Pan2 (NM_001252327) Mouse Untagged Clone

CAT#: MC229442

Pan2 (untagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pan2
• Synonyms: 1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229442 representing NM_001252327
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTTTCCCCAGCTATGCATTCTACCCTGGACCCAG
  TCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTAGACCCAGAGGGAGTGGCCTTGGA
  GGCTCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCTGAGTTGCACAGCGTGGTGGCT
  GAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTACATGAGGAGATGCTGTGGGTGGGGAGCCATG
  GGGTCAATGGTGGTGACGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTCTTCCTCACCAAGAA
  CAACCTCAAGTACATGGCCCGTGGAGGGCTCATTATATTTGACTATTTGCTGGATGAAAACGAGGATATG
  CACAGCGTCCTCCTGACAGACAACAGCACTCTGCTCGTTGGGGGGCTGCAGAACCACGTACTGGAGATTG
  ACCTGAACACTGTCCAGGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGACAGAC
  AAATCGTTTCTTCTTCTGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTAAAGTG
  GAGCATGAATTTGATGCCTTCTCAGGGAGTCTGTCAGATTTTGATGTTCATGGCAACCTGCTGGCTGCCT
  GCGGCTTCTCCAGTCGCCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAAGTGTACGATCTGCGCATGAT
  GCGTGCCATCACACCTCTTCAAGTGCACGTGGATCCGGCCTTCTTACGATTCATCCCCACATACACTTCC
  CGCCTTGCTATCATCTCCCAGTCAGGTCAATGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCCAGCCG
  ACATTTTCCATGTGAATCCCGTGGGACCTTTGCTAATGACGTTTGATGTGTCAGCCAGCAAGCAGGCCCT
  GGCCTTTGGGGATTCTGAGGGCTGCGTGCATCTCTGGACTGATTCCCCTGAGCCGTCCTTCAACCCCTAC
  TCCCGAGAGACTGAGTTTGCCCTGCCCTGTCTTGTGGACTCCTTGCCGCCTCTGGACTGGAGCCAGGATC
  TGCTGCCGCTTTCCCTCATTCCCGTCCCACTTACCACTGATGCACTGCTCTCTGACTGGCCTGCTGCCAA
  CTCTGCTCCCGCTCCCAGGCGAGCACCACCTGTGGATGCAGAAATTCTTCGAACCATGAAGAAAGTGGGC
  TTCATTGGCTATGCTCCCAACCCTCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGAGTCAG
  ACCATGAATTTGACAACTTCAGCCAAGTCACGGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTCCACAC
  AGTTTCTAAGAAATACCGGAAGGTAACCATCAAATATTCCAAGCTAGGCCTGGAAGACTTTGACTTCAAA
  CACTACAATAAGACTCTGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCATGATCC
  AGGTGCTCTATTTCTTGGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAAAAGGAGTTCTGCCT
  AGCGTGCGAGCTCGGCTTCCTCTTCCACATGCTAGACCTCTCTCGTGGCGATCCTTGTCAGGGCAGTAAT
  TTCCTCCGAGCTTTCCGGACCATCCCTGAGGCCTCAGCTCTGGGTCTCATCCTTGCTGACTCAGATGAGG
  CTTCAGGCAAGGGCAGTCTGGCCCGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTACATCA
  GGATATGCAGGAGCTGGAAGTGCCCCAGGCTTATCGAGGTGCTGGAGGCAGTTTCTGCTCATCAGGAGAC
  TCCATCATTGGGCAGCTGTTCAGTTGTGAGATGGAGAACTGCAGTCTTTGCCGCTGCGGCAGCGAGACTG
  TGCGGGCCTCGTCCACCCTACTCTTCACACTCTCCTACCCTGAGGATAAAACCGGGAAGAACTATGACTT
  TGCTCAGGTGCTGAAGCGAAGTATCTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAACTGTGAGAAG
  TACCAGCCCACAATTCAGACCAGAAACATCCGACATCTGCCAGATATTCTTGTCATTAACTGTGAAGTGA
  ATAGCTCAAAAGAGGCTGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCGGTGAAAAAGTA
  CGGCGGGGAAATGAAGAGCAAAGAGTTTGCTTTAGCTGATCGGAAGGAACTGAGGAGTCCAGAGGGCTTT
  CTGTGTTCCTCCATCGAGGAGCTGAAGAATGTCTGGCTTCCATTTTCCATCCGCATGAAGATGACCAAGA
  ACAAAGGACTGGATGTTTGCAACTGGGCTGACGAGCATGAGTGGGGTCCGGCCAGGGCAGAGGAGGAGCT
  TGGTGTCTATGTATATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGAACAGGCGGCAGCTTG
  GTGGCTCACATCAAAGTCGGTGAGACCTACCACCAGCGCAAGGAGGGCGTGACTCATCAGCAGTGGTATC
  TCTTCAATGACTTCCTTATTGAACCTATTGATAAGTATGAAGCCGTGCAGTTTGACATGAATTGGAAAGT
  ACCTGCTATCCTGTATTATGTCAAAAGGAATCTCAATTCCAGATACAATCTGAATATCAAGAACCCCATT
  GAGGCCAGTGTGCTGCTGGCAGAAGCCTCGCTGGCACGGAAGCAACGGAAAACACACACGACCTTTATTC
  CATTGATGCTGAATGAGATGCCACAGGTCGGGGACCTGGTGGGCCTGGATGCCGAGTTTGTCACTCTTAA
  TGAGGAAGAAGCAGAGTTACGCAGTGATGGTACCAAGTCCACCATTAAGCCAAGCCAGATGTCAGTAGCA
  AGGATCACCTGTGTTCGGGGCCAAGGGCCTAACGAGGGCATCCCCTTCATTGATGACTACATCTCCACGC
  AGGAGCAGGTAGTAGATTACTTGACTCAGTACTCGGGGATAAAGCCAGGAGACCTTGATGCCAAAATCTC
  CTCAAAGCACCTCACAACCCTCAAGTCTACCTACTTAAAGCTTCGCTTTCTCATTGATATTGGAGTCAAG
  TTTGTGGGTCATGGTCTGCAGAAGGACTTCCGGGTCATCAACCTCATGGTGCCCAAGGACCAAGTTCTTG
  ACACAGTCTACCTGTTCCACATGCCCCGAAAACGAATGATTTCCCTACGATTCCTGGCCTGGTATTTTCT
  AGACCTGAAGATTCAAGGTGAGACCCATGACAGCATTGAGGATGCCCGCACAGCCCTTCAACTCTACCGG
  AAGTATCTGGAGCTGAGCAAGAACGGCACCGAGCCCGAGTCCTTCCACAAGGTGCTCAAGGGTCTCTATG
  AGAAGGGCCGAAAGATGGACTGGAAGGTGCCTGAGCCTGAAAGCCAGACAAGTCCCAAGAATGCAGCTGT
  CTTCTCAGTGCTGGCACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252327
• Insert Size: 3522 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252327.1, NP_001239256.1
• RefSeq Size: 4369 bp
• RefSeq ORF: 3522 bp
• Locus ID: 103135
• UniProt ID: Q8BGF7
• Cytogenetics: 10 D3
• Gene Summary: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses two different splice sites in the coding region, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.