Nrde2 (NM_001290303) Mouse Untagged Clone

CAT#: MC229441

Nrde2 (untagged) - Mouse nrde-2 necessary for RNA interference, domain containing (Nrde2), transcript variant 1


  "NM_001290303" in other vectors (1)

Reconstitution Protocol

USD 1,123.00

5 Weeks*

Size
    • 10 ug

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Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Nrde2
Synonyms 6720454P05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229441 representing NM_001290303
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGTTCCCGGCTTTTGCGGACGTAAGTGAGGCCTCCGGTGATGGGGCATTCAGGAAAGAGTTAG
ATTGGCTGAGCAACCCAAGCTTTCGTGTTGGAAATCTAACTTCTCTGAGCCGACAAACTGAAGAGGTCAC
AGCCCTTGCTTCTGAAGGGTCACCACCGCCAAGGTACTCTTTTATCAGGAGTCCTCTGAAGTCAGAGCTC
TCAGGTGAGAGCAACACTAGCGAGAAGCTCGCACAGACAAGCAGGAAGAAGAAGAAGGAGAAAAAGAAGA
GACGGAAGCATCAGCACCACAGGAAAACCAAGAGGAGGCACGAGCAGTTGAGCAGCAGCGGCTCTGAGTC
AGACACAGAGGCTGGGAAGGACAGGGCTTCCCGGAGCATCAGGGACGACCAGAAGGAAGCCGAGAAACCC
TGTCAGGGAAGCAATGCGGCTGCTGCTGTTGCTGCTGCTGCTGGACATCGGTCCATTTGGCTGGAAGATA
TTCACGATCTGACCGACGTCTTCAGGACTGATAAGAAGCCAGATCCTGCCAACTGGGAGTATAAATCTCT
GTATCGTGGGGATATAGCAAGGTACAAGAGGAAGGGAGACTCCTGCCTGGGTATCAATCCTAAGAAACAG
TGTATATCTTGGGAAGGGGCTTCTGCAGCAAAGAAGCACTCACACAGGCACCTGGAGCGCTATTTTACCA
AGAAGAATGTGGGGTTAATGAGAACAGAGGGCATTGCTGTGTGCAGTAACCCCGAGCCTGCCTCCTCCGA
GCCCGTCACATTCATCCCAGTGAAGGACTCTGCTGAGGCAGCTACTCCTGTTACATCATGGCTGAATCCT
CTGGGGATTTATGATCAGTCTACCACACAGTGGTTACAAGGACAGGGTCCAGCAGAGCAAGAGTCAAAGC
AGCCGGACTCGCAACAGGACAGGGAGAATGCTGCTCTCAAGGCCAGAGTGGAAGAGTTTAACAGAAGGGT
TCGGGAGAATCCTTGGGACACGCAACTATGGATGGCGTTTGTGGCTTTCCAGGACGAGGTCATGAGGAGT
CCTGGCATCTATGCCCTTGGGGAAGGAGAACAGGAGAAGCACAGGAAGTCCCTGAAGCTCCTCCTGGAGA
AGAAGCTGGCTGTGCTGGAGCGGGCCATCGAGAGCAACCCAGGCAGCGTGGAGCTGAAGCTTGCCAAGCT
GCAGCTGTGCTCAGAGTTCTGGGAGCCCAGTGCGCTGGCCAAGGAGTGGCAGAAGCTCCTCTTCCTGCAC
CCCAACAACACAAGCCTGTGGCAGCGCTATCTCTCCTTTTGCCAGAGCCAGTTTGGTACCTTTTCTGTGT
CCAAACTCCACAGTCTGTATGGCAAGTGCTTGAGTACCTTGTCTGCTGTTAAAGATGGCAGCATGCTATC
CCACCCTGTGTTGCCGGGCACCGAGGAGGCCATGTTTGGGCTCTTTCTTCAGCAGTGCCACTTTCTACGG
CAGGCTGGCCACTCAGAGAAGGTCATCTCTTTGTTCCAGGCCATGGTTGACTTCACCTTCTTCAAGCCTG
ACAGTGTGAAAGAGCTGCCTACTAAAGTACAGGTGGAATTCTTTGAGCCCTTCTGGGACAGCGGAGAACC
ACGCGTTGGGGAGAAGGGAGCCCGAGGCTGGCGGGCGTGGATGCACCAACAGGAGCGAGGTGGCTGGGTG
CTCATCACCCCAGATGAGGATGACGAGGAGCCCGAAGAGGAGGACCAGGAGATAAAAGACAAGACCCTGC
CCCGGTGGCAGATCTGGCTTGCTGTTGAGCGTTCTCGAGACCAGAGGCACTGGCGTCCCTGGCGCCCTGA
TAAGACCAAGAAGCAAACGGAGGAAGACTGTGAGGACCCTGAGAGACAGGTGCTGTTTGATGACATTGGG
CAATCTTTGATCAGACTTTCCAGCCCAGACCTTCAGTTCCAGCTCATCCAGGCCTTTCTGCAGTTCCTGG
