGGGATGAAAATTCTGTATAACACTTCTCAAG
  AGTGCTTGGCGGTCAGGACTCTTGATCCTCTTGTCAACACATCCAGTCTGATAATGAGAAAATGCTTCCC
  CAAAAACCGCCTTCCGCTCCCCACCATTAAAAGTTCTTTCCACTTCCAATTGCCAATTATCCCTGTGACT
  GGACCTGTGGCCCAGCTCTACAGCTTGCCGCCTGAAGTGGACGATGTGGTGGACGAGAGTGACGACAACG
  ACGACATTGATTTAGAAGTGGAAAATGAACTCGAGAATGAAGATGACCTAGATCAGAGTTTTAAGAATGA
  TGATATTGAAACAGATATTAATAAATTAAGACCTCAGCAAGTACCAGGACGAACAATAGAAGAACTAAAA
  ATGTACGAGCACCTTTTCCCCGAGCTTGTTGATGATTTTCAGGACTATGAACTGATCTCTAAGGAACCCA
  AACCTTTTGTGTTTGAGGGGAAGGCTCGGGGCCCCATTGTAGTTCCCACAGCTGGAGAGGAGGTACCTGG
  GAATTCAGGGAGCGTAAAGAAAGGAGTGGTAATGAAGGAGAGAGCAAGTCCTAAAGGAGAGGAAGCCAAG
  GAAGACCCTAAGGGCCACGACAGGACACTGCCGCAGCAGCTGGGTGGCCAGAGCAGAGTGGCCCCCTCAG
  CCCACAGCTTCAACAATGATCTTGTGAAGGCCCTAGACCGAATCACACTGCAGAATGTTCCTTCGCAAGT
  AGCCTCGGGCTTGAACGCAGGAATGAGGAAGGACTTTGGTCTCCCTCTCACTGTCCTCTCATGCACGAAA
  GCTTGTCCTCACGTGGCTAAGTGTGGAAGTACTCTCTTTGAAGGGCGGACGGTACATCTTGGGAAGCTGT
  GTTGTACTGGAGTTGAAACGGAAGATGACGAAGACACTGAGTCCCACTCATCAACAGAGCAGGCCCCCTC
  TGTTGAAGCCTCTGATGGACCAACACTGCACGACCCAGACCTCTATATTGAGATTGTGAAAAATACGAAG
  TCCGTTCCAGAATACTCAGAGGTGGCTTATCCTGATTATTTTGGTCACATTCCACCTCCCTTCAAAGAGC
  CTATTTTAGAAAGGCCTTATGGTGTACAAAGGACAAAAATTGCCCAAGACATCGAGAGGCTGATACACCA
  GAACGATATCATAGACCGCGTGGTGTATGACTTAGACAACCCTACCTATACCACTCCAGAAGAAGGAGAT
  ACTTTGAAGTTTAACTCAAAATTCGAATCTGGGAATCTGCGCAAAGTAATTCAAATTAGAAAAAGCGAGT
  ACGACCTTATCCTGAACTCTGATATAAACAGTAACCATTACCACCAGTGGTTCTACTTTGAAGTCAGTGG
  GATGCGGCCTGGTGTGGCATATAGGTTCAACATCATCAACTGTGAGAAGTCCAACAGTCAGTTTAATTAT
  GGTATGCAGCCACTTATGTATTCAGTTCAGGAAGCACTAAATGCCAGACCATGGTGGATCCGTATGGGCA
  CTGACATTTGTTACTACAAAAATCACTTCTCACGAAGCTCAGTTGCCGCAGGCGGACAGAAGGGCAAGTC
  CTACTACACCATCACCTTCACCGTGAACTTCCCGCACAAGGACGATGTCTGCTATTTCGCCTATCACTAT
  CCATACACGTACTCGACTCTGCAGATGCATCTTCAAAAATTGGAATCGGCACACAATCCTCAACAAATCT
  ATTTTCGAAAAGACGTGTTGTGTGAAACCTTGTCTGGAAACATCTGTCCTTTGGTGACCATAACAGCAAT
  GCCAGAGTCCAATTACTATGAACATATCTGTCAGTTCAGAACTCGCCCTTATATTTTCTTGTCTGCTCGG
  GTCCATCCTGGAGAAACCAATGCAAGCTGGGTAATGAAAGGAACACTGGAGTACCTCATGAGCAATAGCC
  CGACTGCCCAGAGCCTACGGGAGTCTTACATTTTTAAAATTGTCCCCATGCTAAATCCAGATGGTGTCAT
  CAATGGAAATCACCGCTGCTCCTTAAGTGGAGAGGATTTGAACAGACAGTGGCAAAGTCCAAACCCAGAG
  TTACACCCCACGATTTATCATGCCAAGGGGCTGCTGCAGTACCTGGCCGCGGTGAAGCGCCTACCTCTGG
  TTTATTGTGATTACCATGGCCATTCTCGAAAAAAGAATGTATTCATGTACGGCTGCAGCATCAAAGAGAC
  GGTGTGGCACACCCATGACAACTCGGCTTCCTGTGATATTGTGGAAGACATGGGATACAGGACTTTGCCT
  AAGATACTGAGCCACATTGCTCCGGCATTTTGCATGAGCAGTTGTAGCTTTGTGGTGGAAAAATCTAAAG
  AATCCACAGCTCGGGTTGTCGTGTGGCGGGAAATTGGAGTTCAGAGGAGCTACACCATGGAGAGTACTTT
  ATGTGGCTGCGATCAGGGTAGATACAAGGGTTTACAGATTGGGACTCGAGAATTGGAAGAGATGGGAGCA
  AAATTTTGTGTTGGTTTATTGCGTTTGAAACGACTGACTTCTTCATTGGAATATAATCTGCCCTCCAACC
  TGCTTGACTTTGAAAATGACTTAATTGAATCAAGCTGTAAAGTGACTAGGACACGTGGCAGCTCTGAACT
  CCAGCTTTTCCCTGCAGTCCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284221
• Insert Size: 3525 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284221.1, NP_001271150.1
• RefSeq Size: 4364 bp
• RefSeq ORF: 3525 bp
• Locus ID: 67269
• UniProt ID: Q641K1
• Cytogenetics: 13
• Gene Summary: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Deglutamylation plays a key role in cerebellar Purkinje cell differentiation, accumulation of tubulin polyglutamylation causing neurodegeneration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) has a shorter 5' UTR and uses an alternate 3'-terminal exon, compared to variant 1. The encoded isoform (4) has a shorter and distinct C-terminus, compared to isoform 1.