Plcb2 (NM_001290790) Mouse Untagged Clone

CAT#: MC229422

Plcb2 (untagged) - Mouse phospholipase C, beta 2 (Plcb2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plcb2
• Synonyms: AI550384; B230205M18Rik; B230399N12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229422 representing NM_001290790
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTGCTCAACCCTGTTCTATTGCCCCCTAACGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA
  TCAAGTGGGACGATGAAACTTCAATAGCCTCTCCTGTTATCCTCCGTGTGGATCCCAAGGGCTATTACTT
  ATACTGGACATATCAAAATCAGAGCCAGAAACTCCGGGAGGTCTTCAACATGGACTTTCCAGACAACCAC
  TTCCTGCTGAAAACACTCACAGTGGTGTCCGGCCCTGACATGGTGGACCTCACCTTCTACAACTTTGTCT
  CTTACAAGGAGAACGTAGGCAAGGACTGGGCTGAGGACGTGCTAGCTCTCGCCAAACACCCGATGACAGT
  CAATGCTCCTCGAAGCACATTCCTGGACAAGATCCTGGTGAAGCTTAAGATGCAGCTCAACCCTGAAGGG
  AAGATTCCCGTGAAGAATTTTTTCCAGATGTTTCCTGCTGATCGAAAACGGGTGGAAGCTGCCCTCGGTG
  CTTGTCACCTTGCAAAAGGCAAAAATGATGCTATCAACCCAGAGGACTTCCCAGAATCCGTGTACAAGAG
  CTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATCGACGAGATCTTCACTTCCTACCACTCTAAAGCTAAG
  CCCTACATGACCAAGGAACACCTGACCAAATTCATCAATCAGAAGCAGCGAGACCCTCGACTCAACTCCT
  TGCTGTTCCCACCAGCCCGGCCTGAGCAAGTTCAAGTGCTCATTGACAAGTACGAACCCAGCGGCATCAA
  TGTGCAGAGGGGCCAGCTGTCACCAGAGGGGATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTGCTG
  GCCCACGATACACTGCTGATCCACCAAGACATGACACAACCACTGAATCACTATTTCATCAACTCCTCAC
  ACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCT
  GTCTGGCTGCCGGTGTGTAGAGTTAGACTGTTGGAAGGGCAAGCCCCCTGACGAAGAGCCCATTATCACC
  CACGGCTTCACCATGACCACAGATATCTTGTTCAAGGAAGCAATTGAAGCCATTGCAGAAAGTGCCTTTA
  AGACCTCCCCGTATCCTGTCATCCTATCATTTGAAAACCATGTAGACTCACCCCGGCAACAGGCTAAGAT
  GGCTGAGTACTGCAGGAGCATGTTTGGAGAGACCTTGCTCACAGATCCCCTGGAAAATTTCCCTCTGAAA
  CCTGGCATCCCTCTGCCCAGCCCCGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGT
  TTTCTGGGCCAGCATCCCCCAGCAAGAAGCCTGGTGGGGTGGCTGAGGGCAGCCTCCCGTCTAGTGTCCC
  TGTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGAGGAGGTGGTGGAAGAG
  GAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCGGATGAGGGCACAG
  CAGGCCTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTATATCCAGCCCACCAAGTTTAT
  CTCCTTCGAGTTCTCTGCACAGAAGAACAGAAGTTACGTTGTCTCTTCCTTCACGGAGCTCAAGGCCTAC
  GAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGTGTGTACCCCA
  AGGGCACACGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGTCAGATGGTTGC
  CCTCAATTTCCAGACAATGGACCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACGGGCAGAGT
  GGCTACCTCCTAAAGCACGAGTTCATGCGCAGACTGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCA
  TTGATGTGGTGGTAGCCACCACCCTTTCCATTACGATCATCTCTGGGCAGTTCCTGTCAGAGCGCAGTGT
  ACGCACCTATGTGGAAGTGGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGGTATCGAACCAAGCTG
  TCACCTACTGCTAACTCCATCAATCCTGTCTGGAAAGAGGAACCCTTTATCTTTGAGAAGATCTTGATGC
  CTGAGCTGGCCTCCCTCAGGATAGCTGTGATGGAAGAAGGCAGCAAATTTCTTGGACACCGCATCATCCC
  CATCAATGCCTTACATTCTGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGGCCCTTACCATG
  CCAGCTCTCTTTGTCTTCCTGGAGATGAAGGACTACATACCTGACACCTGGGCAGATCTTACAGTAGCCC
  TTGCCAACCCCATCAAGTACTTCAATGCCCAGGATAAGAAGTCCGTGAAGCTCAAAGGAGTGACAGGAAG
  TCTGCCCGAGAAACTCTTCTCTGGGACACCTGTTGCCAGTCAGTCCAATGGGGCGCCAGTCTCGGCAGGC
  AATGGGTCAACAGCGCCTGGGACCAAGGCCACGGGGGAAGAAGCTACGAAAGAAGTGACAGAGCCACAGA
  CCGCCAGCTTGGAAGAGTTGCGGGAACTGAAGGGCGTGGTGAAACTGCAGCGAAGACATGAGAAGGAGCT
  TCGAGAGTTGGAGCGCCGTGGAGCCCGGCGCTGGGAGGAACTGCTGCAGCGTGGCGCCGCACAGCTGGCG
  GAGCTCCAGACCCAGGCAGCAGGCTGCAAGCTCCGCCCAGGCAAGGGCTCCCGCAAGAAGAGGACCCTGC
  CCTGTGAGGAGACCGTCGTGGCGCCTAGCGAGCCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAA
  GGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCCGTCCTCAAGCGCAAGGAG
  CAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACTCAAGA
  CCTTCAAGGAGACCTCAGAAACTGACACCAAAGAAATGAAGAAAAAACTGGAGGCCAAGAGGCTGGAACG
  GATCCAAGCCATGACCAAGGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTAACAAC
  TCCCACATCCAGGAAGTGGTCCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGAGAAGC
  TGGAAGAGAGGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAGGCGCT
  GGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCGGTGCGGGCCTACTTCAAG
  GACTGCTTCCCCACCGAAGCAGAAGACAAGCCTGAGAGGTCCTGTGAGGCCTCTGAGGAGTCGTGTCCAC
  AGGAACCACTTGTGAGCAAGGCAGACACTCAGGAGAGCCGCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290790
• Insert Size: 3477 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290790.1, NP_001277719.1
• RefSeq Size: 5091 bp
• RefSeq ORF: 3477 bp
• Locus ID: 18796
• UniProt ID: A3KGF7
• Cytogenetics: 2 59.43 cM
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.