Ciita (NM_001302618) Mouse Untagged Clone

CAT#: MC229420

Ciita (untagged) - Mouse class II transactivator (Ciita), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ciita
• Synonyms: C2t; C2ta; EG669998; Gm9475; Mhc2ta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229420 representing NM_001302618
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCAGGCAGGTGC
  GGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATGA
  GCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACT
  TTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATG
  GAGACCATAGTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAG
  TGATGCCGACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAA
  CTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAAC
  TGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCA
  GCTCGAGGGCCTGAGCAAGGACCTCTTCATAGAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATA
  GAGATCCCTGTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAA
  AGCACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCA
  GATCTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTA
  ATCTACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCC
  CCGAGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCC
  CGAACCTGCGCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCC
  GAGTCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAAT
  ACAAGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAG
  AGGCAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGT
  GGTCTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGC
  TGGGCAAGGCTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTT
  GCTACAATATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTG
  CGGGATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGA
  GGCAGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCA
  CGGACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGG
  AAGCTACTGCGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCA
  AGGCAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTT
  TGAGAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGC
  AGCTATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCA
  CAGAGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAG
  TCCTCCCGGAGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTG
  CAAGAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCC
  TGGAGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCT
  GGCACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGA
  CCCTATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACT
  GCCTGGGAGCTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTA
  CCTGAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAG
  ACACAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCC
  GGCTCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGA
  CCTTCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTC
  AGGGCCTCCTTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCC
  AGGCGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGA
  TCGTCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGG
  GACCTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGA
  TCCTTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAA
  GGGTGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAAC
  AACATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGC
  TGAGCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGT
  GTCCCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGC
  CTTCAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAAC
  ACCTGCAGCAGCTGGATGCCAGGATCAGTCTGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302618
• Insert Size: 3468 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302618.1, NP_001289547.1
• RefSeq Size: 5438 bp
• RefSeq ORF: 3468 bp
• Locus ID: 12265
• UniProt ID: P79621
• Cytogenetics: 16 A1
• Gene Summary: This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4).