Agap2 (NM_001301014) Mouse Untagged Clone

CAT#: MC229432

Agap2 (untagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agap2
• Synonyms: AGAP-2; Centg; Centg1; cnt-g1; mKIAA0167; PIKE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229432 representing NM_001301014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGGGCGCGGGCGCGCTTCAGCGCCGGACAACGACCTACCTCATCTCGCTGACCCTGGTCAAGC
  TCGAGTCGGTACCTCCGCCGCCGCCTTCTCCATCTGCAGCCGCAGCCGGCGCCCCCGGGGCCAGAGGCTC
  CGAGCCCCGAGATCCTGGCAGCCCCCGAGGCTCGGAGGAACCCGGCAAGAAGCGGCACGAGCGTCTCTTC
  CACCGGCAGGATGCGCTGTGGATCAGTACCAGCAGCGCAGGCACCGGGGGTGCGGAGCCCCCCGCGCTGT
  CCCCGGCTCCGGCCAGTCCGGCTCGCCCGGTCTCCCCGGCTCCGGGCCGCCGACTGTCCCTATGGGCCGC
  CCCTCCGGGACCCCCGCTCTCCGGGGGGCTGAGCCCGGACTCCAAGCCGGGAGGCGCCCCCTCTTCCTCC
  CGGCGCCCTCTACTCAGCAGCCCGAGCTGGGGGGGTCCGGAACCTGAAGGCCGGACGGGTGGCGGAGTCC
  CGGGCTCGTCCTCCCCGCATCCTGGCACCGGTAGCCGAAGGCTCAAGGTGGCGCCTCCTCCACCTGCTCC
  CAAGCCTTTCAAGACCGTGACCACTAGTGGAGCCAAAGCCGGCGGGGGCAAGGGCGCAGGCAGCCGCCTG
  TCATGGCCCGAAAGCGAGGGCAAACCCAGGGTCAAGGGGTCAAAGAGCACCGCCGGGACTGGAGCTTCTG
  CCGCTGCGGCCGGCGGGGGAGGGAGCGCTGCAGTCACGACCTCTGGTGGGGTCGGGGCTGGGGCTGGAAC
  CAGAGGGAAGTTGTCCCCTCGGAAAGGCAAGAGTAAGACCTTGGACAATAGTGACTTACACCCAGGACCC
  AGTGCCGGCTCTCCTCCGCTCACCGTGCCAGCAATCCCAGTTCCAGCCACTTCTGTCACTGCCACCTCCA
  CGCAGCCCCTCGGGCCTGCACCCCCTATCACTCTGGAGCCACCAGCTCCAGGGCTGAAAAGGGGCCGGGA
  GGGCGGCCGAGCGTCCACTCGTGACCGCAAGATGCTCAAGTTTATCAGCGGTATCTTCACCAAGAGCACA
  GGAGGGCCTCCTGGTCCAGGGCCCCTTCCCGGACCCCAAGGCTTGTCTTCCAGCAGCGGGTCCAGGGAGC
  TGCTGGGAGCAGAACTACGCACCTCCCCTAAGGCTGTGGTTAATAGCCAGGAATGGACTTTGAGCCGATC
  AATTCCCGAACTGCGCCTGGGTGTGCTGGGTGATGTCAGGAGTGGGAAGTCGTCCCTCATCCATAGATTC
  CTCACGGGTTCATACCAGGTGCTGGAGAAGATTGAGAGTGAGCAGTACAAGAAGGAGATGCTAGTGGATG
  GGCAGACCCATCTGGTGCTGATCCGGGAGGAAGCAGGGGCACCCGATGCCAAGTTCTCAGGCTGGGCAGA
  TGCTGTGATCTTCGTCTTCAGCCTGGAGGATGAGAGCAGCTTCCAGGCTGTGAGCCGTCTCCATGGGCAG
  CTGAGCTCCCTCCGGGGGGAGGGGCGAGGAGGTCTGGCCCTGGCTCTGGTTGGGACCCAAGACAGGATCA
  GTGCATCATCTCCTCGAGTGGTGGGTGATGCTCGAGCCAGGGCTCTGTGCACGGACATGAAGCGCTGTAG
  CTACTACGAGACCTGCGCAACCTATGGGCTCAATGTGGACCGGGTCTTCCAGGAGGTGGCCCAGAAGGTG
  GTGACCTTGCGCAAACAACAACAACTCCTGGCTGCTTGCAAATCCCTGCCTAGCTCGCCAAGCCATTCAG
  CTGCATCTACTCCTGTAGCTGGGCAGGCTAGTAACGGGGGCCACACTAGCGACTACTCTTCTTCCCTCCC
  ATCCTCACCCAACGTTGGACACCGGGAGCTCCGAGCTGAGGCAGCTGCGGTGGCTGGGTTGAGTACCCCA
  GGGTCCTTACACAGAGCAGCCAAGCGGCGGACCAGCCTTTTTGCGAACCGTCGGGGCAGCGATGCTGAGA
  AGCGGAGCTTAGACAGCCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCT
  GCTAAAACGAAGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTGACCTTGTCCAGCAATGGC
  TTTCTACTCTACCACCCCAGCATTAATGACTACATCCACAGTACACATGGCAAAGAGATGGACTTGTTAC
  GAACAACGGTCAAAGTCCCAGGCAAGCGGCCCCCAAGGGCTATCTCTGCTTTCGGCCCCTCAGCCAGCAT
  CAACGGGTTGGTCAAGGATATGAGCACCGTCCAGATGGGTGAAGGCCCTGAAGCCTCTACTCCCATGCCA
  AGCCCCAGCCCTAGCCCCAGTTCCCTGCAGCTTCCAACAGACCAGACATCCAAGCACCTCTTGAAGCCAG
  ACCGGAATTTGGCCCGTGCCCTTAGCACCGACTGTACCCCATCTGGAGATCTGAGTCCCCTGAGTCGGGA
  ACCCCCACCTTCGCCCATGGTGAAGAAGCAGAGGAGGAAGAAACTGTCCACGCCTTCTAAGACTGAAGGC
  TCGGCTGTGCAAGCTGAAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACTGGTCAGACGTGGCACT
  TTGAGGCAGCCAGCTTTGAGGAGCGGGATGCTTGGGTTCAGGCCATTGAGAGTCAGATCCTGGCCAGTCT
  GCAGTGCTGTGAGAGCAGCAAGGTCAAGCTGCGCACTGACAGCCAAAGCGAAGCTGTGGCCATCCAGGCG
  ATCCGCAACGCAAAGGGAAACTCGACCTGTGTGGACTGTGGAGCTCCCAACCCCACGTGGGCCAGTTTGA
  ATCTGGGCGCACTCATCTGCATCGAGTGTTCTGGCATCCACCGGAACCTGGGCACACACCTGTCCCGAGT
  GCGTTCACTGGATTTGGATGACTGGCCGCGGGAGCTGACCCTGGTGCTGACGGCCATTGGCAACGACACA
  GCCAACCGCGTGTGGGAGAGCGACACTCGAGGCCGTGCCAAGCCTACACGGGACTCTTCGCGGGAGGAGC
  GTGAGTCGTGGATTCGCGCCAAGTATGAGCAGCTGCTGTTCCTGGCGCCTCTGGGCACCACGGAGGAGCC
  GCTGGGCCGCCAACTGTGGGCCGCTGTGGAGGCCCAGGACGTGGCCGCCGTTCTGCTGCTTCTGGCCCAC
  GCGCGACATGGGCCGCTCGACACCAGCGTGGAGGACCCGCAGCTCCGCTCGCCGCTGCACTTGGCGGCCG
  AGCTCGCCCACGTTGTCATTACACAGCTGCTGTTGTGGTATGGCGCAGACGTGGCGGCCCGCGATGCGCA
  AGGCCGCACGGCACTGTTCTATGCCCGCCAGGCTGGCAGCCAGCTGTGTGCAGACATCCTTCTCCAGCAC
  GGCTGCCCGGGAGAGGGCGGCAGCACGGCCACCACGCCCAGCGCGGCCACCACCCCCAGCATTACCGCCA
  CGCCCAGCCCGAGGCGCCGGAGCAGTGCTGCCAGCTTGGGCCGCGTGGACACCACGATCGCGCTGGTATA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301014
• Insert Size: 3501 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301014.1, NP_001287943.1
• RefSeq Size: 5585 bp
• RefSeq ORF: 3501 bp
• Locus ID: 216439
• UniProt ID: Q3UHD9
• Cytogenetics: 10 D3
• Gene Summary: The protein encoded by this gene is a member of the centaurin GTPase family. This gene product regulates the activity of multiple kinases, including PI3K. Reduced expression of this gene results in multiple defects, including neural deficiencies, while increased expression of this gene has been observed in some tumors. Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.