Hipk2 (NM_001294143) Mouse Untagged Clone

CAT#: MC229436

Hipk2 (untagged) - Mouse homeodomain interacting protein kinase 2 (Hipk2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hipk2
• Synonyms: 1110014O20Rik; B230339E18Rik; Stank
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229436 representing NM_001294143
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTG
  CCTTCTGTAGTGTGAAGAAACTAAAAGTAGAGCCAAGTTCCAACTGGGACATGACTGGGTACGGCTCCCA
  CAGCAAAGTGTACAGCCAGAGCAAGAACATACCACCTTCTCAGCCAGCCTCCACAACCGTCAGCACCTCC
  TTGCCAATCCCCAACCCAAGCCTACCTTACGAGCAGACCATCATCTTCCCAGGAAGTACTGGACACATAG
  TTGTAACATCAGCAAGTAGCACTTCTGTCACCGGGCAAGTCCTCGGTGGACCACATAACCTAATGCGTCG
  AAGCACTGTGAGCCTCCTTGACACCTACCAAAAATGTGGACTCAAGCGCAAGAGTGAGGAGATTGAGAAC
  ACAAGCAGCGTGCAGATCATTGAAGAGCATCCACCCATGATTCAGAATAATGCCAGCGGGGCCACTGTAG
  CCACTGCCACCACATCCACTGCCACCTCCAAAAACAGTGGCTCCAACAGTGAAGGGGACTATCAGCTGGT
  GCAGCATGAAGTACTGTGCTCCATGACCAACACCTATGAGGTTTTAGAGTTCTTGGGGCGAGGGACATTT
  GGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAAATTGTGGCCATTAAGATCCTCAAGAACCACC
  CCTCCTATGCCCGGCAAGGCCAGATTGAAGTGAGCATCTTGGCCCGGCTGAGCACGGAGAGCGCTGATGA
  CTACAATTTTGTGCGGGCCTATGAGTGCTTCCAGCACAAGAACCACACGTGCTTAGTCTTTGAGATGTTG
  GAGCAGAACCTTTATGACTTTCTGAAACAGAACAAGTTTAGTCCCTTGCCCCTCAAGTATATTCGCCCAG
  TCCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTGGGTCTTATCCATGCTGACCTCAAACC
  AGAAAACATCATGCTGGTGGATCCATCCAGACAACCGTACCGAGTGAAGGTCATTGACTTTGGTTCAGCT
  AGTCATGTGTCCAAAGCTGTCTGCTCTACGTACTTGCAATCCAGATACTACCGGGCCCCTGAGATCATCC
  TTGGTTTGCCATTCTGTGAGGCAATTGACATGTGGTCCCTTGGTTGTGTCATTGCTGAACTGTTCCTGGG
  CTGGCCATTATACCCAGGTGCTTCTGAGTATGATCAGATTCGGTATATTTCACAAACACAGGGTTTACCA
  GCTGAATATTTATTAAGTGCAGGGACAAAGACAACTAGGTTTTTCAACCGTGACACAGACTCACCGTATC
  CTTTGTGGAGGCTAAAGACACCAGATGACCATGAAGCAGAGACGGGAATTAAGTCAAAGGAAGCAAGAAA
  GTACATTTTCAACTGTTTGGATGATATGGCCCAGGTGAACATGACAACAGATTTGGAAGGGAGTGACATG
  TTAGTAGAGAAGGCAGACCGACGGGAGTTCATTGACCTGTTAAAGAAGATGCTGACCATCGATGCTGATA
  AGAGAGTCACTCCCATTGAAACTCTGAACCACCCCTTTGTCACCATGACACACCTGCTTGACTTCCCCCA
  TAGTGCCCATGTCAAATCTTGTTTCCAAAACATGGAGATTTGCAAGCGCCGGGTGAATATGTATGACACA
  GTGAACCAGAGCAAAACACCTTTCATCACTCACGTGGCCCCCAGCACATCCACCAACTTGACCATGACCT
  TTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATGGCCGCGGTGGCCCCGCGGAG