GCGTGCCTTCCGGCTTCCTGCCCCCAGCCTCCTGCCTTTACCTGGCCATGGACGAGAGCAGCATCTTTGA
GAGTGAACTTTATGATGAAAAGCCCCTGACTTACTTCAACCCTTCCTTTTCGGGCATTAGCTGTGTCGGC
TCCATGGAACAGTTGGGTCGCCCTCGCTGGACCAAAGGTCACAACCGAGAGGGCGAGGAGTTTGTCCGCA
ATGTCTTCCACCTGGTGCTGCCTTTGCTCGCAGGCAAGCAGAAGTCCCAGCTCAGCCTCTCCTGGTTACG
GTACGAGATTGCAAAGGTCATTTGGTGTCTACACACTAAAAAGAAGCGATTGAAGTCTCAGGGAAAGAGC
TGCAAAAAACTAGCCAAGAATCTCCTCAAGGAGCCAGAAAACCGCAACAACTTCTGCCTCTGGAAGCAGT
ATGCACACCTGGAGTGGTTGCTCGGTAACACAGAGGATGCCAGAAAAGTGTTCGACACAGCACTCAGCAT
GGCAGGCAGCAGCGAGCTGAAGGACCGTGAACTCTGCGAGCTCAGTCTGCTCTATGCCGAGCTGGAGATG
GAACTGTCGCCGGACTCGAGAGGAGCCACCACAGGCCGAGCGGTCCACATATTAACACGGCTGACGGAGA
GCAGTCCCTACGGGCCCTACACGGGGCAGGTCTCGTCCACCCAGGTGCTGAAAGCTCGGAAGGCTTACGA
GCTCGCGCTGCAGGACTGCCTGGGCCAGAGCTGTGCCTCCAGCCCAGCTCCTGCAGAGGCCTTGGACTGC
CTGGGTAGCTTGGTCCGATGCTTTATGCTCTTCCAGTATTTGACTGTGGGCATTGATGCTGCTGTGCAGA
TATACGGAAGGGTGTTTGCCAAGCTGAAGGGCTCTGCTCGCTTAGAAGACCCAGGGCCAGAGGACAGCAC
CAGCTCCCAGAGTTTGACCAATGTGCTCGAGGCTGTCAGCATGATGCACACAAGCCTGCTGCGATTCCAC
ATGAACGTTTGTGTTTACCCTCTGGCTCCACTCCGAGAGACCCTCTCCGATGCTTTAAAGCTGTATCCGG
GCAACCAGGTTCTTTGGAGGGCTTATGTACAGATTCAGAATAAGTCCCACAGTGCTAACAAGACCAGAAG
GTTCTTTGACACAGTTACTAGGTCTGCCAAACATTTGGAGCCTTGGCTATTTGCAATCGAAGCTGAGAAG
CTGAGGAAAAAGCTAGTGGAATCTGTTCAGAGGGTAGGAGGCAGAGAGGTCCATGCAACAATCCCCGAGA
CTGGCCTTACGCATCGCATCAGAGCTTTGTTTGAAAATGCGATTCGGAGCGACAAGGGCAACCAGTGTCC
CCTACTGTGGAGGATGTATTTGAATTTTTTGGTTTCCTTAGGAAACAAAGAAAGAAGCAAAGGCGTGTTC
TACAAGGCCCTCCAGAGCTGTCCTTGGGCAAAGGTGCTATACATGGACGCCATGGAGTATTTCCCAGACG
AGCTGCAGGAGATCCTGGACGTGATGACCGAGAAGGAGCTCCGTGTGCGCCTGCCCCTTGAGGAGCTGGA
GCTGCTGCTGGAGGATTAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001290303
Insert Size 3519 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001290303.1, NP_001277232.1
RefSeq Size 3725 bp
RefSeq ORF 3519 bp
Locus ID 217827
UniProt ID Q80XC6
Cytogenetics 12 E
Gene Summary Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation. Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation. Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome. By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). The exon combination of this variant is inferred based on tiled partial transcript alignments. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The exon combination of this CCDS representation is inferred due to a lack of full-length transcript support for this gene. It is supported by tiled partial transcript alignments.

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