  CATGCCCCTGCAGACGGGAACAGCCCAAATTTGTGCCCGACCTGATCCCTTCCAGCAAGCTCTCATCGTG
  TGTCCCCCTGGCTTCCAAGGCCTGCAGGCCTCTCCCTCCAAGCACGCTGGCTACTCAGTGCGGATGGAAA
  ATGCTGTCCCCATCGTCACCCAGGCGCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGCCTGCTTGCTCA
  GCAGGCCTGGCCAGGTGGGGCCCAACAGATCCTACTTCCTCCAGCATGGCAGCAGCTGACTGGCGTGGCC
  ACCCACACATCTGTACAGCATGCAGCTGTGATTCCTGAGACCATGGCAGGCACTCAGCAACTGGCTGACT
  GGAGGAACACGCATGCTCATGGAAGTCATTACAATCCCATCATGCAGCAGCCTGCACTCTTGACTGGTCA
  TGTGACCCTTCCAGCTGCCCAGCCCTTAAATGTGGGTGTAGCCCATGTGATGAGGCAACAACCAACCAGC
  ACCACCTCCTCCAGGAAGAGTAAGCAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGACCT
  CTTCACAGGCTATCAGCTCCCCTCAGCGATCCAAGCGTGTCAAGGAGAACACTCCCCCACGGTGCGCCAT
  GGTACACAGCAGCCCAGCTTGCAGCACCTCAGTCACCTGTGGGTGGGGCGACGTGGCCTCCAGCACCACC
  CGGGAGCGACAGCGGCAGACGATTGTCATCCCCGACACCCCGAGCCCCACAGTCAGTGTCATCACCATCA
  GCAGTGACACCGATGAAGAAGAGGAGCAGAAGCACGCCCCCACCAGCACAGTCTCCAAGCAAAGAAAAAA
  TGTCATCAGCTGTGTCACCGTCCACGACTCTCCCTACTCTGACTCCTCCAGCAACACCAGCCCCTACTCG
  GTGCAGCAGCGCACAGGGCACAACGGCACCAACACCTTGGACACCAAGGGGGGCCTAGAGAATCACTGCA
  CTGGCAACCCCCGCACCATCATCGTACCCCCACTGAAGACCCAGGCCAGTGAAGTGTTGGTAGAATGTGA
  CAGCCTAGGGCCAGCGATCAGTGCCAGTCACCATTCGTCCTCCTTCAAGTCCAAGTCCTCCAGCACCGTG
  ACCTCCACCAGTGGACACTCTTCAGGGAGCTCTTCAGGAGCCATCGCCTACCGTCAGCAGCGGCCAGGCC
  CCCACTTCCAGCAGCAGCAGCCCCTCAATCTCAGCCAGGCCCAGCAGCACATGGCTGCGGACCGCACCGG
  GAGTCACCGTCGACAGCAGGCCTACATCACTCCGACCATGGCGCAAGCTCCGTACACCTTCCCGCACAAC
  AGCCCCAGCCATGGCACTGTTCACCCCCACCTGGCTGCGGCTGCCCACCTCCCCACCCAGCCTCACCTCT
  ACACCTATACAGCGCCCACAGCCCTAGGCTCCACCGGCACTGTAGCCCACCTGGTGGCATCCCAAGGCTC
  AGCACGCCACACCGTGCAGCACACTGCCTATCCGGCCAGCATCGTCCACCAGGTCCCTGTGAGCATGGGG
  CCCCGGGTCCTGCCCTCGCCCACCATCCACCCCAGTCAGTATCCAGCCCAGTTTGCCCACCAGACCTATA
  TCAGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGTCCTGCCAAGGTCAACCAATACCC
  TTACATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001294143
• Insert Size: 3510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001294143.1, NP_001281072.1
• RefSeq Size: 4178 bp
• RefSeq ORF: 3510 bp
• Locus ID: 15258
• UniProt ID: Q9QZR5
• Cytogenetics: 6 B1
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region compared to variant 2. The encoded isoform (3) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.